Identification of genomic regions associated with piglet survival and mortality

최윤정,김상욱,윤준호,Bong-Hwan Choi,Tae-Hun Kim,김관석 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.2

One of the major problems in the modern pig in- dustry is infectious disease. Susceptibility to infec- tious diseases is influenced by both constitutional (e.g. genotype, age, gender, and reproductive status) and environmental factors (e.g. nutrition, management, infections, and other forms of stress). Genetic varia- tions within individual animals or herds can cause dif- ferences in the execution of immune functions against infectious agents in domestic pigs. The objectives of the present study were to identify genetic factor(s) responsible for piglet survival and mortality under commercial field conditions with infectious diseases as well as determine QTL regions for immune capac- ity in a pig reference family. Sex ratio was examined between normal grown and presumed dead groups of F 2 animals generated by Korean native pigs (KNP) and Yorkshire (YS) breeds. The ratio of males was significantly higher in the presumed dead group than in the normal grown group. In order to study genetic factors associated with presumed mortality under dis- ease outbreak, allelic frequencies of 239 single nucleo- tide polymorphisms (SNPs) were compared between normal grown and presumed dead groups. Exactly 22 SNPs showed significant differences in their allelic frequencies between the two groups, and four of them were validated with another commercial population divided by normal and emaciated pigs. Furthermore, the relationships between 239 SNPs and immune- related traits were studied. These results demonstrate that identification of genetic components of animal im- mune systems and susceptibility to infectious diseases is possible and will be useful to improve disease resis- tance in individuals as well as in breeding programs.

요양병원 간호인력의 병원감염관리 지식이 병원감염관리 수행에 미치는 영향: 건강신념의 매개효과

최윤정,Choi, Yun-Jung 한국디지털정책학회 2021 디지털융복합연구 Vol.19 No.7

본 연구는 요양병원 간호인력을 대상으로 병원감염관리 지식과 병원감염관리 수행 간의 건강신념의 매개효과 확인하기 위해 실시되었다. 부산 소재 5개 요양병원에 근무하는 간호사, 간호조무사를 대상으로 연구하였으며, 최종적으로 212부의 설문지를 이용하여 SPSS 25.0, SPSS Procee Macro를 통해 분석하였다. 연구결과, 병원감염관리 지식은 병원감염관리 수행에 유의미하였고(B=2.90, p<.001), 건강신념의 영향력도 유의미하게 나타났다(B=.52, p<.001). 병원감염관리 지식은 병원감염관리 수행에 직접적인 영향을 미치며, 건강신념을 통해서도 간접적으로 병원감염관리 수행에 영향을 미치는 것으로 나타나 부분매개효과를 확인하였다. 본 연구는 요양병원에 근무하는 간호사, 간호조무사가 인지하는 병원감염관리 지식이 병원감염관리 수행에 미치는 영향에 있어 건강신념의 매개효과를 확인함으로써 요양병원 간호조직의 효과적인 병원감염관리를 수행함에 필요한 기초자료를 제공함에 그 의의가 있다. This study was conducted on nursing staff at care hospitals to confirm the mediating effect of health beliefs between the knowledge of nosocomial infections management and the execution of nosocomial infections management. This study was carried out on nurses and assistant nurses working at five care hospitals located in Busan, and finally 212 questionnaires were analyzed through SPSS 25.0 and SPSS Procee Macro. The results of the study showed that knowledge of nosocomial infections management is significant in the execution of nosocomial infections management(B=2.90, p<.001), and the influence of health beliefs was shown to be significant as well(B=.52, p<.001). Knowledge of nosocomial infections management has a direct effect on the execution of nosocomial infections management, and was also shown to have an indirect effect on the execution of nosocomial infections management through health beliefs, confirming partial mediating effects. This study is significant in that it provides the baseline data necessary for the effective execution of nosocomial infections management of nursing organizations in care hospitals by verifying the mediating effects of health beliefs in terms of the effects of knowledge of nosocomial infections management perceived by nurses and assistant nurses working in care hospitals on the execution of nosocomial infections management.

사군자탕(四君子湯) 및 사물탕(四物湯)이 methotrexate로 유도(誘導)된 흰쥐의 면역기능저하(免疫機能低下)에 미치는 영향(影響)

최윤정,김장현,Choi Yun-Jung,Kim Jang-Hyun 대한한방소아과학회 1999 대한한방소아과학회지 Vol.13 No.1

The effects of Sagunjatang and Samultang on the immunosuppression induced by methotrexate(MTX) in rats were investigated in this study. The multiple parameters of immunity assessed in each rats included leukocyte count, lymphocyte rate, the number of lymphocyte in tibial bone marrow, contact hypersensitivity to DNFB, morphological change of thymocyte and IgG antibody on SDS-PAGE. Sprague-Dawley male rats were used and divided into five groups at random. Group A was normal control. Group B, the MTX treatment control, was injected i.v. with 2mg/kg of on days 9, 11 after sensitization with SRBC on 5th day. Group C, the experimental control, was treated Sagunjatang for 18days and MTX. Group D was treated Samultang for 18days and MTX. Group E was treated Sagunjatang-Samultang for 18days and MTX. The dosage of Sagunjatang and Samultang was $1m{\ell}/day$ respectively. In the case of Group E, rats Were fed Sagunjatang $1m{\ell}$ in the morning and Samultang $1m{\ell}$ in the afternoon. The results are summarized as follows: 1. Leukocyte count in rats induced by intravenous sensitization with SRBC was decreased significantly in Group E. 2. Leukocyte counts of 2weeks later after being treated MTX were increased significantly in Groups C and D. 3. Lymphocyte rate in rats induced by intravenous sensitization with SRBC wasn't changed significantly in all the experimental groups. 4. Lymphocyte rate of 2weeks. later after being treated MTX was increased significantly in Group D. 5. The number of lymphocyte in tibial bone marrow was incereased significantly in Group C. 6. Contact hypersensitivity wasn't changed significantly in all the experimental groups. 7. Morphological finding of thymocyte in group C was similar to normal group as compared with control group. 8. Purified IgG of all the experimental groups showed two bands of 50,000 and 25,000 on SDS-PAGE. But there was no difference among experimental groups.

학교교실의 냉방시 실내열.공기환경 실태

최윤정,정연홍,이선아,김혜경,황진아,Choi, Yoon-Jung,Jeong, Youn-Hong,Lee, Seon-A,Kim, Hye-Kyeong,Hwang, Jin-A 한국주거학회 2007 한국주거학회 논문집 Vol.18 No.4

The purpose of this study were to make clear the present condition of indoor thermal and air environment by cooling in school classrooms and to analyze the relation of the living conditions with indoor environment. The measurements on physical elements and observations on living conditions were carried out in 6 classrooms of 3 middle or high schools. Measuring elements were indoor temperature, relative humidity, PM10 and $CO_2$ concentration. As results, the averages of indoor temperature each classrooms were $24.9{\sim}26.6^{\circ}C$. Most of classrooms were lower than the Maintenance standard $(26{\sim}28^{\circ}C)$ of School Health Law. The means of relative humidity were $51.3{\sim}72%$, all classrooms were ranged within the standard $(30{\sim}80%)$. The means of PM10 concentration were $3.5{\sim}23.1{\mu}g/m^3$, all classrooms were kept within the standard $(100{\mu}g/m^3)$. The means of $CO_2$ concentration were $1218.7{\sim}4705.4ppm$, all classrooms were exceed the standard (1,000ppm). The results of analysis on relations of living conditions with the physical elements are as follow; the air conditioner set of temperature, windows and doors opening elapsed time, the number of students in classrooms and activities of students had certain effect on indoor environment.

백내장이 동반된 녹내장 환자에서 수정체유화섬유주절제술과 섬유절제술의 치료효과 비교

최윤정,DouglasJ,Rhee,최규룡,Yun Jeong Choi,Douglas J,Rhee,Kyu-Ryong Choi 대한안과학회 2011 대한안과학회지 Vol.52 No.11

Purpose: To compare the treatment outcome and complications of phacotrabeculectomy and trabeculectomy in patients with glaucoma and cataracts. Methods: The authors of the present study retrospectively reviewed the records of 56 eyes that underwent phacotrabeculectomy and trabeculectomy with mitomycin-C between March 2006 and December 2009 in patients with glaucoma and cataracts. The change of intraocular pressure (IOP) and number of glaucoma medications up to 24 months postoperatively were compared and the treatment failure rate as well as postoperative complications analyzed. Results: IOP and number of glaucoma medications at 24 months after surgery were similar in the 2 groups. Additionally, both groups showed a significant decrease in IOP and number of glaucoma medications after surgery. The cumulative failure rates, frequency and distribution of postoperative complications were also similar in both groups and conjunctival bleb leak was the most common complication. Conclusions: Both phacotrabeculectomy and trabeculectomy showed long-term IOP reduction and a similar failure rate and complications. Therefore, both can be considered as a primary surgical treatment in patients with glaucoma and cataracts. In patients who need strict IOP control, trabeculectomy can be preferentially considered. If patients have visually significant cataracts or greater visual needs, phacotrabeculectomy can be considered. J Korean Ophthalmol Soc 2011;52(11):1308-1317

경계범주 자동탐색에 의한 확장된 학습체계 구성방법

최윤정,지정규,박승수,Choi, Yun-Jeong,Jee, Jeong-Gyu,Park, Seung-Soo 한국정보처리학회 2009 정보처리학회논문지B Vol.16 No.6

본 논문은 기존의 목표항목만을 위주로 한 학습체계에서 발생하는 오분류 문제의 해결을 위해 기존의 학습체계에 경계항목을 자동으로 탐 색하여 포함시켜 확대시키는 방법을 제안하고 있다. 여러 주제에 걸쳐 다양한 내용을 다루는 복잡한 문서들은 확실히 어느 범주로 분류해야 할 지 판가름하기 어려운 성질인 모호성이 강하다. 이러한 경우 모든 경우들을 정확히 구분할 수 있는 최적의 경계를 찾는 일은 더욱 어려운 일이 다. 복잡하고 불확실성이 높은 데이터들의 특징은 대부분 분류 경계영역에 위치하므로 이러한 분류경계의 데이터들을 새로운 학습 항목으로 인 식시키도록 하는 것이 필요하다. 본 연구에서는 주어진 목표항목 사이의 경계항목을 자동으로 탐색하여 학습체계에 추가하는 학습 체계 확장 알고리즘을 제시하고, 의도적인 학습오류를 발생시킨 후 기존방법과의 비교실험을 수행함으로써 제안방법의 정확성과 안정성을 비교하였다. 실 험결과 경계범주를 포함하여 학습 체계를 확장시켰을 때의 예측력은 기존 0.70에서 0.86으로 약 24% 향상 되었고, 오류를 포함시켰을 때의 예 측력은 기존 0.52에서 0.79로 약 49% 향상되었다. This paper shows a reinforced construction scheme of training data for improvement of text classification by automatic search of boundary category. The documents laid on boundary area are usually misclassified as they are including multiple topics and features. which is the main factor that we focus on. In this paper, we propose an automated exploring methodology of optimal boundary category based on previous research. We consider the boundary area among target categories to new category to be required training, which are then added to the target category sementically. In experiments, we applied our method to complex documents by intentionally making errors in training process. The experimental results show that our system has high accuracy and reliability in noisy environment.

고압동결고정을 이용한 애기장대 줄기의 cryo-SEM 분석법

최윤정,이경환,제아름,채희수,장지훈,이은지,권희석,Choi, Yun-Joung,Lee, Kyung-Hwan,Je, A-Reum,Chae, Hee-Su,Jang, Ji-Hoon,Lee, Eun-Ji,Kweon, Hee-Seok 한국현미경학회 2012 Applied microscopy Vol.42 No.2

The scanning electron microscopy is an ideal technique for examining plant surface at high resolution. Most hydrate samples, however, must be fix and dehydrate for observation in the scanning electron microscope. Because the microscopes operate under high vacuum, most specimens, especially biological samples, cannot withstand water removal by the vacuum system without morphological distortion. Cryo-techniques can observe in their original morphology and structure without various artifacts from conventional sample preparation. Rapid cooling is the method of choice for preparing plant samples for scanning electron microscopy in a defined physiological state. As one of cryo-technique, high-pressure freezing allows for fixation of native non-pretreated samples up to $200{\mu}M$ thick and 2 mm wide with minimal or no ice crystal damage for the freezing procedure. In this study, we could design to optimize structural preservation and imaging by comparing cryo-SEM and convention SEM preparation, and observe a fine, well preserved Arabidopsis stem's inner ultrastructure using HPF and cryo-SEM. These results would suggest a useful method of cryo-preparation and cryo-SEM for plant tissues, especially intratubule and vacuole rich structure.

대학교 신축 기숙사의 휘발성유기화합물 농도 및 새집증후군 반응

최윤정,오예슬,장윤정,김정재,Choi, Yoon-Jung,Oh, Ye-Seul,Jang, Yoon-Jeong,Kim, Jung-Jae 한국주거학회 2009 한국주거학회 논문집 Vol.20 No.6

The purposes of this study were to determine the actual state of the indoor air quality by measuring TVOC Concentration and surveying residents' responses in a newly built university dormitory and to analyze the influencing factors of IAQ. Field measurements on the concentrations of TVOC and HCHO were carried out three times at 4 week intervals in 3 rooms of a dormitory. A questionnaire survey was conducted on residents which inquired into the resident's lifestyle, their consciousness of IAQ, and the responses to SHS. According to the results, TVOC concentrations were 0.14~18.5 ppm and HCHO concentrations were 0.23~6.89 ppm during the 3 months following the completion of construction, showing a serious state in which standard levels are exceeded, though these levels seemed to decrease over time. The factors influencing the differences in the levels of TVOC and HCHO were the ventilation volume including infiltration, the heating temperature, relative humidity, or the use of living matter that include chemical ingredients. However, the residents rarely felt the symptoms of SHS and were not conscious of the importance of ventilation.

아파트의 웰빙요소 도입현황과 선호도

최윤정,Choi, Yoon-Jung 한국주거학회 2007 한국주거학회 논문집 Vol.18 No.1

The purposes of this study were to summarize the concept of well-being and well-being apartment, to grasp the present condition of apartments which were introduced with well-being elements, and to find out the consumer preferences on well-being elements for apartment planning. Library and internet surveys were performed to summarize the concept of well-being and well-being apartment and to grasp the present condition of apartments which were introduced with well-being elements. Questionnaire survey was carried out from 2nd to 22nd of June 2005, to investigate the preferences on well-being elements for apartment planning. The respondents were 250 residents who are from thirties to fifties and living in urban area. As results, respondents think that 'living for health of body and mind' about concept of well-being and 'certificated apartments by green building rating system' or 'apartments introduced ecological factor' about concept of well-being apartment. They answered that 'yes' about 'Do you have intention to buy well-being apartment?'. The elements in aspect of complex planning having the preference were revealed that promenade for complex design, ecological garden or walking space for landscape design, outdoor exercise space for outdoor design, and security system for foundation equipment. The elements having the preference in aspect of public facilities were fitness room for sports & health facility and study room for cultural facility. The preferred elements in aspect of building and unit design were roof garden for building design, multi-functional room for unit floor plan, natural surface material for interior surface, ventilation system for indoor environment, control system for home automation, and food waste machine for home electronics.

클라미디아 시타시에 의한 비전형 유두 각결막염 1예

최윤정,전루민,Yun Jeong Choi,Roo Min Jun 대한안과학회 2010 대한안과학회지 Vol.51 No.4

Purpose: Only a few cases of keratoconjunctivitis caused by Chlamydophila psittaci have been reported worldwide, and no case reported in Korea. We report an atypical case of keratoconjunctivitis caused by Chlamydophila psittaci. Case summary: A 34-year-old male patient who had raised a parrot at home presented with three weeks of conjunctival injection and a week of ocular pain in his left eye. There were papillae on the left upper and lower tarsal conjunctiva and punctuate epithelial erosion of the entire cornea. He also complained of dizziness, fever, and dyspnea. Upon chest X-ray, consolidation on the right middle lobe was apparent. The Chlamydophila IgM antibody test was positive, and the pneumonia improved quickly. Nevertheless, signs of keratoconjunctivitis persisted despite 3-week treatment with oral doxycycline. As a result, the patient received an additional 10-day treatment with oral azithromycin. Four weeks after the first visit, symptoms were improving gradually, and, after six weeks, no signs of keratoconjunctivitis remained except minimal erosion. Conclusions: When patients show keratoconjunctivitis after contact with a bird, prolonged ketatoconjunctivitis by Chlamydophila psittaci should be considered. J Korean Ophthalmol Soc 2010;51(4):611-615