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하건수,최재근,허남기,김상수,이안수,장진선,윤홍태 한국육종학회 2013 한국육종학회지 Vol.45 No.2
‘호반’은 대립이며 종자 특성이 우수한 GWS91을 모본으로 하고 Lipoxygenase 2와 3이 결핍된 ‘진품콩’을 부본으로1995년도에 인공교배를 하고 1996년부터 1997년까지 F1-F2양성하였다. 1998년부터 2003년도에 F3 이후 세대를 전개하며 계통육종법으로 선발한 GSL95102-2B-16-5-1-1계통으로계통명은 ‘강원5호’이다. ‘호반’의 주요특성으로는 유한신육형이며 꽃색은 자색이고 엽형은 난형이다. 입형은 편구형이고종피색은 황색, 제색은 황색이며 100립중은 29.0 g으로 대립이다. 개화기는 7월 23일, 성숙기는 10월 5일로 ‘진품콩 2호’와 유사한 중만생종이다. 종실 성분중 조단백함량은 39.0%로‘진품콩 2호’와 유사하였고, 조지방 함량은 18.5%로 다소 높았다. 기능성 성분중 아이소플라본 함량이 1,995 ㎍/g 로‘진품콩 2호’보다 25% 높았으며, 콩 비린내 유발 효소인 Lipoxygenase 2가 결핍되었다. 조단백 함량은 ‘진품콩 2호’와 유사하였으나, 조지방 함량은 다소 높았고, 메주와 청국장가공 시 발효정도와 풍미가 ‘진품콩 2호’보다 양호하였으며,청국장 수율도 높았다. 수량성은 2004년부터 2006년까지 3년간 실시한 지역적응시험결과 단작지에서 2.69 MT/ha로‘진품콩 2호’ 2.62 MT/ha 대비 3% 증수하였고, 이모작지에서는 2.60 MT/ha로 ‘진품콩 2호’ 2.59 MT/ha와 차이가 없었다. A new soybean cultivar ‘Hoban’ was released by ARES, Gangwon Province in 2007. The Goals of breeding were for large seed size, high yield, lodging tolerance, and resistance to diseases such as soybean mosaic virus (SMV) and bacterial pustule. ‘Hoban’ was derived from the cross of GWS 91, which has yellow seed coat, large seed size and late maturity, and ‘Jinpum’, which lacked two of three lipoxygenase isoenzymes (L-2 and L-3). The preliminary, advanced and regional yield trials for evaluation and selection were carried out from 2001 to 2006. ‘Hoban’ was characterized as lipoxygenase 2 free, large seed. It has a determinate growth habit with purple flower, yellow seed coat, yellow hilum and large seed weight(29.0g per 100 seeds). The maturing date of ‘Hoban’ was Oct. 5. The content of total isoflavone was 1,995㎍/g which was 25% higher than check variety (‘Jinpumkong 2’). The average yield of ‘Hoban’ was 2.69 MT/ha in the regional yield trials (RYT) carried out in two locations of Korea from 2004 to 2006 which was 3 percent higher than the check variety ‘Jinpumkong 2’. SDS-PAGE on grain storage protein shows that ‘Hoban’ was diffcient in lipoxygenase 2.
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하건수,한태진,허남기 한국육종학회 2002 한국육종학회지 Vol.34 No.1
A new black soybean cultivar with gren cotyledon for cooking with rice, Heugcheongkong was developed at the Kang-won Province Agricultural Research Extension Services in 199. Heugcheongkong was selected from Yeongwol local cultivars. Tobred high-quality soybeans, a superior line was selected in 1994, named Kangwon 2. The preliminary, advanced and regional yieldtrials for this cultivar were caried out from 1995 to 1997. It was named Heugcheong which is the first cultivar that has green cot-yledon with black sed coat in Korea. It showed high resistance to necrotic type of soybean mosaic virus. It has god adaptabilityfor coking with rice. It has determinate growth habit, purple flower, tawny pubescence, dark brown pod, and oval leaf shape.Heugcheongkong is higher than check cultivar in total protein content and sugar content. It contained unsaturated faty acid withinthe sed as high as 86.2%, with 48 mg/100 g of lecithin. The average yield of Heugcheongkong was 2.42 t/ha on the regional yieldtrials carried out for three years from 197 to 19 which was 7 percent lower than the check cultivar Geomjeongkong 1.
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품종별 대두 〔Glycine max L.〕 자엽에서의 부정근 형성
하건수,한태진,Ha, Keon-Soo,Han, Tae-Jin 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.1
대두의 자엽 절편을 이용한 부정근 형성과 품종 반응을 비교하였다. 품종별 부정근 형성 양상은 직접적인 부정근 형성 품종과 callus형성이 동시에 이루어지는 품종들로 구분되었으며, callus 형성이 많은 품종에서는 부정근 형성수가 적었다. 자엽 절편에서의 부정근 형성은 절편의 향축면 치상시에만 부정근이 형성되었고, 배축면과 향축면이 동시에 치상될 경우에도 향축면에서만 부정근이 형성되며, 절편의 배축이 존재하였던 방향에서만 부정근이 형성되는 방향성이 존재하였다. 부정근 형성시 자엽 절편 내 탄수화물은 형성초기보다 형성이 가장 많은 유기 4일에 가장 많이 관찰되었으며 ,형성된 부정근의 생장이 이루어지는 7일 이후에는 관찰되지 않았다. The patterns of adventitious root formation from cotyledons for each cultivar of soybeans were compared. The results of adventitious root formation in cultivars are classified as two groups; the first group showed the direct adventitious root formation, and the second group resulted in the callus and adventitious root formation. The cultivars that have much callus formation had less the adventitious root formation. The adventitious root formation in the cotyledonary explants was occured only at the inoculation of adaxial side. When adaxial and abaxial side was inoculated simultaneously, the adventitious roots were formed at the adaxial side. Thus, it suggests that there must be direction to some extent. Starch in the cotyledonary explants were more abundant at the 4 days after induction than at the early stage of the adventitious root formation, but the starch was not observed after 7 days, that the growth stage of adventitious roots.
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하건수,한태진,Ha, Keon-Soo,Han, Tae-Jin 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.1
For the plant regeneration of soybean (Glycine me L. Merr.), the shoot formation rate, optimal medium and tissue conditions were examined using Korean soybean cultivars. Among the parts of seedling, a node that includes one cotyledon showed the highest shoot formation rate among other tissues. Half-strength B5 medium was more efficient than full strength medium. Formation rates of pair shoots (1 to 2 shooting) were higher in the when benzyl adenine was supplemented. The formation rates of multiple shoots, that is, 4 to 5 in shooting, were high when thidiazuron was supplemented. Multiple shoot was de novo formed in cutting side of cotyledonary node. The effective concentration of thidiazuron for shoot induction treatment was 2 mg/L. Among the 27 cultivars, multiple shoot formation rates were high in the 11 cultivars including 'Heugcheongkong, and pair shoot formation rates were high in the 16 cultivars including 'Malikong'. 대두의 재분화 체계를 확립하고자 품종별 shoot 형성률, 적정배지 및 적정조직을 구명하고자 하였다. 유묘의 조직부위별 비교에서는 1개의 자엽을 포함한 절에서 multiple shoot 형성률이 높았다. 기본배지에서는 1/2 B5배지가 효율적이었으며, TDZ 처리시에 multiple shoot 형성률이 높았으며, 처리농도는 2mg/L가 효율적이었다. Shoot 형성시 자엽 네 전분은 배양 1주까지 증가 후 감소하였으며, 수용성 당은 배양 2주까지 증가 후 shoot가 형성되는 배양 4주에는 관찰되지 않았다. 품종 간 비교에서는 품종에 따라 shoot 형성 양상이 구분되어, '흑청콩' 등 11품종에서는 multiple shoot 형성률이 높았고, '만리콩' 등 16품종에서는 pair shoot 형성률이 높았다. 자엽절에서 shoot가 형성되는 양상에 따른 조직화학적 관찰 결과 pair shoot의 경우 내생적으로 잠재한 액아에 의한 것이며, multiple shoot의 경우는 새로이 형성된 shoot로 확인되었다.
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Kinetic and thermodynamic characteristics of crystallization of vancomycin
하건수,김진현 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.9
We investigated the effect of the major process parameters (crystallization temperature and time) on the efficiency of the vancomycin crystallization process and conducted a kinetic and thermodynamic analysis. The most clear and uniform vancomycin crystals with the highest yield (~98%) were obtained at the optimum crystallization temperature (283 K) and time (1,440 min). The electron microscope, SEM, and XRD analyses showed that intact crystalline vancomycin was obtained when using a crystallization temperature of 283, 288, and 293 K. The kinetic analysis results revealed that the Johnson-Mehl-Avrami-Kolmogorov (JMAK) model was suitable with a high value for r2 (>0.9561) and low value for RMSD (<0.0170). Finally, from the thermodynamic analysis the Gibb’s free energy change (ΔG0), entropy change (ΔS0), and enthalpy change (ΔH0) were all negative, indicating that the crystallization process was spontaneous, irreversible, and exothermic.
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