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유카 추출물 첨가가 In Vitro 반추위 미생물 발효에 미치는 영향
황일환,이상석,Chalermglin, Piya,홍승희,최유지,하종규 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.5
An in vitro experiment with mixed ruminal microorganism was conducted to investigate the effects of Yucca extract(YE) and substrate on medium pH, gas production, number of bacteria and protozoa, enzymatic activities, and VFA at different incubation times. The factorial design consisted of 2 main factors(substrates: 1㎜ ground straw and 4mm cracked corn) with 3 minor factors(YE levels: 0, 30 and 90 ㎎) in 5 incubation times(0, 12, 24, 48 and 72 hr). All incubations were repeated three times. The supplementation of YE generally reduced gas production, ammonia concentration, the number of bacteria and protozoa and DM digestion with minor effect on medium pH and VFA production. Compared to rice straw as a substrate, corn produced more gas, decreased medium pH. increased bacterial numbers, and was more digestible. Current results indicate that YE has direct and indirect effect on the types and numbers of ruminal microbes and hence on ruminal fermentation
Advances in hot-melt extrusion technology toward pharmaceutical objectives
황일환,강신양,박준범 한국약제학회 2017 Journal of Pharmaceutical Investigation Vol.47 No.2
Hot Melt Extrusion (HME) technology is a widely used for manufacturing process in the plastic and pharmaceutical industries and is an efficient and simple for the production of a solid dispersion. This technique is an environmental friendly, continuous process that does not use solvents. It is time and cost efficient and can be easily scaled up. In addition, HME may be used in conjunction with other technologies to effectively increase the solubility and dissolution rate of poorly water-soluble drugs. Numerous research papers on the progress of HME technology in the pharmaceutical industry have been written, and products manufactured using HME have been approved by the FDA. However, there are some drawbacks to the products manufactured using through HME. These are related to the high energy input coming from the applied shear forces and high temperature, which could lead to drug or polymer degradation and thus significantly impact the product quality. Despite these disadvantages, HME has been employed in various advanced applications, such as taste masking and targeted drug delivery. This review article focuses on advances in HME technology, which include improvement in the weaknesses of HME, patient-centric formulations, and the outlook for uses such as formulation development.
황일환,Hwang, Il-Hwan 한국단미사료협회 2004 사료산업 Vol.2 No.11
전 세계적으로 사료산업에서의 유기산의 사용은 이미 보편화되어졌으며, 지속적으로 그 사용범위가 확대되고 있는 상황이다. 특히 유기산제는 이유 자돈에서의 이유 스트레스 및 사료 배합시의 문제점들을 보완하기 위한 목적으로 오랫동안 사료에 적용되어져 왔다. 또한 최근들어 육성, 비육돈과 모돈을 비롯하여 양계 및 축우까지 유기산제 적용에 대한 관심이 점차 커지고 있는 상황이다. 최근 들어서는 안전하고 깨끗한 축산물에 대한 소비자들의 요구가 커지면서, 사료내 성장 촉진목적의 항생제를 대체할 물질로서 유기산제의 중요성이 크게 대두되고 있다. 이는 유기산이 지니는 다양한 생리적, 화학적 기능들로 인한 것인데, 본고에서는 유기산이 지니고 있는 이러한 특성들과 이들이 가축의 생산성 증진에 어떠한 역할을 하는지에 대해 개략적으로 살펴봄으로써, 유기산에 대한 올바른 이해에 도움이 되고자 한다.
황상미,Cha-ja See,최정은,김선영,김정아,최규태,권지석,박시내,임경옥,오일환,이동순 생화학분자생물학회 2013 Experimental and molecular medicine Vol.45 No.12
The cytogenetic analysis of mesenchymal stromal cells (MSCs) is essential for verifying the safety and stability of MSCs. An in situ technique, which uses cells grown on coverslips for karyotyping and minimizes cell manipulation, is the standard protocol for the chromosome analysis of amniotic fluids. Therefore, we applied the in situ karyotyping technique in MSCs and compared the quality of metaphases and karyotyping results with classical G-banding and chromosomal abnormalities with fluorescence in situ hybridization (FISH). Human adipose- and umbilical cord-derived MSC cell lines (American Type Culture Collection PCS-500-011, PCS-500-010) were used for evaluation. The quality of metaphases was assessed by analyzing the chromosome numbers in each metaphase, the overlaps of chromosomes and the mean length of chromosome 1. FISH was performed in the interphase nuclei of MSCs for 6q, 7q and 17q abnormalities and for the enumeration of chromosomes via oligo-FISH in adipose-derived MSCs. The number of chromosomes in each metaphase was more variable in classical G-banding. The overlap of chromosomes and the mean length of chromosome 1 as observed via in situ karyotyping were comparable to those of classical G-banding (P¼0.218 and 0.674, respectively). Classical G-banding and in situ karyotyping by two personnel showed normal karyotypes for both cell lines in five passages. No numerical or structural chromosomal abnormalities were found by the interphase-FISH. In situ karyotyping showed equivalent karyotype results, and the quality of the metaphases was not inferior to classical G-banding. Thus, in situ karyotyping with minimized cell manipulation and the use of less cells would be useful for karyotyping MSCs.