http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
반묘 BuOH층의 U937세포주에 대한 apoptosis유도 효과
허정은(Jeong Eun Huh),윤택준(Taek Joon Yoon),이종수(Jong Soo Lee),정진홍(Jin Hong Jung),김성훈(Sung Hoon Kim) 大韓藥學會 2001 약학회지 Vol.45 No.5
Mylabris phalerata (MP) is an insect that has been used for the treatment of cancer in oriental medicine. To evaluate the anticancer activity of Mylabris phalerata, We measured the cytotoxicity of Mylabris phalerata solvent fractions such as MC, EA, BuOH and residual layers on U937, human monocytic leukemia cells. Of those fractions BuOH layer of Mylabris phalerata was the moat effective with IC50 of 140 μg/ml. It effectively caused DNA fragmentation from the concentration of 50μg/ml, showed apoptotic nucleus by tenet assay and expressed apototic portion stained by Annexin-V It also induced the activation of caspase-3 and cleavage of the substrate poly (ADP-ribose) polymerase (PARP). These results suggest BuOH layer of Mylabris phalerata exerts anticancer activity by induction of apoptosis via activation of caspase-3 protease.
허정은(Huh Jeong Eun,許禎恩) 일본어문학회 2015 일본어문학 Vol.70 No.-
本論文は、旧注から新注にかけての『伊勢物語』の読解の変遷を探り、さ らに新注への新たな読みを示したものである。『伊勢物語』の第二段の解釈を考察してみると、まず、すべての登場人物に実名をあてる古注を批判する旧注であるが、「昔男」においては、古注の影響を受け、業平にあてて読むことがわかる。それで、この時期は「昔男」と業平とを完全に区別せず読むことと「昔男」と業平とをはっきり区分して読むという二つの観点を確認することができた。 次に、旧注の傾向は『伊勢物語』の背景の矛盾とか合わないところに解明 することを中心せず、物語の表現をそのまま吟味しようすることに比べ、新注は確かな注釈を定立しようとすることによって、作者がどのように 『伊勢物語』の背景などを虚構の世界に作り出されていたかというところを注目しながら注釈している。 こういう視点への変化を通して、注釈時代の転換期というのは、前時代 に見逃されてきた新たな読みや方向性を提示する、『伊勢物語』の注釈史において意味深い時期であることが確認できた。 以上のように、旧注から続く既存の伝統的読みの世界を、新注の注釈書 に至っては、独自の読みの世界を構築し得ており、『伊勢物語』注釈を通して、多角の観点に照らして『伊勢物語』を読むことができたと言える。
백서 심근에서 plasmid 벡터를 이용한 유전자 전달 및 VEGF 유전자 발현의 특성
정진옥 ( Jin Ok Jeong ),박선진 ( Sun Jin Park ),허정은 ( Jeong Eun Huh ),정은아 ( Eun Ah Jung ),오주현 ( Ju Hyeon Oh ),권현철 ( Hyeon Cheol Gwon ),이영주 ( Young Joo Lee ),김선영 ( Sun Young Kim ),박정의 ( Jeong Euy Park ),이원로 대한내과학회 2001 대한내과학회지 Vol.60 No.1
N/A Background : The purpose of this study was to compare gene expression among newly designed eukaryotic expression vectors, and to characterize the pattern of vascular endothelial growth factor(VEGF) expression using the most potent plasmids DNA vector. Methods : After exposure of a beating rat heart (Sprague-Dawley, 250-300g), 5 different types of plasmid DNA was injected directly into the myocardium. Reporter protein was analyzed by ELISA in the extracted heart. Results : The vector harboring cytomegalovirus (CMV) promoter and enhancer induced the strongest expression of reporter gene (chloramphenicol acetyl transferase; CAT) compared to those of pC3.1, pEF1a, RSV, pActin in the rat heart via direct injection of plasmid DNA into the apex (p<0.001). Using pCN-CAT, gene expression showed a dose-dependent response over a range of 0.3-10 ㎍. CAT expression could be detected up to 30 days after 10 ㎍ of pCN-CAT injection with the maximal expression on day 5. In X-gal staining of injected pCN-lacZ gene, β-galactosidase was found only around the needle track in the apex. The expressed hVEGF121 had biologic activity with vascular permeability assay (Miles assay) in guinea pigs. After injection of pCN-hVEGF121 into the apex of the rat heart, the expression of VEGF protein was dose-dependent over the range of 25 and 500 ㎍. VEGF expression was detected up to 14 days with its peak on day 2 after injection of 250 ㎍ of pCN-hVEGF121. When plasmid was injected into the apex of the rat heart, the expression of VEGF in the heart showed concentration gradient from the apex to the base. However, the expression of CAT was detected only in the apex. Conclusion : Plasmids vector with hCMV IE promoter/enhancer will provide clear advantages over other previously developed plasmids and the information regarding the behaviors of VEGF expression may be useful in angiogenic gene therapy of the heart.(Korean J Med 60:3-15, 2001)
퇴행성관절염(退行性關節炎) 한방치료(韓方治療)에 대(對)한 최근(最新) 연구(硏究) 동향(動向) - 임상연구(臨床硏究) 방법론(方法論)을 중심(中心)으로 -
서병관,류성룡,이송실,허정은,백용현,이재동,최도영,조윤제,김남재,박동석,Seo, Byung-kwan,Ryu, Seong-ryong,Lee, Song-shil,Huh, Jeong-eun,Baek, Yong-hyeon,Lee, Jae-dong,Choi, Do-young,Cho, Yoon-je,Kim, Nam-jae,Park, Dong-suk 대한침구의학회 2004 대한침구의학회지 Vol.21 No.3
Objective : The aim of this study was to review systemically clinical trials on the effectiveness and safety of herbal medicines in the treatment of osteoarthritis. Methods : Computerized literature searches were carried out on seven electronic databases, and hand-searching on some chinese medical journals in library of Kyung Hee Medical Center. Trial data were extracted in a standardized, predefined manner and assessed independently. Results : 1. Thirty reports of clinical trials and two reports of meta-analyses concerning herbal medicine were collected and reviewed. Among these reports three medical herbs were applied as topical medicine and others as internal medicine. 2. The western studies established NSAIDs or placebo as their control group. Five chinese reports established formulated herb pill(Ruanshnagshenjin pill) as their control group and Six did not establish a control group at all. 3. ACR was the most highly used diagnostic criteria in the western studies while the Chinese used their official criteria established by their government or the criteria of their text books. 4. 20 reports chose the Lequesne functional index, SHAQ, WOMAC OA index, AIMS, and their own unique scoring system as the criteria of analysing the effect. Others chose clinical symptoms, articular functions, and lab finding as their criteria. 5. 7 single herbs and 19 formulated herbs were studied. Among the formulated herbs, Achyranthes japonica was studied in 10 of the studies and Angelica gigantis Radix in 8, making them the most often studied herbs among the studies.
서병관,류성룡,이송실,허정은,백용현,이재동,최도영,조윤제,김남재,박동석 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-
Objective : The aim of this study was to review systemically clinical trials on the effectiveness and safety of herbal medicines in the treatment of osteoarthritis. Methods : Computerized literature searches were carried out on seven electronic databases, and hand-searching on some chinese medical journals in library of Kyung Hee Medical Center. Trial data were extracted in a standardized, predefined manner and assessed independently. Results : 1. Thirty reports of clinical trials and two reports of meta-analyses concerning herbal medicine were collected and reviewed Among these reports three medical herbs were applied as topical medicine and others as internal medicine. 2. The western studies established NSAIDs or placebo as their control group. Five chinese reports established formulated herb pill(Ruanshnagshenjin pill) as their control group and Six did not establish a control group at all. 3. ACR was the most highly used diagnostic criteria in the western studies while the Chinese used their official criteria established by their government or the criteria of their text books. 4. 20 reports chose the Lequesne functional index, SHAQ, WOMAC OA index, AIMS, and their own unique scoring system as the criteria of analysing the effect. Others chose clinical symptoms, articular functions, and lab finding as their criteria. 5. 7 single herbs and 19 formulated herbs were studied. Among the formulated herbs, Achyranthes japonica was studied in 10 of the studies and Angelica gigantis Radix in 8, making them the most often studied herbs among the studies.
Huh, Jeong-Eun,Kang, Kyung-Sun,Chae, Chanhee,Kim, Hyung-Min,Ahn, Kyoo-Seok,Kim, Sung-Hoon WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-
Cantharidin is an active compound from blister beetles traditionally used for the treatment of cancer. It is known to exert its antitumor activity by inducing apoptosis in cancer cells. However, its signaling pathway still remains unclear. Therefore, we investigated the roles of the mitogen-activated protein kinases (MAPKs) and the tumor suppressor gene, p53, during cantharidin-induced apoptosis in U937 human leukemic cells. Cantharidin effectively activated ERK-1/2, p38 and JNK in U937 cells in a time- and dose-dependent manner. Cantharidin also exhibited a strong cytotoxicity and induced apoptosis in U937 cells. For the evaluation of the role of MAPKs, PD98059, SB202190 and SP600125 were used as MAPK inhibitors for ERK-1/2, p38 and JNK. PD98059 did not affect cantharidin-induced cytotoxicity and apoptosis, whereas SB202190 and SP600125 significantly interfered with cytotoxic and apoptotic activities induced by cantharidin. Cantharidin alone induced the apoptosis by phosphorylation of p53, up-regulation of downstream target genes, MDM2 and p21 and also cleaved caspase-3, whereas SB202190 and SP600125 caused the down-regulation of p53, MDM-2, p21 and cleaved caspase-3 after a co-treatment with cantharidin. Similarly, SB202190 and SP600125 significantly disturbed the caspase-3 activity after a co-treatment with cantharidin by colorimetric assay. Taken together, these results suggest that cantharidin can induce apoptosis by activation of p38 and JNK MAP kinase pathways associated with p53 and caspase-3.