흰쥐에 있어서 피부노출 차단이 피부조직의 Xanthine Oxidase 활성에 미치는 영향

한선일,전태원,윤종국 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.1

횐쥐에 있어서 피부의 공기차단이 피부조직의 xanthine oxidate (XO) 활성에 미치는 영향을 검토하고자 직경 46 mm, 높이 10 mm인 petri dish-shaped glass chamber에 순간접착제 (α-cyanoacrylate)를 이용하여 실험동물의 등 부위 피부조직에 부착시켜 10일간 공기의 접촉을 차단하였다. 공기 접촉 차단 5일째에 피부의 땀 축적량은 약 400 mg 정 도이었으나, 10일째 에는 약 25 mg으로 감소하였다. 5일간 피부차단 시 피부조직 중 XO활성은 대조군에 비해 증가하였으며,그 증가율은 chamber 내의 땀 축적량과 관련하여 10일간 피부차단군 보다 높게 나타났다. 5일간 피부차단 시 XO의 기질 농도 변화에 따른 반응 속도를 관찰한 결과, 대조군에 비해 V-max치가 높게 나타났다. 이상 실험 결과를 보아, 피부의 공기접촉 차단으로 피부조직 중 XO효소 활성이 증가된 것은 이 효소 단백 합성 유도에 기인되며, 이는 피부조직에서 oxygen free radical의 생성을 유도하여 외부 환경에 대한 방어장벽작용에 관여할 것으로 생각된다. To investigate an impact of skin occlusion on the dermal xanthine oxidase (XO) activity, the dorsal part in rats was covered with closed petri dish-shaped chamber, 46 mm in diameter and 10mm in height, which was made of glass. The crack between top of chamber and skin was sealed by an adhesive agent. After 5 days, the quantity of sweat accumulation was about 400 mg, whereas after 10 days that was decreased about to 25 mg. The 5 days skin occlusion showed the more increased activity of dermal XO compared with the control, and the increased ratio of enzyme activity to the control was higher than that of 10 days skin occlusion, with the increase being associated with sweat accumulation in chamber. Furthermore, the Vmax of dermal XO in 5 days skin occlusion was higher than that in the control. In conclusion, it may be hypothesized that the XO system may play an role for defence mechanism in dermal tissue.

흰쥐에 있어서 피부노출 차단이 피부조직의 Xanthine Oxidase 활성에 미치는 영향

한선일(Sun-Il Han),전태원(Tae-Won Jeon),윤종국(Chong-Guk Yoon) 대한의생명과학회 2000 Biomedical Science Letters Vol.6 No.1

흰쥐에 있어서 피부의 공기차단이 피부조직의 xanthine oxidase (XO) 활성에 미치는 영향을 검토하고자 직경 46 ㎜, 높이 10 ㎜인 petri dish-shaped glass chamber에 순간접착제 (α­cyanoacrylate)를 이용하여 실험동물의 등 부위 피부조직에 부착시켜 10일간 공기의 접촉을 차단하였다. 공기접촉 차단 5일째에 피부의 땀 축적량은 약 400 ㎎ 정도이었으나, 10일째에는 약 25 ㎎으로 감소하였다. 5일간 피부차단 시 피부조직 중 XO 활성은 대조군에 비해 증가하였으며, 그 증가율은 chamber 내의 땀 축적량과 관련하여 10일간 피부차단군 보다 높게 나타났다. 5일간 피부차단 시 XO의 기질 농도 변화에 따른 반응 속도를 관찰한 결과, 대조군에 비해 Vmax 치가 높게 나타났다. 이상 실험 결과를 보아, 피부의 공기접촉 차단으로 피부조직 중 XO 효소 활성이 증가된 것은 이 효소 단백 합성 유도에 기인되며, 이는 피부조직에서 oxygen free radical의 생성을 유도하여 외부 환경에 대한 방어장벽작용에 관여할 것으로 생각된다. To investigate an impact of skin occlusion on the dermal xanthine oxidase (XO) activity, the dorsal part in rats was covered with closed petri dish-shaped chamber, 46 ㎜ in diameter and 10 ㎜ in height, which was made of glass. The crack between top of chamber and skin was sealed by an adhesive agent. After 5 days, the quantity of sweat accumulation was about 400 ㎎, whereas after 10 days that was decreased about to 25 ㎎. The 5 days skin occlusion showed the more increased activity of dermal XO compared with the control, and the increased ratio of enzyme activity to the control was higher than that of 10 days skin occlusion, with the increase being associated with sweat accumulation in chamber. Furthermore, the Vmax of dermal XO in 5 days skin occlusion was higher than that in the control. In conclusion, it may be hypothesized that the XO system may play an role for defence mechanism in dermal tissue.

연령이 다른 흰쥐에 Bromobenzene 투여가 간 손상에 미치는 영향

한선일(Sun-Il Han),윤형원(Hyung-Won Yoon),윤종국(Chong-Guk Yoon) 대한의생명과학회 1999 Biomedical Science Letters Vol.5 No.2

연령이 다른 흰쥐에 있어서 bromobenzene에 의한 간 손상의 정도 차이와 이의 기전을 알아보기 위하여 5주령 및 10주령 흰쥐에 bromobenzene을 체중 ㎏ 당 400 ㎎을 격일로 5회 전처치함과 동시에 bromobenzene 전처치 및 대조군에 bromobenzene을 다시 투여한 뒤 처치하여 다음과 같은 결과를 얻었다. Bromobenzene 전처치로 인한 간 무게, 혈청 alanine aminotransferase, xanthine oxidase 활성 및 간 조직 중 과산화지질의 함량 증가율과 간 세포질의 단백질 감소율은 10주령군이 5주령군 보다 높았다. 이 결과는 10주령이 5주령 보다 bromobenzene 투여로 인한 간 손상이 심하게 나타남을 시사해 주고 있다. 한편, 간 조직 중 aniline hydroxylase 활성은 10주령에 있어서 bromobenzene 전처치군 및 대조군 모두 5주령 보다 높게 나타나는 반면 glutathione S-transferase 활성은 10주령이 5주령 보다 낮게 나타났다. 또한 이들 실험동물에 bromobenzene을 재 투여한 다음 경시별로 간 조직 중 glutathione 함량을 측정한 바 10주령이 5주령 보다 glutathione 감소율이 낮게 나타났다. 이상 실험결과를 종합해 볼 때 bromobenzene 투여시 10주령이 5주령 실험군 보다 간 손상이 심하게 나타났으며, 이는 생체내의 bromobenzene 처리능력이 5주령 보다 10주령이 저하되기 때문에 나타난 결과라고 사료된다. To evaluate an effect of growth periods on the bromobenzene-induced liver damage, bromobenzene was administrated to 5-week-old rats and 10-week-old rats pretreated with bromobenzene 5 times every other day for 10 days and then the animals were sacrificed. The results were obtained as follows; The increasing rate of serum levels of alanine aminotransferase, xanthine oxidase activity, hepatic lipid peroxide contents, liver weight per body weight (%) and decreasing rate of hepatic contents of protein to each control group were higher in 10-week-old rats than 5-week-old rats by the pretreatment of bromobenzene. According to the above results, 10-week-old rats indicated more severe liver injury than 5-week-old those in case of bromobenzene pretreatment. On the other hand, hepatic aniline hydroxylase activity was more increased in 10-week-old rats than 5-week-old rats both in control and bromobnezene pretreated rats where as the reverse in hepatic glutathione S-transferase. In case of hepatic GSH determination at the intervals of 2, 4, 8, 24 hours throughout 24 hr after administration of single dose of bromobenzene to 5-week-old and 10-week-old rats both in control and bromobnezene pretreated, the rate of GSH utilization was lower in 10-week-old rats than 5-week-old rats. In conclusion, from the above experimental, it is deduce that the 10-week-old rats showed more severe liver injury than 5-week-old rats by the bromobenzene treatment because the disposal ability of bromobenzene in liver was lower in 10-week-old rats than 5-week-old rats.

연령이 다른 흰쥐에 Bromobenzene 투여가 간 손상에 미치는 영향

윤종국,한선일,윤형원 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCINE 1999 Journal of biomedical laboratory sciences Vol.5 No.2

연령이 다른 흰쥐에 있어서 bromobenzene에 의한 간 손상의 정도 차이와 이의 기전을 알아보기 위하여 5주령 및 10주령 흰쥐에 bromobenzene을 체중 kg당 400mg을 격일로 5회 전처치함과 동시에 bromobenzene 전처치 및 대조군에 bromobenzene을 다시 투여한 뒤 처치하여 다음과 같은 결과를 얻었다. Bromobenzene 전처치로 인한 간 무게, 혈청 alanine aminotransferase, xanthine oxidase 활성 및 간 조직중 과산화지질의 함량 증가율과 간 세포질의 단백질 감소율은 10주령군이 5주령군 보다 높았다. 이 결과는 10주령이 5주령 보다 bromobenzene 투여로 인한 간 손상이 심하게 나타남을 시사해 주고 있다. 한편, 간 조직 중 aniline hydroxylase 활성은 10주령에 있어서 bromobenzene 전처치군 및 대조군 모두 5주령 보다 높게 나타나는 반면 glutathione S-transferase 활성은 10주령이 5주령 보다 낮게 나타났다. 또한 이들 실험동물에 bromobenzene을 재 투여한 다음 경시별로 간 조직 중 glutathione 함량을 측정한 바 10주령이 5주령 보다 glutathione 감소율이 낮게 나타났다. 이상 실험결과를 종합해 볼 때 bromobenzene 투여시 10주령이 5주령 실험군 보다 간 손상이 심하게 나타났으며, 이는 생체내의 bromobenzene처리능력이 5주령 보다 10주령이 저하되기 때문에 나타난 결과라고 사료된다. To evaluate an effect of growth periods on the bromobenzene-induced liver damage, bromobenzene was administrated to 5-week-old rats and 10-week-old rats pretreated with bromobenzene 5 times every other day for 10 days and then the animals were sacrificed. The results were obtained as follows; The increasing rate of serum levels of alanine aminotransferase, xanthine oxidase activity, hepatic lipid peroxide contents, liver weight per body weight (%) and decreasing rate of hepatic contents of protein to each control group were higher in 10-week-old rats than 5-week-old rats by the pretreatment of bromobenzene. According to the above results, 10-week-old rats indicated more severe liver injury than 5-week-old those in case of bromobenzene pretreatment. On the other hand, hepatic aniline hydroxylase activity was more increased in 10-week-old rats than 5-week-old rats both in control and bromobnezene pretreated rats where as the reverse in hepatic glutathione S-transferase. In case of hepatic GSH determination at the intervals of 2, 4, 8, 24 hours throughout 24 hr after administration of single dose of bromobenzene to 5-week-old and 10-week-old rats both in control and bromobnezene pretreated, the rate of GSH utilization was lower in 10-week-old rats than 5-week-old rats. In conclusion, from the above experimental, it is deduce that the 10-week-old rats showed more severe liver injury than 5-week-old rats by the bromobenzene treatment because the disposal ability of bromobenzene in liver was lower in 10-week-old rats than 5-week-old rats.

MgO Tunnel Barrier와 비정질금속 에서의 계면효과 분석

정구열,김종현,한선일,김동석,김홍석,신경호,이긍원 한국자기학회 2009 한국자기학회 학술연구발표회 논문개요집 Vol.2009 No.12

흰쥐의 전립선에서 에탄올 투여에 의한 Apoptosis 및 항산화효소계의 변동

박남조,전태원,한선일,김지연,윤형원,윤종국,이상일,신중규 한국식품과학회 1999 한국식품과학회 학술대회 Vol.1999 No.1

모발생장기 유도 C3H생쥐에 있어서 미녹시딜과 생약추출 혼합 조성물의 모발 재성장 유도 효능

이계호(Kye Ho Lee),한선일(Sun Il Han),박길홍(Gil Hong Park),권영이(Young Ee Kwon) 대한약학회 2003 약학회지 Vol.47 No.1

The hair cycle consists of three phases, growth (anagen), involution (catagen) and quiescence (telogen) phases. In order to evaluate hair re-growth effect of herbal extracts mixture containing the 70% ethanol extracts of Polygoni Mulitiflori Radix, Mori Cortex Radix, Gingo Biloba Folium and Pine bud, we have examined the induction of the anagen phase and elongation of the anagen period using C3H mice. MorPhological examination was done by Hattori and Ogawa's method. Enzyme activites of γ-glutamyl transpeptidase (γ-GT) and alkaline phosphatase(ALP) was detected by Bessey-Lovry-Brock's method. Enzyme activity as a biochemical marker of cycle was investigated in the third hair cycle period of C3H mice after depilation. 3% Milnoxldll treated group and herbal extract mixture treated group were shown 3 days earlier initiation of anagen than control group. In cycling mousse skin, γ-GT activity is pronounced during anagen and greatly diminished during telogen. Herual extract mixture has shown promising hair re-growth efect on hair follicula cycles of C3H mice.

The Magnetic Properties of Perpendicular FePt Thick Films and the Written Patterns for a T-ray Source Undulator

최성원,장평우,이병철,정희준,이긍원,한선일 한국물리학회 2010 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.57 No.3

The feasibility of a micro-undulator using several 2.6 µm thick FePt films and perpendicular recording techniques was confirmed in a previous study. However, samples with this thickness can not be recorded magnetically, because of the large coercive force. A writable low coercive force with a high enough stray field of 3 kOe for a facing undulator was achieved with a 20 µm thick FePt film. The coercivity of the FePt film was reduced by increasing the film’s thickness and by changing the Fe and the Pt compositions. The written magnetization was confirmed with Bitter patterns and Hall effect mesurements. The stray magnetic field measured with a Hall sensor after perpendicular magnetic recording was confirmed from simulations and matched the exprimental value very well. This thick perpendicular magnetic film with magnetic recording can be applied to spintronics devices as well as micro magnet for a THz source.