점 , 선 , 면 / 무제 - 조태훈 -

조태훈 한국기초조형학회 1999 한국기초조형학회 학술발표논문집 Vol.- No.-

수 (數) , 리 (理) , 형 (形) / 무제 - 조태훈 -

조태훈 한국기초조형학회 2000 한국기초조형학회 학술발표논문집 Vol.- No.-

재조함 인성장호르몬의 in vitro 풀림과 재접힘 과정의 구조변화 모니터링

조태훈,채영규,안상점,이은규,Cho, Tae-Hoon,Chai, Young-Kyu,Ahn, Sang-Jeom,Lee, Eun-Kyu 한국생물공학회 1999 KSBB Journal Vol.14 No.6

재조합 인성장호르몬을 사용하여 in vitro 재접힘 공정(풀림, 희석에 의한 공기 중 산화, 그리고 투석)을 수행하였다. 표면소수성이 풀림-재접힘 공정에 중요한 역할을 한다는 것을 형광값의 변화를 통하여 알 수 있었다. 변성제의 intermediate 농도는 Urea와 Gu-HCl 경우 하나의 peak로 SDS와 Sarkosyl의 경우 두개의 peak로 나타났다. 형광값의 변화 중 특이한 점은 Urea의 경우 공기 중 산화와 투석 중의 후반부에 형광값이 증가한다는 것이다. 따라서 공기 중 산화도중 형광값이 증가하기 전에 투석을 시킨 결과 형광값이 증가를 막을 수 있었다. 아직 이 원인에 대해 자세히 알 수 없지만 계속 실험 중에 있다. 이번 실험에서 표면소수성 변화와 연관시켜 fluorescence를 이황화결합에 의한 산화된 형태를 알아보기 위한 방법으로 RP-HPLC를 마지막으로 단백질의 2차원적인 구조를 알아 보기 위해 CD를 사용하였다. CD측정 결과 Gu-HCl보다 SDS의 경우 ${\alpha}$-helices의 파괴가 더 많음을 볼 수 있었다. 재접힘된 rhGH는 본래의 2차원적 구조의 90%이상을 얻을 수 있었다. 이 실험이 기지는 의의는 이 모든 실험결과를 토대로 단백질 재접힘을 모니터링 하였다는 점이다. 즉, 형광값의 변화를 통하여 형광값이 증가하는 것은 표면 소수성이 증가함을 보이는 것으로 단백질의 풀림이 일어난 것이고 3차원적 구조가 깨지고 2차원 구조를 알아 볼 수 있는 ${\alpha}$-helices의 감소를 의미하였다. 이와는 반대로 형광값이 감소하는 것을 통해 재접힘이 일어남을 알 수 있었고, 이러한 결과를 바탕으로 단백질의 재접힘 공정의 변화과정을 형광값을 통하여 모니터링 할 수 있었다. 또한 이 실험의 목적 단백질은 rhGH이지만 다른 단백질에 적용이 될 경우 단백질 재접힘 과정을 수시로 모니터링하고 상태를 예측할 수 있으므로 산업현장에서 소량의 sample로 재접힘 상태를 쉽고 빠르게 판단할 수 있을 것이다. 단백질 재접힘 과정에서 이러한 개념의 성공적 도입은 단백질 회수 수율을 높임으로써 생물분리공정 분야의 기술 발전에 이바지 하리라 사료된다. Using recombinant human growth hormone as a model protein, we carried out unfolding by adding a denaturant such as urea, guanidine HCl, or SDS followed by refolding by dilution and dialysis. The objectives were to monitor the structural changes during in vitro refolding process and, based on the results, to develop a quantitative method of refolding progress assessment. The changes in surface hydrophobicity were measured by fluorescence tagging of 1-anilinonaphthalene-8-sulfonate(1,8-ANS) to the hydrophobic portions, and those in the secondary structure were monitored by using far UV-CD(circular dichroism) spectroscopy. Also, we used RP-HPLC to separate and quantify the folded and unfolded proteins to correlate the result with the structure analysis. Our results indicate the surface hydrophobicity are well correlated with the formations of the secondary structure, primarily ${\alpha}$-helices, as well as the disulfide bridges. We expect this monitoring technique can be applied in industrial fields as a means to quantitatively assess the progress of in-vitro refolding of recombinant proteins.

미래사회에서 예상되는 인간소외의 심화를 극복하기 위한 대학 교양교육개선에 관한 연구

조태훈 한국국민윤리학회 1997 倫理硏究 Vol.36 No.1

국민윤리교육에서의 이념교육의 위상

조태훈 한국국민윤리학회 1988 倫理硏究 Vol.26 No.1

하버마스의 ' 담론윤리학 ' 연구

조태훈 한국국민윤리학회 1999 倫理硏究 Vol.42 No.1

RANSAC을 이용한 다중 평면 피팅의 효율적인 CUDA 구현

조태훈 한국정보통신학회 2019 한국정보통신학회논문지 Vol.23 No.4

As a fiiting method to data with outliers, RANSAC(RANdom SAmple Consensus) based algorithm is widely used in fitting of line, circle, ellipse, etc. CUDA is currently most widely used GPU with massive parallel processing capability. This paper proposes an efficient CUDA implementation of multiple planes fitting using RANSAC with 3d points data, of which one set of 3d points is used for one plane fitting. The performance of the proposed algorithm is demonstrated compared with CPU implementation using both artificially generated data and real 3d heights data of a PCB. The speed-up of the algorithm over CPU seems to be higher in data with lower inlier ratio, more planes to fit, and more points per plane fitting. This method can be easily applied to a wide variety of other fitting applications. 외란(Outlier)이 있는 데이터를 피팅(Fitting)하는 방법으로 RANSAC(RANdom SAmple Consensus)알고리즘이 선, 원, 타원 등 의 피팅에 많이 사용되고 있다. 본 논문은 다수의 평면에 대한 3차원 포인트 데이터가 주어질 때 각 평면에 대해 RANSAC기반 평면 피팅을 최근 딥러닝 등에 많이 사용되는 GPU의 하나인 CUDA를 이용하여 효율적으로 수행하는 알고리즘을 제안한다. 모의 데이터와 실제 데이터를 이용하여 제안된 알고리즘의 성능을 CPU와 비교하여 보인다. 외란이 많고 인라이어(inlier) 비율이 낮을수록 CPU대비 속도가 향상되고 평면의 개수가 많을수록 평면당 데이터개수가 많을수록 병렬처리에 의한 속도가 가속됨을 보인다. 제안된 방법은 다중 평면 피팅외의 다른 피팅에도 쉽게 적용할 수 있다.

노광시스템을 위한 자동 정렬 비젼시스템

조태훈,서재용,Cho, Tai-Hoon,Seo, Jae-Yong 한국반도체디스플레이기술학회 2007 반도체디스플레이기술학회지 Vol.6 No.1

For exposure systems, very accurate alignment between the mask and the substrate is indispensable. In this paper, an automatic alignment system using machine vision for exposure systems is described. Machine vision algorithms are described in detail including extraction of an alignment mark's center position and camera calibration. Methods for extracting parameters for alignment are also presented with some compensation techniques to reduce alignment time. Our alignment system was implemented with a vision system and motion control stages. The performance of the alignment system has been extensively tested with satisfactory results. The performance evaluation shows alignment accuracy of lum within total alignment time of about $2{\sim}3$ seconds including stage moving time.

A machine vision system for inspecting tape-feeder operation

조태훈 한국지능시스템학회 2006 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.6 No.2

A tape feeder of a SMD(Surface Mount Device) mounter is a device that sequentially feeds electronic components on a tape reel to the pick-up system of the mounter. As components are getting much smaller, feeding accuracy of a feeder becomes one of the most important factors for successful component pick-up. Therefore, it is critical to keep the feeding accuracy to a specified level in the assembly and production of eeding accuracy using machine vision. It consists of a feeder base, an image acquisition system, and a personal computer. The image acquisition system is composed of CCD cameras with lens, LED illumination systems, and a frame grabber inside the PC. This system loads up to six feeders at a time and inspects them automatically and sequentially. The inspection software was implemented using Visual C++ on Windows ocess by analyzing the measurement results statistically.

영상예술 교육과정에서 효율적인 미디어 활용과 교육방법

조태훈 경주대학교 1999 論文集 Vol.12 No.2

In the 90s, the concept of education of image art in Korea, an art form based upon visual information, has become more diverse and extended, distinguished from the concept of such general media as television and motion picture. Currently, the history of most art related departments in colleges (including 2-year college) at home that focus upon practical education of dynamic image art is shorter than five years. It appears that the departments short history is because of several factors: 1) it is difficult to distinguish the department from that of drama and motion picture, of journalism and mass media, and that which is related to formative art, 2) it is hard to cover various types of media in a short period time, and 3) it requires purchase of expensive equipment, thus not being practical enough. At present time when the extent of visual information through video and multi-media are important, however, the demand for dynamic image specialists is rapidly increasing. Although social demand for various media is increasing as more and more people have access to computers, those media are not being used rationally at present, which makes it important to resolve the problem rapidly through specific and efficient educational methods. Image art is not simply aesthetic or individual expression; it is a type of media through which an active communication is made with the general public and its techniques continue to be developed with the advancement of popular culture. However, it is more important to focus on videio art, which has been the most efficient and basic art form than to accept all new dynamic art media like a craze. After analyzing existing educational courses of video related classes at the school of fine arts at the university of Wisconsin, Madison, and curriculum of image art related media courses at the art college of Zukuba university in Japan, therefore, I applied them to actual conditions of educational institutions of Korea for image art. Firstly, the courses at the school of fine arts at the university of Wisconsin, Madison and at the Zukuba university are useful for performances and installation art, and for the development of expressiveness and message delivery, respectively. However, those courses are based upon the concept of projects, which is not effective for Korean students who cannot assume high costs and lack basic level of comprehension. For example, installation art generally requires many monitors and video equipment but students often cannot even afford to purchase a video or a movie camera. Also, performance and experimental aesthetics may oly cause confusion for students who do not have specific concept about various image arts, unless they went through such systematic courses as many theoretical studies and basic videos, or had experiences in creative activities. In this respect, it is my conclustion that, for true education of image art, image art needs to be extended from its short history through a gradual process. That is, at first, it is desirable to comprehend basic areas like short films or documentaries through project composition of educational courses or through works that are regenerated in video photographing and monitors in the contents and processes of learning.