Expression of c-fos, c-Ha-ras and Laminin in the Human Stomach and Colorectal Carcinoma Cell Lines

최규실,서영아,김규원,Choi, Kyu-Sil,Suh, Young-Ah,Kim, Kyo-Won 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.6

한국인 위장관 종양에서 확립된 3종류의 위암 세포주(SNU-1, 5, 16)와 7종류의 대장암 세포주 (SNU-61, 67, 70, C1, C2A, C4, C5)를 대상으로 하여 암유전자 및 라미닌 유전자의 발현양상과 종양전이 능력과의 상관관계를 조사하였다. c-fos 암유전자의 경우 일차종양에서 확립된 SNU-C4와 C5에서 발현이 높게 나타나고, c-Haras 암유전자인 경우는 전이 부위에서 확립된 SNU-61 세포주에서 발현이 높으므로 전이성이 낮은 세포주에서 c-fos의 발현이 높고, 전이성이 높은 세포주에서는 c-Ha-ras의 발현이 높다는 종래의 실험결과와 어느 정도 일치함을 나타내었다. 그러나 라미니 유전자인 경우는 SNU-5, C2A, C5 등의 세포주에서 비교적 높게 발현이 되었으나 다른 세포주에서도 고르게 발현이 되고 있으므로 라미닌 유전자의 발현과 종양전이 능력과의 상관성을 SNU 세포주에서는 얻기가 어려운 것으로 추정이 된다. We have investigated a correlation between oncogene and laminin expression and metastatic aggressiveness with three stomach (SNU-1, 5, and 16) and seven colon (SNU-61, 67, 70, C1, C2A, C4, and C5) carcinoma cell lines which were estabilished from primary and metastatic tumor sites. According to Northern blot analysis and immunofluorescence staining, the expression of c fos was elevated in SNU-C4 (primary, colon) and SNU-C5 (primary, colon). The expression of c-Ha-ras was increased in SNU-61 (metastatic, colon). In malignant progression, ras oncogene expression was believed to be responsible for tumor metastasis, whereas the mRNA of c-fos was expressed mainly in the low metastatic clones. In this regard, our results were somewhat in accordance with previous reports. On the other hand, laminin mRNA was detected in all cell lines tested, and among them higher level of laminin mRNA expression was observed in SNU-5 (metastatic, stomach), SNU-C2A (primary, colon) and SNU-C5 (primary, colon). This result suggests that the tumor metastatic behavior can not be simplified to laminin gene expression and the correlation between tumor metastatic aggressiveness and laminin gene expression is more complex.

인간 위장관 종양 세포주에서 c - fos , c - Ha - ras , 그리고 라미닌의 유전자의 발현

최규실,서영아,김규원 ( Kyu Sil Choi,Young Ah Suh,Kyu Won Kim ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.6

We have investigated a correlation between oncogene and laminin expression and metastatic aggressiveness with three stomach (SNU-1, 5, and 16) and seven colon (SNU-61, 67, 70, C1, C2A, C4, and C5) carcinoma cell lines which were estabilished from primary and metastatic tumor sites. According to Northern blot analysis and immunofluorescence staining, the expression of c fos was elevated in SNU-C4 (primary, colon) and SNU-C5 (primary, colon). The expression of c-Ha-ras was increased in SNU-61 (metastatic, colon). In malignant progression, ras oncogene expression was believed to be responsible for tumor metastasis, whereas the mRNA of c-fos was expressed mainly in the low metastatic clones. In this regard, our results were somewhat in accordance with previous reports. On the other hand, laminin mRNA was detected in all cell lines tested, and among them higher level of laminin mRNA expression was observed in SNU-5 (metastatic, stomach), SNU-C2A (primary, colon) and SNU-C5 (primary, colon). This result suggests that the tumor metastatic behavior can not be simplified to laminin gene expression and the correlation between tumor metastatic aggressiveness and laminin gene expression is more complex.

Hypoxia-induced Angiogenesis during Carcinogenesis

최규실,배문경,정주원,문효은,김규원,Choe, Gyu-Sil,Bae, Mun-Gyeong,Jeong, Ju-Won,Mun, Hyo-Eun,Kim, Gyu-Won Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.1

The formation of new blood vessels, angiogenesis, is an essential process during development and disease. Angiogenesis is well known as a crucial step in tumor growth and progression. Angiogenesis is induced by hypoxic conditions and regulated by the hypoxia-inducible factor 1 (HIF-1). The expression of HIF-1 correlates with hypoxia-induced angiogenesis as a result of the induction of the major HIF-1 target gene, vascular endothelial cell growth factor (VEGF). In this review, a brief overview of the mechanism of angiogenesis is discussed, focusing on the regulatory processes of the HIF-1 transcription factor. HIF-1 consists of a constitutively expressed HIF-1 beta(HIF-1β) subunit and an oxygen-regulated HIF-1 alpha(HIF-1α) subunit. The stability and activity of HIF-1α are regulated by the interaction with various proteins, such as pVHL, p53, and p300/CBP as well as by post-translational modifications, hydroxylation, acetylation, and phosphorylation. It was recently reported that HIF-1α binds a co-activator of the AP-1 transciption factor, Jab-1, which inhibits the p53-dependent degradation of HIF-1 and enhances the transcriptional activity of HIF-1 and the subsequent VEGF expression under hypoxic conditions. ARD1 acetylates HIF-1α and stimulates pVHL-mediated ubiquitination of HIF-1α. With a growing knowledge of the molecular mechanisms in this field, novel strategies to prevent tumor angiogenesis can be developed, and form these, new anticancer therapies may arise.

한국어와 독일어의 말(어휘) 형성 비교 연구(1) - 합성어[합성명사]의 형태와 의미 분석 중심 -

최규일 한국어의미학회 2002 한국어 의미학 Vol.10 No.-

반도체 산업의 수율 향상을 위한 Etching Process MMS 개발

최규만,유민식,임대현,안건이 대한산업공학회 2016 대한산업공학회 추계학술대회논문집 Vol.2016 No.11

한국어와 독일어의 말(어휘) 형성론 비교 고찰(2)

최규일 반교어문학회 2002 泮橋語文硏究 Vol.14 No.-

전통주 저장조건에 따른 유기산 변화연구

최규일,김현정,김호진,김효린,김동호,안종성,손영규,송인호 한국식품위생안전성학회 2012 한국식품위생안전성학회지 Vol.27 No.2

This study was conducted to investigate of changes for organic acids in takju during storage conditions. Two types of takju, unsterilized and sterilized, were used. Acetic acid, citric acid, lactic acid, malic acid and succinic acid were detected in sample. Residues of malic acid was not detected after 7 days storage in room temperature(25oC). In contrast to lactic acid was increased by times after storage. Acetic acid concentrations was gradually increased in room temperature but was not changed in cold temperature(4oC). After storage for 10 days, the organic acid residues lactic acid > citric acid > succinic acid > malic acid > acetic acid in unsterilized takju. Compared to unsterilized sample, organic acid residues in sterilized sample was not significantly changed. Consequently, organic acids residues mainly affected by sterilization.

방울토마토중 Dichlofluanid 및 Iprodione의 생산단계별 잔류농약 경시변화

최규일,성기용,정태균,이주환,허장현,고광용,이규승 한국환경농학회 2002 한국환경농학회지 Vol.21 No.4

방울토마토의 생산단계에서의 잔류허용기준 설정과 수확후 저장기간중 경시변화 및 세척에 의한 잔류농약의 감소양상을 파악하였다. 해당약제를 안전사용기준의 기준량 및 배량을 각각 시설재배 포장에서 살포후 10일간 잔류양상을 조사하였고, 실온 및 냉장조건 하에서의 저장실험 및 세척에 따른 전류농약의 잔소량을 파악하여 생산단계부터, 출하, 저장, 소비단계까지의 잔류량을 예측할 수 있는 모델개발을 위한 자료로 이용하고자 연구를 수행하였다. Dichlofluanid와 iprodione은 포장조건에서 반감일수가 기준량 2.2, 3.3일 이었으며, 배량은 3.5, 5.4일로 나타났다. 저장조건하에서의 약제별 반감기는 포장조건보다 잔류량의 감소속도가 느린 것으로 나타나 반감일수가 증가하였고, 세척방법에 의한 잔류농약의 감소량을 조사한 결과 세제 사용시의 평균제거율은 dichlofluanid는 73.7%, iprodione은 64.3%로 나타났고, tap-water 사용시에는 dichlofluanid 73.5%, iprodione 63.5%로 나타났다. 이러한 결과는 작물의 생산단계에서 구한 합리적인 희귀식을 이용하여 잔류농약의 경시변화를 파악하고, 저장 및 세척과정을 거쳐 최종 소비단계에서의 잔류농약의 수준을 평가할 수 있었다. We studied the residual patterns of two fungicides, dichlofluanid and iprodione, in cherry tomato greenhouse after applying with the recommended and double dose. Also, the degradation patterns during storage periods of up to ten days were compared between at room temperature (20℃) and at cold temperature (4℃). Removal rates of fungicides by washing with tap-water and detergent solution (0.1%, 0.2%) were measured. Half-lives of dichlofluanid and iprodione in greenhouse cherrytomato were 2.2~3.5 and 3.3~5.4 days, respectively. During the storage period, the residues were dissipated more slow. Removal rates were 62.8~80.3% by tap-water, 60.4~83.1% by 0.1% detergent solution, and 65.3~77.6% by 0.2% detergent solution. So, we can predict of terminal residues from cultivation period to marketing, storage and consuming.

창의 인성 중심의 과학영재교육을 위한 방향 탐색

최규리 한국과학교육학회 2012 한국과학교육학회지 Vol.32 No.7

The purpose of this study was to investigate the direction of science gifted education based on creativity and character by perception in the gifted education field. Data was collected by in-depth interviews with nine teachers, 10 science gifted students, and their parents, individually or in groups. The results of the study showed that the participants perceived character, creativity, leadership and morality as the important capabilities to have in the future society. Also, they wanted to explore science as much as they wished, form values as a leader, and feel a sense of accomplishment through a collaborative research project. The study suggested that it needed to construct cooperative learning and self-directed scientific investigation for science gifted education based on creativity and character. 본 연구의 목적은 과학 영재들과 학부모, 영재 담당 교사들이 창의 인성 교육에 대해 어떤 인식을 가지고 있는지를 알아보고, 이를 바탕으로 과학 영재들을 위한 창의 인성 교육의 방향을 탐색하는 것이다. 이를 위해 대학부설 영재교육원과 교육청 영재교육원에 재학 중인 학생들과 그 학부모, 영재담당 교사들을 대 상으로 그룹 또는 개별 심층면담을 실시하였다. 연구 결과 연구대상자들은 타인에 대한 배려와 봉사로 함께 어울릴 수 있는 인성, 과학적으로 뛰어난 역량과 사고 능력으로서의 창의성, 추진력과 설득력을 통한 리더십, 그리고 일관성 있는 행동과 실천력으로서의 도덕성을 미래 사회에 필요한 과학영재의 자질이라고 생각하고 있었다. 또한 이들은 영재교육과정 속에서 과학에 대해 마음껏 탐구하고 배우며, 리더로서의 가치관을 형성하고, 친구들과의 협동을 통해 새로운 산출물을 완성해내는 성취감을 얻을 수 있길 바라고 있었다. 이러한 연구결과를 토대로 창의 인성 중심의 과학영재교육은 다양한 과학적 체험과 탐구가 개별적이 아닌 협력적으로 이루어져 완성됨으로써 상호관계 속에서 미래 사회에 요구되는 자질을 키우고 과학영재로서의 성취감을 느낄 수 있게 이루어질 필요가 있음을 제언하였다.

공업계학생 정의교육에 대한 문제성 조사연구

최규서 대한공업교육학회 1983 대한공업교육학회지 Vol.8 No.1