http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 현수왕 (검색결과 5 건)
- 무료
- 기관 내 무료
- 유료
-
Aster koraiensis extract improves impaired skin wound healing during hyperglycemia
현수왕,김정현,Kyuhyung Jo,Jin Sook Kim,김찬식 한국한의학연구원 2018 Integrative Medicine Research Vol.7 No.4
Background: Diabetes mellitus (DM) is one of the most common diseases found across the world. Aster koraiensis extract (AKE) has a protective effect on diabetic complications such as diabetic retinopathy. However, the effects of AKE on hyperglycemia-linked impairment of wound healing during DM have not been elucidated. In this study, we investigated the effects of AKE on delayed wound healing induced by DM. Methods: DM was induced by intraperitoneal administration of streptozotocin (STZ; 75 mg/kg) to Sprague Dawley (SD) rats. Next, a wound was induced on the back of rats after administration of STZ. Further, AKE was prepared using an alcoholic extraction of A. koraiensis and orally administered daily for 18 days. Wound healing was evaluated using an in vitro migration assay and measuring the wound area in vivo. Skin tissue thickness was evaluated using hematoxylin and eosin staining. Matrix metalloprotease (MMP) activity and expression were detected using zymography and immunohistochemistry. Results: AKE administration improved the delayed migration of keratinocytes in hyperglycemic animals. It also attenuated an increase in keratinocyte MMP-2/9 activity induced by hyperglycemia. AKE protected against DM-induced impaired wound healing in rats and prevented the degradation of skin tissue induced by DM. In addition, AKE attenuated DM-induced increase in MMP-2/9 expression in skin tissue. Conclusions: In conclusion, AKE may promote wound healing by re-epithelization via promotion of keratinocyte migration and by attenuating the disruption of the skin tissue layer via MMP-2/9 inhibition during hyperglycemia.
-
김정현,현수왕,이익수,조규형,김영숙,김진숙,김찬식 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.2
The Aster koraiensis extract (ASKO) is a newlydeveloped dietary herbal supplement. In this study, thepotent blood glucose-lowering activity of ASKO in vitroand in vivo was investigated. In an in vitro glucose uptakeassay, ASKO was found to enhance glucose transport in3T3-L1 adipocytes. Oral administration of ASKO significantlyreduced glucose levels in normoglycemic miceduring oral glucose tolerance tests (OGTTs). In a long-termefficacy study, 4 weeks of oral ASKO treatment significantlyattenuated blood glucose levels during OGTTs indiet-induced obese (DIO) mice. ASKO also enhancedplasma insulin levels after glucose loading, leading to areduction in blood glucose levels. In addition, ASKOnormalized glucose transporter-4 mRNA expression in themuscles of DIO mice. These results indicate that ASKOhas postprandial glucose-lowering effects and could bebeneficial in the management of prediabetes or type 2diabetes mellitus.
-
국내산 벌개미취 잎 추출물의 α-glucosidase 억제능 및 항산화 활성 평가
이태구,현수왕,이익수,박봉균,김진숙,김찬식 한국약용작물학회 2018 한국약용작물학회지 Vol.26 No.5
Background: The plant Aster koraiensis has long been used as an ingredient in folk medicine. It has been reported that Aster koraiensis extract (AKE) prevents the progression of diabetes-induced retinopathy and nephropathy. However, although these beneficial effects of AKE on diabetes complications have been identified, the antidiabetic effects of AKE have not yet been completely investigated and quantified. In the present study, the glucose-lowering and antioxidant effects of aqueous and ethanolic AKEs were evaluated. Methods and Results: The glucose-lowering effects of aqueous and ethanolic (30%−, 50%−, and 80%-ethanol) AKEs were investigated via α-glucosidase inhibitory assays. The mode of inhibition by AKEs on α-glucosidase was identified through kinetic analysis. The total antioxidant capacity of each of the 4 AKEs was evaluated by assessing their conversion rate of Cu2+ to Cu+. The content of chlorogenic acid and 3,5-di-O-caffeoylquinic acid, the bioactive compounds in AKE, in each extract were analyzed by high performance liquid chromatography (HPLC). The AKEs showed potent α-glucosidase inhibitory activity with mixed inhibition mode, and significant antioxidant capacity. Conclusions: These results of this study suggested that the AKEs tested had α-glucosidase inhibitory and antioxidant effects. Among the extracts, the 80% ethanol extract showed the most significant α-glucosidase inhibitory activity, with a half maximal inhibitory concentration (IC50 value) of 1.65 ± 0.36㎎/㎖ and a half maximal effective concentration (EC50 value) for its antioxidant activity of 0.42 ± 0.10㎎/㎖. It can therefore be used as a source of therapeutic agents to treat diabetes patients.
-
고 삼투압 유발한 사람 각막세포에서 호장근 열수 추출물의 효능 실험
박봉균,조규형,현수왕,김진숙,이태구,김찬식 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Dry eyes are caused by highly increased osmolarity of tear film, inflammation, and apoptosis of the ocular surface. Polygonum cuspidatum is a herbaceous perennial plant of the genus Polygonum found in Asia and North America. However, the effects of P. cuspidatum aqueous extract (PCE) on hyperosmolarity-induced inflammation and apoptosis in human corneal epithelial cells have not been examined. Methods and Results : Hyperosmotic media induced human corneal epithelial cell (HCEC) cytotoxicity though increased inflammation, apoptosis, and oxidative stress. PCE treatment significantly inhibited expression of cyclooxygenase-2 and inflammatory cytokines (interleukin-6 and tumor necrosis factor-α), and activation of NF-κB p65 in hyperosmolar stress-induced HCECs. In addition, Hyperosmolarity-induced increase in BAX expression and activation of cleaved poly (ADP-ribose) polymerase and caspase 3 were attenuated in a concentration-dependent manner by PCE. PCE treatment restored anti-oxidative proteins such as Heme oxygenase-1 (HO-1), Superoxide dismutase-1 (SOD-1), and Glutathione peroxidase (GPx) in hyperosmolar stress-induced HCECs. Conclusion : PCE protected against hyperosmolar stress-induced inflammation, apoptosis, and oxidation by inhibiting expression of COX-2, BAX, MMP9; activation of NF-κB, caspase 3, and PARP; and increasing expression of MUC4 and anti-oxidative proteins. Overall, our data provide insight into the protective effects of PCE as a candidate for eye health.
-
DBpia
ScienceON연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
