http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Aminotransferase-catalyzed Asymmetric Synthesis of Benazepril Intermediate
황범열,차민호,박형연,김병기 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.4
Benazepril is a medication used to treat hypertension,congestive heart failure and chronic renal failures. A benazepril intermediate was synthesized through asymmetric synthesis using aromatic aminotransferase from Enterobacter sp. BK2K-1 (AroATEs). Sodium 4-methoxy-4-(2-nitrophenyl)-2-oxobutanoate (1) and (E)-4-(2-nitrophenyl)-2-oxobut-3-enoic acid (2) were tested as amino acceptors for the transamination by AroATEs. The AroATEs showed higher activity towards 1, which could be explained using a docking simulation. Both the substrate and product inhibitions for the reaction of 1 as an amino acceptor and L-glutamate as an amino donor were examined. The product inhibition by α-ketoglutarate was able to be solved by the removal of the product using the glutamate dehydrogenase (GDH) and formate dehydrogenase (FDH) coupling system. Using 50mM of 1, above 99% conversion (> 99% ee) was achieved using the AroATEs, with the GDH and FDH combined system.
김준형,이종기,윤형돈,황범열,이선구,김병기,김광,Jiwon Roh,Sui-Lam Wong 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.3
Selecting an appropriate promoter based on its expression ability is one of the most important points to consider when producing foreign proteins in a bacterial host. In this study, we compared the strength of three promoters (PaprE, PamyE, and PP4₃), all of which are widely used for foreign protein production in Bacillus subtilis. PaprE, PamyE, and PP4₃ promote the transcription of serine alkaline protease, 1,4-alpha-D-glucan glucanohydrolase, and cytidine/deoxycytidine deaminase, respectively. Among the single-copy chromosome-integrated forms of the β-galactosidase expression systems, PP4₃ demonstrated the highest expression level (200 Miller units of β-galactosidase expression), while PamyE and PaprE exhibited 70 and 10% of PP4₃-driven expression, respectively. During multi-copy (50 copies per cell) plasmid-based expression of a therapeutic model protein, staphylokinase, the order of relative strength among the three promoters was similar to that observed in the single-copy integrated system; PP4₃-driven expression yielded 120 mg/L staphylokinase.