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PMA 처리한 인간 불멸화 상피세포에서 청색 및 적색 광 조사에 따른 활성산소 제거에 대한 비교 연구
한세우,고영종,정진안,김지은,김인애,임원봉,임회순,박지일,김미숙,김서연,김옥준,최홍란 대한구강악안면병리학회 2009 대한구강악안면병리학회지 Vol.33 No.1
Intracellular reactive oxygen species(ROS) produced in a various pathologic state was known to intermediate many cellular response such as inflammation. Recently, low level light irradiation by HeNe laser used in many clinical field could improve inflammatory state by scavenging intracellular ROS through photo-detachment/dissociation process. The purpose of this study is to investigate the differential effects of blue and red light irradiation on ROS scavenging effects. Immortalized human oral keratinocyte HaCat cells were used. Phorbol 12-myristate 13-acetate(PMA) was treated for inflammation. Red(635nm) and blue(470nm) light irradiation was carried out. To asses the intracellular ROS by light irradiation, confocal microscopic and flow cytometric assay with DCF fluorescence for total ROS and ESR spectrometry of DMPO-O2- for superoxide anion were caried out. And microarray was performed for mRNA expression level. Released intracellular total ROS in PMA treated HaCat cell lines was dissociated efficiently by red light irradiation, while blue light irradiation did not. Rather, blue light irradiation increased ROS formation. For superoxide anion generated the first synthetic form of ROS, red light irradiation reduced its amount but blue light irradiation did not. In the mRNA expression in line with cyclooxygenase(COX) pathway, prostagrandin endoperoxide synthase 1(PTGS 1), prostagrandin endoperoxide synthase 2(PTGS 2) and phospholipase A2(PLA2) were increased by both light irradiation and they were decreased as time flows. And genes associated with ROS releasing, mRNA expressions of tumor necrosis factor receptor (TNFR) and interleukin 1beta(IL1B) were increased by 1 hour red light irradiation but did not by blue light irradiation. As a result, red and blue light irradiation showed different response in affecting the level of ROS. These findings indicate that red light rather than blue light is more useful for anti-inflammation in clinical field.
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한세우,김명래,김재화,배종고,Han, Sei-Woo,Kim, Myung-Rae,Kim, Jae-Hwa,Bae, Jong-Go 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.1
This is to evaluate the postoperative cephalometric changes of the chin advanced by the genioplasty. Thirty-eight patients who underwent advancement genioplasties at Ewha Womans University Mokdong Hospital between January of 2001 and February of 2003. They were followed up for at least 12 months with lateral cephalographs. The remodelling changes of the horizontal linear measurement between the Pogonion and Perpendicular line to FH plane were measured and analysed by Independent sample tests. The chin advancement resulted in $1.3{\pm}0.3mm$ resorption (28.2% of advancement) after 6 months, but in $1.4{\pm}0.2mm$ after 1 year. If the chin was advanced less than 3 mm, remodelling followed by $1.2{\pm}0.2mm$ reduction, but $1.3{\pm}0.3mm$ reduction followed after chin advanced over 4 mm. There were no significant differences in the amount of bone resorption by the gender, number of genial cut-steps and acompanied osteotomies.
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한세우,김명래,김재화,배종고 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.1
Remodelling Changes of the Chin Advanced by Genioplasty
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PMA 처리한 인간 불멸화 상피세포에서 청색 및 적색 광 조사에 따른 활성산소 제거에 대한 비교 연구
한세우,고영종,정진안,김지은,김인애,임원봉,임회순,박지일,김미숙,김서연,김옥준,최홍란 대한구강악안면병리학회 2009 대한구강악안면병리학회지 Vol.33 No.1
Intracellular reactive oxygen species(ROS) produced in a various pathologic state was known to intermediate many cellular response such as inflammation. Recently, low level light irradiation by HeNe laser used in many clinical field could improve inflammatory state by scavenging intracellular ROS through photo-detachment/dissociation process. The purpose of this study is to investigate the differential effects of blue and red light irradiation on ROS scavenging effects. Immortalized human oral keratinocyte HaCat cells were used. Phorbol 12-myristate 13-acetate(PMA) was treated for inflammation. Red(635nm) and blue(470nm) light irradiation was carried out. To asses the intracellular ROS by light irradiation, confocal microscopic and flow cytometric assay with DCF fluorescence for total ROS and ESR spectrometry of DMPO-O2 - for superoxide anion were caried out. And microarray was performed for mRNA expression level. Released intracellular total ROS in PMA treated HaCat cell lines was dissociated efficiently by red light irradiation, while blue light irradiation did not. Rather, blue light irradiation increased ROS formation. For superoxide anion generated the first synthetic form of ROS, red light irradiation reduced its amount but blue light irradiation did not. In the mRNA expression in line with cyclooxygenase(COX) pathway, prostagrandin endoperoxide synthase 1(PTGS 1), prostagrandin endoperoxide synthase 2(PTGS 2) and phospholipase A2(PLA2) were increased by both light irradiation and they were decreased as time flows. And genes associated with ROS releasing, mRNA expressions of tumor necrosis factor receptor (TNFR) and interleukin 1beta(IL1B) were increased by 1 hour red light irradiation but did not by blue light irradiation. As a result, red and blue light irradiation showed different response in affecting the level of ROS. These findings indicate that red light rather than blue light is more useful for anti-inflammation in clinical field
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악교정 수술 환자에서 수술부 술전소독에 사용되는 소독제의 항균성에 대한 임상적인 비교 : POVIDONE-IODINE AND CHLORHEXIDINE
엄기훈,한세우,유준영,양병은,김용관 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2
For prevention of bacteremia and surgical field contamination, various concentration are used in preoperative skin preparation. Of these, povidone(PVP)-iodine has been known as one having believable effect. But, application of PVP-Ⅰon pral and maxillofacial field has some problems-skin discoloration, hypersensitivity, corneal irritation,etc. Therefore chlorhexidine has been tried to replace PVP-Ⅰ. More recent studies of antibacterial effect of chlorhexidine and PVP-Ⅰ has been achieved on the other field. The purpose of this study was to compare the antibactreial effect of two kinds of skin disinfectants used in oral and maxillofacial surgical field.-chlorhexidine and PVP-Ⅰ. We studied the 40 patients who have taken operation of orthognathic surgery in Dept. of oral & Maxillofacial Surgery, Kangnam General Hospital Public Corp..Results of bacterial culture taken from facial skin and intraoral mucosa after application 10% PVP-Ⅰ and 0.5% chlorhexidine were compared.
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