http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
완두 발아시 Polyamine 함량 변화 및 Peroxidase Isozyme 양상
표병식(Byoung Sik Pyo),이영주(Young Ju Lee),강영희(Young Hee Kang) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.4
In germinating pea, contents of endogenous polyamine in the leaf and stem were determined, and protein content, peroxidase activity and pattern of isozymes were examined in the leaf treated with polyamines. During growth of the pea for 14 days in light condition, the polyamines in leaf and stem showed the highest level at the 5th day, and were decreased rapidly at the 7th day, kept almost constant level since then. The putrescine level was relatively higher than those of spermidine and spermine, and cadaverine was also detected. On the other hand, in the leaf treated with supermine (0.01 mM) protein content increased about 250% than that of the control, the peroxidase activity increased more than 100% in spermine of 0.01 mM and 0.1 mM. In treating with putrescine of 0.1 mM the pattern of peroxidase isozyme appeared 4 new cathodic bands (pI 4.8, 5.6, 5.9 and 6.8) compared with the control, the clear cathodic bands (pI 5.6, 5.9, 6.4, and 6.6) were also observed in spermine of 0.1 mM. These results suggest that polyamines were important factor in the differentiation of pea at the early stage of germination.
표병식(Byoung-Sik Pyo),오영준(Young-Jun Oh),박양원(Yang-Won Park) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.6
토양에서 분리한 균주 IJ-3를 포함하는 미생물이 균체외의 두유응고 효소를 분비하였다. 균주 IJ-3는 Bergey' s에 의해 Bacillus속의 균체로 확인되었다. 두유응고 효소는, pH 5.8~6.4 및 55~75℃ 이하에서 커드를 형성하였다. 두유응고 활성에 대한 최적온도는 65~75℃였고, 효소는 50℃ 이하의 범위의 온도에서 안정하였으며, pH 범위 (pH 6~7)에서 효소고유 활성의 60~100%의 안정성을 보였다. 효소의 분자량은 SDS-PAGE에서 28,000으로 추정하였다. Bacillus sp. IJ-3 효소로 커드는 부드러운 조직과 쓴맛이나 콩비린내가 없는 순수맛을 보였다. Microorganisms, including the strain IJ-3 isolated from soil, were found to secrete an extracellular soybean protein coagulating enzyme and the strain, IJ-3, was identified as Genus Bacillus according to the Bergey's manual. The enzyme coagulated protein in soymilk, thus forming a curd at pHs 5.8~6.4 and at 55~75℃. The optimum temperature for soybean protein coagulating activity was 65~75℃ and the enzyme was stable at temperature below 50℃ and was found to be stable with about 60~100% of the original activity at a with pH ranges (pH 6~7). The molecular weight of enzyme was estimated to be 28,000 by SDS-PAGE. The curd formed with the enzyme from Bacillus sp. IJ-3 has a smooth texture, and a mild taste without any bitterness or a beany flavor.
당근 현탁배양세포에서 Ca2+ 과 Polyamine 이 Callose 함량에 미치는 영향
표병식(Byoung Sik Pyo),강영희(Young Hee Kang) 한국식물학회 1989 Journal of Plant Biology Vol.32 No.4
The effects of Ca^2+ on polyamines on callose contents of carrot suspension cultured cells were studied. The regeneration process of the cell wall of carrot protoplast observed through the electron microscope. Treatment of the carrot suspension cultured cells with Ca^2+ and polyamines resulted in considerable increase on callose contents at 0.1 mM of Ca^2+ and polyamines, particulary spermidine. Poly-L-lysine and poly-L-ornithine increased about 30% and 100% of callose contents than that of the control respectively, whereas verapamil and flunarizine markedly decreased the callose contents. These effects of Ca^2+ and polyamines may be ascribed to a polycationic nature on the callose contents. Futhermore, Ca^2+ and calmodulin increased the callose contents as Ca^2+ of free ion rather than as Ca^2+ -calmodulin complex. During the cultivation of the protoplast, the regeneration of the cell wall was somewhat observed on the 4th day, however, it was inhibited by verapamil. These results suggested that the promotive action of Ca^2+ and polyamines were manifested in the callose contents and the regeneration of the cell wall.
당근 현탁배양 세포에서 Ca2+ 과 Polyamines 가 Cell Wall 합성에 관여하는 β - Glucan Synthetase 2 활성에 미치는 영향
표병식(Byoung Sik Pyo),강영희(Young Hee Kang) 한국식물학회 1988 Journal of Plant Biology Vol.31 No.2
The effect of Ca^2+ and polyamines on the activity of β-glucan synthetase Ⅱ(GSⅡ) related to cell wall synthesis was studied in carrot suspension cultured cells. The activity of GS Ⅱ is four times higher than that of β-glucan synthetase Ⅰ in carrot suspension cultured cells and in vitro experiment, the activity of GSⅡ was increased in response to increase in concentration of Ca^2+ and polyamines. When carrot suspension cultured cells were incubated together with Ca^2+ and polyamines. When carrot suspension cultured cells were incubated together with Ca^2+ and polyamines, the GSⅡ activity was high at 0.1mM of Ca^2+ and 1 mM of putrescine. Also, polycationic poly-L-lysine and poly-L-ornithine increased about 50% the GSⅡ activity than that of the control, respectively. These results may imply that Ca^2+ and polyamines were related to the enzyme activity as a polycationic nature. In addition, verapamil as the calcium channel blocker and flunarizine as an antagonist of calcium mechanism in cytoplasm decreased GSⅡ activity ramarkably, Ca^2+ and calmodulin stimulated GSⅡ activity as Ca^2+ of free ion rather than Ca^2+ calmodulin complex. The effect of 2,4-D on the GSⅡ activity in culture medum is shown to be low at 0.1㎎ per liter and GSⅡ activity increased about 30% more than that of the 0.1㎎/l at the range of 0.3-1.0㎎ per litere. Cummulative results suggest that Ca^2+ and polyfamines stimulate the cell wall synthesis by means of the enhancement of GSⅡ activity responsible for synthesizing the cell wall components.