좀목형 (Vitex negundo var. insica) 신초의 기내증식에 미치는 cytokinin, GA 및 IBA의 영향

한무석,문흥규,박소영,김용욱,손석규 경상대학교 농업생명과학연구원 2011 농업생명과학연구 Vol.45 No.3

향료 및 약용 수종으로 가치 있는 좀목형 (Vitex negundo var. insica)의 기내증식 기술을 개발하고자 3년생 나무의 당년생 신초지로부터 채취한 신초의 기내 증식 및 발근에 미치는 생장조절제의 효과를 구명하였다. 다경줄기 유도는 0.5-2.0 mg/L BA가 첨가된 WPM 배지에서 효과적이었고, 가장 많은 줄기 수(7.9개/절편)는 1.0 mg/L BA 농도에서 얻었다. 줄기 생장은 WPM 기본배지에서 1-2개의 우세 줄기로 자라는 특징이 있었고 길이는 3.4 cm 정도이었다. BA 2.0 + GA 0.5 mg/L 혼용처리는 증식과 더불어 생장을 촉진하는 효과를 보였다. 줄기 증식 시 처리된 생장조절제는 차후의 발근에 영향을 미치는 것으로 나타났다. 전반적으로 BA 처리로 유도된 줄기는 차후 발근도 잘되었으나 고농도 BA 처리 (4.0 mg/L)는 발근을 억제하였다. 저농도의 TDZ 처리도 BA 처리와 유사한 경향을 보였으나 0.5 mg/L 농도로 유도된 줄기는 발근이 현저히 억제되었고 그 이상의 농도에서 유도된 줄기는 전혀 발근되지 못했다. 증식 시 BA에 IBA를 혼용처리하면 증식은 물론 차후의 발근에 가장 좋은 것으로 나타났다. 이상의 결과는 약용가치가 뛰어난 좀목형의 기내배양을 통한 효율적인 증식 가능성을 보여주었다. To develop an efficient micropropagation technique for Vitex negundo var. insica, which is known as aromatic and medicinal tree, the effects of various plant growth regulators (PGRs) on in vitro shoot proliferation and rooting were evaluated using the newly-developed shoots of a 3-year-old tree. Multiple shoot induction was achieved effectively on WPM (woody plant medium) supplemented with 0.5-2.0 mg/L BA, and the highest shoot number (7.9/explant) was obtained at the concentration of 1.0 mg/L BA. Typically 1 or 2 superior shoots (about 3.4 cm) were induced on hormone-free WPM. Combined treatment of BA 2.0 + GA 0.5 mg/L appeared to effective on shoot proliferation and rooting. Plant growth regulators added in shoot proliferation medium had strong impact on subsequent rooting as well. Overall, shoots induced by BA treatment resulted in high rooting rates while the effect was reduced gradually by ascending BA levels. TDZ of low concentration also revealed a similar tendency as BA, but the rooting ability was strongly inhibited at the concentration of 0.5 mg/L, and rooting was never observed at the concentrations higher than 0.5 mg/L. Combined treatment of BA and IBA had positive influence in both shoot proliferation and rooting. These results suggest that Vitex negundo var. insica could be effectively micropropagated via axillary bud cultures.

멸종위기식물 피뿌리풀의 기내증식

한무석,문흥규,강영제,김원우,강병서,변광옥 한국식물생명공학회 2004 JOURNAL OF PLANT BIOTECHNOLOGY Vol.31 No.1

In order to develop an efficient micropropagation technique for an endangered species, Stellera roseaN., stem node cultures were conducted on MS medium supplemented with cytokinins. Generally, BA was betterthan zeatin on shoot proliferation from stem nodes, whereas zeatin showed more effective on shoot elongation. Invitrorooting of shoots was achieved by application of an auxin pre-culturing method. Overall rooting rate wasrelatively low and differed depending on the culture period. Pre-culturing of shoots for 15 days at 1.0 mg/L IBArevealed a slightly better rooting efficiency reaching 30% rooting rate than NAA. Root induction rate by NAA alsovaried with concentration of NAA and culture periods. Total 51% of the rooted plantlets survived on artificial soilmixture and grew normally without any distinct morphological variation. The results suggest that the endangeredStelleraplants are propagated via in vitro culture system, but still need to more study for the improvement of rootingand acclimatization of the plantlets in soil. 멸종위기에 직면한 피뿌리풀 (Stellera rosea N.)의 기내증식법 을 개발하고자 액아 마디를 MS 배지에 BAP와 zeatin을 처리하여 다경 (multiple shoot)를 유도하고 기내발근에 미치는 IBA 및 NAA 처리 효과를 조사하였다. 액아 마디로부터 다경 유도는 BA가 현저히 양호한 반면 줄기의 생장은 zeatin이 BA보다 효과적이었다. 증식된 줄기로부터 기내 발근은 오옥 신 처리로 가능하였으나 발근율은 대체로 저조하였고 오옥신 의 전처리 기간에 따라 차이를 보였다. IBA가 NAA보다 다소 좋은 발근효과를 보였고 1.0 mg/L 농도로 15일간 배양시 30% 까지 발근되었다. NAA 역시 처리농도 및 처리기간에 따라 발 근율에 차이를 보였다. 발근묘는 인공토양에서 51%가 활착되 어 정상생장이 가능하였다. 이상의 결과는 발근 및 순화조건 을 좀더 개선하면 피뿌리풀의 효율적인 기내번식이 가능함을 시사해준다.

희귀식물 제주황기의 미세번식

한무석,노설아,곽명철,문흥규 한국식물생명공학회 2014 식물생명공학회지 Vol.41 No.2

In order to develop an efficient in vitro micropropagationtechnique for a rare plant species, Astragalusmembranaceus Bunge var. alpinus N., shoot proliferationand in vitro or in vivo rootings were conducted andhyperhydrated leaf generated from cultures was histologicallyobserved. During shoot induction, no distinct effect onmultiple shoot induction was found between BA and kinetintreatment. BA enhanced the number of internodes, whereaskinetin stimulated shoot elongation. Hyperhydrated leafcomposed of bigger cells and retarded palisade parenchymaand showed irregular cell arrangement compared to normalleaf. Especially starch content in hyperhydrated leaf wassignificantly reduced. The best rooting rate was achieved byB5 medium among three different medium (B5, MS andWPM) and 0.1mg/L IBA treatment induced the highestrooting ratio (80%). No statistical difference was induced byexplant types (apical bud or axillary bud) in terms of rootingratio. In vivo cutting induced rooting rate up to 65% by 0.5%IBA/Talc powder treatment. Although in vivo rooting ratewas less efficient compared to in vitro rooting, better survivalrate was observed after soil acclimatization. Present studysuggested that above micropropagation techniques can beused for rapid multiplication as well as in vitro or in vivoconservation of the species.

針葉樹 幼苗의 모잘록병 發生에 미치는 環境要因의 影響

韓茂錫,尹貞求 충북대학교 농업과학기술연구소 1990 農業科學硏究 Vol.8 No.2

The pathogens causing damping-off of coniferous seedlings were isolated from diseased seedlings of the pine, larch and soil of seedlings bed. After isolating and identifying the causal fungi, ecological factors such as temperature affecting mycelial growth, pathogenicity and conidia germination were investigated. In addition, outbreak of damping-off by different growing stages were also investigated. The results obtained were as follows. 1. The causal pathogens identified were Fusarium moniliforme, F. acuminayum, F. graminearum, the unidentified Fusarium spp. and confirmed their pathogenicity on the seedlings of Pinus densiflora and Pinnus thunbergii. 2. Fusarium moniliforme showed the highest frequency among pathogens isolated from diseased seedings, whereas in the soil, F. acuminatum and F. sp. (8706) showed higher frequency than the others. 3. The optimum temperature for mycelial growth the Fusaria was 25℃~30℃, mycelial growth of every species was discontinued at 5℃, the optimum PH for mycelial growth was at 5.5 to 7.5. 4. Optimum temperature for the conidial germination was at 25℃. At 100% of relative humidity the conidial germination was above 94%, but decreasing trend was shown at less than 90%. 5. The percentage of the diseased seedlings in Pinus densiflora and Larx kaempferi by the fertilizer application were not remarkably different, but N fertilizer showed a positive correlation. 6. The outbreak percentage of diseased seedlings on Pinus densiflora and Larix kaempferi was a negative trend according to their age.

에세폰 처리에 의한 백합나무 클론묘의 조기 목질화 유도

한무석,김용욱,박소영,문흥규,권미 한국임학회 2011 산림과학 공동학술대회 논문집 Vol.2011 No.-

멸종위기식물 피뿌리풀의 기내증식

한무석,문흥규,강영제,김원우,강병서,변광옥,Han, Mu-Seok,Moon, Heung-Kyu,Kang, Young-Jae,Kim, Won-Woo,Kang, Byung-Seo,Byun, Kwang-Ok 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.1

멸종위기에 직면한 피뿌리풀 (Stellera roseaN.)의 기내증식법을 개발하고자 액아 마디를 MS 배지에 BAP와 zeatin을 처리하여 다경 (multiple shoot)를 유도하고 기내발근에 미치는IBA및 NAA처리 효과를 조사하였다. 액아 마디로부터 다경유도는 BA가 현저히 양호한 반면 줄기의 생장은 zeatin이 BA보다 효과적이었다. 증식된 줄기로부터 기내 발근은 오옥신 처리로 가능하였으나 발근율은 대체로 저조하였고 오옥신의 전처리 기간에 따라 차이를 보였다. IBA가 NAA보다 다소 좋은 발근효과를 보였고 1.0mg/L 농도로 15일간 배양시 30%까지 발근되었다. NAA역시 처리농도 및 처리기간에 따라 발근율에 차이를 보였다. 발근묘는 인공토양에서 51%가 활착되어 정상생장이 가능하였다. 이상의 결과는 발근 및 순화조건을 좀더 개선하면 피뿌리풀의 효율적인 기내번식이 가능함을 시사해준다. In order to develop an efficient micropropagation technique for an endangered species, Stellera rosea N., stem node cultures were conducted on MS medium supplemented with cytokinins. Generally, BA was better than zeatin on shoot proliferation from stem nodes, whereas zeatin showed more effective on shoot elongation. In vitro rooting of shoots was achieved by application of an auxin pre-culturing method. Overall rooting rate was relatively low and differed depending on the culture period. Pre-culturing of shoots for 15 days at 1.0mg/L IBA revealed a slightly better rooting efficiency reaching 30% rooting rate than NAA. Root induction rate by NAA also varied with concentration of NAA and culture periods. Total 51% of the rooted plantlets survived on artificial soil mixture and grew normally without any distinct morphological variation. The results suggest that the endangered Stetllera plants are propagated via in vitro culture system, but still need to more study for the improvement of rooting and acclimatization of the plantlets in soil.

24년생 루브라참나무의 생장과 재질에 관한 연구: 물리·역학적 성질

한무석,이창준,박봉석,김병로 韓國木材工學會 2014 목재공학 Vol.42 No.3

종자의 산지가 다른 루브라참나무(24년생)를 대상으로 물리 및 역학적 관계를 조사하여 생장속도와 목재의 재질과의 관계를 규명하였다. 생재함수율은 변재보다 심재가 높은 것으로 나타났고, 산지 간에 차이를 나타냈다. 비중의 경우 심재는 생장이 좋을수록 작아지는 경향을 보였고, 변재는 생장이 좋을수록 커지는 관계를 나타냈다. 전수축률은 산지간 차이를 나타냈으며, 생장이 좋을수록 수축률과 T/R율은 높아지는 것으로 나타났다. 압축영계수, 휨강도와 휨영계수 및 충격강도는 산지 간 차이를 보였으며, 생장이 좋을수록 낮아지는 경향을 보였다. 전단강도는 산지 간 차이를 보였으며, 생장이 좋을수록 커지는 경향을 보였다. 경도는 세 단면에서 산지 간 차이를 보였으며, 생장이 좋을수록 커지는 경향을 나타냈다. 5종의 산지가 다른 루브라참나무 중 Bancroft산 종자의 경우가 가장 좋은 수고생장을 보이고, 세 번째의 흉고직경을 나타내며, 압축, 휨, 경도, 전단, 충격강도 모두 우수하여 생장과 재질면을 고려했을 때 현시점에서 가장 우수한 품종으로 판단된다. Relationship between growth rate and wood quality was investigated by physical and mechanical properties with Quercus rubra (24 years old) from five different origin of apricot. In greenwood moisture content, sapwood had higher moisture content than heartwood, and there was difference among different origin of apricot. There were different specific gravity of wood among different origin of apricot. Compared with higher growth rate with higher specific gravity in sapwood, opposite trend was observed in heartwood. There were difference in shrinkage based on origin of apricot, and higher growth rate wood had higher shrinkage and T/R ratio. Compression Young's modulus, bending strength, bending Young's modulus, and compact strength was difference among different origin of apricot. Higher growth rate wood had higher tensile strength, and also there was difference amont different origin of apricot. In hardness, 3 different directions had all difference among different origin of apricot, and higher growth rate wood showed higher hardness than others. Based on overall physical and/or mechanical properties and growth rate, apricot from Bancroft was best quality in current.

학술연구논문 포스터발표 : 2분과: 수목생리, 임목육종, 생명공학 ; 전북완주 지역에서 36년생 테다소나무의 12개 산지간 생장특성

한무석,유근옥,이주환,배은경,김인식,이재천 한국임학회 2013 한국임학회 학술발표논문집 Vol.2013 No.-

참깨 엽화병의 발생과 파이토플라스마의 검출

한무석,노은운,윤정구 한국식물병리학회 1997 Plant Pathology Journal Vol.13 No.4

희귀 수종 시로미의 기내 증식

한무석,강영제,김원우,강병서,고정군,변광옥 한국산림과학회 2004 한국임학회 정기학술발표논문집 Vol.2004 No.-