Globulomaxillary Cyst의 1例

朱貞守 대한이비인후과학회 1967 대한이비인후과학회지 두경부외과학 Vol.10 No.3

公共/金融分野 SI事業의 收益性 向上 方案에관한 硏究

주정수,장정주,조헌진 한국경영정보학회 2010 Information systems review Vol.12 No.1

Overexpression of the rice basic leucine zipper transcription factor OsbZIP12 confers drought tolerance to rice and makes seedlings hypersensitive to ABA

주정수,송상익,Youn Hab Lee 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.6

Abiotic stresses such as drought, high salinity,and cold are key factors in determining crop yield. Rice is anotoriously drought-susceptible crop due in part to its smallroot system. Abscisic acid (ABA) involves in abiotic stressresistance in plants. The basic leucine zipper (bZIP) transcriptionfactors play important roles in the ABA signalingpathway in plants. OsbZIP12 is a member of the group EbZIP transcription factor family in rice (Oryza sativa). Expression of OsbZIP12 is induced by abiotic stresses andABA as well as sugar in rice. It is rapidly and stronglyinduced by drought stress. Corresponding with expressionpatterns, several stress and sugar-associated cis-actingelements were found in the OsbZIP12 promoter region. Transgenic rice overexpressing OsbZIP12 showed significantlyimproved drought tolerance and hypersensitivity toABA. The elevated levels of expression of the ABAresponding LEA3 and Rab16 genes in transgenic rice mayenhance drought tolerance. These results indicate thatOsbZIP12 is a positive regulator of ABA signaling anddrought stress tolerance in rice and that OsbZIP12 could beinvolved in crosstalk among stress, hormone, and sugarsignaling. We propose that OsbZIP12 is an importantplayer in rice for conferring ABA-dependent drought toleranceand has high potential for use in the geneticimprovement of stress tolerance.

Abiotic Stress Responsive Rice ASR1 and ASR3 Exhibit Different Tissue-Dependent Sugar and Hormone-Sensitivities

주정수,송상익,이윤합,김연기,남백희 한국분자세포생물학회 2013 Molecules and cells Vol.35 No.5

The expression of the six rice ASR genes is differentially regulated in a tissue-dependent manner according to environmental conditions and reproductive stages. OsASR1 and OsASR3 are the most abundant and are found in most tissues; they are enriched in the leaves and roots, respectively. Coexpression analysis of OsASR1 and OsASR3 and a comparison of the cis-acting elements upstream of OsASR1 and OsASR3 suggested that their expression is regulated in common by abiotic stresses but differently regulated by hormone and sugar signals. The results of quantitative real-time PCR analyses of OsASR1 and OsASR3 expression under various conditions further support this model. The expression of both OsASR1 and OsASR3 was induced by drought stress, which is a major regulator of the expression of all ASR genes in rice. In contrast, ABA is not a common regulator of the expression of these genes. OsASR1 transcription was highly induced by ABA, where-as OsASR3 transcription was strongly induced by GA. In addition, OsASR1 and OsASR3 expression was significantly induced by sucrose and sucrose/glucose treatments, respectively. The induction of gene expression in response to these specific hormone and sugar signals was primarily observed in the major target tissues of these genes (i.e., OsASR1 in leaves and OsASR3 in roots). Our data also showed that the overexpression of either OsASR1 or OsASR3 in transgenic rice plants increased their tolerance to drought and cold stress. Taken together, our results revealed that the transcriptional control of different rice ASR genes exhibit different tissue-dependent sugar and hormone-sensitivities.

평등권에 비추어 본 장애인 고용차별금지

주정수 한국방송통신대학교 2016 인권법연구 Vol.2 No.-

이 논문은 헌법 제11조에 규정되어 있는 기본권인 평등권을 장애인에게도 보장해야 한다는 취지로 고용부문에서 장애인의 차별을 금지하고 장애인에게 일자리를 보장해 주어야 할 것을 연구한 내용이다. 장애인에 대한 정당한 편의제공과 의무고용제도에 관하여 특히 중증장애인의 의무고용 제도의 문제점을 고찰하고 개선방안을 제안하고자 한다. 헌법 제11조는 “모든 국민은 법 앞에 평등하다.”고 규정하였고, 제10조에서는 “모든 국민은 인간으로서의 존엄과 가치를 가지며 행복을 추구할 권리를 가진다.”고 규정하였다. 장애인도 우리나라의 국민이며 인간이기에 비장애인과 똑같이 평등하게 행복한 삶을 누려야 할 권리가 있다. 우리 사회에는 약 2백 50만 명의 장애인이 살고 있다. 장애를 가진 모든 사람들도 비장애인과 똑같이 생명과 인간의 존엄성을 가졌기에 언제나 존중 받아야 할 권리를 가지고 있다. 그러나 장애인을 대하는 우리 사회의 현실은 어떠한가? 지금은 예전보다 장애인에 대한 인식이 많이 좋아지긴 하였지만 아직도 우리 사회에서 장애인을 바라보는 시선은 곱지 않다. 장애인들이 편견 없는 세상에서 좀 더 평등하게 살 수 있도록 하기 위해서는 비장애인들의 많은 포용과 인식의 개선이 절실히 필요하다. 국가의 사회적 책임인 복지 문제와 관련하여 장애인에 대한 은혜적․시혜적 복지도 중요하겠지만 그보다도 헌법 제32조 1항에 “모든 국민은 근로의 권리를 가진다.”고 규정되어 있기 때문에 장애인에게도 ‘근로권’을 부여하고 일자리를 제공하여 스스로 자립할 수 있는 복지가 되어야 한다. 그것이 국가의 사회적 책임의 실현이며, 나아가 ‘사회적 비용’을 줄일 수 있는 적절한 수단이기 때문이다. 본 논문에서는 장애인 고용차별과 관련하여 평등권에 비추어 이러한 문제점과 개선방안을 고찰하고자 한다.

폐광지역에서 분리한 Benzoate 분해세균 Pseudomonas sp. NEQ-1에서 정제된 Catechol 1,2-Dioxygenase의 특성

주정수,윤경하,Joo Jung-Soo,Yoon Kyung-Ha 한국미생물학회 2004 미생물학회지 Vol.40 No.4

Quinoline (2,3-benzopyridine)을 유일한 탄소원과 질소원, 그리고 에너지원으로 이용하는Pseudomonas sp. NEQ-1을 실험 균주로 사용하였으며, 균주로부터 catechol 1,2-dioxygenase (C1,2O)를 유도하기 위하여 탄소원으로 benzoate를사 용하였다. C1,2O의 효소학적 특징을 조사하기 위하여 benzoate에서 배양한 Pseudomonas sp. NFQ-1을 초음파 분쇄기로 파쇄하고, ammonium sulfate침전과 gel permeation chromatography및 Source 15Q의 과정을 실시하여 C1,2O를 분리 및 정제하였다. 정제된 C1,2O의 특이활성(specific activity)은 14.21 unit/mg으로 나타났으며, SDS-PAGE에 의해 조사된 C1,2O의 분자량은 약 33 kDa이었다. Cl,2O는 catechol과 4-methylcatechol 및 3-methylcatechol에 대해서 효소활성을 나타내는 것으로 확인되었다. C1,2O의 Km은 38.54 ${\mu}M$로 측정되었고, Vmax는 $25.10\;{\mu}mol{\cdot}min^{-1}{\cdot}mg^{-1}$으로 나타났다. C1,2O는 $30^{\circ}C$와 pH 8.5에서 최적활성을 나타내는 것으로 조사되었으며, $Ag^+,\;Hg^+,\;Ca^{2+}$,그리고 $Cu^{2+}$는 C1,2O의 활성을 억제하였다. 분석되어진 N-말단 아미노산 서열은 ^1TVKISQSASIQKFFEEA^{17}$이었으며, Pseudomonas aeruginosa PA01과 $82\%$로 가장 높은 유사성을 보였고 Pseudomonas arvilla C-1와는 $71\%,$ Pseudomonas putida KT2440과는 $59\%,$ 그리고 Pseudomonas sp. CA10과는 $53\%$의 상동성이 각각 존재하는 것으로 확인하였다. Our previous research has demonstrated that the bacterium, Pseudomonas sp. NFQ-l capable of utilizing quin­oline (2,3-benzopyridine) as the sole source of carbon, nitrogen, and energy was isolated and characterized [Yoon et ai. (2003) Kor. J. Biotechnol. Bioeng. 18(3):174-179]. In this study, we have found that Pseudomonas sp. NFQ-l could degrade quinoline as well as benzoate, and extended this work to characterize the catechol 1,2­dioxygenase (C1,2O) purified from the bacterium cultured in benzoate media. Initially, C1,2O has been purified by ammonium sulfate precipitation, gel permeation chromatography, and Source 15Q. After Source 15Q, puri­fication fold was increased to approximately 14.21 unit/mg. Molecular weight of C1,2O was about 33 kDa. Physicochemical characteristics (e.g., substrate specificity, Km, Vmax, pH, temperature and effect of inhibitors) of purified C1,2O were examined. C1,2O demonstrated the activity for catechol, 4-methylcatechol and 3-meth­ylcatechol as a substrate, respectively. The Km and Vmax value of C1,2O for catechol was 38.54 ${\mu}M$ and $25.10\;{\mu}mol{\cdot}min^{-1}{\cdot}mg^{-1}.$ The optimal temperature of C1,2O was $30^{\circ}C$ and the optimal pH was approximately 8.5. Metal ions such as $Ag^+,\;Hg^+,\;Ca^{2+},\;and\;Cu^{2+}$ show the inhibitory effect on the activity of C1,2O. N-terminal amino sequence of C1,2O was analyzed as ^1TVKISQSASIQKFFEEA^{17}.$ In this work, we found that the amino acid sequence of NFQ-l showed the sequence homology of 82, 71, 59 and $53\%$ compared with C1,2O from Pseudomonas aeruginosa PA0l, Pseudomonas arvilla C-1., P. putida KT2440 and Pseudomonas sp. CA10, respectively.

제 2 발표장 : 복합재료 얇은 벽보의 강제진동 특성 연구

주정수,송오섭 한국복합재료학회 1995 한국복합재료학회 학술발표대회 논문집 Vol.- No.2

Genetic Organization and Conjugal Plasmid DNA Transfer of pHP69, a Plasmid from a Korean Isolate of Helicobacter pylori

주정수,송재영,백승철,이우곤,조명제,이곤호,윤희상,서지현,이광호,강형련 한국미생물학회 2012 The journal of microbiology Vol.50 No.6

We isolated pHP69, a 9,153 bp plasmid from Helicobacter pylori with a 33.98% (G+C) content. We identified 11 open reading frames (ORFs), including replication initiation protein A (repA), fic (cAMP-induced filamentation protein), mccC, mccB, mobA, mobD, mobB, and mobC, as well as four 22 bp tandem repeat sequences. The nucleic acid and predicted amino acid sequences of these ORFs exhibited significant homology to those of other H. pylori plasmids. pHP69 repA encodes a replication initiation protein and its amino acid sequence is similar to those of replicase proteins from theta-type plasmids. pHP69 contains two types of repeat sequences (R1 and R2), a MOBHEN family mobilization region comprising mobC, mobA, mobB, and mobD, and genes encoding microcin B and C. Among the 36 H. pylori strains containing plasmids, mobA or mccBC are present in 12 or 6, respectively and 3 contain both genes. To examine intrinsic capability of H. pylori for conjugative plasmid transfer, a shuttle vector pBHP69KH containing pHP69 and replication origin of pBR322 was constructed. It was shown that this vector could stably replicate and be mobilized among clinical H. pylori strains and demonstrated to gene transfer by natural plasmid.

Rice ASR1 regulates sugar levels and participates in sugar signaling in roots

주정수,송상익 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.2

The ASR gene is found in various plant species. Although ASR is also expressed in sink tissues, most functional studies on this gene have been performed in source leaves. Here, we report that OsASR1 is involved in the abscisic acid (ABA) response via regulation of sugar levels in the roots. Overexpression of OsASR1 altered root sugar content by increasing fructose levels and decreasing sucrose levels. Moreover, the transcript levels of sugar transporters and sucrose metabolism-related genes were altered in the roots of OsASR1-OX lines. Upon ABA treatment, an additional increase in the glucose and fructose levels was observed in the roots of OsASR1-OX lines compared with ABA-treated NT controls and untreated OsASR1-OX lines. Compared with NT control, the root growth of OsASR1-OX lines was more strongly inhibited by 10 % glucose. Our results suggest that OsASR1 plays an important role in the ABA response and sugar signaling in the roots.

Intergenic transformation of AtMYB44 confers drought stress tolerance in rice seedlings

주정수,오남이,NGUYEN HOAI NGUYEN,이윤합,김연기,송상익,정종주 한국응용생명화학회 2017 Applied Biological Chemistry (Appl Biol Chem) Vol.60 No.4

We transformed rice (Oryza sativa L. Japonica cv. Ilmi) calli with the Arabidopsis transcription factor gene AtMYB44 using Agrobacterium-mediated transformation. The T-DNA construct to be transformed contained tflA cDNA (encoding a toxoflavin lyase) as a selectable marker. Since toxoflavin is a photosensitizing phytotoxin, transgenic plantlets were selected based on their capacity for root development on medium containing this toxin in the light. Homozygous lines were selected by determining the segregation patterns, expression levels, and copy numbers of AtMYB44. Intergenic genomic locations of the inserted T-DNA in the three transgenic lines were confirmed by adaptor-ligation polymerase chain reaction and analysis using FSTVAL (http://bioinfo.mju. ac.kr/fstval/), an open-access web tool used to localize the flanking sequences of the transgene. Drought tolerance of young seedlings of the transgenic lines was determined based on the recovery of wilted leaves by re-watering after 3 days of water deprivation in a 105-well (35W 9 35L 9 45D mm/per well) plate. The three transgenic lines showed average survival rates of 80.4, 93.5, and 72.6%, respectively, whereas wild-type plants failed to recover after re-watering. Thus, the transgenic rice plants exhibited significantly enhanced tolerance to drought stress, as was shown previously in AtMYB44-overexpressing transgenic Arabidopsis and soybean. These results suggest that AtMYB44 activates a drought tolerance mechanism that is conserved in both monocotyledonous and dicotyledonous plants.