Presence of a bi-directional S phase-specific transcription regulatory element in the promoter shared by testis-specific TH2A and TH2B histone genes

Huh, Nam-Eung,Hwang, Inhwan,Lim, Kyu,You, Kwan-Hee,Chae, Chi-Bom 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

During mammalian spermatogenesis, somatic histones are replaced by testis-specific variants. The synthesis of the variants occurs primarily in the germ cells undergoing meiosis in the absence of DNA replication. We have cloned the genes encoding rat somatic and testis-specific H2A (TH2A) histones. The two genes share 300 bp of 5; upstream region with respective H2B genes: somatic H2A with somatic H2B and testis-specific TH2A with testis-specific TH2B gene. The deduced amino acid sequences show that H2A and TH2A histones have eight amino acid differences in the first half of the molecules and three consecutive changes in the C-terminal region. TH2A gene is expressed only in testis. Although synthesis of TH2A and TH2B histones is independent of DNA replication and insensitive to inhibitors of DNA synthesis in testis, the regulatory region shared by the two genes contain a bi-directional S phase-specific transcription regulatory element. In addition, TH2A gene, like TH2B gene, contains the consensus sequence element in the 3' non-coding region which is involved in the S phase-specific stabilization of histone mRNA.

콩나물의 Asparagine 생합성에 관한 연구

변시명,이춘영,허남응 한국농화학회 1977 Applied Biological Chemistry (Appl Biol Chem) Vol.20 No.1

Asparagine biosynthesis by soybean sprouts grown under the dark conditions has been demonstrated. The amount of free asparagine synthesized in ten day-old soybean sprouts. increases to 22.7 on the dry weight base. The effects of nitrogen compounds such as NH₄Cl, (NH₄)₂SO₄ and urea on asparagine synthesis during the sprouting were examined and the results showed that urea was more effective than other two compounds. Glutamine-dependent asparagine synthetase was partially purified (8.6 folds) through ammonium sulfate fractionation, followed by Sephadex G-150 gel filtration. The enzyme was very labile and required protection by thiol groups or high level of glycerol. The mixture of ATP and Mg^(++) ion also stabilized the enzyme activity. The enzyme utilized glutamine more effectively than NH₄^+ as an amide donor for the formation of asparagine. The enzyme required L-aspartate (Km=3.1 mM), L-glutamine, ATP and Mg^(++). It showed pH optimum of 7.5 and catalyzed the formation of β -aspartyl hydroxamate in the presence of L-aspartate, ATP, Mg^(++) and NH₂OH in the reaction mixture.

잿빛곰팡이병균 Botrytis cinerea가 분비하는 Polygalacturonase의 부분정제와 특성

나유진,김재원,정영륜,허남응,조광연 한국식물병리학회 1994 Plant Pathology Journal Vol.10 No.3

잿빛곰팜이병균 Botrytis cinera가 분비하는 Polygalacturonase의 부분정제와 특성

나유진,김재원,정영륜,허남응,조광연 한국식물병리학회 1994 Plant Pathology Journal Vol.10 No.3

Polygalacturonase (PG) produced by Botrytis cinerea in the culture broth containing citrus pectin as a carbon source was partially purified and characterized. PG was produced on a range of carbon sources such as starch, glycerol, cellobiose, and Na+-PAG with total activities of 34.8, 32.0, 29.2, 27.8 units, respectively. The specific activity was highest with 2316.7 units on Na+-PGA. Proteins of culture filtrate were concentrated with polyethylene glycol and acetone and applied to a hydroxyapatite column. Among three active fractions collected from the column, the reaction containing the highest PG activity was resolved by a Q-sepharose column. The active fraction from the Q-sepharose column was further purified by HPLC Mono Q column. The partially purified enzyme was analyzed by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Among a few protein bands revealed, the amount of the protein of which molecular weight estimated to be 43 kDa coincided with the PG activity. The partially purified PG had optimal temperatures between 35~55$^{\circ}C$ and pH between 4.5~5.5.

Glycine betaine의 급여가 돈육의 품질에 미치는 영향

박구부,허선진,양한술,이정일,곽석준,이중동,허남응,김진성,주선태 한국동물자원과학회 2004 한국축산학회지 Vol.46 No.5

Isoeugenol의 넙치(Paralichthys olivaceus)에 대한 진정, 마취 및 독성효과 검토

신기욱,신용승,김영림,이은영,양향희,팔락샤,허남응,연성찬,이희천,오명주,조성준,강승원,정태성,Shin, Gee-wook,Shin, Yong-seung,Kim, Young-rim,Lee, Eun-young,Yang, Hyang-hee,Palaksha, K.J.,Huh, Nam-eung,Yeon, Seong-chan,Lee, Hee-chun,Oh, Myung- 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.2

Olive flounder (Paralichthys olivaceus) is the major mariculture fish in Korea. The annual aquaculture production of olive flounder in Korea during the period of 2003 was 300,000 ton (2002 Statistics, Ministry of Maritime Affairs & Fisheries, Korea Government). Anesthetics is very necessary in aquaculture to minimize stress and damage during harvesting, grading, transportation, spawning induction and handling to fish. In the present study, isoeugenol as new anesthetic in marine fish, especially olive flounder, was examined to know the efficacy and proper concentration. As a result, olive flounder adult was exhibited sedation at 5 ppm at 10 and $15^{\circ}C$, and 7.5 ppm at $20^{\circ}C$, respectively. Anesthesia was required at least 10, 7.5 and 10 ppm at $10^{\circ}C$, $15^{\circ}C$ and at $20^{\circ}C$, respectively. In case of fry, the effect of sedation was observed from 2.5 ppm at $10^{\circ}C$ and 5 ppm at 15 and $20^{\circ}C$, respectively. Anesthesia was observed from 2.5 ppm at $10^{\circ}C$, 5 ppm at 15 and $20^{\circ}C$, respectively. In acute toxicity test, it was impossible to explore $LD_{50}$ with the concentration of isoeugenol adult at $15^{\circ}C$ used, but over immersion volume of 15 ppm at $15^{\circ}C$ was observed mortality in fry. Based on the present study, isoeugenol was identified as a safe and active anesthetic to olive flounder.

Experimental evaluation of pathogenicity of Lactococcus garvieae in black rockfish(Sebastes schlegeli)

정태성,강성현,신기욱,신용승,Palaksha K.J.,김영림,양향희,이은영,이응구,허남응,주오명 대한수의학회 2004 Journal of Veterinary Science Vol.5 No.4

Black rockfish (Sebastes schlegeli) is an important mariculture species in Korea. The production of this fish is drastically declined due to bacterial diseases, particularlystreptococcosis caused by Lactococcus garvieae. The bacterial surface characteristics of SJ7 and TY6 werefound to have capsule but not NB13 and YS18. The experiential evaluation of L. garvieae pathogenicity, the capsular isolates showed high cumulative mortality i.e. SJ7 (100%) and TY6 (60%) compared to non-capsular isolates. Based on this result the capsular isolates L. garvieae were highly suspected as the causative agent ofstreptococcosis in rockfish.

누룩 또는 밀기울 첨가식으로 성장시킨 흰쥐의 혈중 Cholesterol 및 간조직 유해산소 대사효소 활성 변동

윤종국(Chong-Guk Yoon),채순님(Soon-Nim Chae),허남응(Nam-Eung Huh),김현수(Hyun-Soo Kim),유대식(Tae-Shick Yu) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.28 No.1

누룩 및 밀기울 첨가식이로 성장한 흰쥐에 있어서 간 조직 중 free radical 대사효소 활성 및 혈청 중 cholesterol치의 변동을 관찰하고자 균주 종류에 따른 몇가지 누룩 및 밀기울을 첨가시킨 사료로 1개월간 사육시킨 후 처치하여 다음과 같은 결과를 얻었다. Asp. terreus 균주함유 누룩섭취군은 성장기간 동안 사료효율이 대조군보다 다소 높은 경향을 보였으며, 밀기울 함유식이군 역시 대조군보다 높게 나타났다. Asp. terreus 균주함유 누룩섭취군에 있어서 간조직 중 GSH 함량은 대조군 보다 유의한 감소를 보였으며 GST 활성은 누룩함유식이로 성장시킨 모든 실험군에서 대조군보다 높게 나타났다. 또한 간조직 중 cytochrome P-450 활성인 aniline hydroxylase 활성 역시 밀기울 섭취군을 제외한 누룩섭취군 모두에서 대체적으로 증가되였다. 간조직 중 superoxide dismutase 활성은 누룩 및 밀기울을 섭취한 모든 실험군과 대조군간에는 별다른 차이를 볼 수 없었으나 xanthine oxidase 활성은 Asp. terreus 균주를 함유치 않은 누룩섭취군에서는 다소 감소되었다. 한편 혈청중 cholesterol치는 Asp. terreus 함유누룩 및 밀기 울 섭취군에서 대체적으로 감소되는 경향을 보였다. 또한 혈청 LDL-cholesterol치는 Asp. sp.(3-6, 12-1), Pen. sp. 균주함유누룩섭취군을 제외한 다른 누룩 및 밀기울 섭취군을 제외한 다른 누룩 및 밀기울 섭취군이 대조군보다 대체적으로 낮게 나타났으며, HDL-cholesterol치는 누룩 및 밀기울 함유식이섭취군에서 대조군보다 대체적으로 높게 나타났다. 이상 실험결과를 종합해 볼 때 실험동물에 Asp. terreus 균주함유 누룩 및 밀기울을 섭취할 경우 유해산소를 무독화시킴과 동시에 생체방어능력을 향상시킬 것으로 생각되며 또한 누룩 섭취시 혈청 cholesterol치를 낮추는 효과를 관찰할 수 있으며 이는 누룩 중 어떤 생리활성물질에 기인된 결과로 생각된다. The effects of nuruk and wheat bran on cholesterol level in serum and activities of free radical metabolizing enzymes were investigated in rats. The rats were fed a diet containing nuruk or wheat bran for one month. Body weight and food intake were measured. Animals were sacrificed after one month. The increased food efficiency ratio throughout whole growth period was observed in the rats fed with either nuruk containing Aspergillus terreus or wheat bran compared with control group on normal diet. In the rats fed with nuruk, hepatic GSH content, glutathione S-transferase activity, hepatic cytochrome P-450 content, and aniline hydroxylase activities were generally increased. In the rats fed with nuruk containing other fungi except Aspergillus terreus, xanthine oxidase activity was decreased. The decreased cholesterol level in serum was observed in rats fed with nuruk prepared from Aspergillus terreus and wheat bran. LDL-cholesterol level was decreased in rats fed with nuruk prepared with other fungi such as Penicillium sp. and Rhizopus sp. But HDL-cholesterol level was increased in all groups fed with nuruk from any fungi and wheat bran. These results suggested that nuruk- or wheat bran-supplemented diet might exert their effect by decreasing cholesterol level in serum and amount of oxygen free radical level.