BALB/c 마우스에서 DNCB-유도 아토피 피부염 유사병변에 대한 Curcumin 투여와 630 nm LED 광치료의 병용 효과

제갈승주 ( Seung-joo Jekal ),박미숙 ( Mi-suk Park ),김대중 ( Dae-jung Kim ) 대한임상검사과학회 2017 대한임상검사과학회지(KJCLS) Vol.49 No.2

아토피피부염은 만성 염증성 피부질환으로 습진성병변, 피부건조 및 가려움증의 특징적 증상을 보이는 질환이다. 현재 사용되고 있는 아토피피부염 치료약제들은 오래 사용시 여러 부작용이 있어서 이를 대신할 새로운 대체 치료법의 개발이 요구되고 있다. Curcumin은 항염, 항알러지와 같은 효과를 나타내는 천연 폴리페놀(polyphenol)이며, 발광다이오드(light emitting diode, LED) 광치료는 아토피피부염과 상처치유에 효과가 있는 것으로 알려져 있다. 본 연구는 DNCB로 유도한BALB/c 마우스의 아토피피부염에서 curcumin 투여와 630 nm LED 조사를 병용하여 그 치료 효과를 높일 수 있는가를 조사하기 위해 시도하였다. 아토피피부염의 치료 효과를 조사하기 위해 modified SCORAD 지수, 조직병리학적 분석, 면역조직화학염색 및 TUNEL 검사를 사용하였다. 그 결과 curcumin 투여와 630 nm LED 조사가 각각 SCORAD 지수, 비만세포 및 탈과립세포의수, 호산구 수, 표피세포의 증식능 및 자멸세포의 수를 의미 있게 감소시키고, 비만세포의 탈과립을 억제하여 섬유모세포에 의한 콜라겐 생성을 의미 있게 감소시켰으며, curcumin 투여와 LED 조사를 병용하였을 경우 단일 처리군에 비해 이들 수치가 의미 있게 감소하는 것을 확인하였다. 이러한 결과는 아토피피부염 치료에 curcumin 투여와 LED 조사가 각각 효과가 있음을 의미하며, 병용 치료 시 그 효과가 좀 더 향상된다는 것을 의미하는 것으로, 아토피피부염의 대체치료 전략으로 curcumin 투여와 630 nm 조사의 병용 치료를 제안한다. Atopic dermatitis (AD) is a chronic inflammatory skin disease. It is characterized by eczematous lesions, skin dryness, and pruritus. The existing treatment drugs for AD have side effects, especially if the drugs are taken for extended periods. Therefore, new alternative therapies are necessary. The aim of this study was to investigate the combined effects of curcumin admi-nistration and LED irradiation on AD. AD-like lesions were induced in BALB/c mice by repeated application of 1-chloro-2,4-dinitrobenzene (DNCB) to the shaved skin of the ear and neck. Thirty male BALB/c mice were divided into five groups: vehicle, DNCB, curcumin, LED, and curcumin+LED groups. Curcumin (0.1 g/kg/day) was administrated repeatedly during a period of 14 days (experimental period) and 630 nm LED irradiation (5 J/cm²/day) was performed in the acryl box once a day for 10 days, after inducing AD-like lesions via DNCB application. The severity of AD-like lesions was evaluated during the experimental period, using a modified SCORAD index. Both ear and neck skin tissues were examined histologically for epidermal thickness, mast cell, eosinophil counting, and dermal collagen density. Epidermal cell proliferation and apoptosis were detected using immunohistochemistry and TUNEL, respectively. These were all reduced in SCORAD index, epidermal thickness, collagen density, number of mast cell and eosinophil in dermis, and number of proliferating cell and apoptotic cell in epidermis by curcumin administration and 630 nm LED irradiation. Moreover, all parameters were significantly lower in the curcumin+LED group compared with the curcumin group and LED group. These results suggest that the combined therapy of curcumin and LED is more effective than a single treatment. We recommend that this can be a feasible alternative therapy to manage AD.

자궁경부의 정상조직과 상피냐종양 및 침윤암종에서핵소체형부위

제갈승주 ( Seung Joo Jekal ),정주연 ( Ju Youn Chang ),곽효일 ( Hyo Il Kwag ),노장섭 ( Jung Sup Ro ) 대한임상검사과학회 1995 대한임상검사과학회지(KJCLS) Vol.27 No.1

위점막에서 헤마톡살련 염색시간을 연장한 메이어헤마톡살린-에오신염색에 의한 Helicobacter pylori-likeorganism 검출률과 특수 염색법들과의 비교

제갈승주 ( Seung Joo Jekal ),차현희 ( Hyun Hee Cha ),김신무 ( Shin Moo Kim ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2

Helicobacter pylori-like organism(HLO) is more easily visua1ized with specia1 stainings than with hematoxylin-eosin, but this is time-consumed, expensive to use the routine diagnostic workup. We performed to verify whether Mayer``s hematoxylin-eosin staining method with prolonged hematoxylin time compares favorably with other well established specia1 stainings in the detection of HLO in gastic biopsy. Fifty gastric biopsy specimens(48 from antrum, two from body) , routinely forma1in f1xed and paraff1n wax embedded, from gastritis patients were stained with modified Harris`` hematoxylin-eosin(m-Harris HE) and modified Mayer``s hematoxylin-eosin m-Mayer HE) with prolonged hematoxyrlin time(10min), Warthin-Starry, Genta, AgNOR and EI-Zimaity staining. The detection rate for HLO was examined by divide into three groups (mild, moderate, severe) according to the degree of inflammation in the biopsy specimens. Density of HLO was scored from 0 to 5 by Genta``s c1assification. Using Warthin-Starry as a standard, positivity for HLO was 52% (26/50). Relative sensitivity for Warthin-Starry staining was 100% with m-Mayer HE, 96% with Genta, 96% with EI-Zimaity, 92% with AgNOR, and 80% with m-Harris HE staining. Relative sensitivity of HLO density for Warthin-stany stainings was lower at low (grade 1) density(Genta, 96%; EI-Zimaity. 96%; AgNOR, 33%, routine Harris HE, 0%) than at high(grade 2 to 5) density except case with m-Mayer HE(100%). Detection rate and density of HLO also appeared a significantly high according to increase the degree of inflammation. m-Mayer HE staining has a sensitiviη comparable to thoes of Warthin-Starry and Genta staining and is simple, inexpensive compared with the other staining methods in detecting HLO. Therefore, it is vety useful and practica1 for identifying HW in routine gastric biopsy specimens.

일반 파라핀 절편에서 wet autoclave 전처리 후 AgNORs의 검출

제갈승주 ( Seung Joo Jekal ),곽효일 ( Hyo Il Kwag ),노종섭 ( Jong Sup Ro ),이형섭 ( Hyung Sup Lee ) 대한임상검사과학회 1997 대한임상검사과학회지(KJCLS) Vol.29 No.1

Helicobacter pylori 감염 위염에서의 비만세포 증가와 Stem Cell Factor Receptor (c-kit)의 발현

제갈승주 ( Seung Joo Jekal ) 대한임상검사과학회 2005 대한임상검사과학회지(KJCLS) Vol.37 No.1

It is known that mast cells (MCs) are increased in H. pylori-infected gastritis and its increase is mediated by stem cell factor (c-kit ligand). To determine the mechanism of mast cell recruitment and activation by stem cell factor, weinvestigated the expression of stem cell factor receptor (c-kit) in H. pylori-positive and -negative gastric mucosa. Biopsy specimens from 16 H. pylori-negative and 20 positive subjects were examined. H. pylori infection in gastric mucosa was examined by the Warthin-Starry method. MC and c-kit were identified by immunohistochemisty, using a monoclonal antihuman MC tryptase antibody and a polyclonal anti-human c-kit antibody. Densities of MC and c-kit positive cell were measured by a computerized image analysis system. MCs were detected in the lamina propria of both H. pylori-positive and -negative gastric mucosa. Densities of MC and c-kit positive cell were significantly greater in H. pylori-positive than -negative subjects. c-kit was located on the surface of MCs. These results indicate that stem cell factors may be one of the factors involved in mast cell increase and that they activate mast cells by binding with c-kit.

C57BL/6 마우스에서 고지방 식이와 CCl4로 유발한 간섬유증에 미치는 커큐민의 보호효과

제갈승주 ( Seung Joo Jekal ),민병운 ( Byung Woon Min ),박호 ( Ho Park ) 대한임상검사과학회 2015 대한임상검사과학회지(KJCLS) Vol.47 No.4

Curcumin, a major polyphenolic compound of turmeric, is well known to prevent non-alcoholic steatohepatitis (NASH) related to obesity. The aim of the study was to investigate the effect of curcumin on hepatic fibrosis induced by carbon tetrachloride (CCl4) in obese mice. CCl4 was administrated in mice fed a normal diet (ND) or a high fat diet (HFD) for 7 weeks together with or without curcumin. It was conducted to examine for metabolic profiles, adipocyte size, and liver fibrosis by serum biochemistry, histology and immunohistochemistry. Also, Apoptosis of hepatic cells was determined by the TUNEL method. Treatment with curcumin significantly lowered the body weight, fasting glucose, serum AST and ALT, and decreased the adipocyte size, the number of macrophage and mast cells in adipose tissue, and collagen deposition in liver tissue in the HFD+CCl4 group compared with the findings of the HFD+CCl4 group. In contrast, treatment with curcumin on the ND+CCl4 group did not show a significant difference except the body weight and mast cell number when compared with the ND+CCl4 group. Furthermore, curcumin significantly reduced the number of parenchymal apoptotic cells, whereas it increased the number of non-parenchymal apoptotic cells, especially resembling an activated hepatic stellate cell in the liver. Taken together, this data suggests that curcumin might be an effective antifibrotic drug for the prevention of liver disease progression in obese mice. Thus, the development of curcumin as a therapy for obesity and liver fibrosis is supported.

자궁경부상피내종양과 침윤성 편평상피암종의 혈관신생에서 비만세포와 혈관내피성장인자의 발현

제갈승주 ( Seung Joo Jekal ),이정아 ( Jung Ah Lee ),노종섭 ( Jong Sup Rho ) 대한임상검사과학회 2005 대한임상검사과학회지(KJCLS) Vol.37 No.3

To determine the correlation between mast cells(MCs) and neoangiogenesis in the growth and progression of cervical cancer, we investigated mast cell density(MCD), microvessel density(MVD) and the expression of vascular epithelial growth factor(VEGF) in cervical intraepithelial neoplasia and invasive suqamous cell carcinoma of the uterine cervix. Forty-five cervical intraepithelial neoplasia(CIN I, II and III), 15 microinvasive carcinomas, 15 invasive squamous cell carcinomas and 20 normal cervical epithelia were included in this study. MCs were stained with anti-c-Kit antibody and alcian blue, microvessels with anti-factor VIII antibody and VEGF with anti-VEGF antibody. The adjacent fields of both normal and neoplastic epithelium were used for counting MCs and microvessels. Computerized image analysis was used to evaluate MCD and MVD. MCD and MVD were the mean numbers per 1mm2 counted in 5-10 high and low power fields respectively. In both c-Kit and alcian blue stained sections, MCD progressively increased along the continuum from CIN I to invasive squamous cell carcinoma(p<0.001). MVD increased significantly with cervical neoplasia progression, from CIN to invasive squamous cell carcinoma (p<0.001). In double c-Kit and Factor VIII-stained sections, MCs were mainly present in the areas adjacent to newly formed blood vessels. However, there were no significant differences in MCD and MVD between normal epithelum and CIN I. A strong correlation was also observed between MCD and MVD. In double VEGF and alcian blue-stained sections, VEGF was expressed in only MCs. Strong VEGF-positive MCs were particularly abundant around the tumorous region. Our results suggest that MCs may upregulate neoangiogenesis by VGEF secretion in the development and progression of cervical neoplasia

스트렙토조토신 유도 당뇨 흰쥐에서 전기자극이 상처치유와 피부 비만세포에 미치는 영향

제갈승주 ( Seung Joo Jekal ),이경선 ( Kyung Sun Lee ),정옥봉 ( Ok Bong Chung ),이재형 ( Jae Hyoung Lee ) 대한임상검사과학회 2008 대한임상검사과학회지(KJCLS) Vol.40 No.2

The aim of this study was to investigate the effect of electrical stimulation on healing of impaired wound and alteration of mast cells in experimental diabetic rats. Thirty male Sprague-Dawley rats were divided into three groups : incision (control), diabetes+incision (diabetes) and diabetes + incision + electrical stimulation (D/ES). Diabetes was induced in rats by streptozotocin (STZ) injection (60 mg/kg, one time) and 20 mm length incision wounds were created on the back after shaving hair. The electrical stimulation rats were treated with a current intensity of 30~50 V at 120 pps and 140 μs for 10 days from 3 days after STZ injection. The lesion and adjacent skin tissues were fixed with 10% buffered formalin, embedded with paraffin. For wound healing analysis, hematoxylin-eosin (HE) and picrosirius red staining were performed. Mast cells (MC) were stained with toluidine blue (pH 0.5) and quantified at ×200 using a light microscope. The density of keratinocyte proliferation and microvessels in skin tissues were analyzed using a computerized image analysis system on sections immunostained with proliferative cell nuclear antigen (PCNA) and α-smooth muscle actin (α-SMA), respectively. The results showed that the wound healing rate, collagen density and neoepidermis thickness, density of PCNA-positive cells and density of α-SMA-positive vessels were significantly higher in D/ES rats than in diabetic rats. The density of MCs and degranulated MCs in D/ES rats were also significantly higher than those in diabetic rats. These findings suggest that the electrical stimulation may promote the tissue repair process by accelerating collagen production, keratinocyte proliferation and angiogenesis in the diabetic rats, and MCs are required for wound healing of skin in rats.

앓은 막 크로마토그래피에 의한 제조회사별 Pyronin Y분석의 의의: 조직 염색성과의 관련성

제갈승주 ( Seung Joo Jekal ),오현숙 ( Hyun Sook Oh ),최영자 ( Young Ja Choi ),조혁종 ( Hyeok Jong Jo ),박충서 ( Choong Soe Park ) 대한임상검사과학회 1994 대한임상검사과학회지(KJCLS) Vol.26 No.1

자궁경부의 편평상피내병소와 편평상피세포암종에서의 비만세포에 관한 연구

제갈승주 ( Seung Joo Jekal ),차현희 ( Hyun Hee Cha ),최영자 ( Young Ja Choi ),노종섭 ( Jong Sup Roh ),곽효일 ( Hyo Il Kwak ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2

Mast cells have recently been found to be well correlated with the inhibition of invasiveness and metastasis in various cancer. This work aimed to investigate the number of mast cells and ratio of mast cell granulation in squamous intraepithelial lesions and squamous cell carcinoma of the cervix. Cervical biopsy specimens were divided into low grade squamous intraepithelial lesion(n=20), high grade squamous intraepithelial lesion(n=75) and squamous cell carcinoma(n=40) by The Bethesda System. The sections were cut from routinely formalin- f1xed and paraffin-embedded tissues and stained with 0.5% toluidine blue(pHO.5)-nuc1ear fast red. The mast cells were quantif1cated the numbers per square millimeter using a computerized image analysis system. Mean number of mast cells was signif1cantly higher in high grade squamous intraepithelial lesion than in low grade squamous intraepithelial lesion and signif1cantly lower in squamous cell carcinoma than in high grade squamous intraepithelial lesion(p<0.01). The percent of mast cell ganulation also was significantly higher in high grade squamous intraepithelial lesion than in low grade squamous intraepithelial lesion and significantly lower in squamous cell carcinoma than in high grade intraepithelial lesion (p<0.01). These results suggested that mast cell number and percent of mast cell granulation were to be correlated to the development from precancerous lesion to cervical cancerous lesion and may be useful as markers of cervical squamous cell carcinoma.