무궁화 품종간의 상호교잡시 화분관 신장에 관한 연구

한인송 건국대학교 자연과학연구소 1998 建國自然科學硏究誌 Vol.9 No.1

무궁화 자가수분 및 품종간 타가수분시 교배조합별 화분관 신장속도를 알아보기 위하여 15품종간의 수분 3,6,9,12,15 시간 후 화분관 신장율을 조사한 결과는 다음과 같다. 자가수분 Campanha는 수분이 완료되었으나, 대부분은 화주의 40~80% 정도에서 화분관 신장이 멈췄다. 타가수분을 포함한 화합수분의 경우 화분관 신장속도는 수분 9시간 후 12시간 후에 완료되는 빈도가 가장 높았다. 紅花郎의 화분은 교재에 사용되었을 경우 거의 9시간안에 사료되었으며, Campanha의 화분은 대개 12시간만에 신장이 완료되어 화분친으로 사용할 때 교배성공율이 낮은 것으로 사료되었다. 겹꽃의 화분중 새한의 화분은 정상적으로 수분하였으며, 산처녀의 화분은 불임이다. This study was conducted to investigate the elongation speed of pollen tube in pollinated styles of Hibiscus syriacus after self-and cross-pollination between 15 cultivars. Pollen tube growth in styles was observed 3,6,9,12, and 15 hours after self-and cross-pollination. In self-pollination, although the pollen tube was completely reached to the base of style in Campanha, most pollen tube growth were inhibited at the middle of the style. In compatible pollination including cross-pollination, most of pollen tube elongation into style were completed 9 to 12 hours after pollination. Pollen tube elongation of Honghwarang were finished mostly after 9 hours and that of Campanha were finished mostly after 12 hours. In case of double flowers, the pollen of Saehan was completely fertile while the pollen of Sanchonyo was sterile.

배발생캘러스 배양에 의한 칼라 식물체 재분화 체계 확립

한인송,김종보 한국식물생명공학회 2019 JOURNAL OF PLANT BIOTECHNOLOGY Vol.46 No.1

Calla lilies (Zantedeschia spp.) are monocotyledonous ornamental plants which belongs to the Araceae family. After the release of elite calla cultivar, an efficient propagation system is needed for commercial use. Despite the use of conventional propagation methods such as splitting of tubers and rhizomes of calla, rapid and efficient propagation system should be developed. In order to achieve this goal, stem segments contained apical meristems derived from calla lily cultivar (cv. Gag-si) were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of cytokinin and auxin. This was aimed at inducing embryogenic calluses, shoots and multiple shoots. As a result, about 25% of induction rates of yellow embryogenic calluses were observed with MS medium containing both 0.5 mg・L-1 NAA and 1.5 mg・L-1 BA as growth regulators. In the experiments involving the regeneration from embryogenic calluses through shoot formation, MS medium supplemented with 0.5 mg・L-1 IAA and 2.0 mg・L-1 BA showed the highest rates at approximately 85 ~ 90% with regard to the formation of shoots in calla. Moreover, multiple shoots needed for rapid propagation were generated when explants were cultured on MS medium supplemented with 0.5 mg・L-1 IAA and 2.0 mg・L-1 BA with 40% of formation rate. In this study, the combination of auxin and cytokinin showed positive effects on both the induction of embryogenic calluses, the formation of shoots as well as multiple shoots in calla. The regeneration system described here can contribute to the development of breeding programs of calla in the future.

배발생캘러스 배양에 의한 칼라 식물체 재분화 체계 확립

한인송,김종보,Han, In-Song,Kim, Jong Bo 한국식물생명공학회 2019 식물생명공학회지 Vol.46 No.1

칼라는 천남성과에 속하는 단자엽식물이다. 칼라 신품종이 출시되고 나서 상업적 이용을 위해서는 효율적인 번식체계가 필요하다. 기존 칼라에서 분주나 분구등으로 번식이 이루어졌지만 효율이 낮아서 빠르고 효율적인 번식체계의 개발이 필요한 실정이다. 이러한 목적을 달성하기 위해서 칼라 'Gag-si' 품종의 경정조직이 포함된 줄기절편체들을 다양한 농도의 오옥신과 사이토키닌이 첨가된 MS배지에 배양하여 배발생캘러스, 신초 및 다신초를 유도하고자 하였다. 그 결과, MS배지에 $0.5mg{\cdot}L^{-1}\;NAA$와 $1.5mg{\cdot}L^{-1}\;BA$가 첨가된 배지에서 약 25%의 배발생캘러스 형성율을 보여주었다. 재분화실험에서는 $0.5mg{\cdot}L^{-1}\;IAA$와 $2.0mg{\cdot}L^{-1}\;BA$가 첨가된 MS배지에서 85 ~ 90%의 신초 형성율을 보여주었으며, 다신초의 경우는 같은 농도에서 약 40%의 형성율을 나타내었다. 본 연구에서 오옥신과 사이토키닌의 호르몬 조합이 배발생캘러스, 신초 및 다신초 유도에 있어서 긍정적인 효과를 나타내었다. 본 연구에 기술된 재분화 체계는 향후 칼라 육종프로그램 발전에 기여할 것이라 판단된다. Calla lilies (Zantedeschia spp.) are monocotyledonous ornamental plants which belongs to the Araceae family. After the release of elite calla cultivar, an efficient propagation system is needed for commercial use. Despite the use of conventional propagation methods such as splitting of tubers and rhizomes of calla, rapid and efficient propagation system should be developed. In order to achieve this goal, stem segments contained apical meristems derived from calla lily cultivar (cv. Gag-si) were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of cytokinin and auxin. This was aimed at inducing embryogenic calluses, shoots and multiple shoots. As a result, about 25% of induction rates of yellow embryogenic calluses were observed with MS medium containing both $0.5mg{\cdot}L^{-1}\;NAA$ and $1.5mg{\cdot}L^{-1}\;BA$ as growth regulators. In the experiments involving the regeneration from embryogenic calluses through shoot formation, MS medium supplemented with $0.5mg{\cdot}L^{-1}\;IAA$ and $2.0mg{\cdot}L^{-1}\;BA$ showed the highest rates at approximately 85 ~ 90% with regard to the formation of shoots in calla. Moreover, multiple shoots needed for rapid propagation were generated when explants were cultured on MS medium supplemented with $0.5mg{\cdot}L^{-1}\;IAA$ and $2.0mg{\cdot}L^{-1}\;BA$ with 40% of formation rate. In this study, the combination of auxin and cytokinin showed positive effects on both the induction of embryogenic calluses, the formation of shoots as well as multiple shoots in calla. The regeneration system described here can contribute to the development of breeding programs of calla in the future.

自生草花類 種子의 光과 溫度에 따른 發芽特性 : Native Flower Seeds According to Light and Temperature

김재건,한인송 건국대학교 자연과학연구소 1999 建國自然科學硏究誌 Vol.10 No.1

This study was carried out to investigate the optimum condition of temperature and light for germination in Korean-native flower seeds. The typical light germinator were Androsace umbellata, Gentiana squarrosa, and Lythrum anceps. They germinated at relatively high temperature. Dark germinator was Clematis mandshurica, and it germinated at relatively low temperature. Seeds of Chrysanthemum boreale, Senecio mtegrifoltus var. spathulatus, Dianthus stnensis, Meoilotus suavelens, and A ristolochia contorta germinated at both light and dark condition Most seeds as Orostachys japonicus, Leonurus inacranthus, A stilbe chinensis var. davidii, Campanula glomerata var. dahurica, Lysimachia barystachys, Veronica linariaefolia, Aster yomena, Valeriana fauriei, and Clinopodium chinense var. parvuflorum showed higher germination rate at light condition than at dark condition. The optimum temperature of Astilbe chtnensis var. davidii was 15℃, but at of Parnassia palustris was 25℃. 0ther seeds germinated at wide range of temperature. We classified the germination type of Korean-native flower seeds into 5 types according to maximum germination rate at light and dark condition. Type I seeds were light germinator and non-germinating at dark. It contains Androsace umbellata, Gentiana squarrosa, and Lythrum anceps. Type Ⅱ seeds were light germinator and dark germinating by 50% of maximum light-germinating rate. It contains Parnassia palustris and Clinopodium chinenes var. parviflorum. Type Ⅲ seeds were light germinator and dark germinating by 51~9o% of maximum light-germinating fate. It contains Orostachys japonicu, Leonurus macranthus, A stilbe chinensis var. davidii, Campanula glomerata var. dahurica Lystmachia barystachys, Veronica linearifolia, A ster cillosus, and Valeriana fauriel. Type Ⅳ was well germinated at both light and dark condition, so it was impossible to distinguish. It contains Chrysanthemum boreale, Senecio integrifolius var. spathulatus, Dianthus sinensis, Meoilotus suaveolens, and Aristolochia contoria Type Ⅴ seeds were dark germinator and light germinating by 51~90% of maximum dark-germinating rate. It contains Clematis mandshurica We had no seed showing type Ⅵ( contrary to type Ⅲ) and type Ⅶ (contrary to type Ⅰ). But we could estimate the presence of type Ⅵ which would be dark germinating seeds and also light germinating by 50% of maximum dark-germinating rate, and type Ⅶ which would be dark germinating seeds and non-germinating under light.

무궁화의 自家不和合群, 自家和合群 및 不稔群間의 交雜時 花柱內에서의 花粉管 伸長

韓仁松(Han In-Song) 한국원예학회 1995 한국원예학회 학술발표요지 Vol.13 No.1

몇가지 생장억제제 처리가 층층이꽃의 개화와 생육 및 종자발아에 미치는 영향

한인송(In-Song Han),김재건(Jae-Gun Kim) 한국원예학회 1999 원예과학기술지 Vol.17 No.3

6. 무궁화 自家不和合性에 関한 研究

韓仁松(Han In Song),廉道義(Yeam Do Yi) 한국원예학회 1985 한국원예학회 학술발표요지 Vol.3 No.1

7. 무궁화 自家不和合性에 関한 研究

韓仁松(Han In Song),廉道義(Yeam Do Yi),車道仁(Cha Do In) 한국원예학회 1985 한국원예학회 학술발표요지 Vol.3 No.1

5. 무궁화 自家不和合性에 関한 研究

韓仁松(Han In Song),廉道義(Yeam Do Yi) 한국원예학회 1985 한국원예학회 학술발표요지 Vol.3 No.1

冬季 비닐터널栽培가 튤립 球根生産에 미치는 影響

한인송,허건양 건국대학교 자연과학연구소 1998 建國自然科學硏究誌 Vol.9 No.2

튤립 구근생산시 정식 후 비닐터널재배에 의한 구근증식 촉진효과를 알아보기 위하여 11월 22일 정식 후 동계 피복과 춘계 피복실험을 수행한 결과는 다음과 같다. 생육은 노지재배구나 춘계 피복구보다 동계 피복구가 출아일과 개화일이 현저히 빨랐으며 출아율과 개화율도 현저히 높았다. 등급별 생산구근수도 노지재배구나 춘계 피복구보다 동계 피복구가 고도로 유의하게 많았으며 특히 2~4등구의 대구가 많이 생산되었다. 등급별 생산구근 중량도 노지재배구나 춘계 피복구보다 동계 피복구가 고도로 유의하게 많았으며 특히 2~4등구의 대구와 7등구 이하의 소구 합계에서도 더 많이 생산되었다. 우리나라의 튤립의 구근생산 재배시 동계 비닐 터널재배가 필요하다고 사료되었다. This study was conducted to investigate the effect of culture under row cover with plastic film on the increase of bulb production in Tulipa gesneriana L. 'Red Matador.' Tulip bulbs of 7~8cm dia. size were planted at November 22, covered with PE film at December 5 and March 1, and harvested June 2. Both days to sprouting and flowering of culture under row cover during winter were significantly promoted than field culture without cover. The rate of sprouting and the rate of flowering under winter tunnel were also significantly higher than on field. The number and weight increase of bulbs produced under tunnel were highly significant. Especially, bigger bulbs as 9~12cm size were produced under winter tunnel culture. It was assummed that winter tunnel culture of tulip bulb production was essential to prolong the bulb proliferation period in Korea.