참깨$\cdot$시들음병균(Fusarium oxysporun f. vasinfectum)의 배양여액이 기주식물 (참깨, 복화)과 비기주식물(밀, 벼)의 종자 발아에 미치는 영향

최장열,CHOI Chang Yoel 한국응용곤충학회 1962 한국식물보호학회지 Vol.1 No.-

(1) 위조독소(Fusaric acid)를 생성하는 참깨, 시들음병균(Fusarium oxysporun f. vasinfectum)의 배양여액이 참깨, 밀, 목화, 벼 등의 종자의 발아에 미치는 영향을 구명하기 위하여 본시험에 착수하였다. (2) 공시균의 10종의 계통의 배양여액을 첨가한 발아상에 있어서의 참깨, 목화, 밀, 벼 등의 종자의 발아상태를 조사한 결과 공시균의 몇 가지 계통의 배양여액은 이들 작물종자의 발아에 뚜렷한 경향을 주지 않지만 대부분의 계통의 배양여액은 참깨, 목화, 밀, 벼 등의 종자발아를 완전히 조지하거나 혹은 발아지연 또는발아율의 저하를 초래한다는 것을 알았다. a. 공시균의 10종의 계통중 201호균의 배양여액은 참깨, 목화, 밀, 벼 등의 종자발아를 거의 완전히 저지하고 있으며 발아를 유제하는 독성에 있어서 작물간차이는 볼 수 없었으나 이 밖의 281, 321호균 등의 배양여액은 참깨, 목화. 밀, 벼 등 종자의 발아를 억제하는 독성에 있어서 작물간 차이를 볼 수 있었다. b. Fusarium oxysporum f. vasinfectum의 첩양여액이 종자발아를 억제하는 독성에 대하여 참깨가 가장 감수성이 강하고 벼는 감수성이 가장 약하며 밀, 목화 등의 감수성은 참깨와 혀의 그것의 중간에 속한다. (3) Fusarium oxysporum f. vasinfectum 여러 가지 계통균이 혼재하는 토양에서는 밀, 벼 등 비기주작물도 이 균이 토양중에 생성하는 Fusaric acid에 의하여 종자발아나 발아후의 생육이 저해될 가능성이 있다. 1) The purpcse of the present study is to investigate the effects of culture filtrates of Fuarsium oxysporum f. vasinfectum which is known to produce wilt toxin (fusaric acid) on the germination of host plants (sesame, cotton) and non-host plants (wheat, rice). 2) The experiment on the germination of sesame, cotton, wheat and rice seeds in the seed beds separately added with culture filtr ates of 10 differential strains of Fusarium oxysporom f. vasinfectum demonstrated that culture filtrates of most strains of the fungus inhibit or retard the germination of seeds of 4 plants used in this study while those of a few strains do not give notable influence on the germination of seeds of those plants. a) Culture filtrates of strain 201 of the fungus strongly inhibited the germination of seeds of those plants in nearly same degree, but culture filtrates of the other strains, 281, 321, etc., showed remarkable differences in the toxicity inhibiting or retarding the germination of the seeds of those plants. b) In general, sesame seeds are greatly susceptible, wheat and cotton seeds are moderately susceptible and rice seeds are resistant to the toxicity of culture filtrates of the fungus. 3) In the soil containing a number of differential strains of Fusarium oxysporum f. vasinfectum, the germination of seeds and also the growth of seedlings of non-host plants are possibly checked by the toxic substance, fusaric acid produced by the fungus.

Comparative Analysis of the Antioxidant Activities of Green Peppers Cultivated under Conventional and Environmental-Friendly Farming conditions

최장열,최여진,이성진 한국환경농학회 2010 한국환경농학회지 Vol.29 No.1

Environmental-friendly agriculture (EFA) is defined as the cultivation of crops with reduced amounts or without chemical-synthetic pesticides. Recently, the use of chemical pesticides has decreased significantly; therefore, we cultivated peppers following EFA- and conventional methods and compared their antioxidant activities. To accomplish this, the environmental-friendly cultivated peppers (EFPE) and conventionally cultivated peppers (CCPE) were extracted with 70% methanol and the effects of the extracts on the cell viability, intracellular ROS generation, lipid peroxidation and catalase activity of HepG2 cells were evaluated. EFPE showed a stronger protective effect against oxidative stress induced-cell death than that of CCPE. EFPE also reduced intracellular ROS generation (42.7% to 26.4%) following treatment with hydrogen peroxide more effectively than that of CCPE (24.2% to 6.3%). Furthermore, EFPE and CCPE showed protective effects against lipid peroxidation and induced catalase activity, although these effects were not statistically significant. Taken together, these results suggest that EFPE showed stronger antioxidant activities than CCPE, and thus represent evidence that EFA with biocontrol materials may improve the functional properties of crops and/or secondary metabolites with antioxidant activities when compared with conventional agricultural practices.

분리 트랜잭션 버스 기반 다중프로세서 시스템의 인터럽트 제어 및 동기화

최장열 강원대학교 정보통신연구소 1997 정보통신논문지 Vol.1 No.-

This paper presents the control module of the interrupt of the interrupt bus which supports asynchronous data transfers, interprocessor communications, and reliable interrupt synchronization for a split transaction bus based multiprocessors system. The multiprocessor interrupt control module is composed of interrupt requester, interrupt handler, and bus interface including bus arbitration, and supports direct interrupt, arbitration interrupt, and four interrupt vectors. The tight timing restriction on the interrupt receiving logic is relaxed by the state machine using both the interrupt data and the interrupt synchronization signal which are latched on the interrupt bus buffer. The interrupt bus malfunction due to the signal noise or the fault of a function module is avoided by keeping the operation procedure of each interrupt requester identical.

miR-4708의 multidrug resistance protein-1 발현 조절을 통한 paclitaxel 민감성 증가

최장열,유지원,안상건 대한구강악안면병리학회 2017 대한구강악안면병리학회지 Vol.41 No.2

Multidrug resistance (MDR) remains one of the most significant obstacles in various cancer treatment, and this process often involves dysregulation of the number of micro-RNAs. The aim of this study was to explore the role of miR-4708 on the regulation of MDR-1 expression and the regulation of multidrug resistance (MDR) to chemotherapeutic drugs. Luciferase reporter assays demonstrated that miR-4708 directly binds MDR-1 3’-UTR and down-regulated reporter luciferase activity. The mRNA and protein expression levels of MDR1 were significantly decreased following miR-4708 overexpression. Additionally, the accumulation of rhodamine-123 in paclitacel resistant FaDu cells following miR-4708 transfection was significantly increased compared with control, indicating that the efflux capacity was reduced. These results demonstrated that miR-4708 could be involved in the regulation of MDR via targeting MDR-1 and may provide a potential strategy for reversing drug resistance in oral cancer.

유기농 및 관행 재배 벼 미강의 항산화 활성 분석

최장열,이성진 全南大學校 農業科學技術硏究所 2011 農業科學技術硏究 Vol.46 No.-

To compare antioxidant activities between organic and conventional cultivation of rice bran, three kinds of rice species-Hopum, Hopyeong, and Yungwang-were selected and cultivated in Chonnam province. After cultivation, rice bran was extracted with 70% methanol and analyzed protective activity against oxidative stress induced-cell death and antioxidant activities such as inhibition of intracellular reactive oxygen species (ROS) generation and lipid peroxidation in hepatocellular carcinoma HepG2 cells. When HepG2 cells were treated with three kinds of rice bran extracts for 24 h and followed by the treatment of 1 mM H2O2, organic Hopum and Hopyeong rice bran showed the higher protective activities against oxidative stress induced-cell death than their conventional cultivated rice brans. Yungkwang rice bran did not showed the difference between organic cultivation and conventional cultivation. Organic Hopum, Hopyeong, and Yungkwang rice bran extracts reduced the intracellular ROS generation induced by 1 mM H2O2 more efficiently than their conventional-cultivated rice bran extracts. However, tested rice bran extracts did not show the strong protective effects on lipid peroxidation. From the study, organic Hopum and Hopyeong rice bran extracts showed the higher antioxidant activities than their conventional cultivations and these results indicate that even though phytonutrient contents of rice bran were not measured, significant difference between two cultivation practices were evident.

논문 : 한강 북안 고구려보루의 축조시기와 그 성격

최장열 ( Jang Yeal Choi ) 서울대학교 국사학과 2002 韓國史論 Vol.47 No.-

복강경하 질식 전자궁적출술 (LAVH) 570예에 대한 임상적 고찰

최장열 ( Jang Yeul Choi ),송정인 ( Jung In Song ),황병철 ( Byung Chul Whang ),이순표 ( Soon Pyo Lee ),최유덕 ( Yu Duk Choi ),박찬용 ( Chan Yong Park ),윤성준 ( Seong Jun Yoon ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.10

목적 : 복강경하 질식 전자궁적출술을 시행받은 부인과 환자에 있어서 환자들의 특징과 임상 결과를 보고하여 그 효율성을 평가하기 위하여 후향성 연구를 시행하였다. 연구 방법 : 2001년 9월부터 2004년 2월까지 가천의과대학교 길병원 산부인과에서 골반경하 질식 전자궁적출술을 시행받은 570명의 환자를 대상으로 하였다. 평균 나이, 출산력, 체중, 이전 수술 기왕력, 수술 적응증, 수술 결과, 그리고 수술 합병증 등을 비교하였다. 결과 : 평균 나이는 Objective : To evaluate the clinical outcome and characteristics of laparoscopy assisted vaginal hysterectomy (LAVH) in gynecologic patients. Methods : From September, 2001 to February, 2004, total 570 cases of patients were performed LAVH at Gacheon medi

두계 이병도의 백제사연구 –그 업적과 한계–

崔章烈 충남대학교 백제연구소 2021 百濟硏究 Vol.74 No.-

Yi Byeong-do (courtesy name: Dugye, 1896-1989) was one of the most influential Korean historians of the 20th century, best known as a champion of empirical history. Yi’s lifelong emphasis on empirical methodology is traced back to his years in Tokyo during which he met his Japanese mentor while studying at Waseda University. While he made remarkable achievements throughout the long career as a historian, he was particularly proud of the one he made in the field of ancient Korean history. Yi compiled a volume of research papers he had written on ancient Korean history until his later years, and published the book A Study on the Ancient History of Korea (1976). The book also contained his early research papers on Baekje history, some of which were originally published as early as the Japanese colonial period. His basic idea on Baekje history that the ancient Korean kingdom had two pivotal periods, one when it was under the reign of King Goi and the other under the reign of King Geunchogo, is still widely accepted. A pioneer in the historical research of Baekje, Yi laid the groundwork for this field. Notwithstanding, some of his theories related to Baekje history such as the presumed locations of Jinhan and Wiryeseong Fortress in Hanam or the new date he proposed as the founding year of Mireuksa Temple have been mostly dismissed today. This, however, should not be seen as a proof of imperfection of the Yi’s researches, but rather as a natural consequence of advances in the field. New findings and further research most often lead to an improved understanding of a period. His research based on a modern methodology is considered to have made immeasurable contributions in transforming Korean historiography into historical science and raising scholarly standards in this field. At the same time, Yi has been also criticized as a historian with a colonial view of Korean history. His critics accused him of echoing the Japanese-made colonial vision of Korean history. Such criticism has been mainly directed against his research in ancient history. Yi’s research was undeniably limited by his circumstances as a Korean historian who lived during the colonial period and conformed, in many ways, to the Japanese perspectives and methods. However, the fact that Yi’s research was not free of such limitations in no way diminishes the value of the many contributions he made over his career. A historiographic assessment of a historian ought to be based on a comprehensive and through evaluation of his or her scholarly activities and accomplishments. 한국 사학계에서 가장 영향력이 컸던 두계 이병도(1896~1989)는 일반적으로 실증사학을 대표하는 역사학자로 알려져 있다. 두계가 역사연구 방법론으로 평생 동안 실증적 방법을 중요시한 것은 적어도 일본 와세다대학 유학시절 일본인 스승과의 만남과 연관이 있다고 볼 수 있다. 두계는 긴 생애를 통해 역사가로서 두드러진 족적을 남겼는데, 한국 고대사는 스스로 가장 큰 성과를이룬 분야로 자부하였다. 두계는 만년에 이르기까지 쓴 고대사 논문을 모아『한국고대사연구』(1976) 를 출간하였고, 이 책에 일제강점기부터 개별적으로 발표한 백제사 관련 논문도 재수록하였다. 두계가고이왕대와 근초고왕대를 중요한 획기로 설정한 백제사 체계의 큰 틀은 지금까지도 통설이 되어 유지되고 있다. 백제사 연구는 두계를 제1세대로 하여 그 기반이 이룩되었다고 볼 수 있다. 다만, 두계가 제시한 백제사 관련 여러 견해들, 이를테면 삼한의 위치 비정, 하남위례성 비정, 미륵사 창건연대 재해석 등은 더 이상 인정받지 못하고 있다. 이는 두계의 연구가 부실했기 때문이라기 보다 그간 신자료의 발굴및 관련 연구의 심화 등 학문적 발전에 따른 자연스러운 현상으로 보아야 할 것이다. 두계는 근대적 역사연구 방법에 입각한 연구로 한국사학이 역사학으로 정립하고, 그 학문적 수준을향상시키는데 크게 이바지 하였다고 평가받는다. 한편으로는 식민주의 사학자로 비판을 받기도 한다. 그의 한국사 연구가 일본인들이 만든 식민사관을 추종한다는 것이다. 두계에 대한 이러한 비판은 주로 고대사 분야에 집중되어 왔다. 일제강점기 한국인 연구자의 연구가 내포하고 있던 한계, 즉 일본인들이 설정하고 이끌고 간 문제의식이나 연구방법에서 크게 벗어나지 못하였음을 부인할 수 없다. 두계 역시 여기에서 자유로울 수 없다. 그렇다고 두계가 쌓아왔던 연구업적을 평가 절하할 수는 없다. 역사가에 대한사학사적 평가는 그의 학술 활동과 학문적 업적에 대한 충분한 검토를 바탕으로 이루어져야 할 것이다.

제대정맥내피세포에서 발현되는 열충격단백질90 및 27에 대한 연구

김석영 ( Suk Young Kim ),최장열 ( Chang Yeol Choi ),강혜진 ( Hye Jin Kang ),황유진 ( Yu Jin Hwang ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.5

벼의 원형질체 융합에 관한 연구

한창열(Chang Yawl Harn),최장경(Chang Kyung Choi) 한국식물학회 1972 Journal of Plant Biology Vol.15 No.4

Rice callus was treated with 0.56M sucrose solution mixed with 5% pectinase and 10% cellulase, and the protoplasts isolated were transferred to 0.25M sodium nitrate to induce protoplasmic fusion. Callus tissues were macerated well and degradation of cell walls also proceeded satisfactorily. When the protoplasts were transferred to sodium nitrate solution, many giant roundish protoplasts and some multilobed complex protoplasmic bodies were observed. Most of the fusions took place immediately after the protoplasts were transferred to sodium nitrate. Some multilobed protoplasts which failed to fuse in the initial stage took longer time, about two hours, to get completely fused and rounded-off. Multilobed protoplasmic bodies were invariably multinucleate, while giant round protoplasts had either several nuclei or had one nucleus of large size. Nuclear fusion, also, seemed to occur immediately after the protoplasts were transferred to sodium nitrate.