우리나라 당뇨환자들의 민간요법 실태

조미란(Cho Mi Ran),조여원(Choue Ryowon) 한국영양학회 1998 Journal of Nutrition and Health Vol.31 No.7

산도에 따른 봉독의 성분 및 생리활성에 대한 안정성

조미란 ( Mi Ran Cho ),한상미 ( Sang Mi Han ),김정민 ( Jung Min Kim ),여주홍 ( Joo Hong Yeo ),홍인표 ( In Phyo Hong ),우순옥 ( Soon Ok Woo ),이광길 ( Kwang Gill Lee ) 한국잠사학회 2014 한국잠사곤충학회지 Vol.52 No.1

서양종 꿀벌로부터 봉독채집장치를 이용하여 채집한 정제봉독의 pH 안정성에 대하여 평가하고자 멜리틴의 함량, 단백질의 변화, 항균력, 세포증식률을 측정하였다. 정제봉독을 pH2에서 pH9까지 24시간 동안 처리한 후 멜리틴함량과 단백질의 변화, S. aureus, P. acnes, S. mutans와 E. coli에 대한 항균력, 피부세포인 HDF에 대한 세포증식률에 미치는 영향을 분석하였다. pH2에서부터 pH9까지 각각 처리한 정제봉독의 멜리틴 및 단백질 성분의 변화는 확인되지 않았다. 봉독의 주요 약리효과인 항균력에 있어서도 시험균주마다 다소 차이가 있었으나 S. aureus, P. acnes, S. mutans와 E. coli 모두 산도에 따른 유의할 만한 변화는 확인되지 않았다. 또한 피부세포 증식률에 있어서도 산도에 따른 변화는 확인되지 않았다. 이러한 결과로 미루어 봉독의 pH처리는 멜리틴 함량, 단백질의 변화, 항균력 및 세포증식률에 영향을 주지 않는 것으로 확인되어 봉독은 산도의 변화에 있어 안정성이 매우 높은 것으로 사료된다. This study was for the investigation of the stability of purified bee venom (PBV) during the treatment in the pH range from pH2 to pH9 for 24 hours, respectively. Changes of components and physiological functionalities in PBV were by evaluated silver staining, and melittin contents were measured by liquid chromatography. The antimicrobial activity against bacteria by minimum inhibitory concentration (MIC) and effect of the cell regeneration were measured by 3- [4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay using human dermal fibroblast (HDF) cell. The main proteins such as melittin and phospholipase A2 showed no characteristic changes. The antimicrobial activity and effect of cell regeneration showed no difference from pH2 to pH9. From this study, we suggest that components and physiological functionalities of PBV against treated pH were kept stability at from pH2 to pH9.

고려홍삼의 콜라겐 유도 관절염의 예방과 억제효과

조미란(Mi-Ran Cho),왕옥철(Yutie Wang),장진선(Jin Sun Jang),김채균(Chaekyun Kim) 고려인삼학회 2009 Journal of Ginseng Research Vol.33 No.2

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation and progressive cartilage and bone erosion. Korean red ginseng (KRG) has been shown to have an anti-inflammatory effect by inhibiting the secretion of inflammatory cytokines like TNF-α, IL-1, -6, and -8, and IFN-γ. In this study, whether KRG extract has an inhibitory effect on the collagen-inducible development of arthritis in DBA/1J mice was investigated. To induce arthritis, type Ⅱ collagen emulsified in Complete Freund’s Adjuvant was intradermally injected into the base of the tails of mice. Three weeks after the initial injection, a booster injection of type Ⅱ collagen emulsified in Incomplete Freund’s Adjuvant was administered. The oral administration of KRG extract for 8~10 weeks at the dose of 300 mg/kg (three days a week) inhibited the development of arthritis in the experimental group, compared to the control group which was given saline. While the administration of KRG extract three times a week demonstrated both preventive and inhibitory effects, the administration of KRG extract once a week had little inhibitory effect. In other studies, the regimen of KRG administration has been shown to decrease the plasma level of inflammatory cytokines like IL-8 and TNF-α, but the plasma levels of these cytokines were not decreased in the present study. The results of the present study suggest that KRG has preventive and inhibitory effects on collagen-induced arthritis.

국내 리모델링의 문제점 및 개선방향

조미란(Cho, Mi Ran) 한국콘크리트학회 2003 콘크리트학회지 Vol.15 No.4

경장영양액의 섬유소가 환자의 배변양상에 미치는 영향

조미란 ( Mi Ran Cho ),이정숙 ( Jung Sook Lee ),김명천 ( Myung Cheon Kim ) 한국정맥경장영양학회 2012 한국정맥경장영양학회지 Vol.5 No.1

Purpose: Diarrhea is generally recognized as one of the most common complications associated with tube feeding. As a result, various forms of fibers are added to the formula to reduce the incidence of tube-feeding-related diarrhea. The purpose of this study was to compare the frequency of liquid stool in enterally fed patients receiving an insoluble fiber-enriched formula and those fed a mixed fibers diet formula. Methods: We conducted a prospective, double-blind trial to determine the effects of administration of enteral nutrition containing insoluble fiber on the stool frequency. Eight patients were given soluble and insoluble group (SIF group), and 11 patients were given the insoluble fiber formula (IF group) for consecutively 7 days. A number of stool and fecal score were recorded for all 7 days by using the King`s stool chart with an absolute daily fecal score of 15. Results: Calorie was started on the first day with 625± 170.6 (150∼1,400) kcal/day for SIF group and 827.3± 140.8 (300∼1,200) kcal/day for IF group. On the seventh day, these values were increased to 74.9±6.6 (44.4∼ 100)% for SIF group and 87.0±4.6 (66.7∼107.1)% for IF group of nutritional goal. SIF group reported a significantly higher tendency towards the mean number of stools per day (2.8±0.7 [0.7∼6.7]), mean fecal score per day (16.3±3.8 [4.6∼36.0]) and mean number of days with diarrhea per week (3.1±1.0 [0∼7]) than IF groups with corresponding values of (1.8±0.2 [0.6∼2.9], 10.3± 2.0 [2.6∼19.7] and 1.9±0.5 [0∼4]). Conclusion: The results of this study suggest that enteral nutrition with insoluble fiber is beneficial in reducing the incidence of diarrhea in patients. Because gastrointestinal functional status (e.g., delayed gastric emptying) and intestinal environment (e.g., antibiotics) of patients with enteral tube feeding may be different from that of healthy humans, gastrointestinal symptoms (e.g., stool aspects) may vary depending on the fiber in enteral formula. (JKSPEN 2013;5(1):31-35).

인슐린 비의존형(Type Ⅱ) 당뇨환자에서 누에분말 섭취가 혈당 및 혈중지질농도에 미치는 영향

조미란(Cho Mi Ran),조여원(Choue Ryowon),정성현(Chung Sung Hun),류재환(Ryu Jae Whan) 韓國營養學會 1998 Journal of Nutrition and Health Vol.31 No.7

GC를 이용한 축산물 가공품 중 보존료 동시분석법

이미순 ( Mi Soon Lee ),조미란 ( Mi Ran Cho ),김영욱 ( Young Wook Kim ),성인경 ( In Kyung Sung ),노진형 ( Jin Hyeong Noh ),이정선 ( Jung Sun Yi ),윤소영 ( So Young Yune ),문희정 ( Hee Jung Moon ),박재우 ( Jae Woo Park ),최효정 ( Hy 한국수의공중보건학회 2008 예방수의학회지 Vol.32 No.3

APo E3 Genotype 고지혈증 환자에서 사상체질에 따른 의학영양치료 혈중 지질 농도에 미치는 영향

문보경,조미란,이혜옥,송일병,조여원,Moon, Bo-Kyoung,Cho, Mi-Ran,Lee, Hei-Ok,Song, Il-Byung,Choue, Ryo-Won 사상체질의학회 2003 사상체질의학회지 Vol.15 No.1

본 연구는 고지혈증 환자를 대상으로 apo E 유전자 다형성에 따른 사상체질의 분포양상을 살펴보고 apo E3 타입 고지혈증 환자에서 사상체질에 따른 의학영양 치료가 혈중 지질 농도에 미치는 영향을 살펴보았다. (1) 본 연구에 참여한 고지혈증 환자 33명으로 남자의 평균 연령은 $47.8{\pm}6.2$세였고, 여자의 평균 연령은 $50.5{\pm}5.7$세였다. apo E 유전자 다형성의 분포는 apo E2는 6.1%(2명), apo E3는 78.8%(26명), apo E4는 15.2%(5명)였고, 사상체질의 분포는 태양인이 0%(0명), 태음인이 60.6%(20명), 소양인이 21.2%(7명), 소음인이 18.2%(6명)였다. (2) 신체 계측 결과는 apo E3 타입 고지혈증 환자 중 남자의 경우는 신장, 체중, 체지방, 제지방, TBW, BMI, 허리둘레, 엉덩이둘레, 허리둘레/엉덩이둘레 비율 모두에서 MNT 실시 전과 후의 변화가 나타나지 않았다. 여자의 경우는 소양인에서 체중, BMI가 MNT 전과 비교하여 후에 유의적인 감소가 있었고, 태음인과 소음인에서는 MNT 실시 전과 후의 변화가 나타나지 않았다. (3) 영양소 섭취 상태는 남자의 경우 태음인에서 총열량, 지방, 동물성 지방, 식물성 지방, 섬유소, 비타민 $B_1$, 비타민 $B_2$, niacin의 섭취가 유의적으로 감소하였고 소양인에서는 섭취량의 변화가 없었으며, 소음인에서는 인의 섭취가 유의적으로 증가하였다. 여자의 경우 태음인에서 칼슘, 인의 섭취가 유의적으로 증가하였고, 소양인과 소음인에서는 섭취량의 변화가 없었다. MNT 실시 전과 후의 영양소 섭취 상태는 총열량의 섭취량이 처방열량에 적절하게 개선되었고, MNT 후에 태음인에서는 칼슘, 철분, 비타민 A의 섭취가 개선되었으며, 소음인에서는 단백질, 비타민 A의 섭취가 개선되었다. 식사 전반의 질은 태음인, 소양인, 소음인 모두에서 잘 유지되었으나 식품 섭취 패턴을 조사한 결과는 콜레스테롤이 많은 음식, 소고기, 돼지고기 등 육류, 단음식, 튀긴 음식의 섭취 빈도에서 변화가 관찰되지 않았다. (4) 혈중 지질 농도의 변화는 태음인에서 HDL-콜레스테롤의 농도는 유의적으로 증가하였고, 호모시스테인의 농도는 유의적으로 감소하였다. 소양이네서 HDL-콜레스테롤의 농도가 유의적으로 증가하였다. 소음인에서는 혈중 지질 농도에서 유의적인 변화가 없었다. The purpose of this study was to investigate the effects of medical nutrition therapy(MNT) on plasma lipid levels of hyperlipidemic patients with apo E3 genotype according to Sasang Constitutions. From March to July, 2001, the 33 hyperlipidemic patients admitted to K University Medical Center were studied. The study subjects were classified according to their Sasang constitutions by QSCC II questionnaire which have been used at K University Oriental Medical Center. The anthropometric assessments, blood analysis, and apo E genetic typing were carried out. Nutrient intake was determined by food record method of food taken during two weekdays and one weekend. The MNT including the instruction for hypolipidemic and hypocholesterolemic diet (step l diet) was performed for 12 weeks. The results are as follow; (1) The mean age of hyperlipidemic patients was $49.91{\pm}8.48$ years. (2) The distributions of Sasang Constitution were 60.6% of Tae-eum, 21.2% of So-yang, and 18.2% of So-eum. The distributions of apo E genotype were 6.5% of apo E2/3, 78.8% of apo E 3/3, and 15.2% of apo E 3/4. (3) The nutrient consumption of the apo E3 subjects before the MNT showed lower calorie, iron, calcium, and vitamin B2 intakes than the RDAs for each nutrients with no significant differences among the constitutions. After 12-week of MNT, only the fat consumption was decreased in the Tae-eum group. The MNT did not change the pattern of food intake. (4) The plasma level of triglyceride, total cholesterol, and LDL-C were not changed after MNT in the three constitutional groups. The level of HDL-C was significantly increased significantly in Tae-eum and So-yang group and the level of homocystein was lowered in Tae-eum group after MNT. It could be concluded that the 12-weeks MNT with hypolipidemic and hypocholesterolemic diet did not change the level of total cholesterol, triglyceride, and LDL-C effectively regardless of Sasang constitutions even though the subjects' dietary intake was improved by MNT.

동유럽 국가산 수입 유가공품의 방사능 잔류조사

이명헌,조미란,김연희,손성완,김상근,Lee, Myoung-heon,Cho, Mi-ran,Kim, Yeon-hee,Son, Seong-wan,Kim, Sang-keun 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.3

The present studies were conducted to monitor radioactive contamination in dairy products imported from 16 countries located in the East Europe which were affected by the Chernobyl nuclear accident. The 556 samples such as butter, cheese, ice cream, whey protein and hydrolysed milk protein products were collected randomly and determined from 1999 to 2002. All sample were below the Koeran and CODEX maximum tolerance level of radioactivity for $^{131}I$, $^{134}Cs$ and $^{137}Cs$.

65세 이상 노인에서 사상체질에 따른 식습관, 영양소 섭취상태 및 질병 양상에 관한 연구(II)

김연경,조미란,장린,조여원,Kim, Youn-Kyoung,Cho, Mi-Ran,Chang, Rin,Choue, Ryo-Won 대한지역사회영양학회 2002 대한지역사회영양학회지 Vol.7 No.1

Since the 1970s, the average life expectancy of Koreans has been increasing. Our concerns are not simply about longevity, but also about disability-free life. lately, Sasang constitutional medicine has attracted public attention, especially with respect to the elderly. The purpose of this study was to investigate the food habits, nutrients intake, and disease distribution among the elderly, according to their Sasang constitutions. The results are as follow : The distribution of constitutions of the subjects were Tae-eum 48.8%, So-yang 27.4%, So-eum 22.6%, and So-yang + So-eum 1.2%. In both elderly men and women, body weight, percent ideal body weight, and diastolic pressure of the Tae-eum were significantly higher than those of other constitutions. In both men and women, the level of hemoglobin in the Tae-eum was significantly higher than those of other constitutions. The level of LDL-cholesterol was higher, and the level of HDL-cholesterol was lower in the So-eum elderly women. Food habits of the So-eum were the most irregular, and nutrients intake of the So-eum was lower than that of other constitutions in both elderly men and women. In elderly men, the incidence of hypertension was the highest in the Tae-eum, and the incidence of gastritis was the highest in both So-yang and So-eum. In elderly women, the incidence of fatty liver was the highest in the Tae-eum and So-yang, and gastritis and hypertension were the highest in the So-eum. In the elderly, obese and anemic persons were significantly more in the Tae-eum and the So-eum, respectively. The results of this study showed that there were significant differences of physical, characeristics, food, habits, and distributions of disease for the subjects according to each Sasang constitutions. Intake of nutrient was poor, regardless of Sasang constitutions, in both men and women.