http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
胃癌 組織의 Lysozyme, Carcinoembryonic Antigen 및 Keratin에 對한 免疫 組織 化學的 硏究
이혜수,허장호,최호열 의과학연구소 1991 全北醫大論文集 Vol.15 No.1
Gastric cancer is the most common tumor in Korean. To investigate the significance of the immunohistochemical study using a immunoperoxidase technics on the formalin fixed, paraffin embedded section of the gastric cancer tissues, peroxidase-antiperoxidase stain for lyso- zyme, carcinoemvbryonic antigen(CEA) and keratin were carried out on the 68 cases of the gastric cancer of 27 cases of well differentiated adenocarcinoma, 14 cases of moderate differentiated adenocarcinoma, 22 cases of poorly differentated adenocarcinoma and 5 cases of the signer ring cell carcinoma, and 21 cases of the no- rmal gastric tissues. The results were as follows. 1. Rate of the positivity of the lysozyme on the gastric cancer was 69.1%(47/68). And rate and degree of the positivity was markedly decreased in the poorty differentiated adenocarcinoma compared with well differe- ntiated adenocarcinoma. 2. In the stain for the carcinoembryonic antigen, the rate of positivity was 83%(59/68), and moderate to st- rong positive reactions were shown independent of its differentiation. 3. Keratin in the normal and all gastric cancer tissues was not demonstrated. Above results suggest that detection of the lysozyme and carcinoembryonic antigen using a peroxidase-anti- peroxidase method are helpful to the diagnosis of the degree of differentiation of gastric cancer tissues.
아누파마 슈레스타,김은창,임춘근,조세열,허장현,박덕환 한국농약과학회 2009 농약과학회지 Vol.13 No.4
Three beneficial bacterial agents, Lactobacillus strain KLF01, Lactococcus strain KLC02 and Paenibacillus strain KPB3 were showed clear zone against plated Pectobacterium carotovorum subsp. carotovorum (Pcc) soft rot pathogen. In greenhouse test, bio-control efficacy was more significantly effective in the treatments by KLC02 and KPB3 as 64%, 50%, 56% and 66%, 57%, 58% according to date of evaluation, respectively. In case of KLF01 control effect was relatively lower than treatments of KLC02 and KPB3 but its efficacy was still significantly observed when compared to control (only water treatment). Furthermore, KLF01, KLC02 and KPB3 showed 55%, 60% and 62% bio-control efficacy, respectively in field test from early March to late July of 2009. Thus, we suggest that these strains can be useful as bio-control agents against soft rot caused by Pcc.
Shree Prasad Thapa,이효진,박덕환,김삼규,조준모,조세열,허장현,임춘근 한국식물병리학회 2009 Plant Pathology Journal Vol.25 No.4
The potential antiviral effects of the culture filtrates (CF) from Serratia marcescens strain Gsm01 against yellow strain of Cucumber mosaic virus (CMV-Y) were investigated. The culture filtrate of S. marcescens strain Gsm01 applied on Chenopodium amaranticolor showed high inhibitory activity, likewise no necrosis appeared when applied on the tobacco plants 2 days before CMVY inoculation. When plants were challenge inoculated with CMV-Y for eighteen days, the disease incidence in plants with culture filtrate of S. marcescens Gsm01 did not exceed 59%, whereas 100% of control plants were severely infected. The results of double antibody sandwich- enzyme linked immunosorbent assay (DAS-ELISA), reverse transcriptase polymerase chain reaction (RTPCR), dot blotting, and western blotting showed that culture filtrate treatment highly affected the accumulation of CMV-Y or its CP protein gene in the treated plant leaves. It was also observed that the culture filtrate had no RNase activity on genomic RNAs of CMV-Y, suggesting that culture filtrate may not contain ribosome inactivating proteins (RIPs) or proteins with RNase activity. These data shows that culture filtrate of S. marcescens strain Gsm01 seems to be a promising source of antiviral substance for the practical use.
Rosemary Shrestha,박덕환,Jun Mo Cho,조세열,Calum Wilson,황인규,허장현,임춘근 한국분자세포생물학회 2008 Molecules and cells Vol.25 No.1
The disease-specific (dsp) region and the hypersensitive response and pathogenicity (hrp) genes, including the hrpW, hrpNEp, and hrpC operons have previously been sequenced in Erwinia pyrifoliae WT3 [Shrestha et al. (2005a)]. In this study, the remaining hrp genes, including the hrpC, hrpA, hrpS, hrpXY, hrpL and hrpJ operons, were determined. The hrp genes cluster (ca. 38 kb) was comprised of eight transcriptional units and contained nine hrc (hrp conserved) genes. The genetic organization of the hrp/hrc genes and their orientation for the transcriptions were also similar to and collinear with those of E. amylovora, showing ≥ 80% homologies. However, ORFU1 and ORFU2 of unknown functions, present between the hrpA and hrpS operons of E. amylovora, were absent in E. pyrifoliae. To determine the HR active domains, several proteins were prepared from truncated fragments of the N-terminal and the C-terminal regions of HrpNEp protein of E. pyrifoliae. The proteins prepared from the N-terminal region elicited HR, but not from those of the C-terminal region indicating that HR active domains are located in only N-terminal region of the HrpNEp protein. Two synthetic oligopeptides produced HR on tobacco confirming presence of two HR active domains in the HrpNEp. The HR positive Nterminal fragment (HNΔC187) was further narrowed down by deleting C-terminal amino acids and internal amino acids to investigate whether amino acid insertion region have role in faster and stronger HR activity in HrpNEp than HrpNEa. The HrpNEp mutant proteins HNΔC187 (D1AIR), HNΔC187 (D2AIR) and HNΔC187 (DM41) retained similar HR activation to that of wildtype HrpNEp. However, the HrpNEp mutant protein HNΔC187 (D3AIR) lacking third amino acid insertion region (102 to 113 aa) reduced HR when compared to that of wild-type HrpNEp. Reduction in HR elicitation could not be observed when single amino acids at different positions were substituted at third amino acids insertion region. But, substitution of amino acids at L103R, L106K and L110R showed reduction in HR activity on tobacco suggesting their importance in activation of HR faster in the HrpNEp although it requires further detailed analysis.