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세포주를 이용한 군소 다당류와 Glycosaminoglycan의 기능성 검색
홍유미(Yumi Hong),박시향(Sihyang Park),윤보영(Bo-Yeong Yoon),최병대(Byeong Dai Choi),최영준(Yeung Joon Choi) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.1
군소에서 정제한 GAG는 대식세포주인 RAW 264.7에서 세포독성은 보이지 않았으며 NO 생성량, TNF-α, IL-6 생성량을 증가시켜 면역조절 효과가 있음을 확인하였다. 그리고 100 ㎍/mL 농도에서 배양 72시간 후 약 40%에 가까운 위암세포(AGS) 성장 억제 효과가 나타났고, 정상세포 IEC-6에는 독성을 보이지 않았다. 이 같은 결과는 면역조절을 위한 기능성식품 혹은 위암 치료제로서 군소의 당 추출물과 GAG의 활용 가능성을 제시한다. In this study, we extracted a whole polysaccharide fraction from the sea hare, Aplysia kurodai, and screened its functional properties using cell lines. The functionalities of polysaccharide and glycosaminoglycan (GAG) were investigated with RAW 264.7 cell lines. The crude polysaccharides and GAG purified DEAE-Sepharose chromatography did not show the toxicity on RAW 264.7 cell line in the range of 10~200 ㎍/mL, whereas they increased the cell growth rate. The crude polysaccharides and purified GAG also increased the production of NO, interleukin-6 and tumor necrosis factor-α on RAW 264.7 cell. Particularly, the purified GAG inhibited the proliferation of stomach cancer cell line, AGS, up to 40% for 72 hr incubation, but not the intestinal epithelial IEC-6 cell lines.
이야기의 끝 : 톰 머피의 『웃음 없는 마을』(Bailegangaire) 연구
홍유미(Hong Yumi) 한국현대영미드라마학회 2012 현대영미드라마 Vol.25 No.1
With profound engagement with contemporary Irish concerns, Tom Murphy(1935-) is the remarkable contemporary Irish playwright who has been working for the Irish stage for almost 50 years since his first being mentioned as the most challenging playwright belonging to 'he angry young man' generation. All his works earnestly and seriously delve into the Irish psyche and national trauma related to the Irish national identity and historical memories. This paper aims to explore Murphy's Bailegangaire as the story and history of Ireland as well as those of a family by focusing on three female characters' stories: Mommo's story as 'the story of guilty feeling and healing of Ireland', Mary's as 'the story of homecoming of Ireland', and Dolly's as 'an Irish Christmas story'. Based on the Irish traditional storytelling, Bailegangaire has Mommo's endless and unfinished story at its center. Three marginalized women-Mommo, Mary and Dolly-have their own stories, which become one story with each of them representing past, present and future of Ireland. The story finally becomes a history of Ireland as well as that of their family. Through storytelling, using third person narrative, Mommo tells her own story and confesses her guilty feeling in causing the deaths of her husband and her grandson, and finally faces the reality and accepts it, which ultimately brings the end of the story. Sharing guilty feeling about her brother Tom's death, Mary helps Mommo finish her story, eventually accomplishing her own searching for home. With her extramarital pregnancy and her husband's abuse representing contemporary Ireland's broken family, Dolly's expected baby, named after Tom, becomes the symbolic figure replacing the dead Tom, thus becoming the baby Jesus figure in modern frustrated and disillusioned Ireland. In the final scene, Mommo's bed represents Ireland itself containing Mommo, Mary and Dolly with her baby in her womb as its past, present and future. In Bailegangaire, Murphy tells the story of Ireland which should confront the past and face the reality and accept the present affected by the past, hoping for the optimistic future, and shows each of the three female characters fulfills the journey of searching for home.
홍유미(Hong Yu-Mi),김광현(Kim Kwang-Hyun) 대한건축학회 2011 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.31 No.2(계획계)
The flow made by transportation and information is getting important and the transportation facilities that gathers the flow tends to increase its size. This phenomenon explains the result of functionalist approach towards the transportation facilities. However, the grounding nature of architectural materiality suggests a possible solution to the phenomenon; containment of human activity. So it is suggested that the transportation facilities should intervene the flow to create activities to obtain the urbanism. Therefore, this study will analyze the characteristics of the transportation facilities through Rem Koolhaas works to accommodate congested situations along with finding specific architectural methods of spatializing congested situations.
고아저작물 이용 활성화를 위한 확대된 집중관리제도 도입에 관한 연구
홍유미(Hong, Yu-Mi),윤종민(Yoon, Chong-Min) 충북대학교 법학연구소 2012 과학기술과 법 Vol.3 No.2
An orphan work is defined as a situation where the owner of copyrighted work cannot be identified and located by someone who wishes to make use of the work in a manner that requires the permission of the copyright owner. It is required that to use any work the user should be given consent to use it from its copyright holder. However when the identity or whereabouts of copyright holders of any orphan work is not clarified, it is difficult to be allowed to voluntarily use it. In the past the importance of orphan works was not understood properly. However with the advancement in informational technology and media, multiple numbers of works are being uploaded online and its uses are being made online too. With this shift in copyright paradigm the works fixed in the existing off line media needs to be digitalized. Because this digitalization requires copyright holders’ consent of its use, the orphan works whose copyright holder cannot be identified are emerging as a major obstacle. In addition not only the necessity of its increased utilization but also the dramatically increased demand for the derivative use of it also increases the necessity to have proper system for it. In the country, compulsory license system are already in place that allow for government authority to license works that it is satisfied are adequately identified as orphans. While useful on a small scale, as part of a broader solution for users that wish to enable mass uses, It is noticeable that the structure of the system may be too administratively burdensome on a larger. Therefore, given the characteristics of compulsory license system which requires the individual approval procedures in the usage of orphan works, Extended Collective Licensing System was examined to overcome the limitation which is not appropriate for its mass utilization. Extended Collective management means that a collecting society is given a mandate to represent the interests of all right holders even if they are not formally registered with that society. Therefor, once a contract allowing a user to use certain works is negotiated with a collecting society, the applicable national law would extend its coverage to all copyright owners beyond the known and registered members of the collective society. This way, orphan works are also included in the licence. The system also provides assurance to the users against liability for infringement.