Haloperidol 및 Sulpiride 투여후 백서 뇌내 Dopamine 수용체 결합력의 변화

한규희,안윤영,Hahn, Kyu-Hee,Ahn, Yun-Young 대한생물정신의학회 1995 생물정신의학 Vol.2 No.1

흰쥐에 항정신병약물인 haloperidol과 sulpiride를 장기간 투여한 뒤 줄무늬체와 후결절조직에서의 DA 수용체 의 변동에 대해 조사하였다. 이와 함께 apomorphine 투여에 따른 상동행동점수도 평가하였다. 흰쥐에 haloperidol(0.5mg/kg/day) 이나 sulpiride(40mg/kg/day)를 4주간 투여하고, 3일간의 약물 배설기간을 거친 후에 apomorphine(0.5mg/kg)을 투여하여 상동행동점수를 평가하였다. Haloperidol을 투여한 군에서는 대조군이나 sulpiride를 투여한 군에 비하여 상동행동에 대한 접수가 높게 나타났고, sulpiride를 투여한 군에서는 대조군과 유사한 정도의 상동행동을 나타내었다. 한편 줄무늬체 조직의 [$^3H$]spiperone의 결합에 대한 포화실험에서는 대조군에 비해 haloperidol이나 sulpiride를 투여한 군에서 최대결합치가 유의하게 증가되었다. 해리상수는 sulpiride투여시 줄무늬체에서 유의하게 감소되어 있었다. Sulpiride를 투여했을때 haloperidol 투여 시와 마찬가지의 DA수용체의 증식이 일어나지만, sulpiride 투여군에서만 상통행동 접수가 낮은 것으로 볼 때, 뇌의 DA계에서 sulpiride가 haloperidol과는 다르게 작용한다는 것을 간접적으로 시사하고 있다. 후결절에서는 sulpiride와 haloperidol을 투여한 양군에서 모두 최대결합치가 현저하게 증가되어 있었다. 또한 해라상수도 양군의 후결절에서 유의하게 증가되었다. 더욱이, 대조군은 후결절에서의 해리상수가 37.5pM, 줄무늬체에서는 86.2pM로 나타나, 후결절에서 줄무늬체에서보다 친화도가 두배 이상 높게 측정되었다. 위의 결과로 미루어 볼 때 sulpiride는 후결절에서 DA수용체의 증식을 유도할 뿐만 아니라 높은 친화력을 가진 몇몇 DA수용체 아형과도 상호작용을 한다는 것을 나타낸다. 또한 대조군에서 줄무늬체와 후결절에서의 친화도가 다른 점은, [$^3H$]spiperone에 반응한 DA수용체 아형은 줄무늬체와 후결절에서 각기 다르다는 점을 시사하고 있다. The effects of chronic treatment with haloperidol and sulpiride on the binding capacities of dopamine(DA) receptor were examined in rat striatum and olfactory tubercle. Additionally, the stereotypy scores were assessed after apomorphine administration. Rats were treated with haloperidol(0.5mg/kg/day) or sulpiride(40mg/kg/day) for four weeks. Apomorphine(0.5mg/kg) was injected after three-day washout from neuroleptics, and stereotypy scores were assessed. Haloperidol group showed high scores of stereotyped behavior in comparison with control and sulpiride groups. With control group, sulpiride group displayed similar stereotyped behaviors. Saturation analysis of the binding of [$^3H$]spiperone to striatal membranes showed that the Bmax of haloperidol and sulpiride groups increased significantly in comparison with that of control group. The $K_D$ decreased significantly after sulpiride treatment in striatum. Although sulpiride produces the same proliferation of DA receptor, the low stereotypy scores of sulpiride group indirectly suggest that sulpiride acts differently from haloperidol in brain DA system. The Bmax increased remarkably following both treatment with haloperidol and sulpiride in olfactory tubercle. Also, the increase in $K_D$ was significant after treatment with haloperidol and sulpiride in olfactory tubercle. Moreover, the $K_D$ of control group in olfactory tubercle was more than twice the $K_D$ of control group in striatum. The $K_D$ was 86.2 in striatum and 37.5 pM in olfactory tubercle. The present finding indicates that sulpiride also induces the proliferation of DA receptor in olfactory tubercle and may interact with some DA receptor subtype with high affinity profile. The different affinities of the control groups of striatum and olfactory tubercle suggest that striatal DA receptor subtypes labeled by [$^3H$]spiperone could differ from those of olfactory tubercle.

안진의 해석

한규희 대한평형의학회 2012 Research in Vestibular Science Vol.11 No.-

caused by retinal slip. Observation of nystagmus is the core examination in patients

Haloperidol 投與後의 白鼠 線條體內의 Dopamine 前後接合部位 受容體의 變動

韓圭熙,禹誠一 서울醫大 1990 精神醫學 Vol.15 No.4

정신분열병환자의 혈청내 Haloperidol치에 관한 예비적 연구 : HPLC를 이용한 Measured by High Performance Liqiud Chomatography

韓圭熙,延炳吉,楊秉煥,金喆珪,金二泳 漢陽大學校 精神健康硏究 1985 精神健康硏究 Vol.3 No.-

Bone Anchored Hearing Aid(BAHA)를 이용한 청각 재활

한규희,김희진,장정훈,유재철,김영호,이준호,오승하,김종선,장선오 대한이비인후과학회 2010 대한이비인후과학회지 두경부외과학 Vol.53 No.12

Background and Objectives Bone anchored hearing aid (BAHA) is an alternative method applicable to patients with chronic draining ear, congenital aural atresia and single sided deafness, who cannot benefit from conventional air conduction hearing aids. The objective of this study was to evaluate the experience of 14 patients who underwent BAHA surgery. Subjects and Method We retrospectively reviewed the medical records of 14 patients who underwent BAHA surgery. Preoperative pure tone air and bone conduction thresholds and air-bone gap, postoperative BAHA-aided thresholds were measured. Hearing improvement as a result of implantation and complications related to implant were evaluated. Results The most common indication for BAHA was congenital aural atresia (8 patients) and the rest consisted of chronic otitis media (3 patients) and unilateral sensorineural hearing loss (3 patients). The average threshold improvement with BAHA was 40 dB and 34 dB in patients with congenital aural atresia and chronic otitis media, respectively. Patients with unilateral hearing loss had a postoperative aided threshold of 25 dB. Complications were limited to the periabutment skin problem in two patients. One patient received revision surgery replacing the diseased skin with split-thickness skin graft from a thigh and the other patient received surgery to remove the abutment and the wound was closed with rotation flap, leaving the fixture underneath the skin. Conclusion BAHA could be one of the safe and reliable treatment options available for auditory rehabilitation. Systematic evaluation for candidate selection might be needed to increase hearing gain and decrease co-morbidity. Korean J Otorhinolaryngol-Head Neck Surg 2010;53:755-60

同位元素 受容體 結合方法을 利用한 血淸內 抗精神病 藥物의 濃度 測定

韓圭熙,禹誠一 서울大學校 醫科大學 精神科學敎室 1991 精神 醫學 Vol.16 No.4

폐쇄성 수면무호흡증 환자에서 수면무호흡 정도, 수면 및 기분관련 척도, 수면중 활동도 간의 연관성

한규희,소민아,하지현,유승호,유재학,박두흠,Han, Kyu-Hee,Soh, Min-Ah,Ha, Jee-Hyun,Ryu, Seung-Ho,Yu, Jae-Hak,Park, Doo-Heum 대한수면의학회 2011 수면·정신생리 Vol.18 No.2

목 적 : 본 연구는 폐쇄성 수면무호흡증후군 환자에서 수면무호흡지수, 수면관련 척도, 기분관련 척도, 그리고 수면중 신체활동도 간의 연관성을 보고자 하였다. 방 법 : 폐쇄성 수면무호흡증후군을 보이는 122명(평균연령 $43{\pm}11$세)의 남자환자를 선정하였다. 폐쇄성 수면무호흡증후군 진단은 수면무호흡지수>5인 경우로 하였고, 수면무호흡지수의 평균은 $39.6{\pm}26.0$이였다. 수면관련척도로 Stan-ford Sleepiness Scale(SSS), Epworth Sleepiness Scale(E-SS), Pittsburg Sleep Quality Index(PSQI), Morningness-Ev-eningness Scale(MES)이 사용되었고, 기분관련척도로 Beck Depression Inventory(BDI), Beck Anxiety Inventory(BAI), State-Trait Anxiety Inventory(STAI) I, II, Profile of Mood States(POMS)가 사용되었다. 수면중 활동도를 측정하기 위해 양측 손목에 손목 액티그라프(wrist actigraphy, WATG)를 착용한 채 1일밤 야간수면다원검사(nocturnal polysom-nography, NPSG)를 시행하였다. WATG에서 산출된 지수는 총활동도(total activity score), 평균활동도(mean activity score) 및 분절지수(fragmentation index)였다. 수면무호흡지수, 수면 및 기분관련척도, 수면중 활동도 간의 상관관계를 분석하였다. 결 과 : ESS는 PSQI, BDI, BAI, STAI I, II와 각각 유의한 양의 상관관계를 보였다(p<0.01). SSS는 PSQI, BAI와 유의한 양의 상관관계를 보였다(p<0.05, p<0.01). BAI는 총활동도, 평균활동도 및 분절지수(fragmentation index)와 유의한 양의 상관관계를 보였다(p<0.05, p<0.01, p<0.05). 수면중 총활동도는 ESS, BAI와 각각 유의한 양의 상관관계를 보였다(p<0.05). 수면 분절지수는 ESS, PSQI, BAI와 유의한 양의 상관관계를 보였다(p<0.05, p<0.01, p<0.05). 수면무호흡지수는 수면 및 기분관련 척도와 유의한 상관관계를 보이지 않았다. 결 론 : 주간 졸리움을 호소하는 정도가 수면무호흡증의 심한 정도와 연관이 있기 보다는 오히려 야간수면 만족도, 우울 및 불안의 정도, 수면중 활동도와 더 연관성이 있었다. Objectives: This study aims to analyze the association between the severity of sleep apnea, sleep and mood related scales, and activity during sleep in obstructive sleep apnea syndrome (OSAS) patients. Methods: 176 drug-free male patients confirmed as OSAS (average age=$43{\pm}11$ years) were selected through nocturnal polysomnography (NPSG). OSAS was diagnosed with apnea-hypopnea index (AHI) >5, mean AHI was $39.6{\pm}26.0$. Sleep related scales were Stanford Sleepiness Scale (SSS), Epworth Sleepiness Scale (ESS), Pittsburg Sleep Quality Index (PSQI) and Morningness-Eveningness Scale (MES). Mood related scales were Beck Depression Inventory (BDI), Beck Anxiety Inventory (BAI), State-Trait Anxiety Inventory (STAI) I, II and Profile of Mood States (POMS). NPSG was performed overnight with both wrist actigraphy (WATG). Parameters produced from WATG were total activity score, mean activity score and fragmentation index. We analyzed the correlation between each scale, AHI scored from NPSG and activity score analyzed from WATG. Results: ESS showed significant positive correlation with PSQI, BDI, BAI and STAI I, II, respectively (p<0.01). SSS showed significant positive correlation with PSQI and BAI (p<0.05, p<0.01). BAI showed significant positive correlation with total activity score, mean activity score and fragmentation index (p<0.05, p<0.01, p<0.05).Total activity score showed significant positive correlation with ESS and BAI, respectively (p<0.05). Fragmentation index showed significant positive correlation with ESS, PSQI and BAI (p<0.05, p<0.01, p<0.05). AHI, indicator of sleep apnea is showed no significant correlation with each sleep and mood related scale. Conclusion: The degree of daytime sleepiness tends to be associated with night sleep satisfaction, depression and anxiety, and the activity during sleep rather than the severity of sleep apnea.

同位元素 受容體 結合方法을 利用한 血淸內 抗精神病 藥物의 濃度 測定

韓圭熙,禹誠一 서울醫大 1991 精神醫學 Vol.16 No.4

Bromocriptine이 백서 뇌의 Dopamine 수용체에 미치는 영향에 관한 연구

한규희,박철수,손진욱,김연웅,김충원 서울대학교 의과대학 정신과학교실 1988 서울醫大 精神醫學 Vol.13 No.1

인공와우이식 후 발생한 반복적 현훈

한규희,홍성광,송창면,구자원 대한평형의학회 2009 Research in Vestibular Science Vol.8 No.1

Post-operative dizziness and vertigo have been variously reported from 30 to 60% after cochlear implantation. There have been reported that delayed vertigo is the most common type and characterized by similarities with dizziness in Meniere’s syndrome. Authors experienced a man who had delayed onset of vertigo, which developed six months after cochlear implantation on the left side. Postoperative left caloric response was decreased in both warm and cold stimulation. The direction of spontaneous nystagmus during spell was right side with torsional down beat and definite catch up saccades were observed during head thrust test on the plane of the left horizontal canal and posterior canal, which was resolved on the next day. These episodic and recurrent spells of vertigo might suggest that the hydropic changes of labyrinth corresponding to the pathology of Meniere’s disease occurred in this patient. Post-operative dizziness and vertigo have been variously reported from 30 to 60% after cochlear implantation. There have been reported that delayed vertigo is the most common type and characterized by similarities with dizziness in Meniere’s syndrome. Authors experienced a man who had delayed onset of vertigo, which developed six months after cochlear implantation on the left side. Postoperative left caloric response was decreased in both warm and cold stimulation. The direction of spontaneous nystagmus during spell was right side with torsional down beat and definite catch up saccades were observed during head thrust test on the plane of the left horizontal canal and posterior canal, which was resolved on the next day. These episodic and recurrent spells of vertigo might suggest that the hydropic changes of labyrinth corresponding to the pathology of Meniere’s disease occurred in this patient.