한국족보연구서설(韓國族譜硏究序說)

황운용 ( Woon Yong Hwang ) 東亞大學校附設 石堂傳統文化硏究院 1985 石堂論叢 Vol.10 No.-

After the early phases of scholastic inquiry on the books of geneolgy in Korea, accumulation of research results was made possible and scholars began investigating the contents of the books. The changes seen in the study of geneology is assumed to have occurred because the books were considered having values as historical materials that are very important in studying Korean history. Therefore, the author of this writing investigated the literature relating geneological study thoroughly and bases on the results of this investigation developed a theory pertinent to this area. The genesis of family names and processes of family formation were thoroughly pursued. The social backgrounds from which the books of geneology came to existence were clarified under the assumption that the increase of tribal community and the expansion of the books of geneology were mutually related. Furthermore, the pride of the families implied in the books of geneology and the true nature of family names emerged through naturalization were widely explored as well as factors that influenced the books` publication that prevailed at the time. As a result of this study the author found that there had never existed any other books for Koreans, which contained as much materials as the books of geneology did. It is also found that the varieties of the contents of the books were very well contrasted with the scope of the books. The author has suggested, for future geneological study, that the maintenance and preservation plans for existing books of geneology should be carried out with substantial care. In addition, scholars involved in the study of geneology and owners of the books of geneology should work cooperatively in bringing back the historical events by excluding factors unrelated to the scholastic phenomena.

Histone 에 관한 연구 ( Ⅳ ) 사람의 Colostrum 속의 Histone

황운용(Woon Yong Hwang),이희성(Hi Sung Lee),이근배(Keun Bai Lee) 생화학분자생물학회 1974 BMB Reports Vol.7 No.1

Whole histone and histone fractions from human colostrum have been studied. The protein has been precipitated from human colostrum by adding ammonium sulfate to 60% saturation and histone fractions f1(KAP), f2a1(GRK), f2a2(LAK), f2b (KAS) , and f3 (ARE) were prepared from the precipitated protein by the procedure of johns. The resulting five major fractions have all been put on polyacrylamide disc gel electrophoresis and chromatographed for analysis of amino acid composition. The results obtained were as follows: l.. The yield of whole histone recovered was 876 ㎎ per 1000 ㎖ of human colostrum. This is very large amount as compared to the human urine and bile. 2. The relative amounts of 5 major fractions, i. e. , f1 (KAP), f2a1(GRK), f2a2(LAK), f2b(KAS), and f3(ARE) were 15.1%, 14.6, 23.1%, 42.3% and 4.9%, respectively. 3. The amounts of fractions f1(KAP), f2a1(GRK) and f2a2(LAK) from colostrum were almost similar to those of corresponding fractions of other source, marked increase in the amount of f2b (KAS) and concomitant decrease in the amount of f3(ARE) from colostrum were observed. 4. The electrophoretic mobility of histone fractions gave similar but not identical patterns to those of calf thymus and liver. 5. Amino acid analysis of the individual histone fractions showed that the over-all compositions were very similar to calf thymus and liver.

Histone에 관한 연구(IV) 사람의 Colostrum속의 Histone

황운용,이희성,이근배,Hwang, Woon-Yong,Lee, Hi-Sung,Lee, Keun-Bai 생화학분자생물학회 1974 한국생화학회지 Vol.7 No.1

저자들은 건강한 산모의 colostrum을 ammonium sulfate로 60% 포화시켜 단백질을 침전시키고, 이 단백질로부터 Johns의 방법에 의하여 ethanol과 acetone으로 처리하여 5개의 histone 분획을 얻었다. 이 분획을 polyacrylamide disc gel 전기영동 및 amino 산 조성의 분석을 행하여 사람의 요, 담즙 및 다른 동물조직에서 추출한 histone의 각 해당 분획들과 이화학적 성상을 비교 검토하여 다음과 같은 결과를 얻었다. 1. 1000 ml의 colostrum에서 876 mg의 총 histone을 추출하였다. 2. Colostrum에서 5개의 histone 주 분획, 즉 f1(KAP), f2a1(GRK), f2a2(LAK), f2b(KBS) 및 f3(ARE)의 상대적 백분율은 15.1%, 14.6%, 23.1%, 42.3% 및 4.9% 이다. 3. 분획 f2b(KAS) 및 f3(ARE)의 상대적 백분율은 다른 조직에 비하여 현저한 차이가 있다. 4. 각 분획의 polyacrylamide disc gel 전기영동상의 이동도는 다른 조직, 담즙 및 요의 그것과 거의 같다. 5. 각 histone 분획의 amino 산 조성은 다른 조직, 담즙 및 요의 그것과 별 차이가 없다. 이상과 같은 결과로 보아 colostrum에서 추출 분리한 histone 분획들은 다른 동물조직 및 사람의 담즙과 요의 histone과 동질성의 단백질로 생각된다. Whole histone and histone fractions from human colostrum have been studied. The protein has been precipitated from human colostrum by adding ammonium sulfate to 60% saturation and histone fractions f1(KAP), f2a1(GRK), f2a2(LAK), f2b(KAS), and f3(ARE) were prepared from the precipitated protein by the procedure of Johns. The resulting five major fractions have all been put on polyacrylamide disc gel electrophoresis and chromatographed for analysis of amino acid composition. The results obtained were as follows: 1. The yield of whole histone recovered was 876 mg per 1000 ml of human colostrum. This is very large amount as compared to the human urine and bile. 2. The relative amounts of 5 major fractions. i.e., f1(KAP), f2a1(GRK), f2a2(LAK), f2b(KAS), and f3(ARE) were 15.1%, 14.6%, 23.1%, 42.3% and 4.9%, respectively. 3. The amounts of fractions f1(KAP), f2a1(GRK) and f2a2(LAK) from colostrum were almost similar to those of corresponding fractions of other source. marked increase in the amount of f2b (KAS) and concomitant decrease in the amount of f3(ARE) from colostrum were observed. 4. The electrophoretic mobility of histone fractions gave similar but not identical patterns to those of calf thymus and liver. 5. Amino acid analysis of the individual histone fractions showed that the over-all compositions were very similar to calf thymus and liver.

Histone 에 관한 연구 ( Ⅶ ) 밤나무 ( Castania crenata S . et Z . ) 꽃가루의 Histone

이희성,황운용,이경효,이근배 ( Hi Sung Lee,Woon Yong Hwang,Kyoung Hyo Lee,Keun Bai Lee ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.2

It is now generally accepted that there are five main histones in the somatic cells of most species examined. However, some changes in the molecular species of animals and plants during the evolution have been reported. Recently, DeLange et al. (1971) discovered that there is a striking conservation and divergence of sequence during evolution for some of the histones between calf and pea bud. In this respect, it will be worth studying the histones of plant pollens which are unique in some species or class. We have studied on histones of some plant pollens from chestnut, pumpkin and pine tree which are distinctly related families each other. In the present studies, we report the results obtained from chestnut pollens. 1. The yield of whole histone recovered was 8.59 ㎎ per 1 g of pollens. This is very large amount as compared to those of mammary tissues. 2. The yield of DNA was 8.13㎎ per 1 g of pollens. Consequently the DNA to histone ratio was 1:1.06. 3. The relative amounts of five fractions, i.e., H1, H2a, H2b, H3 and H4 were 17.23, 19.32%, 26.89%, 23.06 and 13.50, respectively. 4. The electrophoretic mobility of individual histone fractions gave almost similar patterns to those of corresponding fractions of calf thymus. 5. We found that histone H2b fraction of chestnut pollens contained detectable amounts of ε-N-monomethyllysine. No evidence for the presence of methylated lysine or other side-chain derivatives was reported on this histone fraction. 6. Comparison of amino acid compositions reveals marked quantitative differences between corresponding chestnut pollens and pea bud histone fractions.

高麗 初期 王權考

黃雲龍 新羅大學校 1977 論文集 Vol.5 No.-

The Kingdom of Koryo had undergone many painful experiences before she could establish a central government system. King Tae-Jo, for instance, had to maintain incessant efforts to absorb the powers of the nobles who risen from various parts of Silla during her last phase. His efforts resulted in a confedrated administration body composed of royalty and nobility. Other kings after him did their own parts in strengthening royal authority by supressing the influence of the nobles. This, however, was not an easy task to accomplish. When King Tae-jo named Hye-jong as his successor, he had fo turn to the nobles for their help; and King Joeng-jong, the next king after King Hye-jong, could come to the throne only with help from the nobles in Seogyong. Royal authority, in short, was greatly influenced by the nobles. The strong powers of the nobles gradually diminished during the reign of King Kwang-jong. He did his best until he died in 975 to limit the powers of the nobility by pursuing a consistent policy of strengthening royal authority. Such strong measures as his Emancipation Law in 956, his national examination system for selecting government officials in 958 and his decree forcing government employees to wear official garments in 960 had enhanced royal authority to a great extent. Due to his strenous endeavors his successors were able to prepare the way to a central government system without many obstacles.

Isolation of Histone from Human Colostrum

이근배,이희성,황운용 생화학분자생물학회 1977 BMB Reports Vol.6 No.3

Histones are found in the nuclei of most eucaryotic cells, but they have not been found in bacteria and some fungi. Recently, the presence of histone in Neurospora crctssa has been reported. However, some workers failed to find any histone in N. crassa and in some species of fungi. As yeast is an organism that does not form condensed chromosomes during cell division, but, in contrast to bacteria, contains a well defined nucleus. The present paper deals with the isolation of basic proteins from yeast resembling histone. The methods normally used to extract histone and histone fraction from calf thymus were not specific for histories in case of the yeast. Calf thymus histone gives four major bands on polyacrylamide disc gel electrophoresis. It is of interest that although the yeast protein also shows four bands in this system, only three have positions similar to those of calf thymus. Relative mobilities demonstrated that yeast histone has a slow-moving band. Amino acid composition in which the ratio of basic amino acids was consistently less than one. The heterogeneity and molecular species between yeast and mammalian histories were also discussed.