http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
한효원,박철민 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Development of a sub-50 nm nanopattern of a thin perovskite film is of great challenge with high photo-electronic properties and environmental stability. By employing a nanoimprinting of perovskite self-assembled with a block copolymer, a thin perovskite nanopattern is developed in a large area. Approximately 30 nm nanodomains consisting of the perovskite crystals are templated in the block copolymer and the registry of the perovskite nanodomains is further guided by the topological pattern, giving rise to hierarchically controlled nanostructures of the perovskites. The study reveals that the nanoimprinting promotes the preferential interaction of one of the blocks, resulting in an ultra-thin top skin layer on the nanopattern. The perovskite nanocrystals hierarchically structured in the nanopatterns are highly luminescent with an insulating protecting skin layer, leading to high performance photodetectors with excellent environmental stability.
한효원,조아라,장의순 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.96 No.-
Cytotoxicity of cetyltrimethylammonium bromide (CTAB)-templated silica-coated gold nanorod(CGNR@SiO2) was confirmed to be severe even after the washing process because of the residualCTAB surfactant. Calcination to eliminate the CTAB template induced dissolution of the gold nanorod(GNR) core and caused a blue-shift in the longitudinal surface plasmon resonance (LSPR) mode of theGNR from the near-infrared to visible wavelength. In contrast, the CTAB template was effectively removedfrom CGNR@SiO2 without interference of the plasmonic property of the core GNR by aminefunctionalizedfluorescence dyes such as methylene blue (MB) and rhodamine B (RB). The RB was moreefficient in substituting the residual CTAB through electrostatic interactions than the MB. Moreover, theresulting products exhibited excellent surface-enhanced Raman scattering performance to detect a fewcancer cells. The MB and RB molecules as visible light photosensitizer (PS) were activated by near-infrared (NIR) excitation light through transfer of the NIR-driven plasmonic hot electrons from the coreGNR to the PS molecules. It is noteworthy that the RB-loaded CGNR@SiO2 exhibited excellent cancer cellkilling efficiency via generation of abundant reactive oxygen species (ROS) under NIR laser irradiation,although the concentration of the intracellular GNR was too low to induce the photothermal effect.
Human Embryonic Stem Cell-derived Neuroectodermal Spheres Revealing Neural Precursor Cell Properties
한효원,김장환,강만종,문성주,강용국,구덕본,조이숙 한국발생생물학회 2008 발생과 생식 Vol.12 No.1
만능성 인간 배아줄기세포로부터 확립된 신경줄기세포 또는 신경전구세포는 퇴행성 신경질환 세포치료제로 이용될 수 있는 다양한 종류의 신경세포로 분화 유도될 수 있다. 하지만, 인간 배아줄기세포로부터 신경세포를 생산하기 위한 기술은 아직 많은 장애를 가지고 있다. 인간 배아줄기세포 유래 신경전구세포에서 특징적으로 나타나는 신경관 유사로제트에 대한 이해는 인간 배아줄기세포 신경 분화의 효율을 높이는데 유용한 정보를 제공할 것으로 사료된다. 일반적으로 신경로제트 Neural stem/precursor derived from pluripotent human embryonic stem cells (hESCs) has considerable therapeutic potential due to their ability to generate various neural cells which can be used in cell-replacement therapies for neurodegenerative diseases. However, production of neural cells from hESCs remains technically very difficult. Understanding neural-tube like rosette characteristic neural precursor cells from hESCs may provide useful information to increase the efficiency of hESC neural differentiation. Generally, neural rosettes were derived from differentiating hEBs in attached culture system, however this is time-consuming and complicated. Here, we examined if neural rosettes could be formed in suspension culture system by bypassing attachment requirement. First, we tested whether the size of hESC clumps affected the formation of human embryonic bodies (hEBs) and neural differentiation. We confirmed that hEBs derived from square sized hESC clumps were effectively differentiated into neural lineage than those of the other sizes. To induce the rosette formation, regular size hEBs were derived by incubation of hESC clumps() in EB medium for 1 wk in a suspended condition on low attachment culture dish and further incubated for additional wks in neuroectodermal sphere(NES)-culture medium. We observed the neural tube-like rosette structure from hEBs after days of differentiation. Their identity as a neural precursor cells was assessed by measuring their expressions of neural precursor markers(Vimentin, Nestin, MSI1, MSI2, Prominin-1, Pax6, Sox1, N-cadherin, Otx2, and Tuj1) by RT-PCR and immunofluorescence staining. We also confirmed that neural rosettes could be terminally differentiated into mature neural cell types by additional incubation for wks with NES medium without growth factors. Neuronal(Tuj1, MAP2, GABA) and glial( and GFAP) markers were highly expressed after and 4 wks of incubation, respectively. Expression of oligodendrocyte markers O1 and CNPase was significantly increased after wks of incubation. Our results demonstrate that rosette forming neural precursor cells could be successfully derived from suspension culture system and that will not only help us understand the neural differentiation process of hESCs but also simplify the derivation process of neural precursors from hESCs.
한효원,김소희,박철민 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.1
Metal halide perovskite nanocrystals (PeNCs) are emerging as attractive materials for photonic and optoelectronic applications because of its outstanding photoluminescence (PL) properties. Although tremendous efforts have been made for synthesizing PeNCs, most of them were complicated and time-consuming process. In this work, through simple and time efficient two-step process, highly luminescent PeNCs could be obtained in nano domain of block copolymer (BCP) photonic crystal (PC) films. Structural color of BCP PCs is changed by crosslinking concurrently with crystallization of PeNCs. Combining PL of PeNCs and structural color of BCP PCs, dual-responsive anticounterfeiting devices in which the information can be decrypted by two kinds of stimuli, ultraviolet light and liquids which can swell P2VP domain are achieved.