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Biological Characterization of Epigallocatechin Gallate Complex with Different Steviol Glucosides
탄한,Nahyun M. Kim,염수청,한송희,곽소형,김성보,박준성,목일균,김도만 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.5
Steviol glucosides (SGs) such as rubusoside (Ru), stevioside (Ste), rebaudioside A (RebA) and stevioside glucosides (SG) are herbal tea sweeteners that enhance the solubility and stability of a number of pharmaceutically important compounds. The complex of epigallocatechin gallate (EGCG) with 10% (w/v) each Ru, Ste, RebA or SG enhanced the water solubility of EGCG over 15 times to 345, 312, 341, or 320 mg/mL, respectively. The 2,2- diphenyl-1-picrylhydrazyl radical scavenging (SC50) activities of EGCG, EGCG-Ru, EGCG-Ste, EGCG-RebA, and EGCGSG in water were 5.88, 6.03, 6.52, 4.89, and 4.23 μg/mL, respectively. EGCGs complexed with different SGs maintained inhibitory activities against human intestinal maltase, human pancreatic α-amylase, and the growth of Streptococcus mutans, Helicobacter pylori, Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Clostridium difficile. In glucose tolerance test using C57BL/6 mice, plasma glucose levels in mice treated with EGCG or EGCG-Ste complex were decreased by 9.34%, which was 31.08% lower than those treated with maltose. The efficient and cost-effective EGCG-SGs production method might be applicable to produce water soluble bioactive nutraceuticals in large scale.
Displacement Estimation using MEMS Accelerometer for Low Frequency Motion
탄시영,박수홍,Young, Tan She,Park, Soo-Hong Korea Institute of Electronic Communication Scienc 2013 한국전자통신학회 논문지 Vol.8 No.6
MEMS 가속도계는 운동측정을 위하여 가속도신호를 해석하는 저비용의 방안이다. 이론적으로 변위는 가속도신호를 두 번 적분함으로서 계산될 수 있으나 실제적으로는 실제 존재하는 여러 가지 원인에 의해서 이 방법으로는 계산 될 수가 없다. 본 실험은 실제적인 가속도신호를 획득하여 저주파 운동해석을 위하여 처리하는 과정에 대하여 보여준다. MEMS accelerometer provides a low cost alternative in retrieving the acceleration signal for motion sensing. Theoretically, displacement can be calculated by double integrate the acceleration signal. However, in practical, it could not be done in straight forward manner due to several factors that exist in the real world. This experiment focuses on the processes from practical accelerometer signal acquisition till the result of double integration for low frequency vertical motion.
Synthesis of Oligosaccharide-containing Orange Juice Using Glucansucrase
탄한,서예슬,조재영,이선,Ghahyun J. Kim,윤종원,안승현,황경환,박준승,장태수,김도만 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3
Orange juice is a well-accepted fruit juice, and is a natural source of various vitamins, especially vitamin C, as well as sugar, potassium, thiamine, folate, flavonoids and antioxidants. The respective fructose, glucose, and sucrose concentrations were 9.3, 22.9, and 48.1 g/L in the original orange juice used in this study, and 183.4, 170.1, and 142.8 g/L after concentration. Over 97% of the sucrose in the juice was enzymatically converted to glucooligosaccharides upon addition of 3 U/mL dextransucrase, prepared from Leuconostoc mesenteroides 512FMCM, at 16oC. The synthesized oligosaccharides comprised 35.0% of the total saccharides in the concentrated juice and 31.7% in the original juice. The optimum conditions for oligosaccharide synthesis using the concentrated juice were 35.2 × 10−1 U/mL dextransucrase and 1% Ca(OH)2. The calories in the original and modified concentrated orange juices were 325.4 and 246.7 kcal/L, respectively. Compared to the original concentrated juice, the enzyme-modified concentrated juice prevented the formation of 62.7% of the insoluble glucan resulting from addition of mutansucrase, produced by Streptococcus mutans.
탄비어 아마드,이일수 한국물리학회 2009 새물리 Vol.58 No.2
A superparamagnetic transition in the contrast agent Feridex was observed in the zero-field cooling/field cooling (ZFC/FC) and hysteresis curves. Using the ZFC curve, we could accurately determine the superparamagnetic transition temperature, which is called the blocking temperature. The blocking temperature of the diluted sample is shifted to a temperature lower than that of the undiluted sample. We could observe this phenomenon with a twelve-times diluted sample. We also observed a nonzero coercivity below the blocking temperature, but the coercivity was zero above the blocking temperature. T2 조영제 Feridex에서 보이는 초상자성 전이를 ZFC/FC (Zero Field Cooling/Field Cooling)곡선과 자기이력곡선을 통해 관찰하였다. 초상자성 전이온도인 blocking온도를 ZFC 곡선에서 결정할 수 있었다. 희석된 시료에서의 초상자성전이온도는 쌍극자-쌍극자 상호작용이 줄어들기 때문에 희석되지 않은 시료의 전이온도보다 낮은 온도로 이동한다. 이를 12배 희석된 시료를 사용하여 확인하였다. Blocking 온도이하인 4.2 K에서 자기 이력곡선을 통해 보자력이 존재함을 알 수 있었고 초상자성 온도이상의 온도인 300 K에서는 보자력이 없어짐을 확인하였다.