석이에서 분리한 GE974의 혈당상승억제효과

최혁재,김동현,김남재,Choi, Hyuck-Jai,Kim, Dong-Hyun,Kim, Nam-Jae 한국생약학회 2000 생약학회지 Vol.31 No.3

GE974, isolated from Gyrophora esculenta, showed significant inhibitory effect on several ${\alpha}-glucosidases$ in vitro in previous study. In the present study, GE974 showed significant inhibitory effect on blood glucose elevation in mice loaded with maltose, sucrose, starch and lactose. Also, it exhibited similar effect in alloxan and streptozotocin induced diabetic mice, and genetic diabetic mice(db/db mice) loaded with maltose in dose dependent manner. These results suggest that GE974 may possess hypoglycemic effect that inhibit competitively ${\alpha}-glucosidase$ activities.

석이에서 분리한 GE974의 ${\alpha}-Glucosidase$ 저해효과

최혁재,김동현,김남재,Choi, Hyuck-Jai,Kim, Dong-Hyun,Kim, Nam-Jae 한국생약학회 2000 생약학회지 Vol.31 No.2

This study was conducted to search for the ${\alpha}-glucosidase$ inhibitor from the natural products. In the previous study, the water extract of Gyrophora esculenta exhibited a potent inhibitory effect on ${\alpha}-glucosidase$ activities. Then, by bioassay-guided fractionation followed by chromatographic separation of the water extract of Gyrophora esculenta, ${\alpha}-glucosidase$ inhibitor was isolated as GE974. GE974 showed significant inhibitory activities on some kinds of ${\alpha}-glucosidase$ in vitro. Its inhibitory mechanism seemed to be competitive for disaccharides. Also, it markedly inhibited ${\alpha}-glucosidases$ of intestine separated from both nondiabetics and diabetics.

마디풀(Polygonum aviculare L.) 성분의 지질과산화억제 및 간보호에 미치는 효과

최혁재,김종우,홍남두,김남재,Choi, Hyuck-Jai,Kim, Jong-Woo,Hong, Nam-Doo,Kim, Nam-Jae 한국생약학회 1997 생약학회지 Vol.28 No.3

The ethylacetate fraction of the overground portion of Polygonum aviculare L. exhibited the anti-lipid peroxidation and the liver protective effect in intoxicated rats. Through silica gel chromatography of the ethylacetate fraction monitered by bioassay, two flavonoids, avicularin and juglanin were isolated as active components. Avicularin and juglanin remarkablely inhibited the lipid peroxidation of rat liver induced by 50% ethanol. Especially avicularin exhibited the stronger anti-lipid peroxidation effect than juglanin. Avicularin as a main principle of Polygonum aviculare L. significantly exhibited liver protective activities by decreasing s-GOT and s-LDH levels which represent for the hepatotoxicity induced by $CCl_4$ in rats. In addition, avicularin significantly decreased not only s-LDH but also s-bilirubin levels in intoxicated rat induced by ${\alpha}-naphthylisothiocyanate\;(ANIT)$. These results suggest that avicularin has the protective effects against the hepatoxicity induced by $CCl_4$ and ANIT in rats.

프로롤리스 물추출물의 약효연구

최혁재(Hyuck Jai Choi),심상범(Sang Bum Shim),김남재(Nam Jae Kim),김종우(Jong Woo Kim) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.3

Propolis is a lipophilic, natural product prepared by mixing the exudates collected from various plants by honeybees with beeswax for the purpose of using to seal hive walls and to strengthen the borders of combs. Because of its versatile bioactivities, propolis has been attracting many investigators` interest. But the pharmacological studies on propolis has, to date, been exclusively performed for an alcohol extract, there is few information of water extract. Therefore, in this study, we investigated the various effects of water extract of Chinese propolis. The water extract of propolis and its fractions of organic solvents showed strong antioxidative activities, especially ether and ethylacetate fractions, and reduced the lipid peroxidation of rat liver in vitro. Additionally the ether fraction of propolis (10 mg/ml) inhibited the activity of hyaluronidase by 50%. In vivo, the water extract of propolis considerably decreased s-GOT, s-GPT and s-LDH activities which represent for the hepatotoxicity induced by CCl₄4 in rats, and prolonged the MST (Medium sevival tinme) and ILS (Increasing in MST over control) by 18% in mice which inoculated with sarcoma 180 ascites cells. These results suggest that the water extract of propolis has various bioactivities as well as the alcohol extract.

석이중 오르시놀 정량 및 급성독성

최혁재(Hyuck-Jai Choi),김남재(Nam Jae Kim),김동현(Dong-Hyun Kim) 대한약학회 2001 약학회지 Vol.45 No.2

In previous study, Gyrophora esculenta showed siginificantly inhibitory effect on alpha-glucosidase in vitro and blood glucose elevation in vivo. In the isolating process of active substance, orcinol was separated from Gyrophora esculenta. Orcinol is kwown to be toxic, therefore, in this study, it was analyzed by the TLC densitometry method for quantitative determination from Gyrophora esculenta. The average amount of orcinol of Gyrophora esculenta was 0.2%. For the purpose of removing orcinol, the water extract of Gyrophora esculenta was sequentially fractionated by organic solvents, and the acute toxicity of each fraction was assedded in mice. Among them, the LD50 of butanol fraction was 1.19g/kg(p.o.) and the weight increase of mice in that group was somewhat retareded.

추출 방법에 따른 감길탕가미방(甘桔湯加味方)의 항염증(抗炎症), 진통(鎭痛), 해열(解熱), 항산화(抗酸化) 및 Histamine 투과 억제효과

진형준,김남재,최혁재,정희재,정승기,Jin, Hyung-Joon,Kim, Nam-Jae,Choi, Hyuck-Jai,Jung, Hee-Jae,Jung, Sung-Ki 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.3

Objectives : Modified Gamgil-tang is a prescription commonly used for respiratory diseases. This thesis was carried out to check the treatment effects and diversity of drug formulation by comparing extraction method of ethanol and water of modified Gamgil-tang. Methods : All experiments were carried out with water and 50% ethanol extraction for comparison. In vivo experiment, hyaluronidase inhibitory effects and trypsin inhibitory effects were tested to measure the anti-inflammatory effects activity. Scavenging effects of DPPH free radical, xanthine oxidase inhibitory effects and inhibition on TBA-RS formation were experimented to measure anti-oxidative effects. With the in vivo experiment, ICR group mice and SD group rats were used as experimental animals. An anti-inflammatory effects experiment were carried out to measure the action on carrageenin-induced hind paw edema: analgesic effects were measured using writhing syndrome induced by 0.7% acetic acid in mice: antipyretic effect was measured using endotoxin, and inhibitory effects of increase vascular permeability induced by 0.5% histamine were measured. Results : For extraction of glycyrrhizin contents, ethanol extract was extracted 2 times of that of water extract. Anti-inflammatory effects showed high in ethanol extract. Anti-oxidative effects measured high in ethanol extract. No significant result was found in inhibition on TBA-RS formation. Analgesic effects were found to be similar in water and ethanol extract. Antipyretic effects were found to be stronger in water extract. Inhibitory effects of increase vascular permeability induced by 0.5% histamine showed stronger in ethanol extract. Conclusion : By measuring anti-inflammatory effects, analgesic effects, antipyretic effects, anti-oxidative effects, and histamine permeation inhibition effects both in water extract and ethanol extract after adding agents such as Mentha Herba, Gardenias Fructus, and propolis to existing Gamgil-Tang, ethanol extract was found to be more effective in anti-inflammatory effects, analgesic effects, anti-oxidative effects, and histamine permeation inhibition effects. The converse was found for antipyretic effect.

센서의 성능에 영향을 미치는 환경 요소들에 기반한 센서 노드 배치 방법

김대영(Dae-Young Kim),최혁재(Hyuck-Jae Choi),이종언(Jong-Eon Lee),차시호(Si-Ho Cha),강석중(Seok-Joong Kang),조국현(Kuk-Hyun Cho),조민호(Minho Jo) 한국통신학회 2008 韓國通信學會論文誌 Vol.33 No.10B

일반적으로 센서들의 위치는 감시 정찰 센서 네트워크의 적용 범위, 데이터 전송 비용, 그리고 자원 관리에 영향을 미치기 때문에, 최적의 위치에 센서를 배치해야 한다. 그러나 산악 지대나 도심 지역과 같은 실 환경에 센서노드를 배치할 경우, 센서에 영향을 미치는 다양한 지형 및 기후 요소들을 고려하기 어렵다. 따라서 이러한 다양한 환경에 센서를 배치할 경우 감시 영역 측면에서 목표 대상을 효율적으로 탐지할 수 있는 센서 배치 방법이 필요하다. 이를 위해 본 논문에서는 센서 배치와 관련된 다양한 기후와 지형 등의 환경 요소들을 분석하고, 이들 영향 요소들이 센서 노드 배치 시 활용될 수 있도록 정량화한다. 이를 바탕으로 센서 탐지 영역 측면에서 정량화된 영향 요소들을 고려함으로써 보다 실효적인 센서 배치 방법론을 제안한다. 또한 본 논문에서 제안한 센서 배치 방법론을 따르는 센서 배치 관리 시스템을 구현함으로써 제안된 방법론의 타당성을 입증한다. The position of sensors generally affects coverage, communication costs, and resource management of surveillance sensor networks. Thus we are required to place a sensor in the best location. However, it is difficult to consider that terrain and climate factors influencing sensors when sensor nodes are deployed in the real world, such as a mountain area or a downtown area. We therefore require a sensor deployment method for detecting effectively targets of interest in terms of surveillance area coverage in such environment. Thus in this paper, we analyze various environmental factors related to sensor deployment, and quantify these factors to use when we deploy sensors. By considering these quantified factors, we propose a practical and effective method for deploying sensors in terms of sensing coverage. We also demonstrate the propriety of the proposed method through implementing a sensor deployment management system according to the method.

온백원이 암세포에 미치는 항암활성 효과

이지영,오혜경,류한성,김남재,정원용,오현아,최혁재,윤성우,류봉하,Lee, Jee Young,Oh, Hye Kyung,Ryu, Han Sung,Kim, Nam Jae,Jung, Won-Yong,Oh, Hyun-A,Choi, Hyuck Jai,Yoon, Seong Woo,Ryu, Bong-Ha 대한암한의학회 2015 大韓癌韓醫學會誌 Vol.20 No.2

Objective : The objective of this study was to investigate the experimental efficacy of anti-tumor activity of the complexed herbal formula, Onbaekwon (OBW), which was derived from the literature of Traditional Korean Medicine, Dongeuibogam. Methods : Nine Cancer cell lines, LoVo, MCF-7, HepG2, AGS, A549, NCI-H69, HL-60, Sarcoma 180, LL/2, were prepared and the cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-dephenyl tetrazolium bromide (MTT) assay. Four of them, NCI-H69, HL-60, Sarcoma 180, and LL/2, showed strong cytotoxic activities and they were additionally undergone flow cytometry to find out their effects on apoptosis. ICR male mice were implanted with Sarcoma 180 intraperitoneally and divided into 8 species for each group. Control group was treated with normal saline, positive control group was treated with cyclophosphamide 8mg/kg, and experimental group was treated with OBW 1 g/kg. Results : Among 9 cancer cell lines, NCI-H69, HL-60, Sarcoma 180, and LL/2, expressed less than 0.10 mg/ml of $IC_{50}$ under 0.1~1mg/ml of OBW. NCI-H69, HL-60, Sarcoma 180, and LL/2, showed dose-dependent efficacy of apoptosis. When Sarcoma 180 cancer cell was implanted in ICR male mice and treated with the OBW, they prolonged the median overall survival for 0.8 days, from 17.5 to 18.3. Conclusion : OBW showed strong cytotoxicity to some cancer cells, which are NCI-H69, HL-60, Sarcoma 180, and LL/2, and its apoptotic activity was dose-dependent. OBW prolonged the median survival of mice implanted with Sarcoma 180. Further researches would be expected to support the efficacy of OBW.

해암단이 수종의 암세포에 미치는 항암 효과

이지영,오혜경,류한성,김남재,정원용,오현아,최혁재,윤성우,류봉하,Lee, Jee Young,Oh, Hye Kyung,Ryu, Han Sung,Kim, Nam Jae,Jung, Won-Yong,Oh, Hyun-A,Choi, Hyuck Jai,Yoon, Seong Woo,Ryu, Bong-Ha 대한암한의학회 2015 大韓癌韓醫學會誌 Vol.20 No.2

Objective : The objective of this study was to investigate the anti-tumor activity of the complexed herbal formula, Haeamdan (HAD). Methods : Seven Cancer cell lines, LoVo, MCF-7, AGS, Sarcoma 180, HL-60, NCI-H69, LL/2, were prepared and the cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-dephenyl tetrazolium bromide (MTT) assay. HAD was applied with various concentrations from 0.1 to 1.0 mg/ml to figure out the appropriate dosage. ICR male mice were intraperitoneally implanted with Sarcoma 180 and divided into 8 species for each group. Control group was treated with normal saline, positive control group was treated with cyclophosphamide 8mg/kg, and experimental group was treated with HAD 1g/kg. Results : Among seven cancer cell lines, HAD exhibited strong cytotoxic activities to followed four cancer cell lines, that is, Sarcoma 180, HL-60, NCI-H69, and LL/2. These cytotoxic activity was expressed under 0.50 mg/ml of IC50 under 0.1~1mg/ml of OBW. When Sarcoma 180 cancer cell was implanted in ICR male mice and treated with the HAD, HAD prolonged the median overall survival for 3.6 days, from 17.5 to 21.1 days. Conclusion : HAD showed strong cytotoxicity to the cancer cells, Sarcoma 180, HL-60, NCI-H69, on in vitro study and it showed anti-tumor activity in vivo with the peritoneal cancer mice by prolonging the median survival for 3.6 days. Further researches would be expected to support the anti-tumor efficacy of HAD.

경희 동서약학연구소 업적 초록집 ( 1984 ~ 1993 ) - 제 9 권 경희동서약학연구소보 ( 1992 ) / 원보 : Mercuric Chloride 에 의한 신장독성 발현 기전 및 억제제 개발에 대한 연구

정세영(Se Young Choung),공재명(Jae Myeong Kong),최혁재(Hyuck Jai Choi),정지창(Jee Chang Jung),노영수(Young Soo Nho) 경희대학교 동서약학연구소 1994 경희동서약학연구소보 Vol.11 No.-