복숭아혹진딧물 방제를 위한 Lecanicillium 균주 선발

정혜숙 ( Hye Sook Jung ),이향범 ( Hyang Burm Lee ),김근 ( Keun Kim ),이은영 ( Eun Young Lee ) 한국균학회 2006 韓國菌學會誌 Vol.34 No.2

數學科 二次函數 學習에서 애니메이션 方式 提示와 停止畵像 方式 提示가 學業 成就에 미치는 效果

정혜숙(Hye Sook Jung),한승록(Seung Rok Han) 공주대학교 교육연구소 1999 교육연구 Vol.15 No.-

This study aims at identifying the effects of presentation method using computer animation or static graphic by the learner’s cognitive style on the academic achievement of a quadratic function of mathematics. The quadratic function of mathematics is a learning task to understand the continuous relationship between variable x and y. Computer animation is considered a effective presentation method for understanding the quadratic function of mathematics because of its quality of motion and trajectory. The problem and hypotheses of the study were established as follows: Problem Ⅰ: which of presentation method, computer animation or static graphic, is more effective in the academic achievement of a quadratic function of mathematics. Hypothesis Ⅰ: There would be a significant difference in the academic achievement between computer animation method and static graphic one. Problem Ⅱ: In CAI, does the computer animation have any effects on the academic achievement according to learner’s cognitive style? Hypothesis Ⅱ-1: For the field-dependent learners, computer animation method would be the more effective than static graphic one in the academic achievement of a quadratic function of mathematics. Hypothesis Ⅱ-2: For the field-independent learners, there would be no significant differences in the academic achievement of a quadratic function of mathematics between computer animation method and static graphic one. To test above hypotheses, learning content was selected from the mathematics textbook for the 3rd grade middle school students. As experimental groups, two 2nd grade classes of a middle school located in Kongju city in Korea were selected. One was treated with courseware using computer animation method, the other was treated with courseware using static graphic one. The number of the subjects was 42 respectively. The results of this study were as follows: 1) The group with computer animation method showed significantly higher academic achievement than the one with static graphic method(p〈.05). 2) For the field-dependent learners, the group with computer animation method showed significantly higher academic achievement than the one with static graphic method(p〈.01). 3) For the field-independent learners, there were no significant differences in the academic achievement between computer animation method and static graphic one(p〉.05). On the basis of the results, the following conclusions could be made. The courseware using computer animation method was more effective in learning a quadratic function of mathematics than the one using static graphic method. For the field-dependent learners, the courseware using computer animation method showed significantly higher academic achievement than the one using static graphic method, but for the independent-learners, there were no differences between the two methods.

콩떡의 제조 및 저장과 포장에 따른 물성 변화와 관능적 특성

정혜숙 ( Hye Sook Jung ) 한국가정과학회 2002 한국가정과학회지 Vol.5 No.2

This study, observing each respectively packaged Kongdduk during 12-day storing period and comparing it with unpackaged Kongdduk, through a cross-sectional view of its fiber and temperament, through a sensory evaluation rheometer measurement of theological change depending on storing period and packaging type and through the organic acid content, micro-organic change, and retrogressive process. The results are as follows: 1. Kongdduk made of bean oil shows better chewiness, cohesiveness, and moistness than Kongdduk made of bean flour while Kongdduk made of bean flour shows better rheological properties as to roasted nutty taste or roasted nutty ordor. 2. The test of the cutted loaves of Kongdduk shows that adding oil of proper proportion to the dough of steamed rice cake in accordance with the amount of rice flour has a good influence on rheological properties of softness. 3. Rice cakes were prepared by addition of yellow soybean flour or peanut flour and packaged with CMP or VP, and their physical characteristics were monitored by sensory evaluation and mechanical measurement while storing for 6 days. For VP samples, yellow soybean rice cake showed little changes in cohesiveness, moistness and chewiness for 6 days of storage, while pure rice cake and peanut rice cake showed an increase in strength and hardness from the 4th day of storage. In case of CMP, yellow soybean rice cake hardly showed a difference in cohesiveness, moistness and chewiness for 6 days, while pure rice cake and peanut rice cake showed a significant difference from the 4th day in sensory evaluation. 4. For rheometer measurement, yellow soybean rice cake with CMP or VP showed little changes in strength or hardness for 6 days, while peanut rice cake and pure rice cake showed a drastic decrease in cohesiveness from the 2nd day and adhesiveness from the 4th day of storage. As there was no remarkable difference or deterioration for 6 days of storage in yellow soybean rice cake between CMP and VP, the ingredients of rice cakeappeared to be more important than the type of packaging in terms of quality deterioration of rice cake. 5. As the storing period passed by, organic acid is detected less at CMP-packaged Kongdduk than at wrapped, and its increasing speed proves to be slower as well. 6. The one wrapped with plyethylene film began to get moldy from pure rice cake or Kongdduk(rice cake mixed with yellow soybean or peanut) after 6 days, and more and more modly after 9 daysor after 12 days, but the CMP-packaged ones didn,t get modly until 12 days or more. 7. CMP-packaged Kongdduk showed higher enthalpy of retrogradation than PE-wrapped one. As storing period passed by, Kongdduk,s enthalpy grew high. That is to say, it shows that Kongdduk got retrograded.

Protein Engineering of Flavin-containing Monooxygenase from Corynebacterium glutamicum for Improved Production of Indigo and Indirubin

Hye Sook Jung(정혜숙),Hae Bin Jung(정혜빈),Hee Sook Kim(김희숙),Chang Gyeom Kim(김창겸),Jin Ho Lee(이진호) 한국생명과학회 2018 생명과학회지 Vol.28 No.6

향상된 인디고이드 생산능력을 갖는 코리네박테리움 유래의 변이 플라빈-함유 모노옥시게나제(cFMO)를 개발하기 위하여, cFMO 효소의 상동성모델을 이용하여 말토오스-결합단백질(MBP)과 융합된 4가지 변이체(F170Y, A210G, A210S, T326S)를 제작하고 그 생화학적 특징을 밝혔다. 정제된 MBP-T326S는 최적 활성을 위하여 야생형보다 고농도의 FAD (100 μM)를 요구하며, 100 μM의 FAD 첨가조건에서 kcat/Km이 3.8배 증가되었다. 인돌 옥시게나제 활성은 야생형의 63-77%를 나타냈다. MBP-T326S는 기질이 존재하지 않을 경우 쓸모없는 NADPH 산화효소활성이 매우 낮은 수준을 보여주었다(21-24%). T326S이외의 변이 단백질들은 야생형에 비하여 Km은 비슷하며 kcat/Km은 증가하였다. MBP-F170Y와 -A210S 변이단백질은 인돌 옥시게나제 활성이 각각 3.1배, 2.9배 증가하였다. 2.5 g/l의 트립토판을 함유한 LB배지에서 인디고이드 생산을 시험했을 때, 야생형 cFMO를 함유한 대장균은 684 mg/l의 인디고와 104 mg/l의 인디루빈을 생산한 반면, T326S를 함유한 세포는 1,040 mg/l의 인디고와 112 mg/l의 인디루빈을 생산하였다. 이전의 결과인 Methylophaga 유래의 FMO를 발현하는 대장균에서 가장 높은 수준인 920 mg/l의 인디고를 생산한 것과 비교하면, 본 연구결과는 인디고 생산이 13% 높은 수준이였다. 상동성 모델링에 기반한 cFMO의 단백질공학은 인디고이드 생산균을 개발하는데 보다 더 논리적인 전략을 제시하였다. Flavin-containing monooxygenases from Corynebacterium (cFMOs) were mutagenized based on homology modeling to develop variants with an enhanced indigoid production capability. The four mutants, F170Y, A210G, A210S, and T326S, which fused to a maltose-binding protein (MBP), were constructed, and their biochemical properties were characterized. Of these, purified MBP-T326S required a higher concentration of exogenous FAD (100 mM) than the wild-type MBP-cFMO for optimal activity and showed a 3.8-fold increase in the kcat/Km value at 100 μM FAD compared to that of MBP-cFMO at 2 μM FAD. The indole oxygenase activities of MBP-T326S decreased to 63-77% compared to that of the MBP-cFMO In addition, MBP-T326S displayed a very low level of futile NADPH oxidase activities (21-24%) in the absence of a substrate. Mutant proteins except for T326S displayed similar Km and increased kcat/Km values compared to the wild-type. MBP-F170Y and -A210S mutants showed elevated indole oxygenase activity higher than 3.1- and 2.9-fold, respectively, in comparison with MBP-cFMO. When indigoid production was carried out in LB broth with 2.5 g/l of tryptophan, Escherichia coli expressing cFMO produced 684 mg/l of indigo and 104 mg/l of indirubin, while cells harboring T326S produced 1,040 mg/l of indigo and 112 mg/l of indirubin. The results indicate that the production of indigo was 13% higher when compared to a previous report in which an E. coli expressing FMO from Methylophaga produced 920 mg/l of indigo. The protein engineering of cFMO based on homology modeling provided a more rational strategy for developing indigoid-producing strains.

부탄올 내성 미생물의 분리, 동정 및 변이주의 개발

정혜숙 ( Hye Sook Jung ),이진호 ( Jin Ho Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2013 한국미생물·생명공학회지 Vol.41 No.1

부탄올 용매에서 생존하는 부탄올 내성 미생물을 분리하였다. 분리된 미생물들의 세포성장은 부탄올 농도가 증가함에 따라 감소하였으며, 그 중에서 BRS02가 12.5 g/L에서 가장 높은 내성도를 나타내었다. 또한, UV를 이용하여 BRS02 균의 변이를 유도하여 고농도 부탄올 내성균 BRS251을 개발하였다. 부탄올 생산 모델균주로 대장균과 함께 부탄올, 프로판올 및 펜탄올에 대한 내성도를 비교한 결과, 대장균은 7.5 g/L 부탄올과 20 g/L 프로판올, 2 g/L 펜탄올 농도까지 생육이 가능한 반편, BRS251은 더 고농도인 17.5 g/L 부탄올과 32.5 g/L 프로판올, 6 g/L 펜탄올 농도까지 생육이 가능하였다. 분리된 세균을 동정하기 위해서 그람염색 후 광학현미경으로 관찰한 결과 그람양성의 구균으로 확인이 되었으며, 6.5% NaCl에서 생육이 가능하였다. 생화학적 특성을 분석한 결과, arginine dihydrolase, α-glucosidase, urease 효소활성을 가지고 있었으며, 호기적인 조건에서 D-galactose, Dmaltose, D-mannitol, D-mannose, methyl-β-D-glucopyranoside, D-ribose, sucrose, D-trehalose를 탄소원으로 자화하여 산을 생성할 수 있었으며, bacitracin, vibriostatic agent O/129 및 optochin에 대한 항생제 내성을 나타내었다. 16S rRNA 유전자 서열을 결정하고 계통발생도 분석을 통해 BRS02는 최종적으로 Staphylococcus sp.임을 동정하였다. Butanol-resistant bacteria were isolated from butanol solvent. The cell growth of isolated strains declined with increasing concentrations of butanol, and isolated strain BRS02 displayed more resistance to 12.5 g/L of butanol than other isolated strains. In addition, strain BRS251, which was resistant to even higher concentrations of butanol, was developed by the mutation of BRS02 using UV. BRS251 could grow in LB medium containing up to 17.5 g/L of butanol, 32.5 g/L of propanol, or 6 g/L of pentanol, whereas the control strain Escherichia coli was found to be tolerant to 7.5 g/L of butanol, 20 g/L of propanol, or 2 g/L of pentanol. The isolated BRS02, a Gram (+) bacterium seen to have a cocci form under the microscope, grew in 6.5% NaCl. According to biochemical tests, BRS02 can metabolize and produce acid with D-galactose, D-maltose, D-mannitol, D mannose, methyl-β-Dglucopyranoside, D-ribose, sucrose, or D-trehalose, as carbon sources. Also, this strain showed resistance to bacitracin, vibriostatic agent O/129, and optochin, alongside positive activities for arginine dihydrolase, α-glucosidase, and urease. The BRS02 strain was identified as Staphylococcus sp. by analyses of the 16S rRNA gene, phylogenetic tree, and biochemical tests.

만 3세 이야기 나누기 활동에 대한 실행연구

정혜숙(Jung, Hye-Suk),김숙령(Kim, Sook-Ryong) 한국어린이교육문화비평학회 2012 영유아교육과정연구 Vol.2 No.1

본 연구는 유아교육기관의 만 세 학급에서 이루어지는 구조적인 이야기 나누기 활동의 실태와 문제 점을 파악하고 개선 방안을 모색하여 실행하는 순환적 과정을 거치며 변화되는 과정을 연구하고자 하였다 본 연구는 대전에 위치한 유치원 내 만 세반인 달님반에서 이루어졌으며 연구 참여자는 이 학급 유아 명과 담임교사인 본 연구자이다 연구는 만 세 이야기 나누기 활동의 실태 및 문제점 파악 차실행 중간 점검 차 실행 중간 점검 차 실행 종합 점검 순으로 진행되었다 본 연구의 결과는 첫째 이야기 나누기 시간을 통해 교사는 유아들과 자유롭게 대화할 수 있는 분위기를 조성해야 하는데 교사 유아 간의 대화보다 유아 유아들 간의 대화의 비율을 더욱 높이기 위하여 다양한 자리 배치를 시도하였고 그 중에서 교사의 시선을 벗어나지 않고 유아 간 서로의 얼굴을 마주보며 대화할 수 있는 반원대형을 형성하게 되었다 둘째 유아들이 가진 작은 경험을 꺼내고 교사의 지식이 합쳐져 예전에 알고 있던 지식이 견고히 된다거나 혹은 새로운 지식이 성립될 수 있도록 이야기 주제 선정 시 유아의 입장을 충분히 고려하고 부족한 부분은 이야기 나누기 시간을 통해 경험할 수 있도록 수업을 계획하는 것이 효과 적이었다 셋째 어떤 이야기 나누기이든지 소통적인 요소도 가지고 있고 단절적인 요소도 가지고 있다 다만 주제에 따라 다를 수는 있지만 교사가 이야기나누기에 대한 배경지식이 얼마큼이며 소통에 대한 부분을 어느 정도 인식을 하고 있느냐에 따라 노력의 여하가 결정되며 이야기의 질 이야기 나누기 시간의 역동성 유아 참여도와 완성도 은 달라질 수 있다 The study aimed to understand the current condition and problems of the str4uctured story sharing activity which was implemented for 3 year old children in the child care institution and to implement this activity in an improved way. The participants of the research were 16 children who were 3 years old in Dalnimban of P kindergarten located in Daejeon and 1 homeroom teacher, the researcher. The current status and problems of the activity were explored and then the 1st implementation was made. After then, the second implementation, interim reviews, the third implementation and the final comprehensive review were carried out in order. The results of the research involve the following. First, During the sharing story time, a teacher is supposed to form an atmosphere in which they can freely converse, and the teacher attempted to make various placements in order to further enhance the ratio of conversations between younger children rather than between a teacher-younger children. Among others, the teacher created a semi-circular form in which younger children could converse face-to-face within the eyesight of the teacher. Second, it was effective that the class was planned considering the following points: when children s experiences could become stronger with an impact of the teacher s knowledge; when children s situation could be considered for the formation of the new knowledge when the subject is chosen; and when children could experience what the did not know before the class through sharing stories. Third, any story sharing has communicative factors and intermittent factors. However, it may be different in accordance with the topic, yet, the effort for story sharing can be determined according to how much background knowledge a teacher has concerning story sharing and how much a teacher recognizes a communicative part; in addition, the quality of a story can be different(the dynamics of a story sharing time and the participation rate of younger children and the degree of completion).

영아의 어린이집 적응 척도의 개발 및 타당화에 관한 연구

성영화(Sung Young-Hwa),정혜숙(Jung Hye-Sook),고태순(Koh Tae-Soon) 한국영유아보육학회 2011 한국영유아보육학 Vol.0 No.68

본 연구는 우리나라 12개월부터 36개월 영아의 어린이집 적응을 평가할 수 있는 도구를 개발하고 타당화 하는데 목적이 있다. 연구 목적을 위해 선행 연구들과 국내ㆍ외에서 사용하고 있는 영아 적응 관련 척도들을 분석하여 영아 적응 척도의 구성 요인들을 추출하였다. 이를 기초로 문항을 제작하고 구인 타당도 검증을 위해 탐색적 요인분석과 확인적 요인분석을 실시하였다. 탐색적 요인분석은 221명의 영아들을 연구대상으로 하였으며 확인적 요인분석은 241명의 영아들을 연구대상으로 하여 본 연구의 총 연구대상 영아들은 462명이었다. 최종 척도의 신뢰도를 살펴보기 위해 내적 합치도를 산출하였다. 탐색적 연구결과 친사회성, 긍정적 정서, 일과적응, 자아강도의 4개 요인으로 구성된 22문항의 영아적응 척도를 완성하였으며 확인적 요인분석을 통해 적합도 검증을 한 결과 다양한 부합도 지수가 적절하게 나타나 본 연구에서 개발한 척도가 타당한 도구임을 검증할 수 있었다. 신뢰도 검증 결과 척도의 전체 신뢰도는 .94였으며 각 요인별로 .80~.92의 신뢰도를 갖는 것으로 나타났다. The purpose of this study was to develop and validate an instrument to measure adjustment for young children in child care center. For this purpose, the literature relevant to adjustment for young children were reviewed and hypothetical constructs were extracted. Based on these constructs and content validity taking from opinions of experts in child care field, the items to measure adjustment of young children in child care center were created. The validity of the scale was tested through exploratory and confirmatory factor analysis. The subjects for exploratory factor analysis were 221 and the subjects for confirmatory factor analysis were 241. So, all subjects of this study were 462. From the exploratory factor analysis, a 22-item scale were developed. This scale was composed of 4 factors including pro-sociality, positive affect, adjustment to day care routines, ego-strength. Also this scale proved to be a verified scale from testing the confirmatory factor analysis. The reliability was tested through the internal consistency estimate by Cronbach’s alpha. The whole reliability of this scale was .94 and the reliability according to factors indicated .80~.92.

JESS 시스템을 이용한 특성 모델링 도구 구현

지은미(Eun-Mi Ji),정혜숙(Hye-Sook Jung),곽미선(Mi-Sun Kuak),최승훈(Seung-Hoon Choi) 한국정보과학회 2008 한국정보과학회 학술발표논문집 Vol.35 No.1

특성 모델(Feature Model)은 소프트웨어 제품 라인 개발 시 도메인 공학 단계에서 제품들 사이의 공통점과 차이점을 모델링하는데 널리 사용된다. 특성 구성(Feature Configuration)은 특성 모델로부터 특정제품에 포함될 특성들을 선택한 결과이다. 특성 구성은, 특성 모델에 표현되어 있는 여러 가지 제한 조건을 만족해야 한다. 본 논문에서는 특성 모델 작성 기능과 특성 구성 정의 기능을 지원하고 특성 구성의 검증 기능을 지식 기반 시스템인 JESS를 활용하여 구현한 특성 모델링 도구를 제안한다. 본 도구는 자바 언어와의 결합성이 좋은 JESS 시스템을 이용하여 확장성이 좋으며 특성 구성에서의 오류 원인을 명확히 알려주는 장점을 가진다.

Ubiquitous 기술 적용을 위한 건축 디자인 요소에 관한 연구

김억(Kim, Uk),이지현(Lee, Ji-Hyun),정혜숙(Jung, Hye-Sook) 한국도시설계학회 2008 도시설계 : 한국도시설계학회지 Vol.9 No.4

포장방법에 의한 콩떡의 저장 안정성에 관한 연구

정혜숙,김경자 동아시아식생활학회 2001 동아시아식생활학회지 Vol.11 No.3

The objective of this study consists in finding the ways to make soybean cake(which is made of soaked soybean flour containing protein and lipid) a scientific and practical food even more easily. This study took a measurement of the change of pH, organic acid, microorganism, retrogradation and so soon, observing soybean cake prepared with soybean flour containing 6% of soybean oil at room temperature (19℃) in two types of packaging. that is to say. CO₂ modified packing(CMP) and liner low density polyethylene(LLDPE) packaging. As storing time went by, packed soybean cake didn't appeared in 12 days, either. Using modified atmosphere packaging soybean cake showed higher pH as well as less organic acid than unpacked. In addition, mould method makes water-activity lower, and it puts a curb on the development of aerobic perishable microorganism and the retrogradation of rice cake. Unpacked soybean cake showed higher values than CMP Soybean Cake with enthalpy of retrogradation and the longer storing period the greater retrogradation process. Thus, storing or circulation period can be increased effectively without chemical or physical treatment.