http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
단일적응 감자 유전자원들의 역병저항성 및 주요 농업형질 평가
박영은(Young Eun Park),조현묵(Hyun Mook Cho),조지홍(Ji-Hong Cho),조광수(Kwang Soo Cho),김현준(Hyun Jun Kim),후안 란데오(Juan Landeo) 한국원예학회 2011 원예과학기술지 Vol.29 No.5
Phytophthora infestans에 의해 유발되는 감자역병은 전세계의 모든 감자 재배지대에서 발생하는 가장 치명적인 병 중의 하나로서 감자역병에 대한 저항성 품종 육성은 매우 중요한 육종 목표로 인식되고 있다. B3C1집단은 국제감자연구소로부터 도입되었으며 감자역병에 대하여 수평저항성을 가지고 있다. 그러나 이 집단은 페루의 저온단일 환경에서 선발된 계통들로서 고온장일의 국내 환경에 대한 적응성 검정은 이루어진 바 없었다. 따라서 본 연구는 국내의 고온 장일 환경 조건에서 B3C1 계통의 역병저항성과 주요 농업 관련 형질들을 평가하여 국내 재배 가능성을 검토해 보고, 앞으로 역병저항성 품종 육성을 위한 육종 소재로써의 가치를 평가하고자 수행되었다. 무방제 포장에서 역병저항성을 평가한 결과 모든 B3C1 계통들은 매우 높은 역병저항성을 나타내어 역병저항성 자원으로 가치가 높은 것으로 확인되었다. 그러나 모든 B3C1 계통에서 공통적으로 나타나는 극도의 만숙성 괴경 탈립성 불량 괴경 비대 불량 그리고 지상부 및 땅속 줄기의 과번무 현상 등은 반드시 개량되어야 할 것으로 판단되었다. B3C1 계통들은 강릉 지역 보다는 대관령 지역에서 더 높은 적응성을 나타내었으며 최종적으로 20 계통 중 LB-8(CIP393077.159)과 LB-11(CIP393371.159) 2 계통을 선발하였다. 역병에 강하고 국내의 고온장일 환경에 적응성이 높은 계통을 육성하기 위하여 B3C1 계통들은 국내 육성 계통들과 교배를 실시하였으며 지속적인 계통 선발이 진행되고 있다. Potato late blight caused by the fungus Phytophthora infestans is one of the most vital diseases damaging the potato plant. It is for this reason that breeding potato cultivars resistant to late blight is now becoming a major concern around the world. The B3C1 clones has been introduced by the Highland Agriculture Research Center RDA. The clones which came from International Potato Center in 2005 have a durable resistance to late blight. The clones were bred under a short-day condition in Peru. However there was still no report on the adaptability of these clones to the long-day condition in Korea. Therefore this study was conducted to evaluate the late blight resistance and major agronomic characteristics of B3C1 clones under Korea's long-day condition. This study was also done to generate genetic resources for developing new varieties resistant to late blight. In this study it was found out that in naturally infested field with P. infestans AUDPC (area under disease progress curve) values of all B3C1 clones were significantly lower than those of the control varieties,'Superior','Atlantic' and'Haryeong'. It was found out that B3C1 clones had a high level of resistance to late blight and that they could be used as genetic resources to breed potato varieties with late blight resistance. However several undesirable characteristics such as extremely late maturity excessive growth of stems and stolons and production of tubers that cannot easily be removed from the stolons were also observed. Among the twenty B3C1 clones two clones LB-8 (CIP393077.159) and LB-11 (CIP393371.159) were selected for cultivating at the highland area of Korea. Two B3C1 clones were crossed with Korean breeding lines and clonal selection for the progenies is still in progress.
5,7-Dihydroxytryptamine의 세포독성에 의한 고양이 망막내 미세아교세포의 반응양상
주우현,남성안,조승묵,조현후,신민철,원무호,최창도,Joo, Woo-Hyun,Nam, Seong-Ahn,Jo, Seung-Mook,Cho, Hyon-Hoo,Shin, Min-Cheol,Won, Moo-Ho,Choi, Chang-Do 한국현미경학회 1998 Applied microscopy Vol.28 No.4
This study was designed to investigate the microglial reactions to the neurodegenerative changes in the cat retina. All experiments were performed using adult cats of both sex, weighing $2,500g\sim3,500g$. 5,7-DHT $(100{\mu}g)$ dissolved in 0.1% ascorbic acid was injected into the vitreous body. All injections were performed in one-side eye; the other side served as the control, which was injected only with 0.1% ascorbic acid. Cats were sacrificed at 1, 3, 7, 14 and 21 days after intravitreal injection of 5,7-DHT For light microscopy, retinae were fixed with 4% paraformaldehyde and processed using NDPase histochemistry. Same retinae were fixed with 1% para(formaldehyde-2.5% glutaraldehyde and processed for electron microscopy. NDPase-positive microglial cells were mainly distributed in the inner plexiform layer of the retina, and characterized by a small somata with a few slender processes, which were also extended in the ganglion cell layer (GCL) and inner nuclear layer (INL). The intensity of the microglia stained for NDPase was abruptly increased at 7 day as compared with that of the control, and thereafter continuously sustained until 21 day, the last experimental group in this study. Under the electron microscopical observation, microglial cells in the control group exhibited elongate nucleus with perinuclear chromatin condensation, and the perikaryon was scanty. However, a few hypertrophic glial cells were frequently found at 3 days after the drug injection. By 7 day, most microglial cells directed toward the degenerated neurons in the GCL, and the number of microglial cells was slightly increased as compared with the former group. At the 14 day, most microglial cells wrapped the degenerated cells in the GCL, and a few cells showed phagocytotic features. By 21 day, most microglial cells were engaged in phagocytotic activity, and their cytoplasm was filled with the phagorytosed material. Based on the results, 5,7-DHT may act as a specific neurotoxin to the cat retina, and microglial reactions to the neuronal death are already induced in early experimental stage. These results indicate that the microglial cells in the cat retina show characteristic features as a protective effect of neural tissue.