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RP-HPLC-PAD를 이용한 갈근(葛根)과 갈근(葛根) 함유 처방의 Puerarin과 Daidzin 분석
초진영 ( Chen Ying Chiao ),권하정 ( Ha Jeong Kwon ),정지선 ( Ji Seon Jeong ),이제현 ( Je Hyun Lee ),홍선표 ( Seon Pyo Hong ) 대한본초학회 2008 大韓本草學會誌 Vol.23 No.4
Objectives: This study presents a reversed-phase high-performance liquid chromatography- pulsed amperometric detection (RP-HPLC-PAD) method for the determination of puerarin and daidzin in Puerariae Radix extract and Chinese medicinal preparations. Methods: Chromatographic separation was performed using a 10% acetonitrile with a reversed-phase column (Unison UK-C18, 100 mm×2.0 mm I.D.; 3 μm). The analyses were detected by pulsed amperometric detector (PAD) in alkaline conditions by combining with post-column NaOH solution. Geniposide was used as an internal standard. Results: The limit of detection (S/N=3) and the limit of quantification (S/N=10) were 0.025 ng, 0.075 ng for puerarin, and 0.05 ng, 0.15 ng for daidzin, respectively. The intra- and inter-day precisions (RSDs) were less than 6.5% and average recoveries of puerarin were 99.7-101.3% and those of daidzin were 101.0-102.8%. Conclusions: According to above results, we developed a determination method for puerarin and daidzin in Puerariae Radix with high sensitivity and selectivitely.