결직장암에서 Met 단백의 발현

최경운,이진숙,이창훈,설미영,서강석 대한병리학회 2000 Journal of Pathology and Translational Medicine Vol.34 No.7

Carcinosarcoma of the Stomach: A Case Report

최경운,이우경,홍성우,장여구,이병모,이혜경 대한위암학회 2013 Journal of gastric cancer Vol.13 No.1

Carcinosarcoma is a rare malignant, biphasic tumor comprised of carcinoma and sarcoma components. In the gastrointestinal tract, carcinosarcoma is most frequently seen in the esophagus and rarely in the stomach. We report a 51-year-old female patient with 2-monthhistory of epigastric pain and dyspepsia. Endoscopic finding revealed a huge ulcerative lesion that infiltrated from the antrum to the mid-body. An endoscopically taken biopsy revealed poorly differentiated malignant round cell neoplasm. After the palliative subtotal gastrectomy, immunohistochemical studies showed two positive reactions for the epithelial marker and mesenchymal marker. Based on the above findings, the patient was diagnosed with gastric carcinosarcoma. The immunohistochemical analysis is a critical method in making an accurate diagnosis of carcinosarcoma.

갑상샘 섬암종의 세침흡인 세포학적 소견 - 온전한 섬모양 구조를 가진 1예 보고 -

최경운,김지연,이진숙,이창훈,Choi, Kyung-Un,Kim, Jee-Yeon,Lee, Jin-Sook,Lee, Chang-Hun 대한세포병리학회 2003 대한세포병리학회지 Vol.14 No.1

Insular carcinoma of the thyroid(ICT) is an uncommon thyroglobulin-producing neoplasm, intermediate between well differentiated and anaplastic carcinoma. Only a few publications have addressed the fine needle aspiration cytologic(FNAC) findings from ICT. We experienced a case histologically diagnosed as ICT and with preoperative FNAC in a 52 - year - old woman. The FNAC displayed scanty colloid and abundant monomorphic follicular cells presented singly, in small loose aggregates, and in cohesive trabecular or acinar clusters. Intact insulae of tumor cells were also identified. Necrosis and mitosis were rare. Tumor cells showed round and monomorphic nuclei, finely granular chromatin, and inconspicuous nucleoli. When insular structure is identified in thyroid FNAC specimen, ICT should be included in the differential diagnosis. Herein we discuss and review the cytologic criteria for separation of ICT from other thyroid neoplasms.

다운사이징을 위한 시스템 개발 방법론에 관한 연구 : 중소기업정보시스템을 중심으로

최경운 慶熙大學校 大學院 1994 高凰論集 Vol.15 No.-

자궁 경부 인유두종 바이러스 감염에 대한 세포검사와 DNA 검사의 비교 연구

최경운,김활웅,김지연,이창훈,설미영 부산대학교 병원 암연구소 2006 부산대병원학술지 Vol.- No.20

Purpose: A newly introduced HPV detection technique in cervical intraepithelial lesion, the HPV DNA Chip test, contains 24 HPV probes and has the advantage of being able to detect 24 HPV types simultaneously. The authors performed HPV genotyping test in cervical specimens from September 2005 to March 2006, and compared their cytologic and histologic diagnoses. Subject and Methods: The HPV DNA Chip test was performed in samples of 263 patients. Among these, 49 patients underwent cervical smear cytology and biopsy. Results: In 160 cases (60.8%) of 263 cases, the HPV DNA was detected. HPV-16 (37.5%), HPV-58 (16.3%), HPV-33 (12.5%), and HPV-18 (10.0%) were frequently detected, including single and multiple infections. The multiple HPV infection was found in 31 cases (11.8%). The sensitivity (91.7%) of the HPV genotyping test using the DNA chip method for detecting HSIL or worse was superior compared with the sensitivity (79.2%) of cytologic diagnosis. Conclusions: Our results indicate that HPV DNA chip test can overcome the low sensitivity of cytologic test and improve the sensitivity of the screening test for cervical intraepithelial lesion.

인공와우수술이 청각장애아동의 언어 발달에 미치는 영향에 대한 사례 연구

최경운 국립특수교육원 2003 특수교육 사례연구 Vol.4 No.-

민간경비종사원의 교육참여가 직무만족 및 서비스 품질에 미치는 영향

최경운 한국산업교육학회 2016 산업교육연구 Vol.32 No.-

본 연구는 민간경비기업에서 실시하는 교육에 대한 종사원의 인식을 분석하고, 이는 직무만족도에 어떠한 영향을 미치며, 궁극적으로 서비스 품질과 어떠한 관계가 있는 가를 파악하는데 그 목적이 있다. 연구를 위해 문헌고찰과 설문조사를 통한 실증분석을 병행하였고, 이에 따른 분석결과는 다음과 같다. 첫째, 가설 1 교육참여가 직무만족도에 미치는 영향을 분석한 결과, 직장내 교육시 참가자의 자율성이 높을수록 전문성 인식 수준이 높아졌다. 교육참여가 직무수행에 미치는 영향에 대한 회귀분석 결과는 직장내 교육시 참가자의 자율성, 지속적 학습문화, 교육 수준이 높을수록 직무수행에 대한 만족도가 높아졌다. 교육참여가 직업전반에 미치는 영향에 대한 회귀분석 결과, 직장내 교육시 지속적 학습문화와 교육 수준이 높을수록 직업 전반에 대한 만족도가 높아졌다. 둘째, 가설 2 교육참여가 서비스 품질에 미치는 영향을 분석한 결과, 교육참여가 공감성 및 응답성에 미치는 영향은 학습 동기와 지속적 학습문화가 높을수록 공감성 및 응답성 수준이 높아졌다. 교육참여가 신뢰성에 미치는 영향을 분석한 결과는 강사 자질 수준이 높을수록 신뢰성이 높아졌다. 교육참여가 확신성에 미치는 영향에 대한 회귀분석 결과, 지속적 학습문화와 강사 자질 수준이 높을수록 확신성이 높아졌다. 셋째, 가설 3 직무 만족도가 서비스 품질에 미치는 영향에 대한 분석 결과를 살펴보면, 직무 만족도가 공감성 및 응답성에 미치는 영향에 대한 회귀분석 결과는 전문성, 직무수행, 직업 전반에 대한 만족도가 높을수록 공감성 및 응답성이 높아졌다. 직무 만족도가 신뢰성에 미치는 영향을 분석한 결과는 전문성, 직무수행, 직업 전반에 대한 만족도가 높을수록 신뢰성이 높아졌다. 직무 만족도가 확신성에 미치는 영향에 대한 회귀분석 결과, 전문성, 직무수행, 직업 전반에 대한 만족도가 높을수록 확신성이 높아졌다. This study aims to analyze employees’ recognition on the education executed in a private security enterprise, and figure out how this affects job satisfaction, and finally what kind of relation it has with service quality. For this study, we executed a positive analysis through literature review and questionnaire survey together, and the result of analysis thereto is as follows. First, as the result of analysis on the effects of education participation on job satisfaction, the higher the participants’ autonomy was in the education within the job office, the higher the level of professionalism recognition got. As the result of regressive analysis on the effects of education participation on job performance, the higher participants’ autonomy, continuous learning culture, and education level were in the education within the job office, the higher the satisfaction as to job performance got. As the result of aggressive analysis on the effects of education participation on overall job, the higher continuous learning culture and education level were in the education within the job office, the higher the satisfaction as to overall job became. Second, as the result of analysis on the effects of education participation of service quality, in the effects of education participation on sympathy and response, the higher learning motive and continuous learning culture were, the higher the level of sympathy and response got. As the result of the effects of education participation on reliability, reliability became higher as the level of an instructor’s quality. As the result of regressive analysis on the effects of education participation on the confidence, the higher a continuous learning culture and the level of an instructor’s quality were, the higher confidence got. Third, in reviewing the result of analysis as to the effects of job satisfaction on service quality, as the result of regressive analysis on the effects of job satisfaction on sympathy and response, the higher satisfaction as to professionalism, job performance, and overall job, the higher sympathy and response became. As the result of analysis on the effects of job satisfaction on reliability, the higher the satisfaction with professionalism, job performance, and overall job was, the higher reliability got. As the result of regressive analysis on the effects of job satisfaction on confidence, the higher the satisfaction as to professionalism, job performance, overall job was, the higher confidence became.

cDNA 마이크로어레이와 면역조직화학염색을 이용한 결장직장암에서의 IGFBP3의 발현 연구

최경운,박도윤,김지연,이진숙,설미영 대한병리학회 2003 Journal of Pathology and Translational Medicine Vol.37 No.3

Background : Insulin-like growth factor binding protein 3 (IGFBP3), a member of six proteins with a high affinity for insulin-like growth factors (IGFs), seems to modulate the effects of IGFs on cells and to regulate cell proliferation through the IGF-independent pathway. We assessed the role of IGFBP3 in the colorectal carcinoma detected by cDNA microarray. Methods : To identify molecular alterations in the colorectal carcinoma, we analyzed gene expression profiles of the colorectal adenocarcinoma by means of a cDNA microarray representing 7,500 genes. Of the differentially expressed genes, the author assessed the insulinlike growth factor binding protein 3 (IGFBP3) gene at the protein level using immunohistochemistry. Results : The expressions of 21 and 16 genes were noted to have more than fivefold increases or decreases in the colonic adenocarcinoma tissue compared with the noncancerous colonic mucosal tissue. The differentially expressed genes include those associated with cell proliferation/apoptosis, signal transduction/transcription, metabolizing enzymes, cytoskeleton, angiogenesis, ion channel, extracellular matrix and others. Of the total 68 cases of colorectal adenocarcinomas observed, 34 cases (50%) showed positive immunohistochemical stainings for IGFBP3. Conclusion : In this study, it is suggested that IGFBP3 plays a role in colorectal carcinogenesis. And combining an immunohistochemistry with a cDNA microarray can facillitate the rapid characterization of a candidate novel molecular target.

연부조직 육종에서 면역조직화학적 예후인자

최경운,김정일,문남훈,Choi, Kyung-Un,Kim, Jeung-Il,Moon, Nam-Hoon 대한근골격종양학회 2008 대한골관절종양학회지 Vol.14 No.2

목적: 세포주기의 조절곤란은 종양의 발생과 진행에 영향을 미친다. 몇가지 알려진 연부조직 육종에 대한 면역 조직화학적 예후인자 들이 있지만 상반된 연구도 있고 G1/S phase관계되는 인자에 대한 연구는 거의 없는 상태이다. 따라서 저자는 연부조직 육종의 재발 및 전이와 관계된 G1/S phase 세포조절주기 단백의 면역화학적 예후인자를 알아보고자 하였다. 대상 및 방법: 1998년 1월부터 2005년 12월까지 연부조직 육종으로 진단된 환자 중 최소한 1년이상 추시관찰이 가능하고 파라핀 블록의 보존상태가 비교적 양호한 43예의 환자를 대상으로 연구하였다. 지방육종 15예, 악성 섬유성조직구증 13예, 횡문근육종 5예, 활막육종 5예, 평활근육종 3예, 섬유육종이 2예였다. 모든 환자의 조직은 전 절제술로 제거된 조직을 대상으로 Cyclin D1, Cyclin E, CDK4, CDK, p16, p27, Rb, E2F-1, p53, Ki-67 등의 면역 조직화학적 발현을 조직 microarray 방법을 사용하여 측정하고 환자의 국소 재발 및 전이에 따른 예후를 비교 분석하였다. 결과: 국소 재발은 8예(19%)에서 일어났으며 이것은 Cyclin E(p=0.024), E2F-1(p=0.046)의 발현과 연관이 있었다. 전이는 16예(37%)에서 일어났으며 CDK4의 증가와 연관이 있었다(p=0.031). 결론: Cyclin E와 E2F-1이 연부조직 육종의 국소 재발과 관련있는 예후를 제공해주었고, CDK4가 전이를 예측할 수 있는 독립적인 예후를 제공해주었다. 따라서 조직 검사시 이들 표식자들의 적절한 사용이 환자의 예후를 예측하고 치료의 범위를 결정 짖는데 중요한 도움을 줄 수 있을 것이다. Purpose: Disturbed cell cycle regulatory proteins are key events underlying the development and/or progression of human malignancies. The aim of this study is to evaluate the protein expression status involved in G1/S cell cycle in human soft tissue sarcoma. Materials and Methods: We simultaneously evaluated the expression of Cyclin D1, Cyclin E, CDK4, CDK2, p16, p27, Rb, E2F1, p53 and Ki-67 by immunohistochemistry in 43 cases of soft tissue sarcoma Results: The Cyclin D1, Cyclin E, CDK4, CDK2, E2F1, and p53 were expressed in 25 (58.1%), 18 (41.9%), 13 (30.2%), 33 (76.7%), 20 (46.5%), and 18 cases (41.9%). The p16, p27, and Rb expressions were decreased in 26 (60.5%), 22 (51.2%) and 19 cases (44.2%). All of the cases showed alterations of more than one out of the above proteins. The increased Cyclin E expression and Ki-67 labeling index (LI) were significantly associated with histologic grade. The Cyclin E and E2F-1 expressions were increased in relapsed cases and the CDK4 expression was increased in cases of metastasis. Conclusion: Alterations of G1/S cell cycle regulatory proteins may play an important role in the tumoriogensis of soft tissue sarcomas. Our results suggest that increased expressions of Cyclin E, E2F1, and CDK4 were associated with tumor relapse or metastasis and could be considered as parameters of prognosis of soft tissue sarcoma.

중소기업을 위한 다운사이징 구축방법론에 관한 연구

최경운,박주석 한국경영정보학회 1994 한국경영정보학회 학술대회논문집 Vol.1994 No.-