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Aujeszky`s Disease Virus의 실험적 감염 Rat의 병리조직학적 관찰과 면역조직화학적 항원 검출
조우영 ( Woo Young Cho ),조부제 ( Bu Je Cho ),조성환 ( Sung Whan Cho ),이철호 ( Chul Ho Lee ) 한국가축위생학회 1990 韓國家畜衛生學會誌 Vol.13 No.1
In order to investigate the clinico-pathological and immunohistochemical changes in the rats infected with Aujeszky`s disease virus(ADV), 100 heads of 4 weeks-old rats were inoculated intraperitoneally and intranasally, with the domestically isolated ADV, NYJ-1-87 strain, at 10(3.0) or 105.0TCID50/0.2m1. Results obtained through the experiments were summarized as follows: 1. Clinical signs such as dulness, anorexia, pruritus, fascial edema, dyspnea and ataxia were observed from the 2nd day and died at the 3rd to 5th day after ADV inoculation. By necropsy, congestion and hemorrhage were observed in the abdominal organs, while no specific changes were detected in the other organs. 2. In histopathological observation, degeneration and necrosis of the nervous cells, non-suppurative meningoencephalitis, microgliosis and perivascular cuffing were manifested in central nerve system but no specific changes were observed in the other organs. 3. By immunohistochemical staining using peroxidase antiperoxidase, the positive cells were detected in the tissues of kideny, spleen, urinary bladder and lung.
Aujeszky's disease virus 국내분리주 접종자돈의 병리발생에 관한 연구 I. 병리학적 및 전자현미경적 관찰
조우영,조성환,김재훈,박최규,황의경,조부제,정운선 한국동물위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.1
This study was conducted to elucidate the pathogenesis of Aujeszky's disease virus(ADV) by histopathologic examination. The first Korean ADV Isolate, which was isolated from piglets with clinical signs of Aujeszky's disease in Yangsan(YS) county, Kyungnam province, was inoculated into 32 days old piglets with a dose of $10^{5.9}$$TCID_{50}/ml$ through intranasal or intramuscular route. These piglets were sacrificed at intervals of every 24hrs for 8 days. The virulence of YS strain was determined by the observation of clinical signs, gross findings, and histopathologic changes in tissues. The virus recovery test was performed from brain, spleen, lung and tonsil in cell culture. The pathogenesis of YS strain was determined by the observation of histopathologlc lesions in CNS and neuronal tracts. The major clinical signs were fever, anorexia, dyspnea, constipation, tremor, ataxia, circling movement, hindleg paralysis and salivation. The clinical signs were more severe in piglets of the group inoculated intranasally than those of the intramuscularly inoculated gorup. Lymphocytopenia was detected on day 5 to day 6 postinoculation (PI). The ADV was recovered from the tissue homogenates of tonsil, lung, spleen and cerebrum in cell culture. The highest virus titer was detected from tonsil between day 6 and day 7 PI. Reddish sublobar consolidation foci were scattered in the apical and cardiac lobes of lung. Although yellowish necrotic foci were detected in tonsil and liver, hemorrhagic lesions were mainly observed in heart, kidney and lymph nodes. Histopathologically, degeneration and necrosis of nerve cells, nonsuppurative meningoe-ncephalitis, nodular gliosis and perivascular cuffings were observed in CNS. Multifocal fibronecrotic foci were observed in lung, liver, lymph nodes and spleen. The major pathologic changes were detected in the midbrain, pons and medulla oblongata. Eosinophilic intranuclear inclusion bodies were mainly observed in epithelia and /or macrophages of tonsil, liver, lung, spleen and submandibular lymph nodes, and neurons of brain, respectively. Observation of viral particles at various stages of replication were possible from the endothelial cells of the alveolar capillaries and tonsillar crypt epithelia by transmission electron microscope.
RT-PCR 기법을 이용한 분변내 소 코로나바이러스 검출
안재문 ( Jae Moon Ahn ),조우영 ( Woo Young Cho ),이종인 ( Jong In Lee ),조부제 ( Bu Je Cho ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.3
The reverse transcriptase polymerase chain reaction (RT-PCR) was used for the detection of bovine coronavirus (BCV) in fecal samples by using reverse transcriptase and two primers which flanked M gene sequence of 407bp. RT-PCR detected bovine coronavirus specifically, but did not detect mouse hepatitis virus (MHV), transmissible gastroenteritis virus (TGEV), and bovine rotavirus (BRV). The M gene sequences of MHV are homologus to that of BCV, but minor differences exist in the primer regions, preventing annealing of the primers. Detection of BCV using RT-PCR was compared with ELISA and the agreement of BCV detection by RT-PCR and ELISA was 95.3%. RNA detection in positive clinical specimens was significantly better by PCR than immunological detection of BCV by ELISA.
박재명 ( Jae Myoung Park ),최해연 ( Hae Yean Choi ),이은정 ( Eun Jeong Lee ),조우영 ( Woo Young Cho ),조부제 ( Boo Je Cho ),정운선 ( Un Sun Chung ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.2
In an effort to improve the quality of meat and to monitor farms, residual tetracyclines in local beef and pork produced in the province of North Chung-cheong were determined by a EEC 4-plate, Charm Ⅱ and HPLC, respectively. The results were summarized as follows; 1. Of the 547 samples, 4 beef and 13 pork samples were judged to be positive by EEC 4 -plate method. 2. Detection rates of tetracyclines by type 1(+ + - -) and type 2(+ + + or + + ± -) micro-bial growth inhibition in EEC 4-plate method were 100% and 71%, respectively. 3. Of 17 positive samples, 6 were positive for tetracyclines, 4 were positive for tetracyclines and sulfonamides, 1 was positive for sulfonamides, and 2 were positive for others by Charm Ⅱ test. 4. The best eluents were 0.01M methanolic oxalic acid, and the ideal temperature for stable concentration was 40℃ as optimal HPLC analytical conditions for the detection of tetracy-clines. 5. Of the 10 positive samples for tetracyclines by Charm Ⅱ test, tetracyclines were confirmed in 2 beef and 6 pork samples, using HPLC, at levels ranging from 3.64~4.22 ppm and 0.2~1.20 ppm, respectively.
최해연 ( Hae Yean Choi ),박재명 ( Jae Myong Park ),이은정 ( Un Jeong Lee ),조우영 ( Woo Young Cho ),이종인 ( Jong In Lee ),조부제 ( Bu Jae Cho ),정운선 ( Un Sun Chung ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.2
To study the efficacy monoclonal antibody(MAb) against bovine rotavirus(BCV) in treatment of calf diarrhea, the MAb was fed to 166 calves with diarrhea from Chung-buk area. The results were summarized as follows. 1. Among the 1,049 calves investigated, 166(16%) calves were infected with BCV. 2. The monthly rate of BCV infection were higher in October to December compared with other months of the year. 3. Among the 166 calves with diarrhea, 137(83%) calves were recovered. 4. Young calves within 7-day-old were more effective in treatment than other ages and the rate of treatment was 86%. 5. Most effective period for treatment of rotavirus was at the first stage of infection.
Aujeszky`s disease virus 국내분리주 접종자돈의 병리발생에 관한 연구: 1. 병리조직학적 및 전자현미경적 관찰
조우영 ( Woo Young Cho ),조성환 ( Sung Whan Cho ),김재훈 ( Jae Hoon Kim ),박최규 ( Choi Gui Park ),황의경 ( Eui Kyung Hwang ),조부제 ( Boo Jue Cho ),정운선 ( Un Sun Chung ) 한국가축위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.1
This study was conducted to elucidate the pathogenesis of Aujeszky`s disease virus(ADV) by histopathologic examination. The first Korean ADV isolate, which was isolated from piglets with clinical signs of Aujeszky`s disease in Yangsan(YS) county, Kyungnam province, was inoculated into 32 days old piglets with a dose of 10(5.9)TCID50/mℓ through intranasal or intramuscular route. These piglets were sacrificed at intervals of every 24hrs for 8 days. The virulence of YS strain was determined by the observation of clinical signs, gross findings, and histopathologic changes in tissues. The virus recovery test was performed from brain, spleen, lung and tonsil in cell culture. The pathogenesis of YS strain was determined by the observation of histopathologic lesions in CNS and neurorial tracts. The major clinical signs were fever, anorexia, dyspnea, constipation, tremor, ataxia, circling movement, hindleg paralysis and salivation. The clinical signs were more severe in piglets of the group inoculated intranasally than those of the intramuscularly inoculated gorup. Lymphocytopenia was detected on day 5 to day 6 postinoculation (PI). The ADV was recovered from the tissue homogenates of tonsil, lung, spleen and cerebrum in cell culture. The highest virus titer was detected from tonsil between day 6 and day 7 PI. Reddish sublobar consolidation foci were scattered in the apical and cardiac lobes of lung. Although yellowish necrotic foci were detected in tonsil and liver, hemorrhagic lesions were mainly observed in heart, kidney and lymph nodes. Histopathologically, degeneration and necrosis of nerve cells, nonsuppurative meningoencephalitis, nodular gliosis and perivascular cuffings were observed in CNS. Multifocal fibronecrotic foci were observed in lung, liver, lymph nodes and spleen. The major pathologic changes were detected in the midbrain, pons and medulla oblongata. Eosinophilic intranuclear inclusion bodies were mainly observed in epithelia and/or macrophages of tonsil, liver, lung, spleen and submandibular lymph nodes, and neurons of brain, respectively. Observation of viral particles at various stages of replication were possible from the endothelial cells of the alveolar capillaries and tonsillar crypt epithelia by transmission electron microscope.