주요 약용작물에 대한 Cyanazine 제초제의 잔류 분석법

황영선,임정대,정명근 한국약용작물학회 2016 한국약용작물학회지 Vol.24 No.3

Background: Cyanazine is used as a pre-emergent herbicide once during the growing season to control weeds of many upland crops worldwide. This study aimed to establish a method to determined cyanazine residue levels in major medicinal crops by using high performance liquid chromatography-UV detection/mass spectometry (HPLC-UVD/MS). Methods and Results: Cyanazine residue was extracted with acetone from the raw products of four representative medicinal plants - Scutellaria baicalensis, Paeonia lactiflora, Platycodon grandiflorum and Angelica gigas. The extract was diluted with a large volume of saline water and directly partitioned into dichloromethane to remove polar co-extractives in the aqueous phase. It was then purifined using optimized Florisil column chromatography. HPLC analysis conducted using an octadecylsilyl column allowed the successful separation of cyanazine from co-extractives of the samples, and the amount was sensitively quantified by ultraviolet absorption at 225 ㎚ with no interference. The accuracy and precision of the proposed method were validated by conducting recovery experiments on each medicinal crop sample fortified with cyanazine at two concentration levels per crop in triplicate. Conclusions: The mean recoveries ranged from 91.2% to 105.3% for the four representative medicinal crops. The coefficients of variation were less than 10%, irrespective of the sample types and fortification levels. The limit of quantification of cyanazine was 0.02 ㎎/㎏ as verified by the recovery experiment. A confirmatory method was performed by liquid chromatography/MS using selected-ion monitoring technique to clearly identify the suspected residue.

民法改正試論 (I)

黃永善 민사법의 이론과 실무학회 1998 民事法理論과 實務 Vol.2 No.1

의료인과 의료행위

황영선 釜山醫師會 1994 釜山醫師會誌 Vol.30 No.11

MDA-MB-231 인체 전이성 유방암 세포의 침윤에 대한 Kalopanaxsaponin A의 억제 효능

황영선,박광균,박희준,정원윤 대한암예방학회 2008 Journal of cancer prevention Vol.13 No.2

Invasion and metastasis of cancer cells are the leading cause of death for cancer patients, and matrix metalloproteinases (MMPs) play an important role in these steps. In this study, we determined the effect of kalopanaxsaponin A (KPS-A), isolated from the stem bark of Kalopanax pictus Nakai, on the invasion of MDA-MB-231 human metastatic breast cancer cells. KPS-A significantly inhibited the viability and PMA-induced invasion of MDA-MB-231 cells in dose-related manner. We also found that PMA-induced invasion was suppressed by KPS-A through decreasing the MMP-9 secretion. In addition, KPS-A remarkably reduced PMA-induced DNA binding and transcriptional activity of nuclear factor-kappa B (NF-κB) and activator protein-1 (AP-1), and blocked PMA-induced phosphorylation of ERK1/2 and Akt, not p38 MAPK. Furthermore, we confirmed that PMA-induced MMP-9 activity and transcriptional activity of NF-κB and AP-1 were regulated by p38 MAPK, Akt, ERK1/2. Taken together, the suppression of MMP-9 activity through ERK1/2/AP-1 and Akt/NF-κB pathway may contribute to the anti-invasion activity of KPS-A in PMA-stimulated MDA-MB-231 cells. (Cancer Prev Res 13, 108-115, 2008)

Constitutive and Inducible Expression of Invasionrelated Factors in PC-3 Prostate Cancer Cells

황영선,Paul F. Lindholm 대한암예방학회 2015 Journal of cancer prevention Vol.20 No.2

Background:Tumor growth and invasion are interconnected with the tumor microenvironment. Overexpression of genes that regulate cancer cell invasion by growth factors, cytokines, and lipid factors can affect cancer aggressiveness. A comparative gene expression analysis between highly invasive and low invasive cells revealed that various genes are differentially expressed in association with invasive potential. In this study, we selected variant PC-3 prostate cancer cell sublines and discovered critical molecules that contributed to their invasive potential. Methods: The high invasive and low invasive variant PC-3 cell sublines were obtained by serial selection following Matrigel-coated Transwell invasion and were characterized by Transwell invasion, luciferase reporter assay, and Rhotekin pull-down assay. Lysophosphatidic acid (LPA) was added to the cultures to observe the response to this extracellular stimulus. The essential molecules related with cancer invasiveness were detected with Northern blotting, quantitative reverse transcription-polymerase chain reaction, and cDNA microarray. Results:Highly invasive PC-3 cells showed higher nuclear factor kappa B (NF-κB), activator protein 1 (AP-1) and RhoA activities than of low invasive PC-3 cells. LPA promoted cancer invasion through NF-κB, AP-1, and RhoA activities. Thrombospondin-1, interleukin-8, kallikrein 6, matrix metalloproteinase-1, and tissue factor were overexpressed in the highly invasive PC-3 variant cells and further upregulated by LPA stimulation. Conclusions:The results suggest that the target molecules are involved in invasiveness of prostate cancer. These molecules may have clinical value for anti-invasion therapy by serving as biomarkers for the prediction of aggressive cancers and the detection of pharmacological inhibitors.

무면허 의료행위의 금지규정등은 위헌인가?

황영선 釜山醫師會 1994 釜山醫師會誌 Vol.30 No.12

Comparison of the Degree of Bacterial Removal by Hand Hygiene Products

황영선 한국치위생과학회 2022 치위생과학회지 Vol.22 No.1

Background: The coronavirus disease (COVID-19) pandemic increased awareness regarding the importance of hand hygiene in infection prevention. Although social distancing and vaccination are the strongest ways to prevent infection, personal hand hygiene is the most basic and easiest way to maintain public health. However, in addition to hand washing using running water, sanitizing tissues, and disinfection products are convenient for hand hygiene, especially outdoors. Therefore, it is necessary to improve the appropriateness of individual hand hygiene methods. In this study, we investigated the degree of hand hygiene offered by various hygiene products and hand drying methods for maintaining hand hygiene.Methods: An LED UV light kit was used for fluorescent observation of hand contamination. Bacteria from the hands were cultured to compare the degree of hand hygiene offered by various hygiene products. Bacteria were cultured in a hand-shaped medium dish to identify areas vulnerable to hand hygiene. Moreover, the degree of hand hygiene was observed according to the drying method using bacterial cultures.Results: We confirmed that hand washing under running water with antibacterial soap, sanitizing with alcohol gel disinfectant, and wiping with antibacterial wet wipes was effective for hand hygiene compared to washing under running water alone. However, for all hygiene products, a large number of bacteria were detected on the fingertips. We verified that natural drying, rather than rubbing, is effective in maintaining hand hygiene.Conclusion: These results suggest that hand hygiene products and drying methods are critical in hand hygiene management. Therefore, these results provide a basis for determining whether an individual’s hand hygiene management method is appropriate.

주요 약용작물에 대한 시아나진 제초제의 잔류 분석법 개발

황영선,임정대,정명근 한국약용작물학회 2015 한국약용작물학술대회 발표집 Vol.2015 No.10

2010년 의료기관 평가 대비 중환자실 관련 지표 수행율 향상 활동

황영선,김성혜,나진선,조미정 한국의료QA학회 2009 가을학술대회 Vol.2009 No.3

Haemophilus influenzae 티올 과산화효소의 특성

황영선ㆍ김강화 전남대학교 가정과학연구소 2000 生活科學硏究 Vol.10 No.-

Haemophilus influenzae HI0571 protein that shows homology to E. coli thiol peroxidase uses yeast thioredoxin system as an electron donor[Hwang. Y. S., Chae. H. Z. and Kim. K. (2000) J. Biochem. Mol. Biol. 33, 514∼518]. The microorganism has two open reading frames, HI1158 and HI0084, showing homology to a thioredoxin reductase and a thioredoxin, respectively. Both proteins consisting thioredoxin system were expressed in E. coli, and then the proteins were subsequently purified. The H. influenzae thioredoxin system showed 5,5'-dithiobis- (2-nitrobenzoic acid) reduction activity. H. influenzae HI0751 protein, thiol peroxidase was characterized using the thioredoxin system as an electron donor. The thiol peroxidase showed the thioredoxin dependent peroxidase activity. And glutamine synthetase protection activity of thiol peroxidase against Thiol/Fe3+/O2 system was enhanced by addition of the thioredoxin. An antioxidant function of thiol peroxidase in intact cells was demonstrated by the observation that E. coli cells overexpressed with H. influenzae thiol peroxidase were less sensitive to growth inhibition by alkyl hydroperoxides.