참당귀(當歸) 종자(種字)의 발아율(發芽率) 향상(向上)에 관(關)한 연구(硏究) I. 발아특성(發芽特性)과 발아율(發芽率) 저조(低調) 원인(原因)

조선행,김기준,Cho, Seon-Haeng,Kim, Ki-June 한국약용작물학회 1993 한국약용작물학회지 Vol.1 No.1

참당귀(當歸)(Angelica gigas Nakai) 종자(種子)를 파종(播種)했을 때 발아율(發芽率)이 낮아 종근(種根)의 안정적(安定的) 생산(生産)에 큰 문제점(問題點) 으로 부각(浮刻)되어 있기 때문에 종자(種子)의 발아율(發芽率)을 향상(向上)시킬 방안(方案)을 모색(摸索)하고자 발아특성(發芽特性)과 발아율(發芽率) 저조(低調) 원인(原因)에 대하여 시험(試驗)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 출아율(出芽率은) 추파구(秋播區)에서 66.6%, 춘파구(春播區)에서 41.1%로 (추파구)秋播區에서 높았고 출아시(出芽始)도 춘파구(春播區)보다 (추파구)秋播區가 빨랐다. 2. 저온구(低溫區)$(10^{\circ}C)$나 고온구(高溫區)$(30^{\circ}C)$보다 $20^{\circ}C$구(區)에서 발아율(發芽率) 62.7%, 발아세(發芽勢) 51.7% 발아계수(發芽係數)7.57로 가장 높은 수치(數値)를 보였다. 3. 종자침종시(種子浸種時) 흡수속도(吸水速度)는 수온(水溫)이 높을수록 증가(增加) 하였고, 발아시(發芽時)의 함수양(含水量)은 음건종자(陰乾種子) 무게에 대한 함수종자(含水種子) 무게의 비율(比率)로 각피종자(刻皮種子)는 2.3배(培), 무박피종자(無剝皮種子)는 3.4배(倍)로 나타났다. 4. 종자(種子)의 배유(胚乳)가 클수록 발아율(發芽率)이 증가(增加)하였고 녹색(綠色) 종자(種子)다 갈색(褐色) 종자(種子)가 발아율(發芽率)이 높았다. 5. 저장기간(貯藏期間)에 따른 발아율(發芽率)은 채종(採種) 당년(當年) 종자(種子)가 가장 높고 묵은 종자(種予)일수록 낮아졌으며 실온(室溫)에서 2년간(年間) 저장(貯藏)한 종자(種子)는 전혀 발아(發芽)하지 않았다. 6. 종자내(種子內)에 함유(含有)한 발아(發芽) 및 생장억제물질(生長抑制物質)에 대한 참당귀종자(當歸種子)와 상추종자(種子)로 생물검정(生物檢定)했을때 Methanol 추출물(抽出物)이 가장 효과(效果)가 컸고 다음은 증류수(蒸溜水)이었으며 Ether가 가장 낮았다. This experiment was conducted to study germination characteristics and the decrease cause of germination percent in Angelica gigas Nakai seed. The emergence percent of winter sowing was higher than that of spring sowing as 66.6% and 41.1%, respectively, and the first emergence date was also earlier in winter sowing. The seed germination speed, percent and coefficent showed the highest value at $20^{\circ}C$ of incubation temperature, but lower value at $10^{\circ}C\;and\;30^{\circ}C$. The water uptake speed was increased along with increasing water temperature. The weight of imbibed seed at germination was 3.4times higher based on the weight of intact dry seed and 2.3times on removal of seed coat. In terms of length of seed was large, the germination percent was higher. The germination percent of brown colored seeds showed higher value than that of green colored seeds. The prolonged storage period decreased germination percent. When A.gigas seeds stored at room temperatue for 2years, the seeds were lost their viability. The biological inhibition effect of methanol, water and ether extract on the germination and growth of A.gigas and lettuce seed showed the highest value in the methanol extract, followed by water extract and the least in ether extract.

참당귀(當歸) 종자(種子)의 발아향상(發芽向上)에 관(關)한 연구(硏究) II. 층적(層積), 침종(浸種) 및 Gibberellin 처리(處理)가 발아(發芽)에 미치는 영향(影響)

조선행,김기준,Cho, Seon-Haeng,Kim, Ki-June 한국약용작물학회 1993 한국약용작물학회지 Vol.1 No.2

1989년(年)에 건국대학교(建國大學校) 실습농장(實習農場)에서 재배(栽培)하여 채종(採種)한 참당귀(當歸) 종자(種子)를 재료(材料)로 하여 층적(層積), 침종(浸種) 및 Gibberellin처리(處理)한 후(後) 발아상(發牙床)에 넣어 발아율(發芽率), 발아세(發芽勢) 및 발아계수(發芽係數)를 조사(調査)하여 발아율제고(發芽率提高)를 위한 기초자료(基礎資料)로 활용(活用)하고자 수행(遂行)한 시험결과(試驗結果)를 요약(要約)하면 다음과 같다. 1. 층적처리(層積處理)에 의한 발아율(發芽率)은 무처리(無處理)보다 약(約) 30% 증가(增加)를 보였으며, 저온처리(低溫處理)의 효과(效果)는 인정(認定)되지 않았다. 2. 침종처리(浸種處理)에 의한 발아율(發芽率)은 $20^{\circ}C$, 4일(日) 침종구(浸種區)와 $10^{\circ}C$, 6일(日) 침종구(浸種區)에서 가장 높았으며 환수처리(換水處理)는 무처리(無處理)보다 발아억제물질(發牙抑制物質)의 추출효과(抽出效果)가 컸다. 3. Gibberellin처리(處理)에 의한 발아율(發芽率)은 무처리(無處理)보다 약(約) 20% 증가(增加)를 나타내었으며, 침종구(浸種區)에서 2ppm, 무침종구(無浸種區)에서 5ppm에서 발아율(發芽率)이 가장 높았다. This experiment was conducted to obtain the basic information for the increase ofgermination percent by the treatment of stratification, soaking and $Gibberellin(GA_3)$ in Angelica gigas Seeds caltivated in 1989. The germination percent was increased by 30% with stratification, and the effect of low temperature did not differ significantly. The optimum soaking temperature and periods were found to be 4days at $20^{\circ}C\;and\;6dys\;l0^{\circ}C$ of water temperature. When exchanged water every day during soaking period, inhibitors were more extracted than non-exchange one. The exogenous application of $GA_3$ also increased germination percent about 20% campared with control, and the reasonable level was 2ppm in soaked seed and 5ppm in non-soaked seed.

참당귀 종자의 발아향상에 관한 연구

조선행(Seon Haeng Cho),김기준(Ki June Kim) 한국약용작물학회 1993 한국약용작물학회지 Vol.1 No.2

This experiment was conducted to obtain the basic information for the increase of germination percent by the treatment of stratification, soaking and Gibberellin(GAa) in Angelica gigas Seeds caltivated in 1989. The germination percent was increased by 30% with stratification, and the effect of low temperature did not differ significantly. The optimum soaking temperature and periods were found to be 4days at 20℃ and 6days 10℃ of water temperature. When exchanged water every day during soaking period, inhibitors were more extracted than non-exchange one. The exogenous application of GA₃ also increased germination percent about 20% campared with control, and the reasonable level was 2ppm in soaked seed and 5ppm in non-soaked seed.

참당귀의 생육단계별 GA₃ , IAA 및 함량변화

조선행(Seon Haeng Cho),신국현(Kuk Hyon Shin),안상득(Sang Deuk Ahn) 한국약용작물학회 1994 한국약용작물학회지 Vol.2 No.1

This study was carried out to observe the changes in gibberellin(GA₃), indol-3 acetatic acid(IAA) and abscisic acid(ABA) contents known as plant growth regulators in the leaves of Angelica gigas and to get the basic information for the establishment of cultural practice for the inhibition of floral induction. Changes in GA₃, IAA and ABA contents were investgated at different growth stage in the young flag leaves of two year old plants. The contents of GA₃ showed the highest value by 4.87㎎/㎏ at early flowering stage, and those were gradually increased from vegetative stage to early flowering stage, but greatly decreased from early flowering stage to fruiting stage. The contents of IAA were also the highest by 2.27㎎/㎏ at early flowering stage. The contents of ABA were gradually increased along with the development of growth stage. In the fruiting stage those were the highest value by 0.37㎎/㎏ and in early flowering stage the contents were decreased temporarily.

Lignan contents in Acanthopanax senticosus by HPLC

김혜민,조선행,국순자,이상현,Kim, Hye-Min,Cho, Seon-Haeng,Kook, Soon-Ja,Lee, Sang-Hyun Institute of Agricultural Science 2011 Korean Journal of Agricultural Science Vol.38 No.3

A reverse-phase system of HPLC using a linear gradient of acetonitrile and deionized water was developed for the quantification lignans, eleutherosides B and E, in Acanthopanax senticosus. The HPLC system consisted of linear gradient of acetonitrile and deionized water, and UV/VIS detection was set at 210 nm. Both eleutherosides B and E contents in different parts of A. senticosus were determined. As a result, the contents of eleutherosides B and E were measured in the leaves (trace amounts and 0.029 mg/g, respectively), stems (0.107 and 1.015 mg/g, respectively), roots (0.026 and 0.390 mg/g, respectively), and fruits (0.022 and 0.043 mg/g, respectively). Moreover, eleutherosides B and E in the water extract were found 0.011 and 0.171 mg/g, respectively.

오가피 설기의 재료 배합비에 따른 관능적 텍스처 특성

최영심(Young-Sim Choi),조선행(Seon Haeng Cho),지옥화(Ok Kwa Jhee) 동아시아식생활학회 2008 동아시아식생활학회지 Vol.18 No.6

Lignan contents in Acanthopanax senticosus by HPLC

Hye Min Kim(김혜민),Seon Haeng Cho(조선행),Soon Ja Kook(국순자),Sanghyun Lee(이상현) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.3

A reverse-phase system of HPLC using a linear gradient of acetonitrile and deionized water was developed for the quantification lignans, eleutherosides B and E, in Acanthopanax senticosus. The HPLC system consisted of linear gradient of acetonitrile and deionized water, and UV/VIS detection was set at 210 nm. Both eleutherosides B and E contents in different parts of A. senticosus were determined. As a result, the contents of eleutherosides B and E were measured in the leaves (trace amounts and 0.029 mg/g, respectively), stems (0.107 and 1.015 mg/g, respectively), roots (0.026 and 0.390 mg/g, respectively), and fruits (0.022 and 0.043 mg/g, respectively). Moreover, eleutherosides B and E in the water extract were found 0.011 and 0.171 mg/g, respectively.

실과 교실원예 프로그램이 학업 성취에 미치는 효과

김장수,조선행,김형균 韓國實科敎育硏究學會 2002 實科敎育硏究 Vol.8 No.1

The purpose of this study was to examine educational effectiveness for the classroom horticulture program that can be utilized in the culture domain of practical arts in elementary school. The subjects in this study were students in two sixth grade class of elementary school. The statistical technique used for the academic achievement were percentage and t-test. The findings of this study were as follows: (1) when the two class were compared with each other after experiment, the experiment class were significantly better in cognitive achievement than the control class; (2) the culture skill achievement of the experiment class students was higher than that of control class students; (3) most students of the experiment class responded that the classroom horticulture program was very enjoyable and attractive. A summarized above, It was found that the classroom horticulture program for the academic achievement by the elementary school students in practical arts was more effective than the conventional one.