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Site-directed Immobilization of Antibody onto Solid Surfaces for the Construction of Immunochip
조일훈,최정우,백세환,백의환,이해원 한국생물공학회 2004 Biotechnology and Bioprocess Engineering Vol.9 No.2
The performance of an immuno-analytical system can be assessed in terms of its analytical sensitivity, i.e., the detection limit of an analyte, which is determined by the amount of analyte molecules bound to the capture antibody that has been immobilized onto a solid surface. To increase the number of the binding complexes, we have investigated a site-directed immobilization of an antibody that has the ability to resolve a current problem associated with a random arrangement of the insolubilized immunoglobulin. The binding molecules were chemically reduced to produce thiol groups that were limited at the hinge region, and then, the reduced products were coupled to biotin. This biotinylated antibody was bound to a streptavidin-coated surface via the streptavidin-biotin reaction. This method can control the orientation of the antibody molecules present on a solid surface and also can significantly reduce the possibility of steric hindrance in the antigen-antibody reactions. In a two-site immunoassay, the introduction of the site-directly immobilized antibody as the capture enhanced the sensitivity of analyte detection approximately 10 times compared to that of the antibody randomly coupled to biotin. Such a novel approach would offer a protocol of antibody immobilization in order for the possibility of constructing a high performance immunochip.
유방암 방사선치료에서 Field-in-Field (FIF) 기법의조사면 주변 선량 분석
조일훈,김대홍 한국방사선학회 2022 한국방사선학회 논문지 Vol.16 No.7
The purpose of this study is to confirm the effect of reducing the surface dose around the radiation field in breast cancer radiotherapy using the Field-in-Field (FIF) technique. X-ray was exposed from a linear accelerator (Linac) was used for irradiation, and the surface dose was measured with a glass dosimeter. The source-to-surface distance (SSD) was 90 cm, the field size is 10 × 10 cm2, and the X-ray energy was 6 MV and 10 MV, respectively. The surface dose of the FIF was compared with the dose measured in the physical wedge (PW) and dynamic wedge (DW). Wedge angles of 15° and 30° were used in the PW and DW, respectively. Surface dose was measured at 1 cm, 3 cm, and 5 cm from the center of the field size, respectively. According to the results, FIF showed lower surface dose compared to PW and DW regardless of the energy of the X-ray beam, wedge angle, and dose measurement point. Since FIF could reduce the radiation dose in periphery of the field size in breast cancer treatment, it is expected to be able to reduce the secondary damage caused by the radiation beam as well as to obtain a uniform dose distribution on the target. 본 연구의 목적은 종속조사면 병합 (Field-in-Field, FIF) 방식을 이용하여 유방암 방사선 치료에서 조사면 주변 표면선량 감소 효과를 확인하는 것이다. 방사선 조사를 위해 선형가속기를 사용했으며, 조사면 주변 선량은 유리 선량계로 측정하였다. SSD는 90 cm, 조사면은 10 × 10 cm2, X-선 에너지는 각각 6 MV와 10 MV를 사용했다. FIF 방식의 표면선량과 비교를 위해 금속쐐기(Physical Wedge, PW)와 동적쐐기(Dynamic Wedge, DW) 방식에서 선량을 측정하였다. PW와 DW 방식에서 각각 15°와 30°의 쐐기 각도를 사용하였다. 표면선량은 조사면 중심부에서 각각 1 cm, 3 cm, 5 cm 지점에서 측정하였다. 표면선량 분석 결과 FIF는 PW와 DW와 비교하여 치료 빔의 에너지, 쐐기 각도, 선량 측정 위치에 관계없이 모두 낮은 표면선량 값을 보였다. FIF는 유방암 치료에서 조사면 주변부 선량을 낮출 수 있으므로 표적에 균등한 선량 분포 획득뿐만 아니라 방사선 빔에 의한 2차 장해를 감소시킬 수 있을 것으로 기대된다.
Swelling Ratio Determination of a Polymer Microbead via Video-Microscopy
조일훈,전승근,정현우,박상수 한국고분자학회 2023 폴리머 Vol.47 No.4
A simple video-microscopic method for measuring the swelling ratio of superabsorbent polymer (SAP) microbeads is suggested in this study. A microbead particle was isolated from a women’s sanitary pad and was placed under a video-microscope, followed by the addition of buffered saline onto the microbead. The swelling behavior of the microbead was then videotaped and the images were captured to calculate the projected areas of the microbead for swelling ratio determination. Total of 20 microbeads were studied to measure the swelling ratio at equilibrium, and the ratio was 26.29 (±3.11), which was approximately 10% smaller than the swelling ratio determined by the conventional method, 28.50 (±1.25). Video-microscopic observation of the swelling behavior and estimation of the swelling ratio of SAP microbeads could become an effective tool for understanding the role of SAP microbeads in hygienic and medical products.
무기체계의 고장 이력 데이터를 활용한 소프트웨어 신뢰도 분석 모델 적용 사례 연구
조일훈(Ilhoon Cho),황성국(Seongguk Hwang),이익도(Ikdo Lee),박연경(Yeonkyeong Park),이정훈(Junghoon Lee),신창훈(Changhoon Shin) 한국신뢰성학회 2017 신뢰성응용연구 Vol.17 No.4
Purpose: Recent weapon systems in defense have increased the complexity and importance of software when developing multifunctional equipment. In this study, we analyze the accuracy of the proposed software reliability model when applied to weapon systems. Methods: Determine the similarity between software reliability analysis results (prediction/estimation) utilizing data from developing weapon systems and system failures data during operation of weapon systems. Results: In case of a software reliability prediction model, the predicted failure rate was higher than the actual failure rate, and the estimation model was consistent with actual failure history data. Conclusion: The software prediction model needs to adjust the variables that are appropriate for the domestic weapon system environment. As the reliability of software is increasingly important in the defense industry, continuous efforts are needed to ensure accurate reliability analysis in the development of weapon systems.
능동위상배열 안테나 고장 특성을 고려한 신뢰도 분석 방안 연구
이승상,조일훈,이정훈,박연경 한국신뢰성학회 2018 신뢰성응용연구 Vol.18 No.4
Purpose: There is the limit of the existing methods (k of N model) applied in the reliability analysis of the active phased array antenna. In this study, we propose a new analysis methods considering the failure characteristics. Methods: In this study, the fault characteristics of the active phased array antenna are defined and the cases are summarized. And that simulate failure situations in all cases. Results: As a result of simulations, other cases of failure besides the previously defined failure cases occurred. Conclusion: Therefore, the new method is more accurate than the existing method to perform a reliability analysis method considering fault characteristics of active phased array antenna.