제한효소 Bam HI의 필수아미노산 잔기들과 그 역할 때 관한 연구

조진만,권세창,조영동,Cho, Jin-Man,Kwon, Se-Chang,Cho, Young-Dong 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.4

제한효소 BamHI에서 활성에 관여하는 필수적인 아미노산을 알기 위하여 PLP, DEP, DFP와 PG를 효소에 작용시켰다. 효소에 PLP와 DEP을 작용시킨 결과 비활성화 되었으며, 유사 일차반응이었다. 그리고 효소에 PLP와 DEP를 각각 작용시 이차반응속도는 각각 $650M^{-1}min^{-1}$와 $1125M^{-1}min^{-1}$였다. Lysine, histidine, serine과 arginine에 선택적으로 작용하는 PLP, DEP, DFP와 PG를 효소에 각각 반응시 절단능과 결합능의 감소가 일어났다. 효소는 기질에 의하여 상기한 화합물에 의한 비활성화를 억제할 수 있었다. 효소의 비활성화 정도는 각각 PLP와 PG의 농도에 비례하였고, 또한 절단능의 감소와 결합능력의 감소도 각각 정량적인 관계가 있었다. 반면에 DEP와 DFP 농도의 증가에 따른 효소활성의 감소는 절단능과 관계가 없고 결합능의 감소에 기인하였다. 이상과 같은 결과에 의하면 histidine과 serine은 phosphodiester 절단능과 관련이 있고 lysine과 arginine은 결합능에 관여할 것으로 사료된다. To determine amino acid residues essential for the activity of BamHI endonuclease, we have used pyridoxal-5-phosphate(PLP), diethylpyrocarbonate(DEP), diisopropylfluorophosphate (DFP), and phenylglyoxal. Inactivation of BamHI endonuclease by PLP and DEP follows pseudo-first order kinetics. And the second order rate constants for the inactivation of the enzyme were $650M^{-1}min^{-1}$ and $1125M^{-1}min^{-1}$, respectively. Preincubation with group specific reagents for lysine, histidine, serine and arginine residues resulted in the loss of hydrolytic activity and DNA binding function. Furthermore, the presence of inactivation of the enzyme with increasing PLP and phenylglyoxal concentrations correlated very well with quantitative decrease in catalytic activity and binding capacity. However, the complete loss of the enzyme activity with increasing concentrations of DEP and DFP correlated not with decrease in binding capacity, but with decrease in catalytic activity. The cumulative results suggest that histidine and serine residues playa hydrolysis role for the phosphodiester bond, i.e., in the catalytic step, and lysine and arginine residues playa very important role as binding residues in BamHI endonuclease.

제한효소 Bam Hi 의 필수아미노산 잔기들과 그 역할에 관한 연구

조진만,권세창,조영동 ( Jin Man Cho,Se Chang Kwon,Young Dong Cho ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.4

To determine amino acid residues essential for the activity of BamHI endonuclease, we have used pyridoxal-5-phosphate(PLP), diethylpyrocarbonate(DEP), diisopropylfluorophosphate (DFP), and phenylglyoxal. Inactivation of BamHI endonuclease by PLP and DEP follows pseudo-first order kinetics. And the second order rate constants for the inactivation of the enzyme were 650 M^(-1) min^(-1) and 1125 M^(-1) min^(-1), respectively. Preincubation with group specific reagents for lysine, histidine, serine and arginine residues resulted in the loss of hydrolytic activity and DNA binding function. Furthermore, the presence of inactivation of the enzyme with increasing PLP and phenylglyoxal concentrations correlated very well with quantitative decrease in catalytic activity and binding capacity. However, the complete loss of the enzyme activity with increasing concentrations of DEP and DFP correlated not with decrease in binding capacity, but with decrease in catalytic activity. The cumulative results suggest that histidine and serine residues play a hydrolysis role for the phosphodiester bond, i.e., in the catalytic step, and lysine and arginine residues play a very important role as binding residues in BamHI endonuclease.

공동체 문화 활성화를 위한 APT단지 부대복리시설 계획에 관한 연구

이광영,조진만,Lee, Gwang-Young,Cho, Jin-Man 한국주거학회 2008 한국주거학회 논문집 Vol.19 No.2

This study is researched to provide design guidelines of service facilities and programs that apartment residents want along with the increased quality of life. This study is signifiant because it sought effective methods to activate the com-munity culture through service facilities in apartment complexes and the design standard and planning direction for residents. The study results are as follows. In the result of the correlations analysis and multiple regression analysis for the importancy of service facilities in apartment complexes, the following orders were considered as important factor; man-ager office, open space, resident community facility, elderly people meeting and activity facility, walking paths, resident athletic facility, a children's playground, library, and rest areas in the complex. and result of the correlations analysis and multiple regression analysis is the following: service facilities whole importance (y) = 1.413$+0.262^*{\times}1$ (manager office importance)$+0.271^*{\times}2$ (open space importance) $+0.185^*{\times}3$ (resident community facility importance) $+0.136{\times}4$ (elderly people meeting and activity facility importance)$+0.036^*{\times}5$ (walking paths importance) $+0.031^*{\times}6$ (resident athletic facility importance) + $(-0.042)^*{\times}7$ (a children's playground importance) + $(-0.045)^*{\times}8$ (library importance) + $(-0.0097)^*{\times}9$ (rest areas importance).

1인 2표 병립제의 도입과 유권자의 투표행태

조진만(Jin Man Cho),최준영(Jun Young Choi) 한국정치학회 2006 한국정치학회보 Vol.40 No.1

17대 국회의원선거에서 1인 2표 병립제가 새롭게 도입됨에 따라 유권자들은 정당투표와 후보자투표에 대하여 일관투표(straight vote)를 할 것인지 아니면 분할투표(ticket split vote)를 할 것인지 선택할 수 있는 기회를 갖게 되었다. 이러한 선거제도의 변화는 거시적인 차원에서 선거결과에 많은 영향을 미칠 수 있다는 점에서 중요한 정치적 의미를 띠고 있다. 그러나 한국에서 1인 2표제는 도입된 지 얼마 되지 않아 그 정치적 효과에 대한 이론적 논의와 경험적 연구는 아직 미진한 실정이다. 이에 본 연구는 유권자 설문조사 자료를 토대로 유권자들이 1인 2표제에서 어떠한 요인들에 영향을 받아 일관투표와 분할투표 여부를 결정하였는지를 로지스틱 회귀분석을 수행하여 경험적으로 분석하고 있다. 연구결과 1인 2표제에서 유권자의 정치적인 선호를 결정지을 수 있는 준거들-특히 정당과 쟁점(탄핵)-의 강도와 지지하는 정당의 후보자가 지역구선거에서 당선될 가능성이 있는지의 여부가 일관투표와 분할투표 여부를 결정짓는데 중요하게 작용한다는 점을 알 수 있었다. 그리고 군소정당을 지지하는 유권자들의 경우 후보자투표에서 이념적 거리를 고려한 전략적 투표행태를 보이면서 분할투표를 할 가능성이 높다는 점도 확인할 수 있었다. The 17th Congressional Election observed the arrival of the two vote system and the voters were given the opportunity to decide whether or not to split their tickets between party and candidate. This change in the electoral system is expected to have enormous political implications in that it may wield significant influences on the electoral outcomes. Therefore it becomes crucial to pinpoint how the voters behave under the new electoral system. The purpose of this study is to analyze empirically what kind of factors have an impact on the voters' decision of whether or not to split their tickets by employing logistic analysis. The findings indicate that party preferences, impeachment issue, ideology, and electability of the candidate of the preferred party have a significant impact on the decision.

E-ID 카드 현황 및 고려사항

조진만(Jin-Man CHO),진승헌(Jin Seung-Hun),조현숙(Hyun-Suk Jo) 한국정보보호학회 2011 情報保護學會誌 Vol.21 No.4

최근 행정안전부에서 주민등록 관련 법령을 정비하고 주민등록증의 갱신을 준비하고 있다. 이에 전자여권 등 E-ID 카드에 대한 특징, 현황을 살펴보고, E-ID 카드 도입시 검토해야 할 고려사항을 제시하고자 한다.

정치체계의 특성과 의원과 정부의 법안 제안

조진만(Cho Jin Man) 21세기정치학회 2006 21세기 정치학회보 Vol.16 No.3

This study examines whether or not the characteristics of political system have an influence on the performance of legislature, measured by the number of legislative bills. For a comparable purpose, the effects of political system are analyzed with government bills. Thirty one democratic countries are utilized to test the effects of political system. The findings indicate that institutional differences have more significant effect on legislative bill rather than government bills. In addition, a dynamic relationship between institutional factors and the type of bill introduction is also found. This study suggests that in order to improve the performance of legislature it is important to expand the length of session and to abolish standing orders which hinder a legislator's activity.

관상동맥 질환으로 입원한 한국인 환자의 임상양상과 예후인자

조진만 ( Jin-Man Cho ),김종진 ( Chong-Jin Kim ),신우승 ( Woo Seung Shin ),조은주 ( Eun-Ju Cho ),박철수 ( Chul-Soo Park ),김범준 ( Pum Joon Kim ),이종민 ( Jong-Min Lee ),임상현 ( Sang-Hyun Ihm ),임효영 ( Hyou-Young Rhim ),장기육 ( 대한내과학회 2007 대한내과학회지 Vol.73 No.2

Background: Coronary artery disease (CAD) has recently become one of the major causes of mortality and morbidity in Korea. However, not much epidemiologic and demographic data has yet been reported. The purpose of this study was to investigate the clinical features as well as the prognostic factors of patients with CAD. Methods: We prospectively enrolled 1,665 consecutive patients with CAD who had been admitted to the Catholic University Hospitals from December 1999 to April 2003. Results: Acute myocardial infarction (AMI) was the most common cause of admission (n=715, 42.9%). Dyslipidemia, hypertension and smoking were the most common risk factors. More than 70% of the patients who underwent percutaneous coronary intervention (PCI) received stent implantation. A total of 965 (612 males) patients were followed at least for 6 months (the mean follow-up duration was 23.8±12.2 months). The incidence rates of major adverse cardiac events (MACE: cardiac death, acute myocardial infarction, target vessel revascularization) and cardiac death were 15.1% (n=146) and 2.2% (n=21), respectively. There was no difference in overall survival between the patients treated with medical therapy and those treated with PCI. By Cox regression analysis, the independent prognostic factors for MACE were PCI (95% CI: 1.75-4.85; p<0.01) and multivessel disease (95% CI: 1.03-2.04; p<0.05), and the independent prognostic factors for cardiac death were medical therapy (95% CI: 1.08-14.41; p<0.05) and old age (95% CI: 1.13-16.13; p<0.05). Conclusions: There was no difference in overall survival between the patients treated with medical therapy and those treated with PCI. However, PCI was superior to medical therapy for preventing death of the patients with acute coronary syndrome.(Korean J Med 73:142-150, 2007)

의회정치와 신뢰 ; 의회의 집합적 의사결정과 신뢰: 한국 국회의 현실과 선택

조진만 ( Jin Man Cho ) 한국의회발전연구회 2009 의정연구 Vol.27 No.-

왜 한국의 국회는 첨예하게 갈등하고 파행적으로 운영되는 모습을 빈번하게 보여주고 있는가? 이 질문과 관련하여 본 연구는 국회의 집합적 의사결정 구조에 대한 정당들간의 인식 차이가 존재하고, 이것이 국회 내 갈등과 불신을 심화시키는 요인으로 작용하고 있다는 점에 주목하고 있다. 구체적으로 본 연구는 뷰캐넌과 튤락이 제시한 대의민주주의 하의 집합적 의사결정 모델을 활용하여 의회 내 신뢰의 문제는 다수 의사의 존중과 소수 의사의 보호를 최적으로 추구할 수 있는 토대가 된다는 점을 논의하고 있다. 그리고 한국의 국회가 최적의 집합적 의사결정을 이루어내지 못하는 이유는 국회의 집합적 의사결정 구조에 대한 합의가 부족한 상황 속에서 다수 결적 방식과 합의적 방식을 선호하는 정당들이 서로 충돌하기 때문이라는 점을 지적하고 있다. 이를 토대로 본 연구는 한국의 국회가 최적의 집합적 의사결정을 이루어내기 위해서는, 그리고 국회 내 정당들간의 신뢰를 회복하기 위해서는 합의적 의사결정 제도의 도입을 확대하고, 장외투쟁과 물리적 저항 등과 같은 비제도적 수단의 동원을 방지하는 것이 최선의 선택이 될 수 있다는 점을 제안하고 있다. Why the National Assembly of Korea shows the serious disagreements or arguments in its operation? Regarding the question, this study pays attention to the perceptual differences among the parties for the structure of collective decision-making in the National Assembly of Korea. In addition, this study asserts that deepens the conflicts and the distrust in it. To be more specific, this study discusses about the optimal model for collective decision-making in legislative politics based on Buchanan and Tullock`s opinion about it. And then, the trust in legislature forms the basis that makes it possible to respect the will of majority and protect the right of minority. The main reason that can`t make the collective decision-making optimally in the National Assembly of Korea is to fight each other without the consensus about it. In this vein, making the collective decision-making optimally and recovering the trust among the parties are necessary to adopt a more consensual system. It will be helpful to prevent the use of non-institutional means like the outside struggles or physical resistances in the National Assembly of Korea.

한국과 일본의 1인2표 병립제 도입과 효과에 대한 비교연구

조진만 ( Jin Man Cho ) 서강대학교 동아연구소 2008 東亞 硏究 Vol.- No.54

RINDNet을 활용한 SEM Image Edge Detection

조진만(Jin-Man Cho),이혁재(Hyuk-Jae Lee) 대한전자공학회 2023 대한전자공학회 학술대회 Vol.2023 No.6