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Vibrio parahaemolyticus 표준 및 식품분리 균주에 대한살균소독제 유효성분별 감수성 평가
하상도,김용수,김형일,최현철,전대훈,이영자,김일진 한국식품위생안전성학회 2007 한국식품위생안전성학회지 Vol.22 No.2
95%), iodine (15 and 25 ppm), chlorine (100 and 200 ppm), quaternary amonium, acid, hydrogen peroxide, and per-oxide acetic acid against V. parahaemolyticus. Ten strains of V. parahaemolyticus isolated from Korean foods and 4strains of standard V. parahaemolyticus were compared for efficacies of various sanitizers and disinfectants by EN1276 method based on quantitative suspension test. Ethanol (75 and 95%), 25 ppm of iodine, 100 ppm of quaternaryamonium, 145 ppm of hydrogen peroxide and acid showed more than 5 log10CFU/mL reduction in both clean anddirty conditions. Tests result of chlorine (100 ppm) showed more than 5 log10Iodine (15 ppm) showed more than 5 log10CFU/mL reduction except 4 isolated and 1 standard V. parahaemolyticus inclean condition. Iodine (15 ppm) also showed under 5 log10CFU/mL reduction (0.93~3.73 log10CFU/mL) in dirty con-dition. Eleven hundred ppm of hydrogen peroxide was evaluated as weak sanitizer and disinfectant due to their0.99~4.79 log10CFU/mL reduction on both clean and dirty conditions. Consequently, ethanol, iodine (25 ppm), chlo-rine (200 pm), quaternary ammonium, acid and peroxide acetic acid were thought to be effective sanitizer and disin-fectant against
식품내의 미생물 분리를 위한 dryfilm 방법의 평가연구
하상도 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2
본 실험에서는 식품내 미생물을 분리하는 불편한 전통적 agar 배지법을 대처하기 위하여 간편하고 정확한 3M Petrifilm^TM을 이용한 dryfilm 방법을 비교, 평가하였다. 총균수 분리배지인 standard method agar (SMA)와 Petrifilm^TM standard method (PSM, 3M)는 P<0.05 범위에서 우유, 간 쇠고기, 탁주, 밀가루, 어묵의 다섯 가지 식품으로 부터의 균분리 능력에 있어 유의적인 차이가 없었다. 우유와 육류로부터 분리된 coliform 수와 E. coli 수는 brilliant green lactose broth (BGLB)에 의한 MPN법과 Petrifilm^TM coliform count (PCC, 3M)법을 사용했을 때 그리고 EC broth에 의한 MPN법과 Petrifilm^TM E. coli count(PEC, 3M)법을 사용했을 때 각각 P<0.05 범위에서 유의적으로 차이가 없었다. 우유로부터 분리된 coliform 수는 P<0.05에서 violet red bile agar(VRBA)와 PCC(3M)간에 유의적 차이을 보이지 않았으나 육고기 시료를 사용하였을 때 PCC(3M)는 VRBA에 비하여 유의적으로 적은 수의 coliform을 분리하였다. 탁주 시료내의 효모와 밀가루 시료내의 곰팡이를 분리하기 위한 배지인 acidified potato dextrose agar(APDA)와 Petrifilm^TM yeast & mold count(PYMC)를 비교하였는데, 균 분리능력에 유의적 차이를 보이지 않았다. 이 결과로 3M Petrifilm^TM을 이용한 dryfilm 방법이 제조시간이 길고, 불편한 전통적 agar 이용법의 좋은 대처방법으로 사용될 수 있다는 것을 알 수 있다. Dryfilm method by using 3M Petrifilm^TM has been examined to replace conventional agar method for isolation of microorganisms from foods. The objectives of the present study were to evaluate suitability of dryfilm method as a microbilal isolation method and to determine the effect of antimicrobial agent on dryfilm for isolation of microorganisms from foods. Five different foods, milk, ground beef, fishery surimi, Takju and wheat flour were used to isolate the natural microflora in foods and inoculated Escherichia coli. Standard method agar (SMA, Difco) and Petrifilm^TM aerobic count (PAC, 3M) were used to isolate total microorganisms from foods. Violet red bile aga (VRBA), brilliant green lactose bile (BGLB) broth and Petrifilm^TM coliform count (PCC, 3M) were used to isolate coliforms from foods. E. coli broth (EC broth) and Petrifilm^TM E. coli count (PEC, 3M) were used to isolate E. coli from foods. Acidified potato dextrose agar(APDA) and Petrifilm^TM yeast & mold count (PYMC, 3M) were used to isolate yeasts and molds from foods. Total aerobic plate counts isolated from five different foods by SMA and PAC (3M) were not significantly different each other at P<0.05 level and were highly correlated each other (≥0.96). Mugwort extract as an antimicrobial agent did not affect microbial enumeratiion of Dryfilm. Significantly higher number of coliform colonies were formed on VRBA than PCC (3M) form ground beef, but they were not significantly different in coliform colonies from milk samples. PCC (3M) and BGLB were not significantly different for enumeration of coliforms in milk and beef samples. Significantly higher number of E. coli were isolated by EC broth than PEC from ground beef, but these were not significantly different for enumeration of E. coli from milk. Yeast and mold counts isolated from Takju and wheat flour by APDA and PYMC (3M) were not significantly different at P<0.05 level. These data indicate that dryfilm method by using 3M Petrifilm^TM can be successively used as an alternative to conventional agar method for enumeration of microorganisms in various foods.
제언 - 농산물(식품) 오염원에 대한 인식, 올바른가? - 화학적 위해보다 '생물학적.물리적' 위해 중점 관리해야
하상도,Ha, Sang-Do 한국작물보호협회 2010 자연과 농업 Vol.256 No.-
질병 조사에 따르면 일반적 식품오염 유형의 90%가 세균이고, 나머지 6%가 바이러스, 3%가 화학물질이다. 식품 오염원중 생물학적 위해가 주로 질병과 관련이 된다는 이야기다. 소비자는 농식품의 오염원과 위해요소에 대한 올바른 인식을 가져야 할 것이다.
하상도,이성희,류경,김용수,김형일,최현철,전대훈,이영자,이유시 한국식품위생안전성학회 2007 한국식품위생안전성학회지 Vol.22 No.4
. This study surveyed on the actual conditions of using sanitizers and disinfectants for improve-ments of sanitization on food utensils at 105 school and 20 industry foodservice operations. The questionnaire whichwas administered to 125 foodservices was used as a mail or visitation method. The answers of asking Perception ontemporary authorization system of sanitizers and disinfectants on food utensils were 75% in contract managed schoolfoodservices, 81.8% in self operated school foodservices, and 50% in industry. Main factors to choose sanitizers weresterilizing power (38.6%, 28.6%, 38.9%) and safety (32.6%, 46.1%, 33.3%) at every foodservices. Keeping ratio ofsanitizers and disinfectants gui operated school and industry foodserviceswere 64.8%, 52% and 73.7%, respectively. If easy and practical guideline is developed, most foodservices replied touse if for disinfection of foodservices. Most of the foodservices were not only knowing sanitizers and disinfectants butalso possessing a guideline. However, they didnt perform disinfection according to the guideline due to its complex-ity. Consequently, we suggest that it is necessary to provide an easy and practical sanitizers and disinfectants guide-line and useful information.