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Aflatoxin M1 in Pasteurized Market Milk in Korea
황규춘,Joo Yea Hwang,김현욱,오미화 한국축산식품학회 2012 한국축산식품학회지 Vol.32 No.3
Aflatoxin M1, ingested as aflatoxin B1 via contaminated feedstuff and later converted into, is a major problematic target for milk safety control among the aflatoxin class. Korean government has controlled level of AFM1 in milk at 500 ppt as maximum residue level (MRL), and more recently, government also publicized the proposal for more strict control on fungal toxins about infant and baby foods. In this study the levels of Aflatoxin M1 (AFM1) of 42 marketed milk samples were determined with Enzyme-Linked Immunosorbent Assay (ELISA) to evaluate the status on the contamination of Aflatoxin M1. The evaluated ELISA performances of limit of detection (LOD) and the half maximal inhibitory concentration (IC50)were 5 pg/mL (ppt) and 49 ppt, respectively. In all 42 samples, AFM1 appeared above the 5 ppt, with the average of 21 ppt and the range of up to 90 ppt. Only 3 (7%) of samples showed the level of contamination above the EU MRL (50 ppt). Although there was incidence of higher level of contamination compared with previous reports, the result of this study requires more intensive study to control of AFM1 in milk and infant foods.
한국 오골계의 유전 특성 분석을 위한 임의 염기 서열의 Short Oligonucleotide Primer 를 이용한 Polymerase Chain Reaction 기법의 적용
황규춘,한재용,손시환,홍영호,이학교,조병욱 한국축산학회 1997 한국축산학회지 Vol.39 No.1
This study was carried out to establish the optimal RAPD condition using, short oligonucleotide primers and then to compare the genetic characteristics of Korean Native Ogol Chicken with other breed of chicken, including WL, RIR, CN and KNBC. Each 5 individuals per breed were bled and DNA was extracted from collected blood. The primers used in this study were composed of 10 oligonucleotides of 50∼80% G+C content. The optimization on the primer annealing temperature, concentration of Mg ion, amount of template DNA was conducted. From initial 400 primers, 50 polymorphic primers between individuals of same or different breeds were selected. And finally, 10 primers showing highly polymorphic and reproducible pattern were selected and 49 polymorphic markers were counted for Band Sharing Coefficient estimation. Both KNOC and KNBC showed a high genetic homogeneity (0.862, 0.858) and RIR showed most homogeneous genetic composition {0.891). In the case of interbreed comparisons, both KNOC and KNBC showed the highest genetic similarity with CN (0.810, 0.835) and the least with WL (0.772, 0.782) than any other breeds. Therefore, the RAPD technique using short oligonucleotide sequences was useful for genetic analysis in chicken
한우의 경제형질들과 관련한 유단백 유전자와 성장호르몬 유전자의 효과
이득환,황규춘,한재용,전광주,이학교,조병욱 한국축산학회 1998 한국축산학회지 Vol.40 No.2
Milk protein genes and growth hormone gene have been targeted as modulator genes in growth and carcass traits. With the objectives to detect some correlation or genotypic effect of three known functional genes on economic performance, 19 sires and their 139 progenies of Hanwoo population were genotyped for κ-casein, β-lactoglobulin and growth hormone(GH) loci by polymerase chain reaction(PCR)-restriction fragment length polymorphism(RFLP) analysis. The growth traits, such as Body Weight and Average Daily Gain, and carcass traits of this population were analysed for possible association with three genetic loci. The frequency of B allele for κ-casein was 0.37 in Hanwoo and 0.23 for β-lactoglobulin. Among the analysed loci, κ-casein gene was found most significantly associated with the growth traits of economic importance. However, the ,β-lactoglobulin and GH did not show significant effects in Hanwoo. For carcass traits, effects of κ-casein were significant in EMA. Therefore, genotyping the candidate animals for κ-casein in Hanwoo can be of great benefit in selection programs.
박영일,임경순,한재용,남경우,황규춘,박화춘 한국동물번식학회 1996 Reproductive & developmental biology Vol.20 No.2
The purpose of this study was to develop the diagnosis techniques for sex determination of rabbit embryos at preimplantation stage. To detect male specific sequences using polymerase chain reaction, two genes functional on sex determination including SRY and ZFX/Y genes were targeted using multiple oligonucleotide primer sets. Three of them for conserved SRY gene were used for appropriate amplification pattern, and then only one primer set #3 proved to be most efficient, showing male-specific strong signal ofamplified sequences. Using this male specific bandsfrom human, cattle, pig and mouse, the gender of rabbit was determined. As an another system for sex determination system, amplified 910bp fragment from ZFX/Y was digested with several restriction endonuclease and showed gender specific restriction fragments only by Hinf I. Using two different system for sex identification of rabbit in this study, blind tests for 17 samples was conducted and showed identical results from two different methods. And then, amplification limit of PCR reaction for template DNA was estimated using various amounts of DNA for both SRY and ZFX/Y systems, resulted as 20pg and 800pg, respectively. With this results, test for gender identification of rabbit embryos were performed using SRY derived amplification system. From total 22 embryos selected for its developmental state 18 were identified as male embryos, showing significant difference from expected sex ratio 1:1. This biased sex ratio was interpreted as to have been caused by the fact, reported by the fact, reported by several researchers, that male embryos develop more rapidly and are more resistant against the in vitro manipulation than female embryos.
이성진(S . J . Lee),황규춘(K . C . Hwang),최강덕(K . D . Choi),이학교(H . K . Lee),신영수(Y . S . Shin),한재용(J . Y . Han) 한국축산학회 1995 한국축산학회지 Vol.37 No.3
This study was performed to analyze genetic characteristics of Korean Native Ogol Chickens compared with various breeds of chickens including Korean Native Yellow Chickens, White Leghorns and Rhode Island Reds. For DIVA fingerprinting, we collected blood samples from each of 10 individuals per breeds and obtained the genomic DNA. The genomic DNA samples were digested with restriction enzymes(Hinf I, Hcre III. etc.) and hybridized with various probes(Jeffreys` probes 33.15, 33.6, and M13) after Southern transfer. Probe 33.1 gave only a few unresolvable bands, however, probe 33.6 and M l3 detected a large number of bands and was thought to be the desirable probes for the analysis of genetic characteristics for various breeds of chickens. The use of pooled DNA enabled us to significantly reduce the number of DNA samples for estimation of genetic relationships among breeds. Furthermore, the results of DFP prepared from pooled DNA were similar to those of DFP prepared from individual DNA. In this analysis, KNOC was estimated to be a highly inbred line (band sharing $gt; 0.69), and to be closest in genetic distance to KNYC than any other bred.
Y 염색체 특이성 DNA 분리와 단일 H-Y 항체 개발에 의한 토끼의 수정란 성 감별에 관한 연구 I. 정소를 항원으로 한 H-Y 항혈청에 의한 토끼 수정란의 성 판별
박영일,임경순,한재용,남경우,황규춘,박화춘 한국동물번식학회 1996 Reproductive & developmental biology Vol.20 No.1
This study was carried out to determine effectively the sex of rabbit embryos using H-Y antiserum. H-Y antiserum was obtained from inbred SD strain female rat which was immunized by injection of testis cell of inbred SD strain male rate into its spleen. The titer of antiserum was identified by sperm cytotoxicity test and culture of rabbit embryos with antiserum. The developed or undeveloped embryos were separated by exposure the embryos to the antiserum with H-Y antibody. Developed embryo were transferred to the recipients and sex of offspring were examined. 1. In the sperm cytotoxicity test, the rate of dead sperm showed no difference between two antisera from spleen and testis cell as antigens. It is confirmed that H-Y antibody in antiserum was absorbed by H-Y antigen in male rat spleen cells. 2. When rabbit morulae were exposed to antiserum and complement, the rate of embryos developed or arrested was 51 and 49% respectively and the rate was closely same as natural sex ratio of 50:50%. 3. When rabbit morulae were cultured for 12h in the medium containing antiserum produced by antigen of testis cell, the rate of embryos developed or arrested was 48 and 52% respectively and the rate was closely same as natural sex ratio of 50:50%. 4. Eighty rabbit embryos which were not affected by H-Y antiserum were transferred to four recipients. Two recipients were pregnant and born 13 pups among which 2 (14%) were male and 11 (86%) were female. In conclusion, existence of H-Y antibody in the serum from female rat immunized by injecting testis cell from newborn male rat to the spleen of the female rat was confirmed. When rabbitmorulae were exposed to H-Y antiserum and complement, about a half of embryos were developed to blastocysts. When the rabbit embryos not affected by H-Y antiserum were transferred, the rate of female offspring was 86%. Therefore, it was identified that most of embryos which were not affected by H-Y antiserum were female.