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인체 각질형성세포와 KB 세포에서의 Fas 항체 , IFN - γ , IL - 1α에 의한 apoptosis 유발에 관한 연구
이홍규,황규왕,박영립,김영근,( Hong Kyu Lee,Kyu Uang Whang,Young Lip Park,Young Keun Kim,David A Norris ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.2
Background: The Fas antigen is a cell surface molecule that mediates apoptosis in many cell types. Matsues group indicated that keratinocytes constitutively express the Fas antigen and apoptosis was induced only on pretreatment with interferon-r(IFN-y) in cultured normal human keratinocytes(NHK). Objective : We undertook this study to determine the induction of apoptosis by Fas antibody alone and/or in combination with IFN y, IL-1a in normal human keratinocytes(NHK) and transitional epithelioma cell lines(KB cell) which had lower levels of intracellular IL-1 receptor antago- nists(IL-1ra ). Methods : We used cultured NHK and KB cells. Each cell was treated with IFN-r, IL-la and Fas antibody for induction of apoptosis. For quantifying the apoptosis, index fluorescent DNA- binding dyes were used. Result: Fas antibody alone could induce apoptosis not only in KB cells but also in NHK cells. The combination of Fas antibody and IFN-r enhanced the induction of apoptosis in NHK and KB cells. The IL-la alone could induce apoptosis only in KB cells which had relatively small amounts of IL-1ra compared to NHK. Conclusion : Our result may indicate that Fas antigen in human keratinocytes can regulate normal epidermal cellular differentiation and proliferation. (Kor J Dermatol 1997;35(2): 273-278)