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한국인에서 5, 10-Methylenetetrahydrafolate Reductase 유전자 다형성에 관한 연구
하권수,최종순,강문수,양동호,홍세용 대한내과학회 1999 대한내과학회지 Vol.56 No.1
Background : Hyperhomocysteinemia has been recognized as an independent risk factor which causes atherosclerosis. The genetic mutation in MTHFR, an enzyme required for efficient homocysteine metabolism, produces a thermolabile enzyme with reduced activity. It has been suggested that thermolabile MTHFR is associated with vascular disease due to increased plasma homocysteine concentration. This study is a preliminary research for identifying the relationship between the MTHFR gene mutation and hypertensive subjects by examining the prevalence of 677 C→T MTHFR gene mutation in the Korean people. Methods and Results : We examined MTHFR gene mutation, BMI, BP, lipid profile of those 312 subjects who visited the health service center of Soonchunhyang University Chunan Hospital from January to March in 1998. The respective prevalence of the homozygous and heterozygous 677 C→T mutation in the MTHFR gene was 51subjects(16%) and 160 subjects(51.1%) and remaining 105 subjects(32.9%) were normal. Hypertensive patients were 83 subjects(26%) and the prevalence of hypertension among each genotypes was similar. (VV 19.6%, AV 26.9%, AA 29.7%) Conclusion : Our data shows that the frequency of MTHFR genotype in the Korean is similar to that of people who live in Asia(Japan), Europe(Italy, Dutch etc), and US Whites, but differ to that of people living in Africa. There is no significant relationship between the MTHFR gene mutation and essential hypertension.
Ha, Kwon-Soo,Leem, Sun-Hee,Choi, Jong-Soon,Kim, Seung Il 東亞大學校附設遺傳工學硏究所 1999 遺傳工學硏究 Vol.- No.6
Previously, we have reported that two clustered cat genes from Acenitobacter lwoffii K24 had different arrangements, catB₁C₁A₁ and catB₂A₂C₂ (Kim, S.I., S.-H. Leem, J.-S. Choi,Y.H.Chung, S.Kim,Y.-M.Park, Y.K.Park, Y.N.Lee, and K.-S.Ha.1997,J.Bacteriol.179,5226-5231). By further analysis of the organization of the cat1 gene ciuster, we obtained a complete sequence of the catB₁ gene, which encoded 40.8-kDa polypeptide containing 379 amino acids, and found a open reading frame (ORF) coding a putative regulatory protein in upstream region of catB₁ on plasmid pCD1-1. This ORF encoded 34.2-kDa polypeptide containing 379 amino acids and had more than 40% identity with catR, LysR family regulatory protein of Pseudomonas putida. RT-PCR, Northern blot analysis and primer extension assay for transcriptional analysis of the cat1 gene cluster revealed that the catB₁C₁ genes were cotranscribed and the catA₁ gene was independently transcribed.
Phospholipase D Is Not Involved in Rho A-Mediated Activation of Stress Fiber Formation
Ha, Kwon-Soo,Kim, Seung-Il,Kim, Jae-Hong,Shin, Incheol,Leem, Sun-Hee,Kweon, Soo-Mi 東亞大學校附設遺傳工學硏究所 1999 遺傳工學硏究 Vol.- No.6
In order to investigate the role of a small GTP-binding protein RhoA in lysophosphatidic acid (LPA)-induced stress fiber formation. C3 ADP-ribosyltransferase was prepared by expressing in E. coli and then applied to Rat-2 fibroblasts. C3 transferase isolated from E.coli was as effective as the toxin from Clostridium botulinum in ADP-ribosylation of RhoA. Incubation of the cells with C3 transferase for 2 days induced ADP-ribosylation of RhoA by a dose-dependent manner, with a sub-maximal induction at 25 ug/ml. As expected, LPA-induced stress fiber formation was completely blocked by pre-incubation with C3 transferase for 2 days. However, exogenously added C3 transferase had no significant effect on the formation of phosphatidylethanol by LPA. These results suggested that phospholipase D was not activated by RhoA in the LPA-induced stress fiber formation.