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조직 배양을 통하여 생산된 시호근으로부터 Saikosaponin 의 정량분석
조필형,성락선,배형화,소웅영,조덕이 한국생약학회 1990 생약학회지 Vol.21 No.3
본 연구는 시호의 조직 배양에 의한 대량 증식과 그 대사 산물의 정량분석 및 그 산물의 대량 생산을 위하여 시도되었다. 시호의 잎 배양으로부터 칼루스를 얻고 체세포배를 유도해서 증식시키는데 필요한 2,4-D의 적정농도는 각각 0.1㎎/ℓ및 8㎎/ℓ였다. 부정근의 유도 및 생장에는 호르몬 무처리한 MS배지에 증식시킨 후 현탁 배지에서 배양했을 때 잘 되었다. 체세포배 유래 1년근의 생중략 및 건중량은 증자 유래 1년근 및 2년근 보다 높게 나타났다. HPLC에 의한 saikosaponin의 정량분석 결과, 체세포배 유래 1년근의 경우 saikosaponin a와 c의 함량이 높게 나타났으며 saikosaponin d의 경우는 기내배양 부정근에서 현저히 높게 나타났다.
趙弼衡,成樂宣,裵馨華,蘇雄永,趙德以 全北大學校 基礎科學硏究所 1991 基礎科學 Vol.14 No.2
The study for the quantitative analysis of saikosaponin of Bupleurum falcatum root produced by tissue culture was attempted. The optimal concentration of 2, 4-D was 0.1㎎/l for inducing the callus. The induction and growth of the adventitious root from the callus was obtained in the suspension culture containing MS basal medium supplemented with no plant growth regulators. The results of the quantitative analysis of saikosaponins by high performance liquid chromatography (HPLC) showed that the content of saikosaponin a and c was high in the one-year-old root from somatic embryos and that of saikosaponin d was remarkably high in three-months-old adventitious root from callus.
趙弼衡 圓光大學校大學院 1981 學位論叢 Vol.6-2 No.-
An attempt has been made to evaluate the effect cf antioxidant; butylated hydroxyanisole (BHA), on rat lead poisoning. 360 Female albino rats were divided into 36 experimental groups. All the groups were fed with diets formulated by adding BHA in a range of 0.1% to 3.2%. Aqueous solution of 1% lead acetate was supplied as drinking water to all the experimental groups except the control. Experimental animals from each group were sacrificed on every two weeks interval and twelve various organs such as salivary gland, thymus, lung, heart, liver, pancreas, spleen, kidney, ovary, brain, pituitary and lymph were removed and weighed. Lead sedimentation in kidney tissue was examined through microscopic observation. Intake of food and water was recorded on weekly basis. Serological study including GPT and GOT values were also carried out after sacrificing animals from various groups. It was concluded, after analyzing the data obtained, that the antioxidant BHA is effective for reducing the toxic effect of lead acetate on rats. The reducing effect of BHA on lead poisoning was enhanced significantly by feeding BGA for an enhanced period, namely eight weeks.
랫트의 鉛中毒에 대한 Butyl hydroxyanisole(BHA)의 影響에 관한 硏究
조필형,안영근,김주영 한국환경독성학회 1991 환경독성보건학회지 Vol.6 No.1
The present study was undertaken to examine the effect of butylhydroxyanisole (BHA) on the lead poisoning in Wister female rats. All experimental rats except normal group were fed with diets formulated by adding BHA in a range of 0.1% to 3.2% and aqueous solution of 1% lead acetate ad libitum through the experimental period. The results obtained are summarized as follows: 1) Lead sedimentation in kidney tissue was decreased with increasing experimental period and BHA level of lead plus BHA-treated groups in comparison with that of lead-treated control group. 2) The weights of lung, spleen and left/right (L/R) kidney were significantly de-creased in comparison with those of lead-treated control group after 2 weeks of experimental period, but no difference was shown with those of normal group. 3) The weights of lung, spleen and L/R kidney were increased in lead-treated control group as compared with normal group after 2 weeks of experimental period, but no difference was shown with increasing experimental period. 4) Water intake was remarkably decreased in lead-treated control group as compared with normal group, but water intake by increasing BHA level of lead plus BHA-treated groups showed no significant difference from that of normal group. 5) These results suggest that BHA is effective for reducing the toxic effect of lead in rats.
랫트의 鉛中毒에 대한 Butyl hydroxyanisole(BHA)의 影響에 관한 硏究
조필형,안영근,김주영 원광대학교 식품약품안전성연구소 1991 食品藥品安全性硏究 Vol.4 No.-
The present study was undertaken to examine the effect of butylhydroxyanisole (BHA) on the lead poisoning in Wister female rats. All experimental rats except normal group were fed with diets formulated by adding BHA in a range of 0.1% to 3.2% and aqueous solution of 1% lead acetate ad libitum through the experimental period. The results obtained are summarized as follows: 1) Lead sedimintation in kidney tissue was decreased with increasing experimintal periok and BHA level of lead plus BHA-treated groups in comparison with that of lead-treated control group. 2) The weights of lung, spleen and left/right(L/R) kidney were significantly decreased in comparison with those of lead-treated control group after 2 weeks of experimintal period, but no difference was shown with those of normal group. 3) The weights of lung, spleen and L/R kidney were increased in liad-treated control group as compared with normal troup after 2 weeks of dxperimental period, but no difference was shown with increasing experimintal period. 4) Water intake was remarkably decreased in lead-treated control group as compared with normal group, but water intake by increasing BAH level of lead plus BHA-treated groups showed no significant difference from toat of normal group. 5) These results suggest that BHA is effective for reducing the toxic effect of lead in rats.
조필형(Pil Hyung Jo),성낙선(Rack Seon Seong),배형화(Hyung Hwa Bae),소웅영(Woong Young Soh),조덕이(Duck Yee Cho) 한국생약학회 1990 생약학회지 Vol.21 No.3
The study for the quantitative analysis of saikosaponin of Bupleurum falcatum root produced by tissue culture was attempted. The optimal concentration of 2, 4-D was 0.1 ㎎/ℓ for inducing the callus. The induction and growth of the adventitious root from the callus was obtained in the suspension culture containing MS basal medium supplemented with no plant growth regulators. The results of the quantitative analysis of saikosaponins by high performance liquid chromatography (HPLC) showed that the content of saikosaponin a and c was high in the one-year-old root from somatic embryos and that of saikosaponin d was remarkably high in three-months-old adventitious root from callus.