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허향숙,Hyun-Kyu Kim,권석태 한국분자세포생물학회 2002 Molecules and cells Vol.13 No.2
The gene encoding Aquifex pyrophilus (Apy) pyrophosphatase was cloned and sequenced. The deduced amino acid sequence of Apy pyrophosphatase showed a 94.2% homology to Aquifex aeolicus (Aae) pyrophosphatase. The gene exhibits a difference in the codon usage at the third position from Aae pyrophosphatase. The gene was expressed under the control of a tac promoter in E. coli. The recombinant Apy pyrophosphatase was purified 18.7-fold with a 52.8% yield and a specific activity of 26.2 U mg−1 protein. The native enzyme has a homotetramer of 177 amino acids. The enzyme shows optimal activity in pH 7.5. The optimum temperature was approximately 70°C. A divalent cation was absolutely required for the enzyme activity; Mg2+ was the most effective.
B형 간염바이러스 표면항원 검색을 위한 Latex agglutination 법에 대한 고찰
허향숙 ( Hyang Suk Hur ),이경원 ( Kyng Won Lee ),양용석 ( Yong Suk Ryang ),송경순 ( Kyung Soon Song ),이삼열 ( Samuel Y. Lee ) 대한임상검사과학회 1985 대한임상검사과학회지(KJCLS) Vol.17 No.1
The study subjects consisted of positive blood serum samples from 151 patients and negative blood serum samples from 56 patients referred for HBsAg test by ELISA in Yonsei University Yeong Dong Hospital during 5 month period from December, 1984 to April, 1985. HBs Ag was tested by latex agglutination, using the original (LA-A) method and the recently developed, modified (LA-B) method separately as well as RPHA. The results were as follows: 1. Of the 151 HBs Ag positive samples confirmed by ELISA, 140(92.7%) were confirmed positive by RPHA, 134(88.7%) by LA-A and 135(89.4%) by LA-B (P>0.05). 2. Of the 56 HBs Ag negative samples confirmed by ELISA 55(98.2%) were shown negative by RPHA, 53(94.6%) by LA-A and 52(92.9%) by LA-B (P>0.05). 3. Of the 142 moderate to strongly reactive samples (O.D>2.0) confirmed by ELISA, 139(97.9`` %) were confirmed positive by RPHA, 133(93.7%) by LA-B (P>0.05). Of the 9 weakly reactive samples (O.D<2.0) reported by ELISA, 1(1 1. 1%) each was shown positive by RPHA and LA-A and 2(22.2%) by LA-B (P>0.05). 4. Comparison of the original latex agglutination (LA-A) and the modified latex agglutination (LA-B) methods showed their predictability levels were 97.7% by LA-A and 97.1 % by LA-B. There was no significant statistical difference between the LA-A and LA-B methods(P>0.05) and a further advantage of the modified method is its economical use of reagents. On the basis mentioned above, we considered these techniques very useful detection methods because both LA-A and LA-B appear to be equivalent about predictability. Also they can be. applied for mass screening of HBs Ag, and as routine tests for private clinics and emergency testing of HBs Ag in blood banks.