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한규삼,오병관,Han Kyu-Sam,Oh Byoung-Kwan 대한수의사회 1984 대한수의사회지 Vol.20 No.5
The authors made a survey infestation rate of endoparasites in wild .animals ( deer 68 heads and wild boar 88 heads) at Chon-Buk province. The results are summarized as follows; 1. Infection rate of species in deer Fasciola hepatica $22.8\%$ Oe
한규삼,Han Kyu-sam 대한수의사회 1986 대한수의사회지 Vol.22 No.10
A total of 1.252 quarters milk sample (998 from 267 cows of 37 herds and 254 of received from dairyfarms) in Chunbuk province were classified as clinical and subclinical group by CMT and RBU test. Of these positive sample, the mastitic agents were isolate
한국산(韓國産)메기(Silurus asotus)의 질병(疾病)에 관(關)한 연구 Aeromonas hydrophila에 의한 피부(皮膚) 궤양병(潰瘍病)
한규삼 ( Kyu Sam Han ),최인렬 ( In Yeol Choi ),배정준 ( Joung Joon Bae ),김영길 ( Young Gill Kim ),이근광 ( Keun Kwang Lee ),김영진 ( Young Jin Kim ) 한국가축위생학회 1993 韓國家畜衛生學會誌 Vol.16 No.2
The Aeromonas strains were isolated from diseased cultured catfsh(Silurus asotous) in the ponds near by Gochang of Chunbuk. The present isolates were identified as A. hydrophila based on their biological and biochemical characteristics. The isolates of A. Hydrophila were named GC-1, GC-2, GC-3, GC-4 and GC-5, Five strains were grew optimally at temperatures 35℃, pH 7.5 and in 0.5 to 1% NaCl, This bacterium(GC-1) was injected into health catfish in order to prove the causative agent of ascites and haemorrhagic ulcers. The symptoms in catfish infected by this challenge method were observed to be very similar to the symptoms of a natural infection, but controls did not show any abnormal symptoms during the experimental period. At 24 post-injection, the red spot developed around the injection site and the haemorrhagic ulcers was extended near the gill and ventral fin. Five strains were tested for drug sensitivity by plate method. All strains were sensitivity to gentamicin and resistant to penicillin, streptomycin and tetracycline.
뱀장어에서 분리한 부정형(不定形) Aeromonas salmonicida EL-1의 일부 특성과 병원성에 관한 연구
한규삼 ( Kyu Sam Han ),최인열 ( In Yeol Choi ),배정준 ( Joung Joon Bae ),신성일 ( Sung Il Shin ),김영진 ( Young Jin Kim ),김영길 ( Young Gill Kim ),이근광 ( Keun Kwang Lee ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.1
A partial characteristics and pathogenicity of Aeromonas salmonicida strain isolated from diseased cultured eel were studied. On the biochemicial characteristics of the isolate strain was classified into atypical Aeromonas salmonicida: the characteristics of the isolate strain differed from those of the subspecies of Achromogenes, masoucida and salmonicida. It strain was named atypical Aeromonas salmonicida EL-1 and it grew at optimal pH 7.5 and 1% Nacl. In the pathogenicity test, at one to two days post infection, the motality of artificially infected eels with 1×10(8) and 1.0×10(7)cfu/fish group was 100%, and at three days pos-infection, in the 1.0×l0(6)cfu/fish infected group, the epizootic haemorrhagic ulcer was developed, and haemorragic red sports were formed around abdomen and fins and its died(40%). Total cell protein peptide bands of the A. salmonicida EL-1 were presented betweeon 11.8~102 Kd in molecular weight by the electrophoretic analysis and appeared 28 bands in all. Drug sensitivity of atypical A. salmonicida EL-1 strain was sensitivity to chlorampenicol, gentamicin, kanamycin, streptomycin and sulfamethoxazole/trimethoprim and intermidiate to ampiclin and neomycin resistant to cephalotin, erythromycin, lincomycin and tetracycline.
韓國産메기(Silurus asotus)의 疾病에 關한 연구 Aeromonas hydrophila에 의한 皮膚 潰瘍病
한규삼(Kyu-Sam Han),최인열(In-Yeol Choi),배정준(Joung-Joon Bae),김영길(Young-Gill Kim),이근광(Keun-Kwang Lee),김영진(Young-Jin Kim) 군산대학교 수산과학연구소 1994 군산대학교 수산과학연구소 연구논문집 Vol.2 No.-
The Aeromonas strains were isolated from diseased cultured catfsh(Silurus asotous) in the ponds near by Gochang of Chunbuk. The present isolates were identified as A. hydrophila based on their biological aud biochemical characteristics. The isolates of A. Hydrophila were named GC-l, GC-2, GC-3, GC-4 and GC-5. Five strains were grew optimally at temperatures 35℃. pH 7.5 and in 0.5 to 1% NaCㅣ, This bacterium(GC-l) was injected into health catfish in order to prove the causative agent of ascites and haemorrhagic ulcers. The symptoms in catfish infected by this challenge method were observed to be very similar to the symptoms of a natural infection, but controls did not show any abnormal symptoms during the experimental period. At 24 post-injection, the red spot developed around the injection site and the haemorrhagic ulcers was extended near the gill and ventral fin. Five strains were tested for drug sensitivity by plate method. All strains were sensitivity to gentamicin and resistant to penicillin, streptomycin and tetracycline.
담수어류에서 Argulus japonicus의 실험감염에 관한 연구
한규삼 ( Kyu Sam Han ),정영미 ( Young Mee Jung ),박태욱 ( Tae Wook Park ),임채웅 ( Chae Woong Lim ),송희종 ( Hee Jong Song ),도홍기 ( Hong Ki Do ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.4
Argulus spp, a small group of parasitic curstaceans called Branchiura, is particularly important in freshwater acquaculture. We investigated the infection of Argulus to several different species of fishes and therapy effect of masoten. Argulus japonicus was collected from Okjeong lake, Chonbuk province and experimen-tally infected to freshwater fishes from July to August 1998. The results were as follows; 1. The shape of a Agulus is an oval and dorsoventrally flattened, with first maxillae and second maxillae, and four pairs of legs. 2. The size of the adult stage of A japonicus was 3.2-5.0×2.3-2.9mm in female and 1.5-3.1×1.6-2.9mm in male. 3. In the trial of experimental infection with A japonicus to non-scaled fishes, Parasilurus asotus and Ictalurus punctalus, were infected and some of them died after severe infection. However, A japonica was not infected at all. 4. Masoten treatment for A japonicus was effective at 0.2 ppm once a day for 3 days and parasites on the surface of fishes disappeared after five days.
한규삼 ( Kyu Sam Han ),이성효 ( Sung Hyo Lee ),서문정 ( Moon Jeong Sub ),오언평 ( Un Pyong Oh ),채효석 ( Hyo Seok Chai ),유혜진 ( Hye Jin You ),임채웅 ( Chae Woong Lim ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.2
This article reports a spontaneous thoracic teratoma in a laying hen (Deklb brown warron). In this case, a 40 days old hen was submitted for necropsy as part of an investigation into a flock problem suspected Marek`s disease to Chonbuk Veterinary Service Laboratory in 1983. On the gross finding, mass was in the cranial subpulmonary cavity and attached to the vertebral column. It contained fully developed contour feathers. Histologically, feathers were arised from feather follicles complete with arrector plumi muscle, nerve, vessel and mucous gland composed with simple tall columnar epithelium. The outer surface of mass was lined by keratinized or nonkeratinized stratified squamous epithelium. It converted to pseudostratified ciliated columnar epithelium in some area. There were lymphocyte infiltration around gland tissue and eosinophilic intranuclear inclusion bodies in nonkeratinized epithelium. This thoracic teratoma was composed of ectodermal origin, squamous epithelium and nerve, and endodermal origin, mucous gland. This case in laying hen has never been reported in the literature in the world.
토끼 간콕시듐(Eimeria stiedae) 감염 증례
한재철 ( Jae Cheol Han ),한규삼 ( Kyu Sam Han ),이성희 ( Sung Hee Rhee ),마쓰다기꾸 ( Kiku Matuda ),임병무 ( Byung Moo Rim ),임채웅 ( Chae Woong Lim ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.1
Hepatic coccidiosis was occurred in a rabbit farm in Chonbuk province. Clinically, rabbits showed anorexia, diarrhea, dehydration, and depression, subsequently died 3-5 days after onset of clinical signs. Grossly, multifocal white spots or lines on the liver surface were observed. Histopathologic lesions included hyperplasia of bile duct epithelium with infiltration of inflammatory cells such as plasma cells and granulocytes, which represents chronic pericholangitis. Different developmental stages of Eimeria stiedae were observed inside the epithelium of biliary system. This is the case of hepatic coccidiosis in rabbits.
Aspergillus sp 감염에 의한 닭 복강장막의 육아종성 결절 발생 례
육현수 ( Hyun Soo Yuk ),한규삼 ( Kyu Sam Han ),이성재 ( Sung Jae Lee ),임채웅 ( Chae Woong Lim ),도홍기 ( Hong Ki Do ),임병무 ( Byung Moo Rim ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.4
Aspergillosis, caused by infection with Aspergillus fumigatus and less commonly by other Aspergillus species, is a prevalent and costly respiratory disease of poultry. In a flock of chicks, the number of birds, 4~5 months old, had become gradually emaciated and subsequently died. Gross necropsy revealed multiple granulomatous masses on the abdominal serosa. The masses, 4~15mm in size, were attached on mainly intestinal wall. Also, the smaller masses in size were on mesentery and pancreas. However, only a few small white nodules were scattered throughout liver and lung in few samples. Microscopically, the mass were granulomatous with a central area of necrosis containing numerous septate, branched fungal hypae consistent with Aspergillus sp. These were surrounded by macrophages, giant cells, lymphocytes and fibrous tissues. Nodular lesions of liver and lung were seemed to spread hemotogenously from intestine and the possible route of infection was speculaled by oral. This report is a Aspergillus-induced granuloma limited to the serosa of abdominal cavity, especially of intestinal wall.
Studies on leucocytozoonosis of chickens in Honam districts
임병무,서민석,이재구,이주묵,김영진,최인열,한규삼,Rim, Byung-moo,Suh, Min-seuk,Rhee, Jae-ku,Lee, Joo-muk,Kim, Young-jin,Choi, In-yeol,Han, Kyu-sam The Korean Society of Veterinary Science 1994 大韓獸醫學會誌 Vol.34 No.1
1992-1993년 호남지방의 4개 양계장에서 처음으로 닭의 Leucocytozoon caulleryi 자연감염증을 보이는 87수를 검색하였는데, 임상증세와 혈액내 merozoite 및 gametocyte 검출 그리고 내장기관으로부터 schizont의 발견으로 본 질병을 확증할 수 있었다. 본 감염증은 일년 중 6월 하순부터 9월 중순까지에 발견되었으며, 자연감염된 혈액(merozoite포함)을 미감염된 닭에 접종하여 인공감염상을 볼 수 있었다. 자연감염후 회복된 닭중 1수는 12월에 간에서, 또 다른 1수는 다음해 2월에 심장 근육에서 schizont가 발견되어 본 감염증의 재발이나 동절기를 지나는 장기간 감염 가능성을 보여 주었다. 본 원충의 gametocyte 추출물에서 특이 항원 항체 반응을 보이는 polypeptide는 50.1kD이었다. In the year 1992/93 leucocytozoonosis could be first diagnbosed in 87 chickens of 4 chicken farms in Honam districts. The diagnosis was confirmed by detection of the blood merozoites or gametocytes and histological finding of the schizonts from various organs with some clinical signs. Cases of leucocytozoonosis only occurred from the end of June to the middle of September. Artificial infection could be observed by means of inoculation of infected blood merozoites. The schizonts were found in the liver and cardiac muscle of the different chickens recovered from the natural infection, respectively, in September and next February. Thus the relapse or long-term infection in cold seasons might be possible. The unique gametocyte antigen polypeptide was 50.1 kD.