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저장성 용액에 노출된 햄스터 난자에 관찰되는 이온전류의 변화
최원영,김양미,한재희,허일오,박춘옥,홍성근,류판동,김종수,Choi, Won-yeong,Kim, Yang-mi,Haan, Jae-hee,Huh, Il-oh,Park, Choon-ok,Hong, Seong-geun,Pyu, Pan-dong,Kim, Jong-shu 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2
Cell volume regulatory mechanisms are usually disclosed by exposure of cell to anisotonic media. If a cell is suddenly exposed to hypotonic media, it swells initially like an osmometer but within minutes regains its original cell volume. This behavior has been labelled as regulatory cell volume decrease(RVD). RVD is believed to result from the loss of permeable ions through the membrane. In this study, we examined hypotonically induced changes in the membrance currents involved in RVD by using whole cell voltage clamp technique in the unfertilized hamster egg. At -40mV of the holding potential, the stationary current was maintained in the hamster egg exposed to isotonic solution composed of, mainly, 115mM NaCl and 40mM mannitol. Hypotonic solution was prepared by removing mannitol. Therefore, the concentrations of $Na^+$ and $Cl^-$ in this hypotonic media were the same as those in the isotonic solution. Following 30 to 60 sec after applying the hypotonic media to the egg, the inward current was evoked. This inward current was eliminated by $100{\mu}M$ 4-acetamido-4'-isothiocyanostil-bene-2,2'-disulfonic acid(SITS), an anion channel blocker, leaving the small outward current component. Further addition of 2mM $Ba^{2+}$, a broad $K^+$ channel blocker, completely abolished the small outward current left even in the presence of SITS during hypotonic stress. These results suggest that $K^+$ and $Cl^-$ move out of cells, resulting in RVD. To test the involvement of $Na^+$ in RVD, 20mM Na-isethionate was substituted for mannitol in isotonic media(135mM $Na^+$) and Na-isethionate (20mM) was freed the hypotonic solution. Only $Cl^-$ concentration in both isotonic and hypotonic media was kept constant at 115mM, whereas concentration of $Na^+$ was lowered in hypotonic solution to 115mM from 135mM in isotonic solution. Hypotonic medium induced the outward current in the egg equilibrated isotonically. This current was reduced by $100{\mu}M$ SITS but was augmented by 2 mM $Ba^{2+}$. In terms of RVD, these results imply that $Cl^-$ efflux is coupled with $K^+$, maybe for electroneutrality during hypotonic stress and/or with $Na^+$ via unknown transport mechanism(s). From the overall results, the hypotonic stress facilitates the movement of $Cl^-$ and $K^+$ out of the hamster egg to regain cellular volume with electroneutrality. If there exist a difference in $[Na^+]_0$ between isotonic and hypotonic solution, another transport mechanism concerned with $Na^+$ may, at least partly, participate in regulatory volume decrease.
최원영,이규성,고종철,문상훈,김정곤,Choi Weon-Young,Lee Kyu-Seong,Ko Jong-Cheol,Moon Sang-Hoon,Kim Chung-Kon 한국작물학회 2005 Korean journal of crop science Vol.50 No.suppl1
본 연구는 남서해안 간척지에서 토양 염농도(저염; $0.1\%$, 중염; $0.3\~0.4\%$)별로 쌀 품질 향상을 위한 완전 낙수시기를 구명하기 위하여 시험한 결과를 요약하면 다음과 같다. 1. 저염토양에서의 완전낙수시기에따라 $m^2$당 립수가 비슷하여 쌀 수량은 유의차가 없었다. 중염토양에서는 완전낙수시기가 빠를수록 염피해를 받아 등숙비율이 낮아져, 쌀 수량은 출수후 30일까지는 감수하였고 35일 이상에서는 같았다. 2. 토양 염농도간의 쌀 수량은 중염 토양에서 저염 토양의 $64\%$ 수준으로 감소하였다. 3. 저염 토양에서 완전미 비율이 높았으나 현미품위는 처리농도간에 차이를 볼 수 없었다. 4. 2002년 기상은 완전낙수시기에 잦은 강우로, 2003년에는 생육기 저온으로 인하여 완전 낙수시기를 결정하기 어려웠지만 수량 및 도복 등을 고려해 볼 때 저염 토양에서는 출수 후 $20\~40$일에, 중염 토양에서는 출수 후 $35\~40$일에 완전낙수하는 것이 안전할 것으로 생각된다. This study was conducted to identify the effective dyainage-time influencing rice yield and grain quality on a reclaimed saline soil, the experiment conducted at the Gyehwado substation of the Honam Agricultural Research Institute from 2002 to 2003. The experimental field contained $0.1\%\;and\;0.3\~0.4\%$ NaCl in soil solution, respectively. The experiment involved salinity levels as main plot and sub-plot where consisted of 6 treatments in a split plot design with three replicates. Rice yield performance showed a maximum at drainage-time for 35 days after flowering under medium salinity level, but indicated only $64\%$ of yield level compare to low soil salinity. Percentage of well-shaped rice was high in low soil salinity indicating there aye severe affection for grain quality in medium soil salinity regardless water drainage-time.
Evaluation of Image qualify in Super-resolution Image Guide for Ultrathin Endoscope
최원영,오창현,이봉수,Choi W.Y.,Oh C.H.,Lee B.S. The Korea Society of Medical and Biological Engine 2000 의공학회지 Vol.21 No.3
In general. fiber-optic medical endoscopes are made from glass step index (SI) fibers. These endoscopes have limitations in both image quality and mechanical properties. In particular. the image resolution of the SI endoscopes is limited to about 5$\mu$m. In this study the image resolution of plastic graded index (GRIN) super-resolution image guides with pixel sizes from 7 to 2.5 $\mu$m were measured and compared with those of 91ass SI image guides. There is an improvement in resolution of the plastic GRIN image guides as the microfiber diameter is reduced from 7 $\mu$m to 2.5 $\mu$m. The measured resolution of plastic GRIN image guide with 2.5 $\mu$m microfibers is more than a factor of two higher than that of g1ass SI image guide with 5$\mu$m microfibers. This new design of optical systems could have a major impact on a wide array of future optical systems used in defense. industrial, and medical applications. 현재 의료용으로 사용되고 있는 광섬유를 이용한 내시경은 굴절률이 다른 두 가지 유리로써(Step Index SI) 제작되고 있다. 이와 같은 내시경은 취급에 주의하여야 하는 근본적인 재료상의 문제점 외에도 영상 가이드의 최고 분해능이 5$\mu$m으로 제약되고 있는 실정이다. 본 연구에서는 플라스틱을 이용하여 고 영상 분해능($7\mu\;m\~2.5\mu$m)을 가진 미세 내시경(Ultrathin Endoscope)용 영상 가이드의 분해능을 측정하였다. 그 각각의 플라스틱 광섬유 굴절률은 광섬유 중심으로부터 외곽까지 점차 감소하는 특성 (Graded Index GRIN)을 가지고 있다. 플라스틱 GRIN 영상가이드의 분해능 측정 결과. 마이크로 광섬유의 지름이 작아질수록 그 분해능이 증가되었고, 유리를 이용한 SI 영상 가이드 분해능과의 비교 연구 결과, 2배의 분해능을 가진 (2.5$\mu$m) 플라스틱 GRIN 영상가이드 제작이 가능함을 보였다. 이와 같은 플라스틱 GRIN 광섬유 다발은 의료 분야뿐 아니라 국방 및 산업분야의 새로운 광학적 설계에 많은 영향을 줄 것으로 기대된다.