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${\varepsilon}$-Caprolactam 이용성(利用性) 세균(細菌)의 분리(分離) 및 그 성질(性質)
최선택,이인구,Choi, Sun Taek,Rhee, In Koo 경북대학교 농업과학기술연구소 1985 慶北大農學誌 Vol.3 No.-
대구(大邱) 신천(新川) 하상(河床) 오니(汚泥)로부터 ${\varepsilon}$-caprolactam이용성(利用性)이 우수한 균(菌)을 분리(分離)하여 Arthrobacter globiformis N-2-1로 동정(同定)했다. Arthrobacter globiformis N-2-1의 배양(培養)을 위한 최적배지(最適培地)의 조성(組成)은 ${\varepsilon}$-caprolactam 0.4%, $K_2HPO_4$ 0.2%, $KH_2PO_4$ 0.05%, $MgSO_4{\cdot}7H_2O$ 0.02%, $F3Cl_3{\cdot}6H_2O$ 0.01%, yeast extract 0.05%이었다. 이 균(菌)의 생육(生育)을 위한 최적(最適) pH는 7.0, 온도(溫度)는 $30^{\circ}C$ 부근(附近)이었다. 본(本) 균(菌)은 무기염 배지(培地)에서 yeast extract를 가(加)하지 않고서도 ${\varepsilon}$-caprolactam을 잘 분해(分解)하여 증식(增殖)할 수 있었으나, yeast extract를 가(加)하면 생육(生育)이 더욱 촉진되었다. 또한 본(本) 균(菌)은 대부분의 당(糖)을 잘 이용(利用)할 수 있었고 유기산(有機酸)으로는 ${\alpha}$-ketoglutarate, adipate, p-hydroxybenzoate는 잘 이용(利用)하였으나 gluconate는 잘 이용(利用)할 수 없었다. 그리고 amino acid는 대부분 잘 이용(利用)하는 것으로 나타났다. 0.4%의 ${\varepsilon}$-caprolactam을 함유한 배지(培地)에서 60시간(時間) 배양(培養)으로 ${\varepsilon}$-caprolactam을 완전히 분해(分解)할 수 있었다. 분리균(分離菌) N-2-1에 의한 ${\varepsilon}$-caprolactam의 분해(分解) 생성물(生成物)은 ${\varepsilon}$-aminocaproic acid로 확인되었다. A bacterium which utilizes ${\varepsilon}$-caprolactam as a sole source of carbon and nitrogen was isolated from sludge of Shinchun river in Taegu and identified as Arthrobacter globiformis N-2-l. The growth medium for the optimum culture condition was composed of 0.4% ${\varepsilon}$-caprolactam, 0.02% $K_2HPO_4$, 0.05% $KH_2PO_4$, 0.02% $MgSO_4{\cdot}7H_2O$, 0.01% $FeCl_3{\cdot}6H_2O$ and 0.05% yeast extract. The optimum pH and temperature for growth were 7.0 and $30^{\circ}C$ respectively. The bacterial growth on the ${\varepsilon}$-caprolactam medium did not require any other organic nitrogen source such as yeast extract, although it was remarkably stimulated by the yeast extract. The bacteria utilized wide range of sugars and organic acids such as ${\alpha}$-ketoglutarate, adipate and P-hydroxybenzoate. The bacteria could use all kind of amino acids, ${\varepsilon}$-Caprolactam in the medium was consumed completely in the timecourse culture at $30^{\circ}C$ for 60 hr on the shaker by the bacteria. Decomposition product of ${\varepsilon}$-caprolactam by Arthrobacter globiformis N-2-1 was ${\varepsilon}$-aminocaproic acid.
대장내시경 전처치로 Sulfate Free-Polyethylene Glycol 복용 후 발생한 허혈성 대장염 1예
유정현 ( Jung Hyun You ),최선택 ( Sun Taek Choi ),김동희 ( Dong Hee Kim ),이동욱 ( Dong Wook Lee ),김유민 ( You Min Kim ),이혜영 ( Hae Young Lee ) 대한장연구학회 2009 Intestinal Research Vol.7 No.2
Ischemic colitis is the most common form of gastrointestinal ischemia. Factors that can contribute to the development of ischemic colitis include atherosclerosis, congestive heart failure, sustained hypovolemia, vasculitis, and mechanical colonic obstruction. Also, pharmacologic agents, including diuretics, pseudoephedrine, non-steroidal anti-inflammatory drugs, and oral contraceptives may be the cause of colonic ischemia. Oral sulfate-free polyethylene glycol (SF-PEG, Colyte-Fⓡ) has frequently been used as a cleaning agent in bowel preparation for colonoscopy. Some cases of ischemic colitis produced by oral hyperosmotic laxatives have been reported; however, no case has been reported regarding ischemic colitis caused by SF-PEG. Herein we report a rare case of ischemic colitis caused by oral sulfate-free polyethylene glycol, which was given for bowel preparation before colonoscopy in a 70-year-old man with chronic constipation. (Intest Res 2009;7:129-132)
은종렬 ( Jong Ryul Eun ),김태년 ( Tae Nyeun Kim ),최선택 ( Sun Taek Choi ),이정훈 ( Jung Hoon Lee ),장병익 ( Byung Ik Jang ),이헌주 ( Heon Ju Lee ),배영경 ( Young Kyung Bae ) 대한내과학회 2007 대한내과학회지 Vol.72 No.5
담관의 막성구조는 국내에서 10예 미만이 보고되어 있을 정도로 매우 드문 질환이며 특징적인 내시경적 역행성 담관조영술 소견으로 진단할 수 있는데 원인은 다양하나 일부는 선천적인 것으로 다른 일부는 염증에 의한 후천성 원인이 제시되고 있다. 특징적인 우상복부 통증으로 내원한 31세 여자 환자에서 내시경적 역행성 담관조영술로 담관결석이 동반된 총간관 막성구조로 인한 협착이 진단되어 Roux-en-Y 간공장문합술로 치료한 예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Biliary web is a very rare disease and it can be diagnosed by its characteristic findings on endoscopic retrograde cholangiogram. The etiology of biliary web is variable. We were able to diagnose a common hepatic duct web in association with choledocholithiasis by performing endoscopic retrograde cholangiogram and operation. A 31-year-old woman visited our emergency room because of colicky right upper abdominal pain she had experienced for several hours. The endoscopic retrograde cholangiogram showed web-like stricture and stone at the common hepatic duct. She was treated by cholecystectomy with Roux-en-Y hepaticojejunostomy. We report here on this rare case along with a review of the literature. (Korean J Med 72:536-539, 2007)