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황우익,이성동,오수경,Hwang, Woo-Ik,Lee, Sung-Dong,Oh, Soo-Kyung 생화학분자생물학회 1982 한국생화학회지 Vol.15 No.3
우리나라 한방에서 처방되고 있는 10종 생약제를 물로 추출하여 물액기스를 암세포의 일종인 L1210, P388 및 sarcoma 180 세포 등에 대해 in vitro와 in vivo에서 screening test하여 항암 활성 및 수명연장 효과를 측정하였다. In vitro에서의 항암 활성은 각 생약제 모두(대황 제외) L1210 보다 P388 세포에서 더 높게 나타났으며 동일 세포에 대한 각 생약제의 활성은 대황과 대극이 가장 높았고 감수와 삼능, 파두의 순으로 나타났다. 또한 각 추출물을 농도별로 첨가한 후 배양 시간 별로 항암 활성을 측정한 결과 일반적으로 일정농도 이상에서 9~12시간 배양 후 세포의 감소율이 가장 높았다가 24시간 후에 는 그 수준을 유지하거나 증가되는 경향을 보였다. 이러한 세포수의 감소 원인을 규명하기 위해 추출물 첨가 후 배양시의 세포 size 분포도 변화를 농도별, 시간별로 관찰하였다. 이 결과 추출물 첨가 직후에는 size 분포가 대조군과 일치하다가 6시간 배양 후 diameter가 적은 쪽으로 이동하였다. In vivo test에서 sarcoma 180 세포를 접종시킨 swiss mice에 각 추출물을 경구적 또는 복강내 주사로 투여한 결과 경구적 투여시의 수명연장 효과는 볼 수 없었다. 복강내 주사시는 초기(50% 사망시까지)에서는 감수, 나복자, 삼능, 도안 등에서 수명연장 효과가 인정되었으나 말기(100% 사망시까지)에는 별 효과를 나타내지 못하였다. Various Korean medicinal herbs have been used for the treatment of cancer patient in the orient. This study was devised to observe the potential antitumor activity of 10 kinds of Korean medicinal herbs against L 1210 and P 388 murine tumor cells in vitro, and against Sarcoma 180 cells in vivo. Ten kinds of herbs used in this experiment are as follows; Pa-doo, Dae-geuk, Kam-soo, Won-hwa, La-bok-ja, Sam-neung, A-chool, Dae-hwang, Hoi-hyang and Do-in. The growth rate, doseresponse and size distribution of L 1210and P 388 cells cultured in the medium with or without each herb water extract were determined, and also the effects of incubation time in same medium determined. The antitumor effects of each extract were estimated by the measurement of the increased survival time of the Swiss mice inoculated with Sarcoma 180 cells in vivo test. The experimental results obtained are summarized as follows; 1. in vitro test; a) Antitumor activity of each water extract of medicinal herb was much more active in the P 388 cells than in the L1210 cells(except Dae-hwang extract). b) In the culture medium of P 388 cell, the activity (1 unit) of each extract, Pa-doo, Dae-geuk, Kam-soo, Won-hwa, La-bok-ja, Sam-neung, A-chool, Dae-hwang and Hoi-hyang was applicable to 0.14, 0.11, 0.13, 0.18, 0.62, 0.13, 0.35, 0.15 and 0.45 mg, respectively. c) In the culture medium of L 1210 cells, the activity (1 unit) of each extract was applicable to 0.22, 0.13, 0.15, 0.24, 0.93, 0.14, 0.91, 0.11 and 1.38 mg, respectively. d) The numbers of the P 388 cells were significantly decreased by incubation with each herb extract for 9 to 12 hours. e) The size distribution of P 388 cells incubated with each herb extract for 6 to 9 hours were shifed to direction of the small size region. 2. in vivo test; The survival time of Swiss mice inoculated with Sarcoma 180 cells was slightly extended at early stage (until the 50% death), compared with control group, by peritoneal injection of the extract of Kam-soo, La-bok-ja, and Sam-neung although the effects were not appeared at last stage (100% death). In the case of the oral administration of the extracts, the effects were not appeared.
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한국산 생약제로부터 항암성분의 추출 및 그의 항암성활성 측정에 관한 연구
황우익,차승만,이세영,Hwang, Woo-Ik,Cha, S.,Lee, Se-Young 생화학분자생물학회 1980 한국생화학회지 Vol.13 No.1
우리나라 한방에서 암치료제로 가장 많이 처방되고 있는 10종의 생약제 즉 삼능, 회향, 아술, 나복자, 대황, 도인 감수 파두 대극 및 원화등을 물, 알콜 및 석유에텔로 각각 추출하여 암세포의 일종인 L5178Y cell을 대상으로 in vitro 에서 각추출물의 항암활성을 측정 하였다. 이결과 수용성 추출물에서 삼능, 회향, 아술, 나복자 및 대황 등의 것은 첨가량의 증가에 비례하여 암세포의 증식이 점차적으로 억제 되었으나 감수 파두 대극 및 원화 등의 것은 첨가량의 농도에 비례하지 않고 어느 농도 이상에서는 증식이 크게 억제되고 그농도 이하에서는 억제효과가 없었다. 알콜추출물에서 상능, 회향, 아술 및 나복자 등의 것은 암세포 증식억제 효과가 없었으나 대황 감수 파두 대극 및 원화등의 것은 수용성 추출물의 활성과 같은 경향을 보였다. 석유에텔 추출물에서는 각생약제 모두 항암활성이 무시할 정도로 미약 하였다. 이상의 결과로 보아 삼능 회향 아술 및 나복자 등의 수용성 추출물중에 열에도 안정하고 비교적 독성이 적은 항암성분이 존재할 가능성이 있을 것으로 예상 된다. The number of patient with tumor is rapidly getting increase year by year, but antitumor agent has not been much developed. Therefore the studies on the development of anticancer drug is one of the most important task in the medical field. The chinese medicinal botany in Korea have been well developed since Korea Dynasty and "Dong-Eui-Bo-Gam", famous classic handbook of Korean traditional medicine, written by Huh Jun and published in 1913. In addition some medicinal plants have been used for the treatment of tumor patient. Two hundreds and nintythree kinds of medicinal herbs, which have been used in Korea, are classified according to the pharmacological effects by Hong and 10 herbs of those were to be used as anticancer agents by Cha. In this study I have attemped to obtain basic materials for the development of anticancer drugs from Korean medicinal herbs. The experimental results obtained are summarized as follows; 1. Each water-extract of Scirpus maritimus, Foeniculum vulgare, Curcuma zedoaria, Raphanus sativus and Rheum undulatum inhibited the growth of leukemia cell and the inhibitory activity was parallel to the concentration of the extract. 2. Water-extracts of Euphorbia kansui, Croton tiglium, Galarhaeus pekinensis and Daphne pseudogenkwa also inhibited the cell growth. However the effect was not proportional to the concentration, and the inhibitory effect appeared only at above of a given concentration. 3. The inhibitory effects of alcohol extracts of Scirpus maritimus, Foeniculum vulgare, Curuma zedoria and Raphanus sativus were not significant. 4. Alcohol extracts of Euphorbia kansui, Croton tiglium, Galarhaeus pekinensis and Daphne pseudogenkwa showed the similar tendency with effect of the water-extracts. 5. No inhibitory effect was observed in petroleum ether extracts of all herbs (except Euphorbia kansui). From the above results it was found that the effective antitumor component is more extractable with water than organic solvents, and the water-soluble components of Scirpus maritimus, Foeniculum vulgare, Curcuma zedoaria and Raphanus Sativus are heat-stable and less toxic. Hence it is conclusive from the present study that further experimental studies on the anticancer effect of the water-soluble components of the above 4 kinds of herbs are promising.
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인삼분의 비율별 첨가급식이 백서의 성장 및 수종 장기중 지질함량에 미치는 영향
황우익,김성미,Hwang, Woo-Ik,Kim, Sung-Mee 생화학분자생물학회 1978 한국생화학회지 Vol.11 No.4
인삼분의 비율별 첨가급식에 의한 동물의 성장 및 체성분 변동에 미치는 영향을 관찰하고자 이유직후의 체중이 94내지 100g 범위의 웅성 백서 50마리를 대상으로 하여 5종의 식이군으로 나누어 30일간 사육하연서 관찰 하였다. 식이는 대조식이와 대조식이에 인상분을 2%, 4%, 6% 및 8%씩 첨가하여 5종을 만들었고 동물은 각 해당 식이별로 5군으로 나누어 30일간 사육하면서 체중증가량, 식이 및 단백질 섭취량, 식이효율과 단백질 효율등을 측정하고 그 후 희생하여 각장기중 성분을 측정하였다. 이 결과 인삼의 첨가급식 효과는 기본식이(대조식이) 중 단백질 함량과 질에 따라 차이가 있음을 알 수 있었다. 즉 기본식이 중 단백질 함량이 낮을 경우 인삼급식효과가 양호하였고 높을수록 그첨가급식 효과는 뚜렷하지 않으며 오히려 식이효율 및 단백질 효율이 저하되었다. 한편 인삼첨가급식에 의하여 간장과 심장중 지질함량 및 total cholesteral 함량은 저하됨을 알 수 있었다. This Study was devised to ovserve the effects on the lipid metabolism and gained body weight of Albino rats by feeding of diet supplemented with 2% to 8% Ginseng root powder. The Albino rats (50 heads), weighing 94 to 104g, were divided into five diet groups, which were 1) stock diet as control, 2) 2% Ginseng diet (stock diet contained 2% Ginseng powder), 3) 4% Ginseng diet, 4) 6% Ginseng diet and 5) 8% Ginseng diet. The each group was maintained with corresponding diet for 30 days and then sacrificed. The growth rate of rats, the consumption and efficiency ratios of food and protein were determined during the feeding term, and after sacrifice of the animals, the contents of lipid and cholesterol in some organs and serum were analyzed. The results obtained are summarized as follows, 1. The growth rate was highest in the control group and decreased to order of increasing content of Ginseng powder in diet. 2. The consumptions and the efficiency ratios of food and protein were significantly decreased diet groups containing more 6% Ginseng powder than the control group. 3. Lipid contents in liver and heart were decreased by feeding of diet supplemented with 2% to 8% Ginseng powder compared to the control group. 4. The total cholesterol contents in the liver and heart were decreased by feeding of diet supplemented with 2% to 8% Ginseng powder as well as changing of the lipid contents. As the results of above, the lipid metabolism in the rat was influenced by feeding of the diet supplemented with Ginseng powder.
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마늘로부터 항암성 성분의 추출 및 그의 항암성활성 특성에 관한 연구
황우익,Hwang, Woo-Ik 생화학분자생물학회 1980 한국생화학회지 Vol.13 No.4
본 연구는 마늘 추출액이 in vitro와 in vivo에서 암세포의 중식에 미치는 영향을 관찰하여 마늘로부터 항암제 개발에 기초자료를 제공 하고자 계획하였다. 암세포는 흰생쥐의 백혈병성 임파모세포(L5178 Y cells)와 복수육종 세포(sarcoma 180 cells)를 대상으로 각각 in vitro와 in vivo에서 배양하면서 실험에 사용하였고 마늘 추출액은 실온과 $90^{\circ}C$내지 $100^{\circ}C$에서 추출한 수용성 추출물과 이들 각액에서 산(acid)에 불용성품의 제거액 및 산에 불용성분의 각 용액을 사용하여 각액의 항암성활성을 측정한 바 다음과 같은 결과를 얻었다. 1. 마늘의 실온 추출액의 활성 (L5178Y cells의 증식억제 효과)은 농도에 비례하지 않고 어느농도 이상에서야 강하였다. 2. 마늘의 가열 추출액의 활성은 농도에 비례하여 점차 증가되었다. 3. 마늘의 실온 추출액 중 산에 불용성분에는 활성이 없었으나 가열 추출액 중의 것에는 활성이 상당히 남아 있었다. 4. Sarcoma 180 세포를 접종시킨 동물에 마늘 추출액을 투여(특히 복강내 주사시)할 때 생존기간의 연장효과가 있었다. 이상의 결과로 보아 마늘의 수용성 성분중에 L5178Y cells 또는 sarcoma 180 cells등의 암세포의 증식 억제 효과가 있음을 알 수 있었다. The number of patient with tumor is rapidly getting increase year by year, but antitumor agent has not been much developed. Therefore the studies on the development of anticancer drug is one of the most important task in the medical science field. In this study I have attemped to obtain basic data for the development of anticancer drugs from the garlic. The cancer cells used were leukemic cells (L5178Y) and sarcoma 180 cells cultured in vitro and in vivo, respectively. The garlic extracts were prepared by extraction in water at room temperature and $90^{\circ}C$ to $100^{\circ}C$, and two filtrates were prepared by removing of acid insoluble part from above both extracts. The experimental results obtained are summarized as follows; 1. The garlic extract obtained at room temperature inhibited the growth rate of leukemic cells (L5178Y) in vitro. However the effect was not proportional to the concentration of the extract added, and the inhibitory effect appeared strongly at above range of a given concentration. 2. The garlic extract at $90^{\circ}C$ to $100^{\circ}C$ also inhibited the cell growth (5178Y) in vitro and the inhibitory effect was parallel to the concentration of extract added. 3. Acid insoluble part in garlic extract prepared at room temperature was shown no inhibitory effect, but that in boilled ($90^{\circ}C$ to $100^{\circ}C$) garlic extract indicated considerable inhibitory activity. 4. Survival time of Swiss mice inoculated the sarcoma 180 cells was extended by treatment with garlic extract. (specially in injection group) compared with the control group. From the above results it was found that water extracts of garlic. were shown the inhibitory effects on the growth of some cancer cells.
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황우익,이성동,오수경 ( Woo Ik Hwang,Sung Dong Lee,Soo Kyung Oh ) 생화학분자생물학회 1982 BMB Reports Vol.15 No.3
Various Korean medicinal herbs have been used for the treatment of cancer patient in the orient. This study was devised to observe the potential antitumor activity of 10 kinds of Korean medicinal herbs against L 1210 and P 388 murine tumor cells in roitro, and against Sarcoma 180 cells in vivo. Ten kinds of herbs used in this experiment are as follows; Pa-doo, Dae-geuk, Kam-soo, Won-hwa, La-bok-ja, Sam-neung, A-chool, Dae-hwang, Hoi-hyang and Do-in. The growth rate, doseiesponse and size distribution of L 1210and P 388 cells cultured in the medium with or without each herb water extract were determined, and also the effects of incubation time in same medium determined. The antitumor effects of each extract were estimated by the measurement of the increased survival time of the Swiss mice inoculated with Sarcoma 180 cells in vivo test. The experimental results obtained are summarized as follows; 1. in vitro test; a) Antitumor activity of each water extract of medicinal herb was much more active in the P 388 cells than in the L1210 cells(except Dae-hwang extract). b) In the culture medium of P 388 cell, the activity (1 unit) of each extract, Pa-doo, Dae-geuk, Kam-soo, Won-hwa, La-bok-ja, Sam-neung, A-chool, Dae-hwang and Hoi-hyang was applicable to 0.14, 0.11, 0.13, 0.18, 0.62, 0.13, 0.35, 0.15 and 0.45 ㎎, respectively. c) In the culture medium of L 1210 cells, the activity (1 unit) of each extract was applicable to 0.22, 0.13, 0.15, 0.24, 0.93, 0.14, 0.91, 0.11 and 1.38 ㎎, respectively. d) The numbers of the P 388 cells were significantly decreased by incubation with each herb extract for 9 to 12 hours. e) The size distribution of P 388 cells incubated with each herb extract for 6 to 9 hours were shifed to direction of the small size region. 2. in vivo test; The survival time of Swiss mice inoculated with Sarcoma 180 cells was slightly extended at early stage (until the 50 % death), compared with control group, by peritoneal injection of the extract of Kam-soo, La-bok-ja, and Sam-neung although the effects were not appeared at last stage (100 % death). In the case of the oral administration of the extracts, the effects were not appeared.
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