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에탄올에 의해 추출한 황금이 구강암 세포에서 나타나는자가포식작용
최별보라 ( Byul Bo Ra Choi ) 한국치위생학회(구 한국치위생교육학회) 2014 한국치위생학회지 Vol.14 No.2
Objectives : The purpose of the study is to examine the cell growth effect and autophagyeffect of Scutellariae Radix by ethanol extract in SCC 25 cells. Methods : Cell growth inhibitory effect and autophagy induced by Scutellariae Radix wereconfirmed by WST-1 assay, monodansylcadaverine(MDC) stain, and flow cytometry by acridineorange(AO) stain. Results : The Scutellariae Radix treatment decreased the cell proliferation in a dose and timedependent manner. Scutellariae Radix has anticancer effects that autophagic vacuoles were apparentby MDC and AO staining in SCC 25 cells. Conclusions : Scutellariae Radix showed anticancer activity against SCC 25 cells via autophagy. The data provided the possibility that Scutellariae Radix may potentially contribute to oral cancertreatment.
승마추출액을 처리한 G361세포에서의 apoptosis 효과에 대한 연구
최별보라 ( Byul Bo Ra Choi ),김규천 ( Gyoo Cheon Kim ),홍진우 ( Jin Woo Hong ),박상례 ( Sang Rye Park ) 대한예방치과·구강보건학회 2019 大韓口腔保健學會誌 Vol.43 No.2
Objectives: To investigate whether the cytotoxic effect of Cimicifuga rhizoma extract is associated with cell death in the human keratinocyte (HaCaT) and human melanoma cell lines (G361). Methods: Apoptosis induced by Cimicifuga rhizoma extract was confirmed by water-soluble tetrazolium salts-1 (WST-1) assay, immunocytochemistry, and western blot. Additionally, the release of cytochrome c and apoptosis-inducing factor (AIF) was visualized by confocal laser scanning microscopy. Results: The results showed that Cimicifuga rhizoma extract significantly reduced the viability of G361 cells with half-maximal inhibitory concentration (IC 50) of 200 mg/ml, and the apoptotic process was found to occur via the activation of caspase-3 and caspase-9 pathways. Besides, the release of cytochrome c and AIF was also detected. Conclusions: This study suggests that Cimicifuga rhizoma extract causes apoptosis of human melanoma cells through the intrinsic apoptotic pathway.
최별보라 ( Byul Bo Ra Choi ),윤세은 ( Se Eun Yun ),박상례 ( Sang Rye Park ),김규천 ( Gyoo Cheon Kim ) 대한예방치과·구강보건학회 2020 大韓口腔保健學會誌 Vol.44 No.2
Objectives: Dental caries and periodontal disease are infectious and chronic diseases. The aim of the study was to investigate the antimicrobial effect of mentha extracts against Streptococcus mutans (S. mutans ) and Porphyromonas gingivalis (P. gingivalis ). Methods: This activity of mentha extracts were confirmed by the disk diffusion test and minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and colony forming unit (CFU) assays. Results: S. mutans and P. gingivalis showed the highest antimicrobial activity within the inhibition zones. The antimicrobial activity was interrupted as the MIC and MBC of the herbal extracts against the two bacteria were 1 mg/ml and 10 mg/ml, respectively. The antimicrobial effect was determined by the CFU assay. Conclusions: Mentha herb extract demonstrated potential antimicrobial activity against S. mutans and P. gingivalis that cause dental caries and periodontal disease.