Effect of Essential Fatty Acid Deficiency on Myelination of the Developing Brain

최혜미,이양자,박현서,Choi, Hay-mie,Lee, Yang-Cha,Park, Hyun-Suh Korean Society for Biochemistry and Molecular Biol 1987 한국생화학회지 Vol.20 No.1

임신한 흰쥐에게 필수지방산이 풍부한 옥수수기름과 필수지방산이 결핍된 야자경화유를 각각 임신 일주일부터 먹여 출생과 이유후 7주까지 사육하면서 적당한 시기에 새끼쥐의 뇌에서 DNA, 단백질, 콜레스테롤, 인지질, 중성지질, Poteolipid protein, 및 지방산 조성을 측정하였다. 필수지방산이 결핍된 새끼쥐는 실험기간동안 체중이 유의적으로 감소했으며 대조군의 약 70% 정도 밖에 되지 않았으나 뇌무게는 별 차이가 없었다. 뇌의 핵산과 단백질은 결핍식이에 영향을 받지않으나 myelin 의 지표인 proteolipid protein은 감소하였다. 뇌지질 또한 결핍식이에 영향을 받지 않았다. 지방산 조성은 양에는 별 이가 없었으나 n-6/n-3의 비는 실험기간동안 일률적으로 낮은 것으로 보아 필수지방산의 결핍증을 알기 위한 좋은 지표라고 생각된다. Sprague Dawley pregnant rats were fed the corn-oil as a EFA-sufficient diet and hydrogenated coconut oil as a EFA-deficient diet during the 14 days of gestation and through lactation. Body and brain weights were measured on pups and the brains were analyzed for DNA, protein, proteolipid protein, cholesterol, phospholipid, and triglyceride. Body weights were significantly low in EFA-deficient group through the 7 week period, but brain weights were significantly depressed before weaning. Brain DNA and protein contents were not affected by EFA deficiency. However, proteolipid protein was significantly lower in the EFA-deficient group than the EFA-sufficient group. Brain cholesterol, phospholipid, and triglyceride were not affected by the EFA deficiency. Fatty acid compositions in the brain were not significantly different between groups but the ratio of n-6/n-3, which is considered to be a biochemical index of EFA deficiency, was much lower in the EFA-deficient pups all through the 7 week period.

논문편 : 전산프로그램을 이용한 경제적 식품구입비 산출 및 식생활관리에의 이용연구

최혜미,김현덕,지선경,곽충실,김현아 ( Hay Mie Choi,Hyeon Duck Kim,Sun Kyung Ji,Choong Sil Kwag,Hyeon A Kim ) 대한가정학회 1991 Family and Environment Research Vol.29 No.3

필수지방산의 결핍이 흰쥐의 뇌발달에 미치는 영향

최혜미,이양자,박현서 ( Hay Mie Choi,Yang Cha Lee,Hyun Suh Park ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.1

Sprague Dawley pregnant rats were fed the corn-oil as a EFA-sufficient diet and hydrogenated coconut oil as a EFA-deficient diet during the 14 days of gestation and through lactation. Body and brain weights were measured on pups and the brains were analyzed for DNA, protein, proteolipid protein, cholesterol, phospholipid, and triglyceride. Body weights were significantly low in EFA-deficient group through the 7 week period, but brain weights were significantly depressed before weaning. Brain DNA and protein contents were not affected by EFA deficiency. However, proteolipid protein was significantly lower in the EFA-deficient group than the EFA-sufficient group. Brain cholesterol, phospholipid, and triglyceride were not affected by the EFA deficiency. Fatty acid compositions in the brain were not significantly different between groups but the ratio of n-6/n-3, which is considered to be a biochemical index of EFA deficiency, was much lower in the EFA-deficient pups all through the 7 week period.

A Study on The Phenolic Content of Potatoes

Hay-mie Choi(최혜미) 한국식품과학회 1976 한국식품과학회지 Vol.8 No.2

Effect of Dietary Lipid on Monoamine Oxidase Activity of Developing Rat Brain

여의주,최혜미,최명언,Yeo, Eui-Zu,Choi, Hay-Mie,Choi, Myung-Un 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.2

식이 지방함량에 의한 성장기 뇌의 monamine oxidase(MAO) 활성도에 미치는 영향을 살펴보기 위해 옥수수 기름을 각각 10, 2, 0.5% 포함한 3종류의 식이를 임신한 Sprague Dawley종의 흰쥐에게 임신말기부터 제공했다. 수유기간중의 영향을 알아보기 위해 대조군(10% 옥수수 기름) 일부에 분만후 0.5% 결핍 식이를 섭취시켰으며, 또 0.5% 식이군의 일부에 이유후부터 10% 정상식이를 주어 회복 효과를 관찰하였다. 전 실험기간동안 임신 말기부터의 결핍 식이군의 MAO활성도는 대조군에 비해 유의적으로 감소했으나 분만후부터 0.5% 결핍식이를 제공한 군에서는 차이가 없었다. 그러나 serotonin을 기질로 하여 MAO type A 활성도를 살펴본 결과 분만후의 결핍에 의해서도 대조군과 유의적인 차이를 보여 주었다. 각 군의 뇌 mitochondria의 지질조성을 살펴본 결과 결핍군에서 대조군에 비해 phospholipids/cholesterol의 비가 유의적으로 낮았고 또한 membrane fluidity의 한 지표인 p/s의 비도 유의적으로 낮았다. 이들로부터 성장 초기에 지방함량을 조절한 식이에 의해 뇌 mitochondria의 지질 조성이 크게 변함을 관찰 할 수 있었고 이에 따라 그 외막에 결합된 MAO 활성도도 유의적인 영향을 받고 있음을 알 수 있었다. The effect of dietary lipid on monoamine oxidase (MAO) activity was examined by feeding pregnant Sprague Dawley rats with three kinds of experimental diets containing 10, 2, and 0.5% of corn oil. A part of control group rats was fed 0.5% deficient diet at birth in order to verify the lipid effect during the lactation period. Furthermore, a part of 0.5% diet groups was rehabilitated after weaning with the control diet for comparison. Throughout the experimental periods, MAO activities of the deficient groups were significantly lower than that of the control group except the MAO activity of the group fed 0.5% diet at brith (D-0.5B). When serotonin was used as substrate, however, the MAO-A activities of the D-0.5B group rats were also significantly lower than that of the control. The results of lipid compositions of each diet groups revealed that the phospholipid/cholesterol ratio of total lipid extracted from brain mitochondria showed significant differences between the control and the deficient groups. Likewise, the p/s ratio of deficient groups were also significantly lower than that of the control group. These observations, therefore, provide an evidence that the alteration of dietary lipid can affect the lipid composition of mitochondria and in consequence the dietary lipid influenced the activity of the membrane-bound MAO.

쥐의 tyrosine결핍이 갑상선호르몬 함량에 미치는 효과

이기옥,최혜미,Lee, Ki-Ock,Choi, Hay-Mie 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.4

Pregnant Sprague Dawley rats were fed a tyrosine deficient diet (tyrosine 0.00%): Def. I group started from delivery, Def. II from the 8th day of gestation, and compared with the control (tyrosine 0.36%) until the 5th week of postnatal period. Offspring rats from these groups were analyzed for serum $T_3$ and $T_$. The serum $T_3$ and $T_4$ levels of def. I group were higher than those of control. The serum $T_4$ level of Def. II group were similar to that of the control at 2nd and 3rd week of postnatal period, and then it was rapidly increased to surpass to control level. However, dietary tyrosine deficiency significantly reduced serum tyrosine level at all ages. When considering overall developmental pattern, the serum $T_3$ and $T_4$ levels of all groups increased and serum tyrosine levels increased transiently at 2nd week and then decreased.

Tyrosine 결핍이 뇌 성장 및 Brain Catecholamine 수준에 미치는 영향

송은승,최혜미,임경숙 ( Eun Seung Song,Hay Mie Choi,Kyeong Sook Yim ) 생화학분자생물학회 1982 BMB Reports Vol.15 No.3

Virgin female Sprague-Dawley rats, weighing 200∼230 g, were mated with healthy males. And pregnant rats were seperated and fed a tyrosine deficient diet (tyr. 0.00 %) during various stages of gestation and lactation period, and the offsprings were compared with the rats fed a tyrosine control diet (tyr. 0. 36%). Brain and serum protein, brain and serum tyrosine and brain catecholamines; norepinephrine and dopamine were measured. Body weight and brain weight were also measured. DEF. I group received the deficient diet from delivery; DEF. II group, from the 15th day of gestation; DEF. III group, from the 8th day of gestation and continued until 35th day of postnatal period. Control group was fed a control diet (tyr. 0.36%) throughout the experimental period. Body and brain weight were significantly lower in deficient groups than those of the control group, but the brain weight was not so severely affected by the tyrosine deficient diet as the body weight. And tyrosine deficiency to have an adverse effect on brain protein synthesis and the adverse effect appeared more severe in preweaning than in postweaning period. In deficient groups, serum tyrosine showed severely decreasing tendency, but brain tyrosine did show such a sharp decrease. Brain norepinephrine and dopamine contents a decreasing tendency in deficient groups compared with the control group. But only at 7th day, both norepinephrine and dopamine contents were significantly depressed in all three DEF. groups. This shows that tyrosine restriction in maternal diet is not so critical to brain catecholamine content of offsprings except the neonatal rats. It seems that norepinephrine level was more affected by dietary tyrosine restriction than the dopamine level.

Effect of Dietary Lipid on Fatty acid Pattern in Developing Brain Mitochondria in Rats

여의주,최혜미,Yeo, Eui-Ju,Choi, Hay-Mie The Korean Nutrition Society 1985 Journal of Nutrition and Health Vol.35 No.9

식이 중 지방함량이 성장기 뇌의 미토콘드리아 지방산 조성에 미치는 영향을 살펴보기 위해 옥수수기름을 각각 10, 2, 0.5% 포함한 3종류의 식이를 임신한 Sprague Dawley종의 흰쥐에게 임신말기부터 제공했다. 수유기간 중의 영향을 살펴보기 위해 대조군(10%옥수수기름 섭취)의 일부에 분만 후 0.5% 결핍식이를 섭취시켰고, 또 이유후부터 0.5% 결핍식이군의 일부에 10% 정상식이를 주어 회복효과를 관찰하고자 하였다. 전 실험기간 동안 체중과 뇌 무게, 단백질 함량을 측정하였고 뇌에서의 미토콘드리아를 분리하여 지방산 조성을 관찰하였다. 출생후 수유기간 동안에는 대조군에 비해 지방결핍식이군에서 체중과 뇌의 무게가 다소 높았으나 이유 후 부터 점점 감소하여 유의적인 차이를 볼 수 없었고 특히 0.5% 식이군의 체중은 생후 6주와 7주에 유의적인 감소를 보였다. 그러나 체중에 대한 뇌무게와 뇌의 단백질 함량에 있어에는 대조군과 결핍군 사이에 유의적인 차이를 볼 수 없었다. 따라서 지방결핍이 뇌 성장에 미치는 영향은 다른 영양소에 비해 크지 않음을 알 수 있었다. 뇌 미토콘드리아의 지방산 조성은 나이와 식이에 따라 변하였다. 나이를 먹음에 따라 단쇄 포화 지방산은 감소하고 다 불포화 지방산의 함량은 증가하였다. 또한 결핍식이군과 대조군 사이에 뇌 미토콘드리아 지방산조성을 살펴 본 결과 지방 결핍의 좋은 지표가 되고 불포화도, triene 과 tetraene의 비, 그리고 ${\omega}-6$ 계열과 ${\omega}-9$ 계열의 비에 있어서 큰 차이를 볼 수 있었다. 이들로 부터 성장초기에 지방함량을 조절한 식이에 의해 뇌 미토콘드리아 지질 조성이 크게 변하고 이에따라 미토콘드리아의 기능도 유의적인 영향을 받고 있음을 알 수 있었다.

뇌 발달과 뇌의 Phospholipid 및 Cholesterol 의 변화

변기원,최혜미 ( Ki Won Byun,Hay Mie Choi ) 생화학분자생물학회 1982 BMB Reports Vol.15 No.3

A quantitative restriction of maternal diet was given to Sprague-Dawley rats during lactation. Animals were seperated into two groups, control and deficient groups. And 30 g of diet during lactation was given to deficient group. All the offsprings were weaned at 3 weeks of age and fed a diet ad libitum for 4 weeks of rehabilitation. Body and brain weight, brain DNA, protein, phospholipid, cholesterol were determined on offsprings of each group randomly sacrificed at 0, 1, 2, 3, 5 and 7 weeks of age. Body and brain weight of offsprings of deficient group were significantly lower than the control group. Their deficits were the largest at 3 weeks of age. After a recovery period, body and brain weights were almost to normal weight but these values were still lower than the control. Brain DNA, protein, phospholipid and cholesterol content of deficient group was lower than the control group. In spite of nutritional rehabilitation after weaning, their contents were not recovered completely. DNA, protein and lipid syntheses in the brains of deficient group were delayed by malnutrition.

식이중 지방함량이 성장기 쥐 뇌의 Monamine Oxidase 활성도에 미치는 영향

여의주,최혜미,최명언 ( Eui Zu Yeo,Hay Mie Choi,Myung Un Choi ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.2

The effect of dietary lipid on monoamine oxidase (MAO) activity was examined by feeding pregnant Sprague Dawley rats with three kinds of experimental diets containing 10, 2, and 0.5% of corn oil. A part of control group rats was fed 0.5% deficient diet at birth in order to verify the lipid effect during the lactation period. Furthermore, a part of 0.5% diet groups was rehabilitated after weaning with the control diet for comparison. Throughout the experimental periods, MAO activities of the deficient groups were significantly lower than that of the control group except the MAO activity of the group fed 0.5% diet at brith (D-0.5B). When serotonin was used as substrate, however, the MAO-A activities of the D-0.5B group rats were also significantly lower than that of the control. The results of lipid compositions of each diet groups revealed that the phospholipid/cholesterol ratio of total lipid extracted from brain mitochondria showed significant differences between the control and the deficient groups. Likewise, the p/s ratio of deficient groups were also significantly lower than that of the control group. These observations, therefore, provide an evidence that the alteration of dietary lipid can affect the lipid composition of mitochondria and in consequence the dietary lipid influenced the activity of the membrane-bound MAO.