肺纖維化 過程中 Collagen 및 Fibronectin의 遺傳子 發現과 Collagen 分劃의 變動

조준찬,김인산,조준승 慶北大學校 醫科大學 1990 慶北醫大誌 Vol.31 No.3

Bleomycin-induced lung damage is a well-characterized experimental model for studying pulmonary fibrosis. This model was used to examine α1(Ⅰ), α1(Ⅲ) procollagen and fibronectin mRNA levels and changes of collagen fractions during the development of pulmonary fibrosis. Rats were given 1 unit of bleomycin intratracheally and sacrificed at 4 days, 8 days, and then every week for 4 weeks. The lung tissue was assayed for collagen content by measuring total hydroxyproline content. Characteristics and specific increase of procollagen mRNAs and fibronectin mRNA were determined by northern blot and dot blot hybridization. There was a significant increase of hydroxyproline content of lung tissue following 2 weeks of bleomycin treatment. Northern blot analysis identified a major(5.3kb) and a minor (7.1kb) α1(Ⅰ) procollagen mRNA species and a single fibronectin mRNA species of 8.8kb in size. There was a significant difference among the three mRNAs in the relative increase and the time of maximum accumulation. While the accumulation of procollagen mRNAs was maximum between the 8 days and 14 days, that of fibronectin mRNA peaked in the first week after bleomycin treatment. The procollagen α1(Ⅰ) mRNA accumulated most dramatically(tenfold above the levels in the control lungs) compared with fourfold increases of mRNAs coding α1(Ⅲ) procollagen and fibronectin. In conclusion, specific increases in procollagen mRNAs in lung fibrosis precede increase of tissue collagen content. Type Ⅰ collagen may accumulate much more than type Ⅲ collagen, and the metabolism of tissue fibronectin may be perturbed during pulmonary fibrosis.

人胎盤中 Aminotransferase, Leucine Aminopeptidase 및 Cholinesterase의 含量

曺埈燦,曺準承 慶北大學校 醫科大學 1984 慶北醫大誌 Vol.25 No.2

正常分娩에서 얻어진 人胎盤 35例에 대해서 測定한 AST, ALT, LAP 및 Ch-E의 活性値는 各各 다음과 같았다. AST: 3957±659 Karmen unit/g wet tissue weight(14.8±4.2 Karmen unit/mg protein) ALT: 242±34 Karmen unit/g wet tissue weight(0.75±0.33 Karmen unit/mg protein) AST/ALT=16.7±3.6 LAP: 2112±825 G-R unit/g wet tissue weight(66.5±5.1 G-R unit/mg protein) Ch-E: 13.7 I.U./g wet tissue weight(0.0041±0.0016 I.U./mg protein) Activity of aspartate aminotransferase(AST), alanine aminotransferase(ALT), leucine aminopeptidase(LAP) and cholinesterase(Ch-E) of 36 placenta obtained from normal delivery was determined and following results were obtained. AST: 3957±659 Karmen units/gm wet tissue weight (11.8±4.2 Karmen units/mg protein) ALT: 242±34 Karmen units/gm wet tissue weight (0.75±0.33 Karmen units/mg protein) LAP: 2112±825 G-R units/gm wet tissue weight (66.5±5.1 G-R units/mg protein) Ch-E: 13.7 I.U./gm wet tissue weight (0.0041±0.0061 I.U./mg protein)

식도궤양을 동반한 Behcet 증후군 3例

박승국,안성훈,김약호,허정욱,조준찬 啓明大學校 醫科大學 1985 계명의대학술지 Vol.4 No.2

Behcet's syndrome characterized by recurrent oral and genital ulcerations and occular inflam-mation has been recognized as a multisystemic disease with numerous manifestations including gastrointestinal tract. But the cases associated with esophageal ulceration are reported rarely untill now. Moreover no case was reported in Korea. Here we report 3 cases of Behcet's syndrome with esophageal ulceration with the review of literature. The esophageal ulceration in Behcet's syndrome occurs in the mid to distal esophagus with usually abrupt onset and has a tendency of recurrence. Endoscopic, radiologic and histologic findings of esophageal ulceration in Behcet's syndrome are not distinguishible from regurgitant ulceration, Barrett's ulcer, Crohn's disease and drug-induced ulcer. The cause of esophageal ulceration is to be based on patient's history.

초기폐경기후의 여자에서 혈중 LH, FSH 및 Estradiol-17β의 농도와 그 증상에 관한 연구

조준찬,김원호,김종서,최찬오 啓明大學校 醫科大學 1985 계명의대학술지 Vol.4 No.2

저자들은 1985년 1월부터 1985년 10월까지 계명대학교 동산의료원 내과에 내원한 폐경기후 1년이내 이면서 Kupperman index 증상을 호소하는 36명의 환자에서 증상의 빈도, FSH, LH 및 estradiol-17β의 혈중농도, 증상과 호르몬농도들의 관계를 관찰하여 다음과 같은 결론을 얻었다. 1. 초기폐경후 Kupperman index 증상증 피로감(86%)이 가장 많았고 우울증, 현훈 및 관절통/근육통(각각 75%), 열홍조 및 심계항진(각각 69%), 신경질(67%), 두통(64%), 감각이상(58%), 불면증(44%), 의주감(33%) 등의 순이었다. 2. 초기폐경기후의 혈증 FSH, LH 및 estradiol-17β의 평균농도는 각각 73.4±30.7mIU/㎖, 45.3±24.2mIU/㎖, 51.2±30.2pg/㎖로 FSH와 LH는 가임기여성보다 훨씬 증가했으며 특히 FSH 혈중농도증가가 현저했고 estradiol-17β 농도는 가임기여성보다 50%이상 감소했다. 3. 초기폐경기후 혈중 FSH, LH 및 estradiol-17β농도와 Kupperman index 증상과 관계는 열홍조있는 환자에서 없는 환자에 비해 FSH와 LH혈중농도가 의미있게 (각각 p<0.001, p<0.002) 증가했고 estradiol-17β혈중농도는 의미있게 (p<0.005) 감소했으며 불면증있는 환자에서 없는 환자에 비해 내혈중농도증가(p<0.001)와 estradiol-17β농도감소 (p<0.05)가 통계적으로 의미있고 피로감 있는 환자에서 없는 환자에 비해 estradiol-17β혈중농도가 의미있게 (p<0.05) 감소했으나 다른 증상들과 혈중호르몬 농도들과는 무관하게 나타났다. The frequencies of climacteric symptoms of Kupperman index, serum concentrations of LH, FSH and estradio-17βand the significance of difference in serum levels of LH, FSH and estradiol-17βin postmenopausal women with and without climacteric symptoms were studied in 36 early post-monopausal women(within 1 year after last menstrual bleeding) who visited to internal medical outpatient clinic of Keimyung Unversity Dongsan Medical Center from Jan. 1985 to Oct. 1985. The results were summarized as follows: 1. Among climacteric symptoms of Kupperman index in early postmenopausal women, the commonest is fatigue (86%) and the nexts were depressions. vertigoes and arthralgias myalgias (75% respectively), hot flushes and palpitations (69% respectively), nervousnesses(67%), heada-ches(64%), paresthesias(58%), insomnias(44%) and foamication (33%). 2. The mean serum concentrations of FSH, LH and estradiol-17βin early postmenopausal women were 73.4±30.7 mIU/ml, 45.3±24.2 mIU/ml, 51.2±30.3Pg/ml respectively which were much higher serum concentrations of LH and FSH and 50% lower levels of estradiol-17βthan premeno-pausal women. The increase in serum concentrations of FSH is more prominent than in LH. 3. Serum concentrations of FSH and LH were significantly (p<0.001. p<0.002 respectively) higher and levels of estradiol-17βwere significantly (p<0.005) lower in women complaining of hot flushes than in women without. Serum levels of LH were significantly (p<0/001) higher and levels of estradiol-17βwere significantly (p<0.05) lower than in women without and women with fatigue had significantly (p<0.05) lower estradiol-17βlevels than women without.

원발성 종격동 정상피종 1 예

김윤년,강영우,전영준,송홍석,조진환,조준찬,박이태 대한내과학회 1986 대한내과학회지 Vol.30 No.2

Primary mediastinal seminoma is a rare germ cell tumor histologically identical to testicular seminoma. We experienced one case of Primary mediastinal seminoma recently. He was 51-year old, and complained of progressively severe anterior chest pain and dyspnea for 3 weeks before the time of diagnosis. Echo-cardiogram showed massive pericardial effusion in anterior portion of the right ventricle and posterior portion of the left ventricle with thickened pericardium, During admission, cardiac tamponade was developed, and pericardiectomy was performed. But we couldn`t excised totally because of severe adhesion of anterior mediastinal structures. Pathological findings were pure seminoma developed in thymus with fibrosis and organized hemorrhage in pericardium and pericardial peel. In our case seminoma is associated with possibly tuberculous constrictive effusive pericarditis. We treated with combination chemotherapy followed by irradiation with favorable results.