발표논문초록 : 포스터 발표 ; 온주밀감 미성숙 종자에서 유도된 callus 의 증식과 재분화 조건

한태완,오순자,허인옥,류기중 한국식물생명공학회 ( 구 한국식물조직배양학회 ) 1996 한국식물생명공학회 춘계학술연구발표회 Vol.1996 No.-

Agrobacterium rhizogenes에 의해 유도된 Atropa belladonna 毛狀根의 배양과 Tropane Alkaloid의 成分에 관한 硏究

許仁玉,韓太完 제주대학교 1993 논문집 Vol.37 No.-

. The study was aimed to confirm transformation, tropane alkaloid production and growth rate of hairy roots. Hairy roots were induced by inoculation of stem of sterile plants of Atropa belladonna with Agrobacterium rhizogenes strain 15834, A4, R1000. Agropine and Mannopine were detected in the hairy roots. The growth rate of hairy roots according to medium and sucrose concentration was higher in hairy roots induced by strain 15834 than other strains. especially, the hairy roots induced by strain 15834 produced 113.5g ger 1 in NN 30 liquid medium cultured for 40 days. Besides, the production of tropane alkaloid was 0.086%~0.628% per dry weight according to strain and produced 0.628% in hairy roots induced by R1000.

차나무 callus 의 catechin류 함량분석

오순자,허인옥,한태완 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.2

본 연구는 차나무의 신초를 이용하여 기내에서 여러가지 배양조건에 따른 캘러스의 catechin류 함량을 분석하여 생산성이 높은 배양조건을 찾고자 실시하였다. 신초배양시 캘러스는 0.5mg/L NAA와 1mg/L BA 조합 처리구에서 가장 잘 유도되었다. 이 조건에서 유도된 캘러스를 가지고 thiamin-HCI, PVP, taxifolin과 quercetin을 농도별로 처리하여 4주간 배양하여 생장 및 catechin류 함량을 조사하였다. 캘러스의 생장은 30mg/L thiamin-HCL, 200mg/L PVP, 1 mM taxifolin, 0.01 mM quercetin처리시 대조구에 비해 2~3배의 생장를 보였다. 차나무 잎의 주성분인 catechin류를 분석하고자 신초와 여러조건에서 배양된 캘러스를 가지고 분석한 결과를 보면, 캘러스에서 높은 함량을 보였다. 또한 신초에서의 catechin류는 EGC, EGCG, ECG등은 검출되었으나, EC는 검출되지 않았다. 그러나 배양된 캘러스에서는 4가지 성분이 모두 검출되었다. 각각의 성분들의 최적 배양조건을 보면, EGC는 500mg/L PVP, EC는 100mM quercetin, EGCG는 10 mg/L thiamin-HCI, ECG는 10mM taxifolin처리구에서 가장 높은 함량을 보였다. This study aimed to measure the growth of callus cultured under MS medium with thiamin-HCI as a vitamine. PVP as the antioxidant. taxifolin and quercetin as precursors of catechins and to select the clone producing catechins highly. Callus induction from Thea sinensis young leaf was great in the MS medium with 0.5mg/L NAA and 1 mg/L BA. When components of callus were compared with those of young leaf. Four kinds of catechins(EC, EGC, EGCG, ECG) were detected in cultured callus whereas EC not in young leaf. Catechins were detected most in the medium with 10mg/L thiamin-HCI. Expecially, EC was most as a 6.39mg/g. The content of catechins in PVP treatment was not different each other but was higher in 100 mg/L and 200 mg/L PVP than in control. When the callus was cultured in the medium with taxifolin and quercetin the caffeine content was lower than in the medium with not them. Also, the content of catechins in taxifolin and quercetin treatment was not different each other but it was highest in 10mM taxifolin and 100mM quercetin treatment.

Candidatus phytoplasma malaysianum (16SrXXXII) associated with Elaeocarpus sylvestris decline in South Korea

이건우,한태완,이선근,한상섭 한국산림과학회 2022 Forest Science And Technology Vol.18 No.1

Phytoplasma has been newly detected in Elaeocarpus sylvestris, a tree species that grows on the island of Jeju, South Korea. Various symptoms of infection of E. sylvestris have appeared since 2013, including loss of leaf coloration, such as yellowing, darkening and branch die- back. We analyzed leaves and seeds of E. sylvestris sequences infected by phytoplasma, and found partial 16S rRNA sequences to be 99.5%, and secA genes 98.2% correlated with Malaysian periwinkle virescence group strain ELY-BN1, MaPV, MOP, MYD, and TtWB. Analyzing these sequences and through the virtual restriction fragment length polymorph- ism (virtual-RFLP), we report here the first occurrence of Elaeocarpus decline associated with a phytoplasma strain belonging to 16SXXXII groups, as well as the first detection of phyto- plasma in the seeds of E. sylvestris in South Korea.

한라구절초 잎절편 배양에 의한 식물체 재분화

박영철,김성용,한태완 한국자원식물학회 2004 한국자원식물학회지 Vol.17 No.1

기내에서 삽목 배양중인 한라구절초[( Chrysanthemum zawadskii ssp. coreanum(Nakai) Y.N. Lee)]의 잎으로부터 효과적인 식물체 재생을 위한 NAA와 BA농도 및 배지고형물의 효과를 조사하였다. 0.2% Gelite를 고형물로 하였을 때 한라구절초의 잎 재분화에는 BA농도보다는 NAA 농도가 더 영향을 미쳤으며, NAA 농도가 1 mg/L 이상이면 모든 조직 에서 재분화를 보였다. 신초수는 NAA 단용 처리에서 많은 경향이었으며 NAA 1 mg/L 처리시가 가장 양호하였다. 배지고형물로 agar과 gelite를 가지고 재분화 효과를 조사한 결과, gelite 첨가시 잎절편 당 신초수가 많았으며 agar 첨가시에는 잎절편당 신초수가 적은 반면에 신초의 길이가 길게 나타났다. 한라구절초의 잎절편으로부터 재분화에는 MS에 3% sucrose, 0.8% agar와 NAA 1 mg/L를 첨가하여 25 $^{\circ}C$, 2,000 lux 정도에서 배양하는 것이 효과적이었다. The effect of plant growth regulators and gelling agents for plant regeneration from leaf segment of Chrysanthemum zawadskii ssp. coreanum was investigated. NAA was more effective than BA for plant regeneration. MS medium supplemented with NAA 1 mg/L was the most effective in plant regeneration. The effect of agar and gelite as gelling agent was compared. Agar(0.8%) was more effective than gelite(0.2%) in plant regeneration. Regenerated shoots was successfully increased by shoot grafting in MS medium supplemented with NAA 0.1 mg/L in vitro, and hardened by shoot grafting in artificial soil mix(Peatmoss : Perlite = 1 : 1).

Atropa belladonna 모상근의 생장량과 tropane alkaloid 생산에 관한 연구

허인옥,오순자,한태완,김철수 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.1

Agrobacterium rhizogenes strain R1000에 의해 유도된 Atropa belladonna 모상근을 배지별, NAA, IBA, IAA, 2,4-D를 농도별(0.01-100 mg/l)로 첨가한 NN액체배지에서 생장량과 tropane alkaloid 함량을 HPLC로 이용하여 정량하였다. 모상근 배양 30일째의 각 auxin의 농도별에 따른 생장율은 고농도보다 저농도일때 대체적으로 양호하였으나, 0.1mg/1 2,4-D농도에서는 callus화 하였고 10mg/l 2,4-D, 1mg/l IBA일때 2.96g, 2.36g으로 대조구보다 2.3배의 생장량을 보였다. 농도별에 따른 Tropane alkaloid 함량은 atropine이 대조구 0.141%, 원식물체가 0.059%로 나타났으며, 0.01mg/l NAA와 0.1mg/l IAA에서 배양한 모상근이 각각 1.378%, 1.814%의 생산을 보여 대조구와 원식물체보다 9-30배의 생산을 보였다. 또한 scopolamine 함량은 대조구와 원식물체는 각각 0.109%. 0.050%에 비해 0.01mg/l NAA에서 배양한 모상근에서 0.505%로 대조구 원식물체보다 4-10배의 생산량을 보였다. The study aimed to measure growth rate of Atropa belladonna hairy root cultured under medium, various auxin(NAA, IBA, IAA, 2.4-D) and auxin concentration(0.01-100mg/l) and investigated tropane alkaloid content using HPLC. The growth rate of hairy root cultured for 30 days in MS and NN liquid medium were higher in NN medium as 3.51g than MS medium, growth rate of belladonna hairy root cultured in NN medium added various auxin and auxin concentration was the most effective in low concentration than high concentration, especially, was best in the medium supplemented with 0.01mg/l 2,4-D. Atropine content of hairy root cultured in 0.01mg/l and 0.1mg/l IAA produced 9-30 times than control and normal plant. scopolamine measured each 0.109%, 0.050% in control and normal plant, hairy root cultured in 0.01mg/l NAA produced 4-10 times than control and normal plant.

The bimodal adult activity of Monochamus alternatus (Coleoptera: Cerambycidae) caught in pheromone traps in Jeju can be explained by the competitive attractiveness of dying pine trees

권순화,고명수,박정훈,한태완,김경범,신창훈,김동순 한국곤충학회 2019 Entomological Research Vol.49 No.4

The Japanese pine sawyer, Monochamus alternatus Hope (Coleoptera: Cerambycidae), is known to be the primary vector of pinewood nematode Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle that causes pine wilt disease. Adult activity of M. alternatus caught in a pheromone trap on Jeju Island of Korea showed a bimodal form with the first peak in mid to late June and the second peak in mid to late September. The two peaks were separated between mid and late August, showing a valley. Accumulated degree‐days predicted that the emergence of the second generation adults could be possible just before the second peak. But actually no adults of the second generation occurred in the field development experiments of M. alternatus in 2016 and 2017. Pine trees without oleoresin flow (namely dying trees by the infection of pinewood nematode) were abundant during early July to early August. The bimodal adult activity pattern of M. alternatus could be partially explained by the competitive attractiveness of dying trees against pheromone traps, when we accepted the assumption that dying pine trees attract strongly M. alternatus.

삼보감(Citrus sulcata Hort. et Takahashi) 유실물체에서 유도된 캘러스의 체세포배 형성과 식물체 재분화

박수영,허인옥,부지현,한태완,송관필 濟州大學校 基礎科學硏究所 1998 基礎科學硏究 Vol.11 No.1

삼보감에 있어서 기내 체세표배 발생을 통한 다량증식을 도모하기 위해서 캘러스 증식 및 체세포배 발생, 배로부터 식물체 재분화에 미치는 배지 및 Polyamine, 생장조절제의 효과를 구명하기 위해 실시하였다. 삼보감 과실에서 채집된 종자를 호르몬이 첨가되지 않은 MS배지에서 무균발아시켰다. 발아된 유식물체의 줄기에서 캘러스를 유도하기 위해 NAA와 BA가 첨가된 MT 배지에서 배양하였고 5mg/L 2,4-D와 1mg/L BA가 첨가된 배지에서 계대배양하였다. 캘러스를 증식시키기 위한 배양조건 설정으로 배지별과 Polyamine(Spermidine, Spermine, Putrecine) 농도별(0 - 1mM)에 따른 생장량을 좌하였다.배지별에 따른 생장율은 MT배지에서 0.968g(fr wt)으로 가장 높았고, Polyamine의 영향은 0.01 mM Putrecine 처리구에서 0.78g으로 가장 높게 나왔다. 배발생 캘러스는 0.1mg/L NAA와 0.5mg/L BA가 첨가된 MT배지에서 유도하였으며 유되된 체세포배를 식물체로 재분화시키기 위해서 0.1mg/L NAA와 1mg /L BA가 첨가 된 MT배지에서 배양하였다. This study was performed to investigate the culture condition induction of somatic embryo and plant regeneration in callus induced from Citrus sulcata leaf and stem as a basic research for breeding of new plant. The seeds of Sambokam were germinated in hormone free MS medium under sterile condition. Callus induced form stem and leaf geminated young plant in MT supplemented with NAA plus BA and subcultured in MT supplemented with 5 ㎎/ L 2,4-D and 1 ㎎/L BA. As a investigation of culture condition for callus proliferation, growth rate of callus were investigated in various medium and polyamine concentrations. The effect of medium was most effective in MT medium as 0.968g and polyamine was most effective of in 0.01mM putrecine among various concentration. Formation of embryogenic callus induced from MT medium containing 0.1 ㎎/L and 0.5 ㎎/L BA. The geminated embryos developed to complete plantlet when cultured on MT medium supplemented with 0.1 ㎎/L NAA and 1㎎/L BA.

백합(Lilium longiforum THUNB.)의 원형질체 분리 및 배양조건 설정

허인옥,박수영,부지현,한태완,송관필,김성철 濟州大學校 基礎科學硏究所 1999 基礎科學硏究 Vol.12 No.1

본 연구는 백합육종의 기초자료로 삼고자 longiflorum 계통의 Georgia 엽육조직으로부터 원형질체를 분리, 배양함으로써 재생여부를 확인하고 Georgia와 Macro polo의 원형질체 융합을 시도하였다. 원형질체 분리 적정농도를 알아보기 위해 Onozuka cellulase R-10, Macerozyme R-10, 그리고 Pectolyase를 효소농도별로 처리해 본 결과 삼투조절체는 0.5M mannitol, 효소 농도는 1.0% Cellulase, 1.0% Macerozyme, 0.1% Pectolyase를 처리시 2~3시간대로 분리가 가장 잘 되었다. 원형질체 배양은 NH₄NO₃를 첨가하지 않은 1/2MS수정배지에 1.0mg/L NAA와 1.0mg/L BAP를 혼용처리하였을때가 가장 좋았으며, 액체배지를 이용할 때가 원형질체의 생존율 및 세포분열이 가장 양호하였다. 초기배양 한달 후 세포괴가 형성되었고, 그 후 한천배치로 계대배양하여 재분화를 유도하였다. PEG에 의한 원형질 융합은 그 융합빈도는 높지 않았으나 PEG 50%용액에서 약 20분간 처리시 융합비도가 가장 높게 나타났다. This study was carried out isolation and culture of protoplast from mesophyll tissues of lily(Lilium longiflorum Thumb), and protoplast fusion of Georgia and Macro polo for breeding of lily. The yield of protoplasts was the highest when cells are incubated in the enzyme solution of 0.5M mannitol, 1.0% Onozuka cellulase R-10, 1.0% Macerozyme R-10, and 0.1% Pectolyase for treatment of 2~3 hours. The protoplasts were most effectively cultured in the modified 1/2 MS medium without NH₄NO₃with 1.0mg/L NAA and 1.0mg/LBAP, and also protoplasts survived the best on stabilized liquid layer. Colonies were obtained after 1 month of culture. The calli grew and regenerated shoots by transferring them on the same composition of the solidified medium. The protoplast fusion was performed with the PEG methods. The yield of protoplast fusion was the highest in the 50% PEG concentration treated for 20 minutes.

제주도내 재배용 백합의 바이러스 감염실태와 구근생장에 대한연구

김성철(Seong-Cheol Kim),김몽섭(Mong-Sup Kim),송혜숙(Hye-Suk Song),허인옥(In-Ok Huh),한태완(Tae-Wan Han) 한국원예학회 1996 한국원예학회 학술발표요지 Vol.14 No.2