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송이버섯 추출액의 항균력 확인과 저염젓갈 생산에 적용 가능성
김진성(Jin-Seong Kim),박재범(Jae-Bum Park),장승원(Seung-Won Jang),권덕호(Deok-Ho Kwon),장미희(Mi-Hee Jang),이미옥(Mi-Ok Lee),하석진(Suk-Jin Ha) 한국생물공학회 2015 KSBB Journal Vol.30 No.5
송이버섯 추출액의 박테리아에 대한 항균력을 측정하기 위하여 최소저해농도를 확인한 결과 젓갈 제조시 빈번하게 발생하는 부패균, 어병원인 균등 9가지 박테리아에 대해 성장이 저해되는 것을 확인하였다. 확인된 송이버섯 추출액의 항균력을 저염 젓갈의 제조 공정에 적용하기 위해 식품보존제인 사과산나트륨과 아질산나트륨을 함께 첨가하여 S. aureus에 대한 최소저해농도를 확인하였다. 그 결과 사과산나트륨과 아질산나트륨의 최소저해농도가 각각 0.25 g/L와 0.025 g/L로 크게 낮아지는 것을 확인할 수 있었다. 본 연구를 통해 항균력을 갖는 송이버섯 추출액을 첨가함으로써 일반적으로 첨가되는 식품보존제의 농도를 크게 낮출 수 있었을 뿐 아니라 송이버섯의 다양한 기능성이 추가된 고급화된 저염 젓갈을 개발할 수 있을 것으로 예상된다. The antibacterial activity from Tricholoma matsutake extracts were confirmed by minimum inhibitory concentration (MIC) test against various bacteria. Tricholoma matsutake extracts was applied for manufacturing low salted Jeot-gal with sodium DL-malate and sodium nitrite as food preservatives. Due to antibacterial activity from Tricholoma matsutake extracts, MIC of sodium DL-malate and sodium nitrite were significantly reduced to 0.025 g/L and 0.25 g/L, respectively. As a result of this study, a premium low salted Jeot-gal can be developed with low concentration of food preservatives by adding Tricholoma matsutake extracts.
교반속도 최적화를 통한 Kluyveromyces marxianus 36907-FMEL1 로부터 고온에서의 자일리톨 생산성 증진
김진성 ( Jin-seong Kim ),박재범 ( Jae-bum Park ),장승원 ( Seung-won Jang ),권덕호 ( Deok-ho Kwon ),홍억기 ( Eock Kee Hong ),신원철 ( Won Cheol Shin ),하석진 ( Suk-jin Ha ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 한국미생물·생명공학회지 Vol.45 No.1
최근 모균주인 K. marxianus ATCC 36907 보다 xylose reductase의 활성이 증대된 돌연변이 K. marxianus 36907-FMEL1 균주를 개발하였다. 본 연구에서는 효율적인 xylitol의 생산을 위해 발효기를 이용하여 교반속도에 따른 효과를 확인하였다. 30°C조건에서 발효를 진행한 결과 K. marxianus 36907-FMEL1 균주는 400 rpm의 교반속도에서 가장 높은 수율 (0.57 g/g)과 생산성(0.64 g· l<sup>-1</sup>·h<sup>-1</sup>)을 확인할 수 있었다. 흥미롭게도 온도 조건을 40℃로 증가하여 발효를 진행한 결과 agitation 조건 최적화를 통해 수율과 생산성이 각각 21% (0.64 g/g)와 58% (0.90 g· l<sup>-1</sup>·h<sup>-1</sup>) 증가하였다. Recently, we isolated the mutant Kluyveromyces marxianus 36907-FMEL1, which demonstrated improved xylose reductase activity as compared to the parental strain, K. marxianus ATCC 36907. Effects of agitation conditions on xylitol production were verified using a bioreactor system. Under an agitation speed of 400 rpm, K. marxianus 36907-FMEL1 exhibited the highest xylitol yield (0.57 g/g) and productivity (0.64 g·l<sup>-1</sup>·h<sup>-1</sup>) at 30℃. When the fermentation temperature was increased to 40℃, interestingly, xylitol yield and productivity were found to be increased to 21% (0.64 g/g) and 58% (0.90 g·l<sup>-1</sup>·h<sup>-1</sup>), respectively, under the optimized agitation conditions.
유도적 돌연변이 유발 방법을 통한 1-ethyl-3-methylimidazolium acetate에 대해 내성을 갖는 돌연변이 효모 선별
이유진 ( Yoo-jin Lee ),권덕호 ( Deok-ho Kwon ),박재범 ( Jae-bum Park ),하석진 ( Suk-jin Ha ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 한국미생물·생명공학회지 Vol.45 No.1
목질계 바이오 매스 전처리에 사용되는 ionic liquid는 전처리 후 100% 회수되지 않아 잔존하는 ionic liquid의 독성이 직접적으로 미생물 균주의 생육에 나쁜 영향을 미쳐 에탄올 발효의 수율 및 생산성을 저해하는 문제를 가지고 있다. 본 연구에서는 ionic liquid에 저해를 받지 않으며 높은 ethanol 생산 효율을 가진 균주를 얻고자 유도적 돌연변이 유발 실험을 진행하였다. 선별된 돌연변이 균주 D452-B2와 D452-S3는 3% [EMIM][Ac]가 포함된 배지에서 glucose 소비속도는 4.5 g·l<sup>-1</sup>·h<sup>-1</sup>와 4.4 g·l<sup>-1</sup>·h<sup>-1</sup>로 모균주인 S. cerevisiae D452-2 균주에 비해 6배 가량 증가하였으며, ethanol 생산성은 각각 1.99 g·l<sup>-1</sup>·h<sup>-1</sup>와 2.0 g·l<sup>-1</sup>·h<sup>-1</sup>로 27배 가량 증가하였다. Cellulosic biomass is a renewable source for biofuel production from non-edible biomass. An optimized pretreatment process is required for the efficient utilization of cellulosic biomass. Among various pretreatment processes, the use of ionic liquids has been reported recently. However, the residual ionic liquid after pretreatment acts as an inhibitor of microbial fermentation. Recently, we isolated mutant Saccharomyces cerevisiae strains resistant to the ionic liquid 1-ethyl-3-methylimidazolium acetate ([EMIM][Ac]) by using a directed evolutionary approach. When 3% [EMIM][Ac] was added to a medium containing 80 g/l of glucose, mutants D452-B2 and D452-S3 produced 35.6 g/l and 36.3 g/l of ethanol, respectively, for 18 h while the parental strain (S. cerevisiae D452-2) produced 1.3 g/l of ethanol. Thus, these mutant S. cerevisiae strains might prove advantageous when ionic liquids are used for biofuel production from cellulosic biomass.
손다진(Da-Jin Son),김종석(Jong-Seok Kim),박재범(Jae-Bum Park),권덕호(Deok-Ho Kwon),정형무(Hyung-Moo Jung),한상인(Sang-In Han),홍억기(Eock-Kee Hong),하석진(Suk-Jin Ha) 한국생물공학회 2018 KSBB Journal Vol.33 No.2
Multiple sclerosis is a chronic disorder commonly occurred in the central nervous system and is an autoimmune disease. Corticosteroids are used as a short-term treatment for multiple sclerosis, but they cause side effects. Therefore, interferon beta having antiviral and anti-inflammatory effects, is used for long-term treatment. Interferon beta-1a, produced in Chinese hamster ovary cells, is glycosylated upon biosynthesis and forms glycoform, which is similar to naturally occurring protein. However, its process has disadvantages such as slow cell growth, low yield and high contamination possibility. Also, recombinant Escherichia coli is widely used for the production of interferon beta-1b due to its rapid growth and easy process scale up, but it lacks post-translational modification, leading to low activity. In addition, the produced Interferon beta-1b might cause protein aggregation due to misfolding. Alternatively, yeast can be used as a production host possessing N-glycosylation activity. Therefore, the choice of expression system in the production of interferon beta should be carefully selected in relation to the quality and yield.
Kluyveromyces marxianus 균주를 이용한 3-Hydroxypropionic acid 생산
박재범(Jae-Bum Park),강경곤(Kyoung-Gon Kang),권덕호(Deok-Ho Kwon),하석진(Suk-Jin Ha) 한국생물공학회 2020 KSBB Journal Vol.35 No.4
Thermotolerant yeast, Kluyveromyces marxianus was applied for the production of 3-hydroxypropionic acid (3-HP), which is mainly used as a raw material for biodegradable polymers. The MCR (malonyl-CoA reductase) gene originated from the Chloroflexus aurantiacus strain was introduced into the engineered K. marxianus 17555-JBP2 strain showing improved xylose utilization in the previous study to develop the engineered K. marxianus JBP2_MCR strain. At 40℃, K. marxianus JBP2_MCR strain produced 0.14±0.00 g/L of 3-HP using xylose as a sole carbon source. As a result of measuring the enzymatic activity of malonyl-CoA reductase, the engineered K. marxianus JBP2_MCR strain exhibited 4.71 times higher MCR enzymatic activity than the parent strain. When 1.28 g/L malonyl-CoA was added into media, 1.58±0.18 g/L of 3-HP was produced by K. marxianus JBP2_MCR strain for 120 h.
홍유동(Yudong Hong),강경곤(Kyoung-Gon Kang),정재윤(Jae-Youn Jung),하석진(Suk-Jin Ha),임광석(Kwang Suk Lim) 한국생물공학회 2021 KSBB Journal Vol.36 No.2
Biopharmaceutical drugs have strong therapeutic effect in various diseases such as autoimmune diseases, rheumatism and cancer due to their target specific recognition and low side effects. In particular, the most representative antibodies among biopharmaceuticals are widely used for targeted drug delivery, diagnosis and treatment. Moreover, antibody-drug conjugate (ADC) have been developed by many researchers and biopharmaceutical companies. An antibody-drug conjugate, one of the new bio anticancer drugs is composed of an antibody, cytotoxic drug and chemical linker. Antibodies accurately recognize the target cancer, and drugs induce apoptosis or death of the cancer cells. Linkers stably connects the antibody and drug until ADC go to the target cancer cells. In order to develop an ADC, it is important to correctly configure each component. Recently, the 9th ADC was approved by food and drug administration (FDA) and a lot of development of new ADC is underway. The development of ADC is expected through the introduction of new antibodies including bi-specific and drugs with new anticancer mechanisms.