파프리카 묘의 직접 정식 시 절곡 방법에 따른 생육 및 생산량

허영문,고바울,구양규,김철민,김호철,배종향,Hur, Young Mun,Ko, Baul,Ku, Yang Gyu,Kim, Chul Min,Kim, Ho Cheol,Bae, Jong Hyang 한국생물환경조절학회 2021 생물환경조절학회지 Vol.30 No.4

코이어 배지를 이용한 파프리카 재배에서 직접 정식 시 절곡방법에 따른 정식 후 초기 생육과 수확량을 비교하였다. 절곡방법은 I-type(절곡각도 0°), 옆으로 뉘인 L-type(절곡각도 90°), 뒤집어서 뿌리가 위로 향하도록 하는 U-type(절곡각도 180°)로 하였다. 줄기 신장 및 직경, 엽면적의 주간 평균 발달량은 정식 후 초기에 다른 처리구에 비해 영양생장이 왕성하였던I-type에서 가장 높았다. 정식 후 46주째의 뿌리 무게도 I-type 처리구에서 다른 처리구들에 비해 약 1.3-1.7배 정도 무거웠다. 처리구별로 330주에서 생산된 수확량은 각각 약 510, 420 및 480kg으로 I-type에서 가장 높았다. 따라서 코이어 배지를 활용한 파프리카 재배에서 직접 정식 시 정식 초기의 뿌리 활착 및 세력 확보, 그리고 이후 착과 안정성에서 I-type이 적합할 것으로 판단된다. This study aimed to compare the growth and production of paprika (Capsicum annuum) planted directly on a coir substrate slab according to the bending methods. The existing root direction was bent to 0° (I-type), 90° (L-type), and 180° (U-type), respectively. The weekly average growth such as stem length, diameter, and leaf area tended to be the highest in the I-type bending, but there was no statistical difference. Root weight at 46 weeks after planting was also about 1.3 to 1.7 times heavier in the I-type than the L- and U-type bending. As the yield produced by 330 plants by bending methods, the initial yield was the highest in the U-bending, but then the highest in the I-bending. Accordingly, the total yield was the highest in I-bending. Consequently, when planting on coir substrates directly in paprika cultivation, the I-type bending should be considered most suitable for securing root and plant vigor in the early stages of planting and for enhancing fruiting stability.

고온환경에서의 심폐기능변화에 관한 연구

허영문,양훈모,심동원 순천향대학교 1992 논문집 Vol.14 No.4

To investigate the effect of extreme high temperature on the cardiopulmonary function, we measured the heart rate, blood pressure, lung volumes, and breath-holding time before and during exposure to 70℃ environment in male adults. 1) The heart rate demonstrated exquisite sensitivity to extreme high temperature; exposure to heat increased the heart rate from 75 bpm to 124 bpm(65% increase). 2) There was a tendency for both systolic and diastolic pressure to decrease, which was not statistically significant. 3) While the exposure increased the respiratory rate from 19/min to 28/min (47% increase), it decreased the tidal volume from 566ml to 314ml. 4) The vital capacity decreased from control of 4.31 L to 3.26 L during exposure. The exposure also decreased the maximum breathing capacity (before exposure, 99 L; during exposure, 56 L0. 5) The breath-holding time decreased from 48 sec to 38 sec during exposure.

뇌 해마의 배측 부분이 제거된 흰쥐에서 선조체기저핵과 중격측좌핵의 아미노산 분석

허영문,김종규,민영기 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.1

배측 해마와 복측 해마의 기능 분화가 있는지 또는 선조체기저핵과 중격측좌핵이 해마의 영향을 받아 아미노산 신경전달물질에 어떠한 영향을 미치는지를 알아보기 위하여 대뇌피질을 거쳐 양쪽 해마의 배측 부위만을 제거한 해마군과 해마를 덮고 있는 대뇌피질 부분만을 제거한 대뇌피질 대조군, 그리고 정상 대조군을 마련한 다음, high performance liquid chromatograph (HPLC)를 이용하여 orthophthalaldehyde (OPA) precolumn 유도체화법으로 선조체기저핵과 중격측좌핵 조직에서 여러가지 아미노산 함량을 측정하여 다음과 같은 결과를 얻었다. 1. 선조체기저핵의 아미노산 함량 : 배측 해마군의 threonine과 alanine의 함량은 정상 대조군 및 대뇌피질 대조군의 값사이에서 모두 유의하게 감소되었으나, 정상 대조군과 대뇌피질 대조군의 값사이에는 유의한 차가 없었다. 그리고 나머지 aspartate, hgutamate, serine 및 glycine의 값에는 세군 사이에 이렇다 할 차가 없었다. 2. 중격측좌핵의 아미노산 함량: 배측 해마군의 glutamate와 alanine의 함량은 정상 대조군 및 대뇌피질 대조군의 값사이에서 모두 유의하게 감소되었으나, 정상 대조군과 대뇌피질 대조군 사이에는 유의한 차가 없었다. 그리고 나머지 aspartate, serine, glycine 및 threonine의 값에는 세군 사이에 이렇다 할 차가 없었다. 이 실험 결과를 볼 때 선조체기저핵은 해마의 복측 부위보다 배측 부위의 영향을 더 많이 받으며, 선조체기저핵의 threonine과 alanine은 억제적 신경전달물질로 작용하는 것으로 보이며, 기능적인 면에서 선조체기저핵은 변연계 보다는 오히려 기저핵(basal ganglia)과 더 깊은 관계가 있는 것으로 생각된다. 중격측좌핵도 해마의 복측 부위보다 배측 부위의 영향을 더 많이 받으며, 중격측좌핵의 glutamate는 촉진적 신경전달물질로 작용하고, alanine은 억제적 신경전달물질로 작용하는 것으로 생각된다. 따라서 중격측좌핵은 변연계와 더 깊은 관계를 가지며, 해마는 기능적으로도 분화가 되어있는 것으로 추리된다. In order to investigate that the free amino acids一glutamate, aspartate and other free amino acids might function as hippocampal neurotransmitters we analyzed the amino acid contents in the nucleus fundus striati and the nucleus accumbens septi tissue of the hippocampectomized female rats by high performance liquid chromatograph (HPLC). Thirty female rats, weighing about 230 g at the time of operation, were divided into 3 groups. Female rats that had their hippocampal tissue anterodorsal to the hippocampal flexure removed bilaterally through overlying cortex surved as a dorsal hippocampal group, rats that had only their cortical tissue overlying the hippocampus removed bilaterally as a cortical control group, and normal rats as a normal control group. After a survival period of 7 days, the animals were sacrificed by decapitation. Two to three milligrams of tissue were removed from the nucleus fundus striati and the nucleus accumbens septi in one side of the brain. Content of glutamate, aspartate and other free amino acid were measured using HPLC. 1. In the nucleus fundus striati, the contents of threonine and alanine were reduced significantly in the hippocampal group than in the normal control ( P < 0.001, P < 0.001 ) and the cortical control group ( P < 0.001, P < 0.001 ), while there were no significant differences between the 2 control groups. The differences in the contents of aspartate, glutamate, glycine and serine among the 3 groups were nonsignificant. Ablation of the dorsal hippocampus reduced the contents of threonine and alanine in the nucleus fundus striati. 2. In the nucleus accumbens septi, the contents of glutamate and alanine were reduced significantly in the hippocampal group than in the normal control group ( P < 0.001, P < 0.001 ) and the cortical control group ( P < 0.001, P < 0.001), while there were no significant differences between the 2 control groups. The differences in aspartate, serine, glycine and threonine among the 3 groups were nonsignificant. Ablation of the dorsal hippocampus reduced the contents of glutamate in the nucleus accumbens septi. Ablation of the dorsal hippocampus reduced the contents of alanine in the nucleus accumbens septi. From the above results it is suggested that there is a functional differentiation of the hippocampus.

고장식염수가 토끼 빈창자 점막 산호성 과립세포의 미세구조에 미치는 영향

문정석,윤상필,정윤영,허영,장인엽,김종중 朝鮮大學校 附設 醫學硏究所 1998 The Medical Journal of Chosun University Vol.23 No.1

This experiment was performed to study the influence of hypertonic saline on the production of the secretory granules by the cellular organelles of the jejunal Paneth cells of the rabbit fed on the 3 % hypertonic saline. The jejunal mucosae of the experimental rabbits were removed partially in the upper one third of the jejunum and fixed in 2.0% glutaraldehyde for 2 hours, followed by 1% osmium tetroxide fixative for 2 hours. Then they were embedded in epon mixture, cut with ultramicrotome and done uranyl acetate-lead citrate double stain, and observed with JEM100 CX Ⅱ electron microscope. Observed results were as follows: 1. The number of secretory granules of a higher electron density was gradually increased as a whole in the course of time after feeding on the hypertonic saline. 2. After feeding on the hypertonic saline, secretory granules of various sizes with a higher electron density mostly occupied the supranuclear region of Paneth cells, but a few secretory granules of various sizes with low electron density were observed. 3. After feeding on the hypertonic saline, the Goigi complex, the mitochondria and the rough surfaced endoplasmic reticula were gradually figured, especially the cisternae of the rough surfaced endoplasmic reticula displayed polyhedral shapes. 4. Considering the above findings, following the feeding on the hypertonic saline, the function of the jejunal Paneth cells was influenced and secretory granules of various sizes with a higher electron density were more abundant as compared with those of a lower electron density. From the above results, it was considered that the Golgi complex, the mitochondria and especially the rough surfaced endoplasmic reticula were activated to maintain homeostasis. As a result of that, the production and concentration of secretory granules were prominently observed.

굴, Crassostrea gigas 부착기 유생의 부착에 미치는 신경전달물질종의 영향

허영백,조규태,변순규,문태석 한국패류학회 2014 The Korean Journal of Malacology Vol.30 No.4

We determined the effects of neuroactive compounds known as synthetic larval settlement inducers on the settlement of the Pacific oyster C. gigas pediveliger on the larval collector. Six types of the inducers, serotonin (5-HT), γ-amino butyric acid (GABA), L-3,4-dihydroxyphenylalanine (L-DOPA), norepinephrine, epinephrine and methyl bromide (MB) were tested. All the chemicals induced larval settlement, MB being the most effective with settlement rate of 42.7 ± 2.7%, followed by GABA (35.4 ± 2.0%), 5-HT (29.1 ± 2.2%), L-DOPA (19.2 ± 2.1%), epinephrine (15.2 ± 0.9%), and norepinephrine (11.0 ± 1.2%). The chemicals γ-amino butyric acid and methyl bromide were also better in terms of settled density on the collector with their respective density of 1.97 ± 1.42 and 2.37 ± 1.86 ind/cm2, reminiscent of being most effective candidates for a larval settlement inducer in the oyster hatchery. 굴 부착기 유생의 부착 유인 효과를 조사하기 위하여 6종류(serotonin, γ-amino butyric acid, L-3,4-dihydroxyphenylalanine, norepinephrine, epinephrine, methyl bromide) 의 신경전달물질을 채묘기질에 직접 처리하여 굴 부착기 유생의 부착 유인 효과를 조사하였다. 조사결과 평균 채묘율는 각각 29.1 ± 2.2%, 35.4 ± 2.0%, 19.2 ± 2.1%, 11.0 ± 1.2%, 15.2 ± 0.9% 및 42.7 ± 2.7%로 MB 처리구에서 가장 높은 결과를 보였고, norepinephrine 처리구에서 유의적으로 가장 낮은 결과를 보였다 (P < 0.05). 채묘기질 cm2당 부착밀도와 부착빈도는 γ -amino butyric acid와 methyl bromide 처리구에서 각각1.97 ± 1.42 및 2.37 ± 1.86 마리/cm2로 가장 높은 부착밀도를 보였다 (P < 0.05). 본 실험결과 굴 인공종묘배양장 등에서 부착기 유생의 부착요율을 높이는데, γ-amino butyric acid와 methyl bromide를 이용하면 효과적일 것으로 보인다

멍게 어미 유래에 따른 인공종묘 중간육성 시험

허영백,지영주,문태석,김병학,조필규,임영섭 한국수산과학회 양식분과 2010 한국수산과학회 양식분과 학술대회 Vol.2010 No.6

고장식염수가 토끼 빈창자 점막 산호성 과립세포의 미세구조에 미치는 영향

문정석,윤상필,정윤영,허영,장인엽,김종중 조선의대 1996 The Medical Journal of Chosun University Vol.21 No.2

미더덕, Styela clava의 인공채묘에 관한 연구

조필규,허영백,김정우,임영섭,문태석,조기채,김병학 한국수산과학회 양식분과 2010 한국수산과학회 양식분과 학술대회 Vol.2010 No.6

흰쥐 삼차신경중간뇌핵에 관한 전자현미경적 연구

정윤영,허영,윤상필,장인엽,김종중,문정석,배춘상 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.4

인공수정시술주기에서 내인성 LH, GnRH Agonist 및 hCG를 이용한 배란유발후 임신율의 비교연구

이종인,허영문,전은숙,윤정임,정구성,홍기언,유승환,이현숙,홍정의,이지삼,Lee, Jong-In,Hur, Young-Mun,Jeon, Eun-Suk,Yoon, Jeong-Im,Jung, Goo-Sung,Hong, Ki-Eon,You, Seung-Hwan,Lee, Hyeon-Sook,Hong, Jeong-Eui,Lee, Ji-Sam 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.3

Objective: This study was designed to evaluate the effects of endogenous LH surge, GnRH agonist (GnRH-a) or human chorionic gonadotropin (hCG) as ovulation trigger on pregnancy rate by intrauterine insemination (IUI). Method: Patients received daily 100 mg of clomiphene citrate (CC) for 5 days starting on the third day of the menstrual cycle followed by human menopausal gonadotropin (hMG) for ovulation induction. Follicles larger than >16 mm in diameter were present in the ovary, frequent LH tests in urine were introduced to detect an endogenous LH surge. Final follicular maturation and ovulation were induced by GnRH-a 0.1 mg (s.c.) or hCG $5,000{\sim}10,000$ IU (i.m.) administration except natural ovulation. Pregnancy was classified as clinical if a gestational sac or fetal cardiac activity was seen on ultrasound. Results: There were no differences in age, duration of infertility and follicle size, but more ampules of hMG were used in GnRH-a group compared to hCG 10,000 IU treated group (p<0.05). Lower level of estradiol ($E_2$) on the day of hCG or GnRH-a injection was observed in hCG 10,000 IU group than other treatment groups (p<0.01). The overall clinical pregnancy rate was 19.8% per cycle (32/162) and 22.2% per patient (32/144). Pregnancy rate was higher in natural-endogenous LH surge group (37.5%, 9/24) than GnRH-a (18.8%) or hCG treated group (20.9% & 13.9%), but this difference was not statistically significant. No patient developed ovarian hyperstimulation. Abortion rate was 22.2% (2/9) in hCG 5,000 IU group. Delivery or ongoing pregnancy rate was 37.5% (9/24), 18.8% (3/16), 16.3% (7/43) and 13.9% (11/79) in endogenous LH surge, GnRH-a, hCG 5,000 IU and hCG 10,000 IU treatment groups, respectively. Conclusion: These results support the concept that use of natural-endogenous LH surge in stimulated cycles may be more effective to obtain pregnancies by IUI than GnRH-a or hCG administration.